id	sid	tid	token	lemma	pos
cord-009376-a35a92gh	1	1	key	key	JJ
cord-009376-a35a92gh	1	2	:	:	:
cord-009376-a35a92gh	1	3	cord-009376-a35a92gh	cord-009376-a35a92gh	NN
cord-009376-a35a92gh	1	4	authors	author	NNS
cord-009376-a35a92gh	1	5	:	:	:
cord-009376-a35a92gh	1	6	Lovatt	Lovatt	NNP
cord-009376-a35a92gh	1	7	,	,	,
cord-009376-a35a92gh	1	8	Archie	Archie	NNP
cord-009376-a35a92gh	1	9	title	title	NN
cord-009376-a35a92gh	1	10	:	:	:
cord-009376-a35a92gh	1	11	Applications	application	NNS
cord-009376-a35a92gh	1	12	of	of	IN
cord-009376-a35a92gh	1	13	quantitative	quantitative	JJ
cord-009376-a35a92gh	1	14	PCR	PCR	NNP
cord-009376-a35a92gh	1	15	in	in	IN
cord-009376-a35a92gh	1	16	the	the	DT
cord-009376-a35a92gh	1	17	biosafety	biosafety	NN
cord-009376-a35a92gh	1	18	and	and	CC
cord-009376-a35a92gh	1	19	genetic	genetic	JJ
cord-009376-a35a92gh	1	20	stability	stability	NN
cord-009376-a35a92gh	1	21	assessment	assessment	NN
cord-009376-a35a92gh	1	22	of	of	IN
cord-009376-a35a92gh	1	23	biotechnology	biotechnology	NN
cord-009376-a35a92gh	1	24	products	product	NNS
cord-009376-a35a92gh	1	25	date	date	NN
cord-009376-a35a92gh	1	26	:	:	:
cord-009376-a35a92gh	1	27	2002	2002	CD
cord-009376-a35a92gh	1	28	-	-	HYPH
cord-009376-a35a92gh	1	29	01	01	CD
cord-009376-a35a92gh	1	30	-	-	HYPH
cord-009376-a35a92gh	1	31	07	07	CD
cord-009376-a35a92gh	1	32	journal	journal	NN
cord-009376-a35a92gh	1	33	:	:	:
cord-009376-a35a92gh	1	34	nan	nan	NNP
cord-009376-a35a92gh	1	35	DOI	DOI	NNP
cord-009376-a35a92gh	1	36	:	:	:
cord-009376-a35a92gh	1	37	10.1016	10.1016	CD
cord-009376-a35a92gh	1	38	/	/	SYM
cord-009376-a35a92gh	1	39	s1389	s1389	NN
cord-009376-a35a92gh	1	40	-	-	HYPH
cord-009376-a35a92gh	1	41	0352(01)00043	0352(01)00043	NNP
cord-009376-a35a92gh	1	42	-	-	HYPH
cord-009376-a35a92gh	1	43	5	5	CD
cord-009376-a35a92gh	1	44	sha	sha	NN
cord-009376-a35a92gh	1	45	:	:	:
cord-009376-a35a92gh	1	46	b504b69c61f427bd6d8b7b5049bee0fd6b85592f	b504b69c61f427bd6d8b7b5049bee0fd6b85592f	NNP
cord-009376-a35a92gh	1	47	doc_id	doc_id	CD
cord-009376-a35a92gh	1	48	:	:	:
cord-009376-a35a92gh	2	1	9376	9376	CD
cord-009376-a35a92gh	2	2	cord_uid	cord_uid	NNS
cord-009376-a35a92gh	2	3	:	:	:
cord-009376-a35a92gh	3	1	a35a92gh	a35a92gh	NNP
cord-009376-a35a92gh	3	2	High	high	JJ
cord-009376-a35a92gh	3	3	throughput	throughput	NN
cord-009376-a35a92gh	3	4	screening	screening	NN
cord-009376-a35a92gh	3	5	,	,	,
cord-009376-a35a92gh	3	6	increased	increased	JJ
cord-009376-a35a92gh	3	7	accuracy	accuracy	NN
cord-009376-a35a92gh	3	8	and	and	CC
cord-009376-a35a92gh	3	9	the	the	DT
cord-009376-a35a92gh	3	10	coupling	coupling	NN
cord-009376-a35a92gh	3	11	of	of	IN
cord-009376-a35a92gh	3	12	real	real	JJ
cord-009376-a35a92gh	3	13	-	-	HYPH
cord-009376-a35a92gh	3	14	time	time	NN
cord-009376-a35a92gh	3	15	quantitative	quantitative	JJ
cord-009376-a35a92gh	3	16	PCR	pcr	NN
cord-009376-a35a92gh	3	17	(	(	-LRB-
cord-009376-a35a92gh	3	18	Q	Q	NNP
cord-009376-a35a92gh	3	19	-	-	HYPH
cord-009376-a35a92gh	3	20	PCR	PCR	NNP
cord-009376-a35a92gh	3	21	)	)	-RRB-
cord-009376-a35a92gh	3	22	to	to	IN
cord-009376-a35a92gh	3	23	robotic	robotic	JJ
cord-009376-a35a92gh	3	24	set	set	VB
cord-009376-a35a92gh	3	25	-	-	HYPH
cord-009376-a35a92gh	3	26	up	up	RP
cord-009376-a35a92gh	3	27	systems	system	NNS
cord-009376-a35a92gh	3	28	are	be	VBP
cord-009376-a35a92gh	3	29	beginning	begin	VBG
cord-009376-a35a92gh	3	30	to	to	TO
cord-009376-a35a92gh	3	31	revolutionise	revolutionise	VB
cord-009376-a35a92gh	3	32	biotechnology	biotechnology	NN
cord-009376-a35a92gh	3	33	.	.	.
cord-009376-a35a92gh	4	1	Applications	application	NNS
cord-009376-a35a92gh	4	2	of	of	IN
cord-009376-a35a92gh	4	3	Q	Q	NNP
cord-009376-a35a92gh	4	4	-	-	HYPH
cord-009376-a35a92gh	4	5	PCR	PCR	NNP
cord-009376-a35a92gh	4	6	within	within	IN
cord-009376-a35a92gh	4	7	biotechnology	biotechnology	NN
cord-009376-a35a92gh	4	8	are	be	VBP
cord-009376-a35a92gh	4	9	discussed	discuss	VBN
cord-009376-a35a92gh	4	10	with	with	IN
cord-009376-a35a92gh	4	11	particular	particular	JJ
cord-009376-a35a92gh	4	12	emphasis	emphasis	NN
cord-009376-a35a92gh	4	13	on	on	IN
cord-009376-a35a92gh	4	14	the	the	DT
cord-009376-a35a92gh	4	15	following	follow	VBG
cord-009376-a35a92gh	4	16	areas	area	NNS
cord-009376-a35a92gh	4	17	of	of	IN
cord-009376-a35a92gh	4	18	biosafety	biosafety	NN
cord-009376-a35a92gh	4	19	and	and	CC
cord-009376-a35a92gh	4	20	genetic	genetic	JJ
cord-009376-a35a92gh	4	21	stability	stability	NN
cord-009376-a35a92gh	4	22	testing	testing	NN
cord-009376-a35a92gh	4	23	:	:	:
cord-009376-a35a92gh	4	24	(	(	-LRB-
cord-009376-a35a92gh	4	25	a	a	LS
cord-009376-a35a92gh	4	26	)	)	-RRB-
cord-009376-a35a92gh	4	27	determination	determination	NN
cord-009376-a35a92gh	4	28	of	of	IN
cord-009376-a35a92gh	4	29	the	the	DT
cord-009376-a35a92gh	4	30	biodistribution	biodistribution	NN
cord-009376-a35a92gh	4	31	of	of	IN
cord-009376-a35a92gh	4	32	gene	gene	NN
cord-009376-a35a92gh	4	33	therapy	therapy	NN
cord-009376-a35a92gh	4	34	vectors	vector	NNS
cord-009376-a35a92gh	4	35	in	in	IN
cord-009376-a35a92gh	4	36	animals	animal	NNS
cord-009376-a35a92gh	4	37	;	;	,
cord-009376-a35a92gh	4	38	(	(	-LRB-
cord-009376-a35a92gh	4	39	b	b	NN
cord-009376-a35a92gh	4	40	)	)	-RRB-
cord-009376-a35a92gh	4	41	quantification	quantification	NN
cord-009376-a35a92gh	4	42	of	of	IN
cord-009376-a35a92gh	4	43	the	the	DT
cord-009376-a35a92gh	4	44	residual	residual	JJ
cord-009376-a35a92gh	4	45	DNA	dna	NN
cord-009376-a35a92gh	4	46	in	in	IN
cord-009376-a35a92gh	4	47	final	final	JJ
cord-009376-a35a92gh	4	48	product	product	NN
cord-009376-a35a92gh	4	49	therapeutics	therapeutic	NNS
cord-009376-a35a92gh	4	50	;	;	,
cord-009376-a35a92gh	4	51	(	(	-LRB-
cord-009376-a35a92gh	4	52	c	c	NN
cord-009376-a35a92gh	4	53	)	)	-RRB-
cord-009376-a35a92gh	4	54	detection	detection	NN
cord-009376-a35a92gh	4	55	of	of	IN
cord-009376-a35a92gh	4	56	viral	viral	JJ
cord-009376-a35a92gh	4	57	and	and	CC
cord-009376-a35a92gh	4	58	bacterial	bacterial	JJ
cord-009376-a35a92gh	4	59	nucleic	nucleic	JJ
cord-009376-a35a92gh	4	60	acid	acid	NN
cord-009376-a35a92gh	4	61	in	in	IN
cord-009376-a35a92gh	4	62	contaminated	contaminated	JJ
cord-009376-a35a92gh	4	63	cell	cell	NN
cord-009376-a35a92gh	4	64	banks	bank	NNS
cord-009376-a35a92gh	4	65	and	and	CC
cord-009376-a35a92gh	4	66	final	final	JJ
cord-009376-a35a92gh	4	67	products	product	NNS
cord-009376-a35a92gh	4	68	;	;	,
cord-009376-a35a92gh	4	69	(	(	-LRB-
cord-009376-a35a92gh	4	70	d	d	NN
cord-009376-a35a92gh	4	71	)	)	-RRB-
cord-009376-a35a92gh	4	72	quantification	quantification	NN
cord-009376-a35a92gh	4	73	of	of	IN
cord-009376-a35a92gh	4	74	the	the	DT
cord-009376-a35a92gh	4	75	level	level	NN
cord-009376-a35a92gh	4	76	of	of	IN
cord-009376-a35a92gh	4	77	virus	virus	NN
cord-009376-a35a92gh	4	78	removal	removal	NN
cord-009376-a35a92gh	4	79	in	in	IN
cord-009376-a35a92gh	4	80	process	process	NN
cord-009376-a35a92gh	4	81	validation	validation	NN
cord-009376-a35a92gh	4	82	viral	viral	JJ
cord-009376-a35a92gh	4	83	clearance	clearance	NN
cord-009376-a35a92gh	4	84	studies	study	NNS
cord-009376-a35a92gh	4	85	;	;	,
cord-009376-a35a92gh	4	86	(	(	-LRB-
cord-009376-a35a92gh	4	87	e	e	LS
cord-009376-a35a92gh	4	88	)	)	-RRB-
cord-009376-a35a92gh	4	89	specific	specific	JJ
cord-009376-a35a92gh	4	90	detection	detection	NN
cord-009376-a35a92gh	4	91	of	of	IN
cord-009376-a35a92gh	4	92	retroviral	retroviral	JJ
cord-009376-a35a92gh	4	93	RT	rt	NN
cord-009376-a35a92gh	4	94	activity	activity	NN
cord-009376-a35a92gh	4	95	in	in	IN
cord-009376-a35a92gh	4	96	vaccines	vaccine	NNS
cord-009376-a35a92gh	4	97	with	with	IN
cord-009376-a35a92gh	4	98	high	high	JJ
cord-009376-a35a92gh	4	99	sensitivity	sensitivity	NN
cord-009376-a35a92gh	4	100	;	;	:
cord-009376-a35a92gh	4	101	and	and	CC
cord-009376-a35a92gh	4	102	(	(	-LRB-
cord-009376-a35a92gh	4	103	f	f	NNP
cord-009376-a35a92gh	4	104	)	)	-RRB-
cord-009376-a35a92gh	4	105	transgene	transgene	NN
cord-009376-a35a92gh	4	106	copy	copy	NN
cord-009376-a35a92gh	4	107	number	number	NN
cord-009376-a35a92gh	4	108	determination	determination	NN
cord-009376-a35a92gh	4	109	for	for	IN
cord-009376-a35a92gh	4	110	monitoring	monitor	VBG
cord-009376-a35a92gh	4	111	genetic	genetic	JJ
cord-009376-a35a92gh	4	112	stability	stability	NN
cord-009376-a35a92gh	4	113	during	during	IN
cord-009376-a35a92gh	4	114	production	production	NN
cord-009376-a35a92gh	4	115	.	.	.
cord-009376-a35a92gh	5	1	Methods	method	NNS
cord-009376-a35a92gh	5	2	employed	employ	VBN
cord-009376-a35a92gh	5	3	for	for	IN
cord-009376-a35a92gh	5	4	Q	Q	NNP
cord-009376-a35a92gh	5	5	-	-	HYPH
cord-009376-a35a92gh	5	6	PCR	PCR	NNP
cord-009376-a35a92gh	5	7	assay	assay	NN
cord-009376-a35a92gh	5	8	validation	validation	NN
cord-009376-a35a92gh	5	9	as	as	IN
cord-009376-a35a92gh	5	10	required	require	VBN
cord-009376-a35a92gh	5	11	in	in	IN
cord-009376-a35a92gh	5	12	ICH	ICH	NNP
cord-009376-a35a92gh	5	13	Topic	Topic	NNP
cord-009376-a35a92gh	5	14	Q2A	Q2A	NNP
cord-009376-a35a92gh	5	15	Validation	validation	NN
cord-009376-a35a92gh	5	16	of	of	IN
cord-009376-a35a92gh	5	17	Analytical	Analytical	NNP
cord-009376-a35a92gh	5	18	Methods	Methods	NNPS
cord-009376-a35a92gh	5	19	:	:	:
cord-009376-a35a92gh	5	20	Definitions	definition	NNS
cord-009376-a35a92gh	5	21	and	and	CC
cord-009376-a35a92gh	5	22	Terminology	terminology	NN
cord-009376-a35a92gh	5	23	(	(	-LRB-
cord-009376-a35a92gh	5	24	1st	1st	JJ
cord-009376-a35a92gh	5	25	June	June	NNP
cord-009376-a35a92gh	5	26	1995	1995	CD
cord-009376-a35a92gh	5	27	)	)	-RRB-
cord-009376-a35a92gh	5	28	are	be	VBP
cord-009376-a35a92gh	5	29	also	also	RB
cord-009376-a35a92gh	5	30	reviewed	review	VBN
cord-009376-a35a92gh	5	31	.	.	.
cord-009376-a35a92gh	6	1	Real	real	JJ
cord-009376-a35a92gh	6	2	-	-	HYPH
cord-009376-a35a92gh	6	3	time	time	NN
cord-009376-a35a92gh	6	4	quantitative	quantitative	JJ
cord-009376-a35a92gh	6	5	polymerase	polymerase	NN
cord-009376-a35a92gh	6	6	chain	chain	NN
cord-009376-a35a92gh	6	7	reac	reac	NN
cord-009376-a35a92gh	6	8	-	-	HYPH
cord-009376-a35a92gh	6	9	Ž	Ž	NNP
cord-009376-a35a92gh	6	10	.	.	.
cord-009376-a35a92gh	7	1	tion	tion	NN
cord-009376-a35a92gh	7	2	Q	Q	NNP
cord-009376-a35a92gh	7	3	-	-	HYPH
cord-009376-a35a92gh	7	4	PCR	PCR	NNP
cord-009376-a35a92gh	7	5	has	have	VBZ
cord-009376-a35a92gh	7	6	revolutionised	revolutionise	VBN
cord-009376-a35a92gh	7	7	the	the	DT
cord-009376-a35a92gh	7	8	detection	detection	NN
cord-009376-a35a92gh	7	9	and	and	CC
cord-009376-a35a92gh	7	10	quantification	quantification	NN
cord-009376-a35a92gh	7	11	of	of	IN
cord-009376-a35a92gh	7	12	nucleic	nucleic	JJ
cord-009376-a35a92gh	7	13	acid	acid	NN
cord-009376-a35a92gh	7	14	and	and	CC
cord-009376-a35a92gh	7	15	retroviral	retroviral	NN
cord-009376-a35a92gh	7	16	reverse	reverse	NN
cord-009376-a35a92gh	7	17	transcriptase	transcriptase	NN
cord-009376-a35a92gh	7	18	because	because	IN
cord-009376-a35a92gh	7	19	of	of	IN
cord-009376-a35a92gh	7	20	fast	fast	JJ
cord-009376-a35a92gh	7	21	throughput	throughput	NN
cord-009376-a35a92gh	7	22	Ž	ž	NN
cord-009376-a35a92gh	7	23	capability	capability	NN
cord-009376-a35a92gh	7	24	Morris	Morris	NNP
cord-009376-a35a92gh	7	25	et	et	NNP
cord-009376-a35a92gh	7	26	al	al	NNP
cord-009376-a35a92gh	7	27	.	.	NNP
cord-009376-a35a92gh	7	28	,	,	,
cord-009376-a35a92gh	7	29	1996	1996	CD
cord-009376-a35a92gh	7	30	;	;	:
cord-009376-a35a92gh	7	31	Lovatt	Lovatt	NNP
cord-009376-a35a92gh	7	32	et	et	NNP
cord-009376-a35a92gh	7	33	al	al	NNP
cord-009376-a35a92gh	7	34	.	.	NNP
cord-009376-a35a92gh	7	35	,	,	,
cord-009376-a35a92gh	7	36	1999	1999	CD
cord-009376-a35a92gh	7	37	;	;	:
cord-009376-a35a92gh	7	38	 	 	_SP
cord-009376-a35a92gh	7	39	.	.	.
cord-009376-a35a92gh	8	1	Nitsche	Nitsche	NNP
cord-009376-a35a92gh	8	2	et	et	NNP
cord-009376-a35a92gh	8	3	al	al	NNP
cord-009376-a35a92gh	8	4	.	.	NNP
cord-009376-a35a92gh	8	5	,	,	,
cord-009376-a35a92gh	8	6	1999	1999	CD
cord-009376-a35a92gh	8	7	.	.	.
cord-009376-a35a92gh	9	1	The	the	DT
cord-009376-a35a92gh	9	2	incorporation	incorporation	NN
cord-009376-a35a92gh	9	3	of	of	IN
cord-009376-a35a92gh	9	4	this	this	DT
cord-009376-a35a92gh	9	5	bulk	bulk	JJ
cord-009376-a35a92gh	9	6	harvest	harvest	NN
cord-009376-a35a92gh	9	7	material	material	NN
cord-009376-a35a92gh	9	8	.	.	.
cord-009376-a35a92gh	10	1	During	during	IN
cord-009376-a35a92gh	10	2	amplification	amplification	NN
cord-009376-a35a92gh	10	3	with	with	IN
cord-009376-a35a92gh	10	4	target	target	NN
cord-009376-a35a92gh	10	5	-	-	HYPH
cord-009376-a35a92gh	10	6	specific	specific	JJ
cord-009376-a35a92gh	10	7	oligonucleotide	oligonucleotide	JJ
cord-009376-a35a92gh	10	8	primers	primer	NNS
cord-009376-a35a92gh	10	9	,	,	,
cord-009376-a35a92gh	10	10	a	a	DT
cord-009376-a35a92gh	10	11	fluorogenic	fluorogenic	JJ
cord-009376-a35a92gh	10	12	oligonucleotide	oligonucleotide	NN
cord-009376-a35a92gh	10	13	probe	probe	NN
cord-009376-a35a92gh	10	14	with	with	IN
cord-009376-a35a92gh	10	15	both	both	DT
cord-009376-a35a92gh	10	16	reporter	reporter	NN
cord-009376-a35a92gh	10	17	Ž	ž	NN
cord-009376-a35a92gh	10	18	.	.	.
cord-009376-a35a92gh	11	1	dye	dye	NN
cord-009376-a35a92gh	11	2	FAM	FAM	NNP
cord-009376-a35a92gh	11	3	,	,	,
cord-009376-a35a92gh	11	4	VIC	VIC	NNP
cord-009376-a35a92gh	11	5	,	,	,
cord-009376-a35a92gh	11	6	TET	TET	NNP
cord-009376-a35a92gh	11	7	,	,	,
cord-009376-a35a92gh	11	8	JOE	JOE	NNP
cord-009376-a35a92gh	11	9	,	,	,
cord-009376-a35a92gh	11	10	or	or	CC
cord-009376-a35a92gh	11	11	HEX	hex	NN
cord-009376-a35a92gh	11	12	and	and	CC
cord-009376-a35a92gh	11	13	Ž	ž	NN
cord-009376-a35a92gh	11	14	.	.	.
cord-009376-a35a92gh	12	1	quencher	quencher	NN
cord-009376-a35a92gh	12	2	dye	dye	NN
cord-009376-a35a92gh	12	3	TAMRA	TAMRA	NNP
cord-009376-a35a92gh	12	4	attached	attach	VBN
cord-009376-a35a92gh	12	5	,	,	,
cord-009376-a35a92gh	12	6	anneals	anneal	NNS
cord-009376-a35a92gh	12	7	specifically	specifically	RB
cord-009376-a35a92gh	12	8	to	to	IN
cord-009376-a35a92gh	12	9	the	the	DT
cord-009376-a35a92gh	12	10	amplified	amplify	VBN
cord-009376-a35a92gh	12	11	product	product	NN
cord-009376-a35a92gh	12	12	between	between	IN
cord-009376-a35a92gh	12	13	the	the	DT
cord-009376-a35a92gh	12	14	amplimers	amplimer	NNS
cord-009376-a35a92gh	12	15	.	.	.
cord-009376-a35a92gh	13	1	The	the	DT
cord-009376-a35a92gh	13	2	detection	detection	NN
cord-009376-a35a92gh	13	3	reaction	reaction	NN
cord-009376-a35a92gh	13	4	utilises	utilise	VBZ
cord-009376-a35a92gh	13	5	the	the	DT
cord-009376-a35a92gh	13	6	5Ј	5ј	CD
cord-009376-a35a92gh	13	7	exonuclease	exonuclease	NN
cord-009376-a35a92gh	13	8	activity	activity	NN
cord-009376-a35a92gh	13	9	of	of	IN
cord-009376-a35a92gh	13	10	AmpliTaq	AmpliTaq	NNP
cord-009376-a35a92gh	13	11	Gold	Gold	NNP
cord-009376-a35a92gh	13	12	TM	TM	NNP
cord-009376-a35a92gh	13	13	DNA	dna	NN
cord-009376-a35a92gh	13	14	polymerase	polymerase	NN
cord-009376-a35a92gh	13	15	to	to	TO
cord-009376-a35a92gh	13	16	cleave	cleave	VB
cord-009376-a35a92gh	13	17	the	the	DT
cord-009376-a35a92gh	13	18	reporter	reporter	NN
cord-009376-a35a92gh	13	19	dye	dye	NN
cord-009376-a35a92gh	13	20	from	from	IN
cord-009376-a35a92gh	13	21	the	the	DT
cord-009376-a35a92gh	13	22	probe	probe	NN
cord-009376-a35a92gh	13	23	,	,	,
cord-009376-a35a92gh	13	24	which	which	WDT
cord-009376-a35a92gh	13	25	results	result	VBZ
cord-009376-a35a92gh	13	26	in	in	IN
cord-009376-a35a92gh	13	27	an	an	DT
cord-009376-a35a92gh	13	28	increased	increased	JJ
cord-009376-a35a92gh	13	29	fluorescence	fluorescence	NN
cord-009376-a35a92gh	13	30	Ž	ž	NN
cord-009376-a35a92gh	13	31	.	.	.
cord-009376-a35a92gh	14	1	when	when	WRB
cord-009376-a35a92gh	14	2	target	target	NN
cord-009376-a35a92gh	14	3	is	be	VBZ
cord-009376-a35a92gh	14	4	present	present	JJ
cord-009376-a35a92gh	14	5	Fig	Fig	NNP
cord-009376-a35a92gh	14	6	.	.	.
cord-009376-a35a92gh	14	7	1a	1a	CD
cord-009376-a35a92gh	14	8	.	.	.
cord-009376-a35a92gh	15	1	A	a	DT
cord-009376-a35a92gh	15	2	sequence	sequence	NN
cord-009376-a35a92gh	15	3	detector	detector	NN
cord-009376-a35a92gh	15	4	instrument	instrument	NN
cord-009376-a35a92gh	15	5	calculates	calculate	VBZ
cord-009376-a35a92gh	15	6	a	a	DT
cord-009376-a35a92gh	15	7	reporter	reporter	NN
cord-009376-a35a92gh	15	8	dye	dye	NN
cord-009376-a35a92gh	15	9	value	value	NN
cord-009376-a35a92gh	15	10	Ž	Ž	NNP
cord-009376-a35a92gh	15	11	.	.	.
cord-009376-a35a92gh	16	1	R	r	NN
cord-009376-a35a92gh	16	2	for	for	IN
cord-009376-a35a92gh	16	3	each	each	DT
cord-009376-a35a92gh	16	4	sample	sample	NN
cord-009376-a35a92gh	16	5	during	during	IN
cord-009376-a35a92gh	16	6	each	each	DT
cord-009376-a35a92gh	16	7	cycle	cycle	NN
cord-009376-a35a92gh	16	8	of	of	IN
cord-009376-a35a92gh	16	9	amplin	amplin	NNP
cord-009376-a35a92gh	16	10	fication	fication	NNP
cord-009376-a35a92gh	16	11	.	.	.
cord-009376-a35a92gh	17	1	The	the	DT
cord-009376-a35a92gh	17	2	value	value	NN
cord-009376-a35a92gh	17	3	of	of	IN
cord-009376-a35a92gh	17	4	R	r	NN
cord-009376-a35a92gh	17	5	,	,	,
cord-009376-a35a92gh	17	6	or	or	CC
cord-009376-a35a92gh	17	7	the	the	DT
cord-009376-a35a92gh	17	8	normalised	normalised	JJ
cord-009376-a35a92gh	17	9	ren	ren	NNP
cord-009376-a35a92gh	17	10	porter	porter	NNP
cord-009376-a35a92gh	17	11	signal	signal	NN
cord-009376-a35a92gh	17	12	,	,	,
cord-009376-a35a92gh	17	13	represents	represent	VBZ
cord-009376-a35a92gh	17	14	the	the	DT
cord-009376-a35a92gh	17	15	fluorescence	fluorescence	NN
cord-009376-a35a92gh	17	16	of	of	IN
cord-009376-a35a92gh	17	17	the	the	DT
cord-009376-a35a92gh	17	18	reporter	reporter	NN
cord-009376-a35a92gh	17	19	dye	dye	NN
cord-009376-a35a92gh	17	20	divided	divide	VBN
cord-009376-a35a92gh	17	21	by	by	IN
cord-009376-a35a92gh	17	22	a	a	DT
cord-009376-a35a92gh	17	23	passive	passive	JJ
cord-009376-a35a92gh	17	24	reference	reference	NN
cord-009376-a35a92gh	17	25	dye	dye	NN
cord-009376-a35a92gh	17	26	.	.	.
cord-009376-a35a92gh	18	1	During	during	IN
cord-009376-a35a92gh	18	2	PCR	PCR	NNP
cord-009376-a35a92gh	18	3	,	,	,
cord-009376-a35a92gh	18	4	R	r	NN
cord-009376-a35a92gh	18	5	increases	increase	VBZ
cord-009376-a35a92gh	18	6	as	as	IN
cord-009376-a35a92gh	18	7	the	the	DT
cord-009376-a35a92gh	18	8	amplicon	amplicon	NN
cord-009376-a35a92gh	18	9	copy	copy	NN
cord-009376-a35a92gh	18	10	n	n	CD
cord-009376-a35a92gh	18	11	number	number	NN
cord-009376-a35a92gh	18	12	increases	increase	NNS
cord-009376-a35a92gh	18	13	until	until	IN
cord-009376-a35a92gh	18	14	the	the	DT
cord-009376-a35a92gh	18	15	reaction	reaction	NN
cord-009376-a35a92gh	18	16	approaches	approach	VBZ
cord-009376-a35a92gh	18	17	a	a	DT
cord-009376-a35a92gh	18	18	plateau	plateau	NN
cord-009376-a35a92gh	18	19	.	.	.
cord-009376-a35a92gh	19	1	The	the	DT
cord-009376-a35a92gh	19	2	value	value	NN
cord-009376-a35a92gh	19	3	⌬	⌬	NNPS
cord-009376-a35a92gh	19	4	R	r	NN
cord-009376-a35a92gh	19	5	represents	represent	VBZ
cord-009376-a35a92gh	19	6	the	the	DT
cord-009376-a35a92gh	19	7	norn	norn	NNP
cord-009376-a35a92gh	19	8	Ž	Ž	NNP
cord-009376-a35a92gh	19	9	.	.	.
cord-009376-a35a92gh	20	1	malised	malised	NNP
cord-009376-a35a92gh	20	2	reporter	reporter	NN
cord-009376-a35a92gh	20	3	signal	signal	NN
cord-009376-a35a92gh	20	4	R	R	NNP
cord-009376-a35a92gh	20	5	minus	minus	IN
cord-009376-a35a92gh	20	6	the	the	DT
cord-009376-a35a92gh	20	7	baseline	baseline	JJ
cord-009376-a35a92gh	20	8	n	n	NNP
cord-009376-a35a92gh	20	9	signal	signal	NN
cord-009376-a35a92gh	20	10	established	establish	VBN
cord-009376-a35a92gh	20	11	in	in	IN
cord-009376-a35a92gh	20	12	the	the	DT
cord-009376-a35a92gh	20	13	first	first	JJ
cord-009376-a35a92gh	20	14	few	few	JJ
cord-009376-a35a92gh	20	15	cycles	cycle	NNS
cord-009376-a35a92gh	20	16	of	of	IN
cord-009376-a35a92gh	20	17	PCR	PCR	NNP
cord-009376-a35a92gh	20	18	.	.	.
cord-009376-a35a92gh	21	1	Like	like	IN
cord-009376-a35a92gh	21	2	R	r	NN
cord-009376-a35a92gh	21	3	,	,	,
cord-009376-a35a92gh	21	4	⌬	⌬	NNP
cord-009376-a35a92gh	21	5	R	r	NN
cord-009376-a35a92gh	21	6	increases	increase	NNS
cord-009376-a35a92gh	21	7	during	during	IN
cord-009376-a35a92gh	21	8	PCR	PCR	NNP
cord-009376-a35a92gh	21	9	as	as	IN
cord-009376-a35a92gh	21	10	the	the	DT
cord-009376-a35a92gh	21	11	amn	amn	NNP
cord-009376-a35a92gh	21	12	n	n	NNP
cord-009376-a35a92gh	21	13	plicon	plicon	NNP
cord-009376-a35a92gh	21	14	copy	copy	NN
cord-009376-a35a92gh	21	15	number	number	NN
cord-009376-a35a92gh	21	16	increases	increase	NNS
cord-009376-a35a92gh	21	17	until	until	IN
cord-009376-a35a92gh	21	18	the	the	DT
cord-009376-a35a92gh	21	19	reaction	reaction	NN
cord-009376-a35a92gh	21	20	Ž	Ž	NNP
cord-009376-a35a92gh	21	21	.	.	.
cord-009376-a35a92gh	22	1	reaches	reach	VBZ
cord-009376-a35a92gh	22	2	a	a	DT
cord-009376-a35a92gh	22	3	plateau	plateau	NN
cord-009376-a35a92gh	22	4	Fig	fig	NN
cord-009376-a35a92gh	22	5	.	.	.
cord-009376-a35a92gh	23	1	1b	1b	LS
cord-009376-a35a92gh	23	2	.	.	.
cord-009376-a35a92gh	24	1	The	the	DT
cord-009376-a35a92gh	24	2	use	use	NN
cord-009376-a35a92gh	24	3	of	of	IN
cord-009376-a35a92gh	24	4	a	a	DT
cord-009376-a35a92gh	24	5	sequence	sequence	NN
cord-009376-a35a92gh	24	6	detector	detector	NN
cord-009376-a35a92gh	24	7	allows	allow	VBZ
cord-009376-a35a92gh	24	8	measurement	measurement	NN
cord-009376-a35a92gh	24	9	of	of	IN
cord-009376-a35a92gh	24	10	the	the	DT
cord-009376-a35a92gh	24	11	amplified	amplify	VBN
cord-009376-a35a92gh	24	12	product	product	NN
cord-009376-a35a92gh	24	13	in	in	IN
cord-009376-a35a92gh	24	14	direct	direct	JJ
cord-009376-a35a92gh	24	15	proportion	proportion	NN
cord-009376-a35a92gh	24	16	to	to	IN
cord-009376-a35a92gh	24	17	the	the	DT
cord-009376-a35a92gh	24	18	increase	increase	NN
cord-009376-a35a92gh	24	19	in	in	IN
cord-009376-a35a92gh	24	20	fluorescence	fluorescence	NN
cord-009376-a35a92gh	24	21	emission	emission	NN
cord-009376-a35a92gh	24	22	continuously	continuously	RB
cord-009376-a35a92gh	24	23	during	during	IN
cord-009376-a35a92gh	24	24	PCR	PCR	NNP
cord-009376-a35a92gh	24	25	Ž	Ž	NNP
cord-009376-a35a92gh	24	26	.	.	.
cord-009376-a35a92gh	24	27	amplification	amplification	NN
cord-009376-a35a92gh	24	28	.	.	.
cord-009376-a35a92gh	25	1	The	the	DT
cord-009376-a35a92gh	25	2	threshold	threshold	NN
cord-009376-a35a92gh	25	3	cycle	cycle	NN
cord-009376-a35a92gh	25	4	C	c	NN
cord-009376-a35a92gh	25	5	value	value	NN
cord-009376-a35a92gh	25	6	is	be	VBZ
cord-009376-a35a92gh	25	7	T	T	NNP
cord-009376-a35a92gh	25	8	calculated	calculate	VBN
cord-009376-a35a92gh	25	9	in	in	IN
cord-009376-a35a92gh	25	10	real	real	JJ
cord-009376-a35a92gh	25	11	time	time	NN
cord-009376-a35a92gh	25	12	and	and	CC
cord-009376-a35a92gh	25	13	is	be	VBZ
cord-009376-a35a92gh	25	14	defined	define	VBN
cord-009376-a35a92gh	25	15	as	as	IN
cord-009376-a35a92gh	25	16	the	the	DT
cord-009376-a35a92gh	25	17	PCR	PCR	NNP
cord-009376-a35a92gh	25	18	cycle	cycle	NN
cord-009376-a35a92gh	25	19	number	number	NN
cord-009376-a35a92gh	25	20	at	at	IN
cord-009376-a35a92gh	25	21	which	which	WDT
cord-009376-a35a92gh	25	22	an	an	DT
cord-009376-a35a92gh	25	23	increase	increase	NN
cord-009376-a35a92gh	25	24	in	in	IN
cord-009376-a35a92gh	25	25	reporter	reporter	NN
cord-009376-a35a92gh	25	26	fluorescence	fluorescence	NN
cord-009376-a35a92gh	25	27	above	above	IN
cord-009376-a35a92gh	25	28	the	the	DT
cord-009376-a35a92gh	25	29	baseline	baseline	JJ
cord-009376-a35a92gh	25	30	signal	signal	NN
cord-009376-a35a92gh	25	31	can	can	MD
cord-009376-a35a92gh	25	32	first	first	RB
cord-009376-a35a92gh	25	33	be	be	VB
cord-009376-a35a92gh	25	34	detected	detect	VBN
cord-009376-a35a92gh	25	35	.	.	.
cord-009376-a35a92gh	26	1	Therefore	therefore	RB
cord-009376-a35a92gh	26	2	the	the	DT
cord-009376-a35a92gh	26	3	cycling	cycling	NN
cord-009376-a35a92gh	26	4	point	point	NN
cord-009376-a35a92gh	26	5	of	of	IN
cord-009376-a35a92gh	26	6	a	a	DT
cord-009376-a35a92gh	26	7	given	give	VBN
cord-009376-a35a92gh	26	8	amplification	amplification	NN
cord-009376-a35a92gh	26	9	where	where	WRB
cord-009376-a35a92gh	26	10	a	a	DT
cord-009376-a35a92gh	26	11	significant	significant	JJ
cord-009376-a35a92gh	26	12	increase	increase	NN
cord-009376-a35a92gh	26	13	in	in	IN
cord-009376-a35a92gh	26	14	the	the	DT
cord-009376-a35a92gh	26	15	fluorescence	fluorescence	NN
cord-009376-a35a92gh	26	16	signal	signal	NN
cord-009376-a35a92gh	26	17	associated	associate	VBN
cord-009376-a35a92gh	26	18	with	with	IN
cord-009376-a35a92gh	26	19	exponential	exponential	JJ
cord-009376-a35a92gh	26	20	growth	growth	NN
cord-009376-a35a92gh	26	21	of	of	IN
cord-009376-a35a92gh	26	22	the	the	DT
cord-009376-a35a92gh	26	23	PCR	PCR	NNP
cord-009376-a35a92gh	26	24	product	product	NN
cord-009376-a35a92gh	26	25	is	be	VBZ
cord-009376-a35a92gh	26	26	first	first	RB
cord-009376-a35a92gh	26	27	detected	detect	VBN
cord-009376-a35a92gh	26	28	,	,	,
cord-009376-a35a92gh	26	29	rep	rep	NNP
cord-009376-a35a92gh	26	30	-	-	HYPH
cord-009376-a35a92gh	26	31	Ž	Ž	NNP
cord-009376-a35a92gh	26	32	resents	resent	VBZ
cord-009376-a35a92gh	26	33	the	the	DT
cord-009376-a35a92gh	26	34	C	C	NNP
cord-009376-a35a92gh	26	35	value	value	NN
cord-009376-a35a92gh	26	36	of	of	IN
cord-009376-a35a92gh	26	37	that	that	DT
cord-009376-a35a92gh	26	38	amplification	amplification	NN
cord-009376-a35a92gh	26	39	Fig	Fig	NNP
cord-009376-a35a92gh	26	40	.	.	.
cord-009376-a35a92gh	27	1	T	T	NNP
cord-009376-a35a92gh	27	2	.	.	.
cord-009376-a35a92gh	28	1	1b	1b	LS
cord-009376-a35a92gh	28	2	.	.	.
cord-009376-a35a92gh	29	1	A	a	DT
cord-009376-a35a92gh	29	2	linear	linear	JJ
cord-009376-a35a92gh	29	3	relationship	relationship	NN
cord-009376-a35a92gh	29	4	exists	exist	VBZ
cord-009376-a35a92gh	29	5	between	between	IN
cord-009376-a35a92gh	29	6	the	the	DT
cord-009376-a35a92gh	29	7	C	C	NNP
cord-009376-a35a92gh	29	8	T	T	NNP
cord-009376-a35a92gh	29	9	value	value	NN
cord-009376-a35a92gh	29	10	and	and	CC
cord-009376-a35a92gh	29	11	starting	start	VBG
cord-009376-a35a92gh	29	12	quantity	quantity	NN
cord-009376-a35a92gh	29	13	of	of	IN
cord-009376-a35a92gh	29	14	target	target	NN
cord-009376-a35a92gh	29	15	molecule	molecule	NN
cord-009376-a35a92gh	29	16	,	,	,
cord-009376-a35a92gh	29	17	allowing	allow	VBG
cord-009376-a35a92gh	29	18	the	the	DT
cord-009376-a35a92gh	29	19	construction	construction	NN
cord-009376-a35a92gh	29	20	of	of	IN
cord-009376-a35a92gh	29	21	a	a	DT
cord-009376-a35a92gh	29	22	standard	standard	JJ
cord-009376-a35a92gh	29	23	curve	curve	NN
cord-009376-a35a92gh	29	24	.	.	.
cord-009376-a35a92gh	30	1	The	the	DT
cord-009376-a35a92gh	30	2	greater	great	JJR
cord-009376-a35a92gh	30	3	the	the	DT
cord-009376-a35a92gh	30	4	amount	amount	NN
cord-009376-a35a92gh	30	5	of	of	IN
cord-009376-a35a92gh	30	6	target	target	NN
cord-009376-a35a92gh	30	7	copies	copy	NNS
cord-009376-a35a92gh	30	8	in	in	IN
cord-009376-a35a92gh	30	9	the	the	DT
cord-009376-a35a92gh	30	10	Ž	ž	NN
cord-009376-a35a92gh	30	11	.Ž	.Ž	NNP
cord-009376-a35a92gh	30	12	reaction	reaction	NN
cord-009376-a35a92gh	30	13	,	,	,
cord-009376-a35a92gh	30	14	the	the	DT
cord-009376-a35a92gh	30	15	lower	low	JJR
cord-009376-a35a92gh	30	16	the	the	DT
cord-009376-a35a92gh	30	17	C	C	NNP
cord-009376-a35a92gh	30	18	value	value	NN
cord-009376-a35a92gh	30	19	Fig	Fig	NNP
cord-009376-a35a92gh	30	20	.	.	.
cord-009376-a35a92gh	30	21	2	2	CD
cord-009376-a35a92gh	30	22	Heid	Heid	NNP
cord-009376-a35a92gh	30	23	et	et	NNP
cord-009376-a35a92gh	30	24	T	t	NN
cord-009376-a35a92gh	30	25	.	.	.
cord-009376-a35a92gh	31	1	al	al	NNP
cord-009376-a35a92gh	31	2	.	.	NNP
cord-009376-a35a92gh	31	3	,	,	,
cord-009376-a35a92gh	31	4	1996	1996	CD
cord-009376-a35a92gh	31	5	;	;	:
cord-009376-a35a92gh	31	6	Livak	Livak	NNP
cord-009376-a35a92gh	31	7	and	and	CC
cord-009376-a35a92gh	31	8	Perkin	Perkin	NNP
cord-009376-a35a92gh	31	9	Elmer	Elmer	NNP
cord-009376-a35a92gh	31	10	,	,	,
cord-009376-a35a92gh	31	11	1998	1998	CD
cord-009376-a35a92gh	31	12	.	.	.
cord-009376-a35a92gh	32	1	There	there	EX
cord-009376-a35a92gh	32	2	are	be	VBP
cord-009376-a35a92gh	32	3	many	many	JJ
cord-009376-a35a92gh	32	4	advantages	advantage	NNS
cord-009376-a35a92gh	32	5	of	of	IN
cord-009376-a35a92gh	32	6	real	real	JJ
cord-009376-a35a92gh	32	7	time	time	NN
cord-009376-a35a92gh	32	8	Q	q	NN
cord-009376-a35a92gh	32	9	-	-	HYPH
cord-009376-a35a92gh	32	10	PCR	PCR	NNP
cord-009376-a35a92gh	32	11	over	over	IN
cord-009376-a35a92gh	32	12	conventional	conventional	JJ
cord-009376-a35a92gh	32	13	end	end	NN
cord-009376-a35a92gh	32	14	-	-	HYPH
cord-009376-a35a92gh	32	15	point	point	NN
cord-009376-a35a92gh	32	16	PCR	PCR	NNP
cord-009376-a35a92gh	32	17	.	.	.
cord-009376-a35a92gh	33	1	For	for	IN
cord-009376-a35a92gh	33	2	example	example	NN
cord-009376-a35a92gh	33	3	,	,	,
cord-009376-a35a92gh	33	4	real	real	JJ
cord-009376-a35a92gh	33	5	-	-	HYPH
cord-009376-a35a92gh	33	6	time	time	NN
cord-009376-a35a92gh	33	7	Q	q	NN
cord-009376-a35a92gh	33	8	-	-	HYPH
cord-009376-a35a92gh	33	9	PCR	PCR	NNP
cord-009376-a35a92gh	33	10	is	be	VBZ
cord-009376-a35a92gh	33	11	performed	perform	VBN
cord-009376-a35a92gh	33	12	in	in	IN
cord-009376-a35a92gh	33	13	a	a	DT
cord-009376-a35a92gh	33	14	closed	closed	JJ
cord-009376-a35a92gh	33	15	reaction	reaction	NN
cord-009376-a35a92gh	33	16	system	system	NN
cord-009376-a35a92gh	33	17	and	and	CC
cord-009376-a35a92gh	33	18	eliminates	eliminate	VBZ
cord-009376-a35a92gh	33	19	the	the	DT
cord-009376-a35a92gh	33	20	post	post	JJ
cord-009376-a35a92gh	33	21	-	-	JJ
cord-009376-a35a92gh	33	22	PCR	pcr	JJ
cord-009376-a35a92gh	33	23	processing	processing	NN
cord-009376-a35a92gh	33	24	of	of	IN
cord-009376-a35a92gh	33	25	PCR	PCR	NNP
cord-009376-a35a92gh	33	26	products	product	NNS
cord-009376-a35a92gh	33	27	,	,	,
cord-009376-a35a92gh	33	28	thereby	thereby	RB
cord-009376-a35a92gh	33	29	increasing	increase	VBG
cord-009376-a35a92gh	33	30	throughput	throughput	NN
cord-009376-a35a92gh	33	31	and	and	CC
cord-009376-a35a92gh	33	32	reducing	reduce	VBG
cord-009376-a35a92gh	33	33	the	the	DT
cord-009376-a35a92gh	33	34	chances	chance	NNS
cord-009376-a35a92gh	33	35	of	of	IN
cord-009376-a35a92gh	33	36	carryover	carryover	NN
cord-009376-a35a92gh	33	37	con	con	NN
cord-009376-a35a92gh	33	38	-	-	HYPH
cord-009376-a35a92gh	33	39	tamination	tamination	NN
cord-009376-a35a92gh	33	40	.	.	.
cord-009376-a35a92gh	34	1	Moreover	moreover	RB
cord-009376-a35a92gh	34	2	,	,	,
cord-009376-a35a92gh	34	3	the	the	DT
cord-009376-a35a92gh	34	4	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	34	5	Q	Q	NNP
cord-009376-a35a92gh	34	6	-	-	HYPH
cord-009376-a35a92gh	34	7	PCR	PCR	NNP
cord-009376-a35a92gh	34	8	system	system	NN
cord-009376-a35a92gh	34	9	incorporates	incorporate	VBZ
cord-009376-a35a92gh	34	10	a	a	DT
cord-009376-a35a92gh	34	11	chemical	chemical	NN
cord-009376-a35a92gh	34	12	system	system	NN
cord-009376-a35a92gh	34	13	termed	term	VBN
cord-009376-a35a92gh	34	14	Am	Am	NNP
cord-009376-a35a92gh	34	15	-	-	HYPH
cord-009376-a35a92gh	34	16	pErase	pErase	NNP
cord-009376-a35a92gh	34	17	to	to	TO
cord-009376-a35a92gh	34	18	avoid	avoid	VB
cord-009376-a35a92gh	34	19	amplicon	amplicon	NN
cord-009376-a35a92gh	34	20	contamination	contamination	NN
cord-009376-a35a92gh	34	21	.	.	.
cord-009376-a35a92gh	35	1	Like	like	IN
cord-009376-a35a92gh	35	2	conventional	conventional	JJ
cord-009376-a35a92gh	35	3	qualitative	qualitative	JJ
cord-009376-a35a92gh	35	4	PCR	pcr	NN
cord-009376-a35a92gh	35	5	assays	assay	NNS
cord-009376-a35a92gh	35	6	,	,	,
cord-009376-a35a92gh	35	7	Q	Q	NNP
cord-009376-a35a92gh	35	8	-	-	HYPH
cord-009376-a35a92gh	35	9	PCR	PCR	NNP
cord-009376-a35a92gh	35	10	assays	assay	NNS
cord-009376-a35a92gh	35	11	should	should	MD
cord-009376-a35a92gh	35	12	operate	operate	VB
cord-009376-a35a92gh	35	13	under	under	IN
cord-009376-a35a92gh	35	14	a	a	DT
cord-009376-a35a92gh	35	15	strict	strict	JJ
cord-009376-a35a92gh	35	16	contamination	contamination	NN
cord-009376-a35a92gh	35	17	Ž	ž	NN
cord-009376-a35a92gh	35	18	control	control	NN
cord-009376-a35a92gh	35	19	system	system	NN
cord-009376-a35a92gh	35	20	i.e.	i.e.	IN
cord-009376-a35a92gh	35	21	separate	separate	JJ
cord-009376-a35a92gh	35	22	air	air	NN
cord-009376-a35a92gh	35	23	spaces	space	NNS
cord-009376-a35a92gh	35	24	for	for	IN
cord-009376-a35a92gh	35	25	all	all	DT
cord-009376-a35a92gh	35	26	.	.	.
cord-009376-a35a92gh	36	1	reactions	reaction	NNS
cord-009376-a35a92gh	36	2	to	to	TO
cord-009376-a35a92gh	36	3	give	give	VB
cord-009376-a35a92gh	36	4	increased	increased	JJ
cord-009376-a35a92gh	36	5	assurance	assurance	NN
cord-009376-a35a92gh	36	6	of	of	IN
cord-009376-a35a92gh	36	7	re	re	NN
cord-009376-a35a92gh	36	8	-	-	JJ
cord-009376-a35a92gh	36	9	avoiding	avoid	VBG
cord-009376-a35a92gh	36	10	cross	cross	NN
cord-009376-a35a92gh	36	11	-	-	NN
cord-009376-a35a92gh	36	12	contamination	contamination	NN
cord-009376-a35a92gh	36	13	.	.	.
cord-009376-a35a92gh	37	1	Because	because	IN
cord-009376-a35a92gh	37	2	of	of	IN
cord-009376-a35a92gh	37	3	the	the	DT
cord-009376-a35a92gh	37	4	increased	increase	VBN
cord-009376-a35a92gh	37	5	throughput	throughput	NN
cord-009376-a35a92gh	37	6	of	of	IN
cord-009376-a35a92gh	37	7	real	real	JJ
cord-009376-a35a92gh	37	8	time	time	NN
cord-009376-a35a92gh	37	9	Q	q	NN
cord-009376-a35a92gh	37	10	-	-	HYPH
cord-009376-a35a92gh	37	11	PCR	PCR	NNP
cord-009376-a35a92gh	37	12	,	,	,
cord-009376-a35a92gh	37	13	a	a	DT
cord-009376-a35a92gh	37	14	higher	high	JJR
cord-009376-a35a92gh	37	15	assay	assay	NN
cord-009376-a35a92gh	37	16	sensitivity	sensitivity	NN
cord-009376-a35a92gh	37	17	can	can	MD
cord-009376-a35a92gh	37	18	be	be	VB
cord-009376-a35a92gh	37	19	achieved	achieve	VBN
cord-009376-a35a92gh	37	20	with	with	IN
cord-009376-a35a92gh	37	21	relative	relative	JJ
cord-009376-a35a92gh	37	22	ease	ease	NN
cord-009376-a35a92gh	37	23	.	.	.
cord-009376-a35a92gh	38	1	Multiple	multiple	JJ
cord-009376-a35a92gh	38	2	replicated	replicate	VBN
cord-009376-a35a92gh	38	3	reactions	reaction	NNS
cord-009376-a35a92gh	38	4	can	can	MD
cord-009376-a35a92gh	38	5	be	be	VB
cord-009376-a35a92gh	38	6	used	use	VBN
cord-009376-a35a92gh	38	7	to	to	TO
cord-009376-a35a92gh	38	8	increase	increase	VB
cord-009376-a35a92gh	38	9	the	the	DT
cord-009376-a35a92gh	38	10	chance	chance	NN
cord-009376-a35a92gh	38	11	of	of	IN
cord-009376-a35a92gh	38	12	observing	observe	VBG
cord-009376-a35a92gh	38	13	a	a	DT
cord-009376-a35a92gh	38	14	low	low	JJ
cord-009376-a35a92gh	38	15	level	level	NN
cord-009376-a35a92gh	38	16	virus	virus	NN
cord-009376-a35a92gh	38	17	Ž	ž	NN
cord-009376-a35a92gh	38	18	below	below	IN
cord-009376-a35a92gh	38	19	the	the	DT
cord-009376-a35a92gh	38	20	limit	limit	NN
cord-009376-a35a92gh	38	21	of	of	IN
cord-009376-a35a92gh	38	22	detection	detection	NN
cord-009376-a35a92gh	38	23	Heid	Heid	NNP
cord-009376-a35a92gh	38	24	et	et	FW
cord-009376-a35a92gh	38	25	al	al	NNP
cord-009376-a35a92gh	38	26	.	.	NNP
cord-009376-a35a92gh	38	27	,	,	,
cord-009376-a35a92gh	38	28	1996	1996	CD
cord-009376-a35a92gh	38	29	;	;	:
cord-009376-a35a92gh	38	30	 	 	_SP
cord-009376-a35a92gh	38	31	.	.	.
cord-009376-a35a92gh	39	1	Livak	Livak	NNP
cord-009376-a35a92gh	39	2	and	and	CC
cord-009376-a35a92gh	39	3	Perkin	Perkin	NNP
cord-009376-a35a92gh	39	4	Elmer	Elmer	NNP
cord-009376-a35a92gh	39	5	,	,	,
cord-009376-a35a92gh	39	6	1998	1998	CD
cord-009376-a35a92gh	39	7	.	.	.
cord-009376-a35a92gh	40	1	In	in	IN
cord-009376-a35a92gh	40	2	addition	addition	NN
cord-009376-a35a92gh	40	3	,	,	,
cord-009376-a35a92gh	40	4	coupling	couple	VBG
cord-009376-a35a92gh	40	5	real	real	JJ
cord-009376-a35a92gh	40	6	-	-	HYPH
cord-009376-a35a92gh	40	7	time	time	NN
cord-009376-a35a92gh	40	8	Q	q	NN
cord-009376-a35a92gh	40	9	-	-	HYPH
cord-009376-a35a92gh	40	10	PCR	PCR	NNP
cord-009376-a35a92gh	40	11	to	to	IN
cord-009376-a35a92gh	40	12	robotic	robotic	JJ
cord-009376-a35a92gh	40	13	extraction	extraction	NN
cord-009376-a35a92gh	40	14	and	and	CC
cord-009376-a35a92gh	40	15	PCR	PCR	NNP
cord-009376-a35a92gh	40	16	set	set	VBN
cord-009376-a35a92gh	40	17	-	-	HYPH
cord-009376-a35a92gh	40	18	up	up	RP
cord-009376-a35a92gh	40	19	systems	system	NNS
cord-009376-a35a92gh	40	20	will	will	MD
cord-009376-a35a92gh	40	21	increase	increase	VB
cord-009376-a35a92gh	40	22	throughput	throughput	NN
cord-009376-a35a92gh	40	23	further	far	RBR
cord-009376-a35a92gh	40	24	and	and	CC
cord-009376-a35a92gh	40	25	minimise	minimise	VB
cord-009376-a35a92gh	40	26	human	human	JJ
cord-009376-a35a92gh	40	27	error	error	NN
cord-009376-a35a92gh	40	28	.	.	.
cord-009376-a35a92gh	41	1	C	c	NN
cord-009376-a35a92gh	41	2	values	value	NNS
cord-009376-a35a92gh	41	3	are	be	VBP
cord-009376-a35a92gh	41	4	also	also	RB
cord-009376-a35a92gh	41	5	less	less	RBR
cord-009376-a35a92gh	41	6	sensitive	sensitive	JJ
cord-009376-a35a92gh	41	7	than	than	IN
cord-009376-a35a92gh	41	8	end	end	NN
cord-009376-a35a92gh	41	9	-	-	HYPH
cord-009376-a35a92gh	41	10	point	point	NN
cord-009376-a35a92gh	41	11	T	t	NN
cord-009376-a35a92gh	41	12	reactions	reaction	NNS
cord-009376-a35a92gh	41	13	to	to	IN
cord-009376-a35a92gh	41	14	the	the	DT
cord-009376-a35a92gh	41	15	effects	effect	NNS
cord-009376-a35a92gh	41	16	of	of	IN
cord-009376-a35a92gh	41	17	inhibitors	inhibitor	NNS
cord-009376-a35a92gh	41	18	.	.	.
cord-009376-a35a92gh	42	1	Compared	compare	VBN
cord-009376-a35a92gh	42	2	to	to	IN
cord-009376-a35a92gh	42	3	endpoint	endpoint	NN
cord-009376-a35a92gh	42	4	PCR	PCR	NNP
cord-009376-a35a92gh	42	5	,	,	,
cord-009376-a35a92gh	42	6	real	real	JJ
cord-009376-a35a92gh	42	7	time	time	NN
cord-009376-a35a92gh	42	8	Q	q	NN
cord-009376-a35a92gh	42	9	-	-	HYPH
cord-009376-a35a92gh	42	10	PCR	PCR	NNP
cord-009376-a35a92gh	42	11	offers	offer	VBZ
cord-009376-a35a92gh	42	12	streamline	streamline	VBP
cord-009376-a35a92gh	42	13	assay	assay	NN
cord-009376-a35a92gh	42	14	development	development	NN
cord-009376-a35a92gh	42	15	,	,	,
cord-009376-a35a92gh	42	16	reproducible	reproducible	JJ
cord-009376-a35a92gh	42	17	results	result	NNS
cord-009376-a35a92gh	42	18	and	and	CC
cord-009376-a35a92gh	42	19	a	a	DT
cord-009376-a35a92gh	42	20	large	large	JJ
cord-009376-a35a92gh	42	21	dynamic	dynamic	JJ
cord-009376-a35a92gh	42	22	range	range	NN
cord-009376-a35a92gh	42	23	.	.	.
cord-009376-a35a92gh	43	1	More	more	RBR
cord-009376-a35a92gh	43	2	importantly	importantly	RB
cord-009376-a35a92gh	43	3	,	,	,
cord-009376-a35a92gh	43	4	if	if	IN
cord-009376-a35a92gh	43	5	the	the	DT
cord-009376-a35a92gh	43	6	sample	sample	NN
cord-009376-a35a92gh	43	7	contains	contain	VBZ
cord-009376-a35a92gh	43	8	viral	viral	JJ
cord-009376-a35a92gh	43	9	sequences	sequence	NNS
cord-009376-a35a92gh	43	10	,	,	,
cord-009376-a35a92gh	43	11	then	then	RB
cord-009376-a35a92gh	43	12	the	the	DT
cord-009376-a35a92gh	43	13	amount	amount	NN
cord-009376-a35a92gh	43	14	of	of	IN
cord-009376-a35a92gh	43	15	target	target	NN
cord-009376-a35a92gh	43	16	nucleic	nucleic	JJ
cord-009376-a35a92gh	43	17	acid	acid	NN
cord-009376-a35a92gh	43	18	molecule	molecule	NN
cord-009376-a35a92gh	43	19	present	present	NN
cord-009376-a35a92gh	43	20	is	be	VBZ
cord-009376-a35a92gh	43	21	given	give	VBN
cord-009376-a35a92gh	43	22	.	.	.
cord-009376-a35a92gh	44	1	This	this	DT
cord-009376-a35a92gh	44	2	will	will	MD
cord-009376-a35a92gh	44	3	provide	provide	VB
cord-009376-a35a92gh	44	4	an	an	DT
cord-009376-a35a92gh	44	5	indication	indication	NN
cord-009376-a35a92gh	44	6	of	of	IN
cord-009376-a35a92gh	44	7	the	the	DT
cord-009376-a35a92gh	44	8	level	level	NN
cord-009376-a35a92gh	44	9	of	of	IN
cord-009376-a35a92gh	44	10	viral	viral	JJ
cord-009376-a35a92gh	44	11	contamination	contamination	NN
cord-009376-a35a92gh	44	12	present	present	NN
cord-009376-a35a92gh	44	13	,	,	,
cord-009376-a35a92gh	44	14	which	which	WDT
cord-009376-a35a92gh	44	15	could	could	MD
cord-009376-a35a92gh	44	16	be	be	VB
cord-009376-a35a92gh	44	17	useful	useful	JJ
cord-009376-a35a92gh	44	18	in	in	IN
cord-009376-a35a92gh	44	19	deciding	decide	VBG
cord-009376-a35a92gh	44	20	what	what	WP
cord-009376-a35a92gh	44	21	further	further	JJ
cord-009376-a35a92gh	44	22	action	action	NN
cord-009376-a35a92gh	44	23	to	to	TO
cord-009376-a35a92gh	44	24	take	take	VB
cord-009376-a35a92gh	44	25	.	.	.
cord-009376-a35a92gh	45	1	If	if	IN
cord-009376-a35a92gh	45	2	,	,	,
cord-009376-a35a92gh	45	3	for	for	IN
cord-009376-a35a92gh	45	4	example	example	NN
cord-009376-a35a92gh	45	5	,	,	,
cord-009376-a35a92gh	45	6	the	the	DT
cord-009376-a35a92gh	45	7	PCR	PCR	NNP
cord-009376-a35a92gh	45	8	-	-	HYPH
cord-009376-a35a92gh	45	9	positive	positive	JJ
cord-009376-a35a92gh	45	10	sample	sample	NN
cord-009376-a35a92gh	45	11	was	be	VBD
cord-009376-a35a92gh	45	12	from	from	IN
cord-009376-a35a92gh	45	13	bulk	bulk	JJ
cord-009376-a35a92gh	45	14	harvest	harvest	NN
cord-009376-a35a92gh	45	15	material	material	NN
cord-009376-a35a92gh	45	16	,	,	,
cord-009376-a35a92gh	45	17	further	further	JJ
cord-009376-a35a92gh	45	18	process	process	NN
cord-009376-a35a92gh	45	19	validation	validation	NN
cord-009376-a35a92gh	45	20	could	could	MD
cord-009376-a35a92gh	45	21	be	be	VB
cord-009376-a35a92gh	45	22	used	use	VBN
cord-009376-a35a92gh	45	23	to	to	TO
cord-009376-a35a92gh	45	24	determine	determine	VB
cord-009376-a35a92gh	45	25	if	if	IN
cord-009376-a35a92gh	45	26	the	the	DT
cord-009376-a35a92gh	45	27	sample	sample	NN
cord-009376-a35a92gh	45	28	still	still	RB
cord-009376-a35a92gh	45	29	gave	give	VBD
cord-009376-a35a92gh	45	30	a	a	DT
cord-009376-a35a92gh	45	31	posi	posi	JJ
cord-009376-a35a92gh	45	32	-	-	HYPH
cord-009376-a35a92gh	45	33	Ž	ž	JJ
cord-009376-a35a92gh	45	34	tive	tive	JJ
cord-009376-a35a92gh	45	35	result	result	NN
cord-009376-a35a92gh	45	36	Heid	Heid	NNP
cord-009376-a35a92gh	45	37	et	et	FW
cord-009376-a35a92gh	45	38	al	al	NNP
cord-009376-a35a92gh	45	39	.	.	.
cord-009376-a35a92gh	46	1	,	,	,
cord-009376-a35a92gh	46	2	1996	1996	CD
cord-009376-a35a92gh	46	3	;	;	:
cord-009376-a35a92gh	46	4	Livak	Livak	NNP
cord-009376-a35a92gh	46	5	and	and	CC
cord-009376-a35a92gh	46	6	Perkin	Perkin	NNP
cord-009376-a35a92gh	46	7	.	.	.
cord-009376-a35a92gh	47	1	Elmer	Elmer	NNP
cord-009376-a35a92gh	47	2	,	,	,
cord-009376-a35a92gh	47	3	1998	1998	CD
cord-009376-a35a92gh	47	4	.	.	.
cord-009376-a35a92gh	48	1	Primer	primer	NN
cord-009376-a35a92gh	48	2	design	design	NN
cord-009376-a35a92gh	48	3	for	for	IN
cord-009376-a35a92gh	48	4	real	real	JJ
cord-009376-a35a92gh	48	5	-	-	HYPH
cord-009376-a35a92gh	48	6	time	time	NN
cord-009376-a35a92gh	48	7	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	48	8	PCR	PCR	NNP
cord-009376-a35a92gh	48	9	can	can	MD
cord-009376-a35a92gh	48	10	be	be	VB
cord-009376-a35a92gh	48	11	performed	perform	VBN
cord-009376-a35a92gh	48	12	using	use	VBG
cord-009376-a35a92gh	48	13	the	the	DT
cord-009376-a35a92gh	48	14	Primer	Primer	NNP
cord-009376-a35a92gh	48	15	Express	Express	NNP
cord-009376-a35a92gh	48	16	TM	TM	NNP
cord-009376-a35a92gh	48	17	Software	Software	NNP
cord-009376-a35a92gh	48	18	on	on	IN
cord-009376-a35a92gh	48	19	the	the	DT
cord-009376-a35a92gh	48	20	ABI	ABI	NNP
cord-009376-a35a92gh	48	21	5700	5700	CD
cord-009376-a35a92gh	48	22	,	,	,
cord-009376-a35a92gh	48	23	7700	7700	CD
cord-009376-a35a92gh	48	24	,	,	,
cord-009376-a35a92gh	48	25	or	or	CC
cord-009376-a35a92gh	48	26	7900HT	7900ht	CD
cord-009376-a35a92gh	48	27	following	follow	VBG
cord-009376-a35a92gh	48	28	PE	PE	NNP
cord-009376-a35a92gh	48	29	Applied	Applied	NNP
cord-009376-a35a92gh	48	30	Biosystems	Biosystems	NNP
cord-009376-a35a92gh	48	31	recommendations	recommendation	NNS
cord-009376-a35a92gh	48	32	.	.	.
cord-009376-a35a92gh	49	1	One	one	CD
cord-009376-a35a92gh	49	2	of	of	IN
cord-009376-a35a92gh	49	3	the	the	DT
cord-009376-a35a92gh	49	4	first	first	JJ
cord-009376-a35a92gh	49	5	steps	step	NNS
cord-009376-a35a92gh	49	6	in	in	IN
cord-009376-a35a92gh	49	7	the	the	DT
cord-009376-a35a92gh	49	8	identification	identification	NN
cord-009376-a35a92gh	49	9	of	of	IN
cord-009376-a35a92gh	49	10	the	the	DT
cord-009376-a35a92gh	49	11	nucleic	nucleic	JJ
cord-009376-a35a92gh	49	12	acid	acid	NN
cord-009376-a35a92gh	49	13	target	target	NN
cord-009376-a35a92gh	49	14	is	be	VBZ
cord-009376-a35a92gh	49	15	comparison	comparison	NN
cord-009376-a35a92gh	49	16	of	of	IN
cord-009376-a35a92gh	49	17	the	the	DT
cord-009376-a35a92gh	49	18	target	target	NN
cord-009376-a35a92gh	49	19	sequence	sequence	NN
cord-009376-a35a92gh	49	20	or	or	CC
cord-009376-a35a92gh	49	21	amplicon	amplicon	NN
cord-009376-a35a92gh	49	22	using	use	VBG
cord-009376-a35a92gh	49	23	multiple	multiple	JJ
cord-009376-a35a92gh	49	24	sequence	sequence	NN
cord-009376-a35a92gh	49	25	alignments	alignment	NNS
cord-009376-a35a92gh	49	26	with	with	IN
cord-009376-a35a92gh	49	27	DNA	dna	NN
cord-009376-a35a92gh	49	28	sequence	sequence	NN
cord-009376-a35a92gh	49	29	databanks	databank	NNS
cord-009376-a35a92gh	49	30	.	.	.
cord-009376-a35a92gh	50	1	This	this	DT
cord-009376-a35a92gh	50	2	allows	allow	VBZ
cord-009376-a35a92gh	50	3	an	an	DT
cord-009376-a35a92gh	50	4	assessment	assessment	NN
cord-009376-a35a92gh	50	5	of	of	IN
cord-009376-a35a92gh	50	6	potential	potential	JJ
cord-009376-a35a92gh	50	7	cross	cross	JJ
cord-009376-a35a92gh	50	8	-	-	NN
cord-009376-a35a92gh	50	9	reaction	reaction	NN
cord-009376-a35a92gh	50	10	with	with	IN
cord-009376-a35a92gh	50	11	other	other	JJ
cord-009376-a35a92gh	50	12	related	related	JJ
cord-009376-a35a92gh	50	13	sequences	sequence	NNS
cord-009376-a35a92gh	50	14	that	that	WDT
cord-009376-a35a92gh	50	15	may	may	MD
cord-009376-a35a92gh	50	16	produce	produce	VB
cord-009376-a35a92gh	50	17	problems	problem	NNS
cord-009376-a35a92gh	50	18	in	in	IN
cord-009376-a35a92gh	50	19	the	the	DT
cord-009376-a35a92gh	50	20	Q	Q	NNP
cord-009376-a35a92gh	50	21	-	-	HYPH
cord-009376-a35a92gh	50	22	PCR	PCR	NNP
cord-009376-a35a92gh	50	23	testing	testing	NN
cord-009376-a35a92gh	50	24	.	.	.
cord-009376-a35a92gh	51	1	Determination	determination	NN
cord-009376-a35a92gh	51	2	of	of	IN
cord-009376-a35a92gh	51	3	primer	primer	NN
cord-009376-a35a92gh	51	4	specificity	specificity	NN
cord-009376-a35a92gh	51	5	is	be	VBZ
cord-009376-a35a92gh	51	6	of	of	IN
cord-009376-a35a92gh	51	7	utmost	utmost	JJ
cord-009376-a35a92gh	51	8	importance	importance	NN
cord-009376-a35a92gh	51	9	when	when	WRB
cord-009376-a35a92gh	51	10	performing	perform	VBG
cord-009376-a35a92gh	51	11	pathogen	pathogen	NN
cord-009376-a35a92gh	51	12	detection	detection	NN
cord-009376-a35a92gh	51	13	studies	study	NNS
cord-009376-a35a92gh	51	14	.	.	.
cord-009376-a35a92gh	52	1	False	false	JJ
cord-009376-a35a92gh	52	2	positive	positive	JJ
cord-009376-a35a92gh	52	3	reactions	reaction	NNS
cord-009376-a35a92gh	52	4	,	,	,
cord-009376-a35a92gh	52	5	although	although	IN
cord-009376-a35a92gh	52	6	uncommon	uncommon	JJ
cord-009376-a35a92gh	52	7	,	,	,
cord-009376-a35a92gh	52	8	can	can	MD
cord-009376-a35a92gh	52	9	lead	lead	VB
cord-009376-a35a92gh	52	10	to	to	IN
cord-009376-a35a92gh	52	11	major	major	JJ
cord-009376-a35a92gh	52	12	problems	problem	NNS
cord-009376-a35a92gh	52	13	in	in	IN
cord-009376-a35a92gh	52	14	the	the	DT
cord-009376-a35a92gh	52	15	release	release	NN
cord-009376-a35a92gh	52	16	of	of	IN
cord-009376-a35a92gh	52	17	biotechnology	biotechnology	NN
cord-009376-a35a92gh	52	18	products	product	NNS
cord-009376-a35a92gh	52	19	for	for	IN
cord-009376-a35a92gh	52	20	clinical	clinical	JJ
cord-009376-a35a92gh	52	21	trial	trial	NN
cord-009376-a35a92gh	52	22	.	.	.
cord-009376-a35a92gh	53	1	Therefore	therefore	RB
cord-009376-a35a92gh	53	2	,	,	,
cord-009376-a35a92gh	53	3	assay	assay	NN
cord-009376-a35a92gh	53	4	specificity	specificity	NN
cord-009376-a35a92gh	53	5	studies	study	NNS
cord-009376-a35a92gh	53	6	are	be	VBP
cord-009376-a35a92gh	53	7	critical	critical	JJ
cord-009376-a35a92gh	53	8	during	during	IN
cord-009376-a35a92gh	53	9	validation	validation	NN
cord-009376-a35a92gh	53	10	to	to	TO
cord-009376-a35a92gh	53	11	ensure	ensure	VB
cord-009376-a35a92gh	53	12	confidence	confidence	NN
cord-009376-a35a92gh	53	13	in	in	IN
cord-009376-a35a92gh	53	14	reporting	report	VBG
cord-009376-a35a92gh	53	15	positive	positive	JJ
cord-009376-a35a92gh	53	16	results	result	NNS
cord-009376-a35a92gh	53	17	.	.	.
cord-009376-a35a92gh	54	1	The	the	DT
cord-009376-a35a92gh	54	2	Q	Q	NNP
cord-009376-a35a92gh	54	3	-	-	HYPH
cord-009376-a35a92gh	54	4	PCR	pcr	NN
cord-009376-a35a92gh	54	5	assay	assay	NN
cord-009376-a35a92gh	54	6	should	should	MD
cord-009376-a35a92gh	54	7	be	be	VB
cord-009376-a35a92gh	54	8	able	able	JJ
cord-009376-a35a92gh	54	9	to	to	TO
cord-009376-a35a92gh	54	10	discriminate	discriminate	VB
cord-009376-a35a92gh	54	11	between	between	IN
cord-009376-a35a92gh	54	12	the	the	DT
cord-009376-a35a92gh	54	13	target	target	NN
cord-009376-a35a92gh	54	14	and	and	CC
cord-009376-a35a92gh	54	15	nucleic	nucleic	JJ
cord-009376-a35a92gh	54	16	acids	acid	NNS
cord-009376-a35a92gh	54	17	that	that	WDT
cord-009376-a35a92gh	54	18	are	be	VBP
cord-009376-a35a92gh	54	19	likely	likely	JJ
cord-009376-a35a92gh	54	20	to	to	TO
cord-009376-a35a92gh	54	21	be	be	VB
cord-009376-a35a92gh	54	22	present	present	JJ
cord-009376-a35a92gh	54	23	in	in	IN
cord-009376-a35a92gh	54	24	test	test	NN
cord-009376-a35a92gh	54	25	material	material	NN
cord-009376-a35a92gh	54	26	.	.	.
cord-009376-a35a92gh	55	1	For	for	IN
cord-009376-a35a92gh	55	2	detection	detection	NN
cord-009376-a35a92gh	55	3	of	of	IN
cord-009376-a35a92gh	55	4	viruses	virus	NNS
cord-009376-a35a92gh	55	5	such	such	JJ
cord-009376-a35a92gh	55	6	as	as	IN
cord-009376-a35a92gh	55	7	Human	Human	NNP
cord-009376-a35a92gh	55	8	im	im	NNP
cord-009376-a35a92gh	55	9	-	-	HYPH
cord-009376-a35a92gh	55	10	Ž	ž	NN
cord-009376-a35a92gh	55	11	.	.	.
cord-009376-a35a92gh	56	1	munodeficiency	munodeficiency	NNP
cord-009376-a35a92gh	56	2	virus	virus	NN
cord-009376-a35a92gh	56	3	HIV	HIV	NNP
cord-009376-a35a92gh	56	4	,	,	,
cord-009376-a35a92gh	56	5	and	and	CC
cord-009376-a35a92gh	56	6	other	other	JJ
cord-009376-a35a92gh	56	7	viruses	virus	NNS
cord-009376-a35a92gh	56	8	which	which	WDT
cord-009376-a35a92gh	56	9	show	show	VBP
cord-009376-a35a92gh	56	10	sequence	sequence	NN
cord-009376-a35a92gh	56	11	variation	variation	NN
cord-009376-a35a92gh	56	12	,	,	,
cord-009376-a35a92gh	56	13	it	-PRON-	PRP
cord-009376-a35a92gh	56	14	is	be	VBZ
cord-009376-a35a92gh	56	15	extremely	extremely	RB
cord-009376-a35a92gh	56	16	important	important	JJ
cord-009376-a35a92gh	56	17	to	to	TO
cord-009376-a35a92gh	56	18	ensure	ensure	VB
cord-009376-a35a92gh	56	19	the	the	DT
cord-009376-a35a92gh	56	20	primers	primer	NNS
cord-009376-a35a92gh	56	21	and	and	CC
cord-009376-a35a92gh	56	22	probe	probe	NN
cord-009376-a35a92gh	56	23	are	be	VBP
cord-009376-a35a92gh	56	24	targeted	target	VBN
cord-009376-a35a92gh	56	25	to	to	IN
cord-009376-a35a92gh	56	26	a	a	DT
cord-009376-a35a92gh	56	27	conserved	conserved	JJ
cord-009376-a35a92gh	56	28	region	region	NN
cord-009376-a35a92gh	56	29	of	of	IN
cord-009376-a35a92gh	56	30	the	the	DT
cord-009376-a35a92gh	56	31	viral	viral	JJ
cord-009376-a35a92gh	56	32	genome	genome	NN
cord-009376-a35a92gh	56	33	.	.	.
cord-009376-a35a92gh	57	1	Selection	selection	NN
cord-009376-a35a92gh	57	2	of	of	IN
cord-009376-a35a92gh	57	3	a	a	DT
cord-009376-a35a92gh	57	4	region	region	NN
cord-009376-a35a92gh	57	5	with	with	IN
cord-009376-a35a92gh	57	6	highly	highly	RB
cord-009376-a35a92gh	57	7	conserved	conserve	VBN
cord-009376-a35a92gh	57	8	sequences	sequence	NNS
cord-009376-a35a92gh	57	9	facilitates	facilitate	VBZ
cord-009376-a35a92gh	57	10	the	the	DT
cord-009376-a35a92gh	57	11	design	design	NN
cord-009376-a35a92gh	57	12	of	of	IN
cord-009376-a35a92gh	57	13	degenerate	degenerate	JJ
cord-009376-a35a92gh	57	14	amplimers	amplimer	NNS
cord-009376-a35a92gh	57	15	and	and	CC
cord-009376-a35a92gh	57	16	probes	probe	NNS
cord-009376-a35a92gh	57	17	,	,	,
cord-009376-a35a92gh	57	18	allowing	allow	VBG
cord-009376-a35a92gh	57	19	detection	detection	NN
cord-009376-a35a92gh	57	20	of	of	IN
cord-009376-a35a92gh	57	21	the	the	DT
cord-009376-a35a92gh	57	22	maximum	maximum	JJ
cord-009376-a35a92gh	57	23	amount	amount	NN
cord-009376-a35a92gh	57	24	of	of	IN
cord-009376-a35a92gh	57	25	variants	variant	NNS
cord-009376-a35a92gh	57	26	and	and	CC
cord-009376-a35a92gh	57	27	strains	strain	NNS
cord-009376-a35a92gh	57	28	possible	possible	JJ
cord-009376-a35a92gh	57	29	Ž	ž	NN
cord-009376-a35a92gh	57	30	.	.	.
cord-009376-a35a92gh	58	1	Shown	show	VBN
cord-009376-a35a92gh	58	2	in	in	IN
cord-009376-a35a92gh	58	3	Fig	Fig	NNP
cord-009376-a35a92gh	58	4	.	.	.
cord-009376-a35a92gh	59	1	3	3	CD
cord-009376-a35a92gh	59	2	for	for	IN
cord-009376-a35a92gh	59	3	BovinerPorcine	BovinerPorcine	NNP
cord-009376-a35a92gh	59	4	circovirus	circovirus	NNP
cord-009376-a35a92gh	59	5	.	.	.
cord-009376-a35a92gh	60	1	In	in	IN
cord-009376-a35a92gh	60	2	addition	addition	NN
cord-009376-a35a92gh	60	3	,	,	,
cord-009376-a35a92gh	60	4	the	the	DT
cord-009376-a35a92gh	60	5	stability	stability	NN
cord-009376-a35a92gh	60	6	of	of	IN
cord-009376-a35a92gh	60	7	the	the	DT
cord-009376-a35a92gh	60	8	target	target	NN
cord-009376-a35a92gh	60	9	sequence	sequence	NN
cord-009376-a35a92gh	60	10	should	should	MD
cord-009376-a35a92gh	60	11	be	be	VB
cord-009376-a35a92gh	60	12	considered	consider	VBN
cord-009376-a35a92gh	60	13	,	,	,
cord-009376-a35a92gh	60	14	in	in	IN
cord-009376-a35a92gh	60	15	an	an	DT
cord-009376-a35a92gh	60	16	attempt	attempt	NN
cord-009376-a35a92gh	60	17	to	to	TO
cord-009376-a35a92gh	60	18	ensure	ensure	VB
cord-009376-a35a92gh	60	19	that	that	IN
cord-009376-a35a92gh	60	20	the	the	DT
cord-009376-a35a92gh	60	21	amplicon	amplicon	NN
cord-009376-a35a92gh	60	22	does	do	VBZ
cord-009376-a35a92gh	60	23	not	not	RB
cord-009376-a35a92gh	60	24	reside	reside	VB
cord-009376-a35a92gh	60	25	on	on	IN
cord-009376-a35a92gh	60	26	an	an	DT
cord-009376-a35a92gh	60	27	unstable	unstable	JJ
cord-009376-a35a92gh	60	28	genetic	genetic	JJ
cord-009376-a35a92gh	60	29	element	element	NN
cord-009376-a35a92gh	60	30	that	that	WDT
cord-009376-a35a92gh	60	31	may	may	MD
cord-009376-a35a92gh	60	32	be	be	VB
cord-009376-a35a92gh	60	33	a	a	DT
cord-009376-a35a92gh	60	34	hot	hot	JJ
cord-009376-a35a92gh	60	35	spot	spot	NN
cord-009376-a35a92gh	60	36	for	for	IN
cord-009376-a35a92gh	60	37	deletions	deletion	NNS
cord-009376-a35a92gh	60	38	,	,	,
cord-009376-a35a92gh	60	39	such	such	JJ
cord-009376-a35a92gh	60	40	as	as	IN
cord-009376-a35a92gh	60	41	those	those	DT
cord-009376-a35a92gh	60	42	that	that	WDT
cord-009376-a35a92gh	60	43	occur	occur	VBP
cord-009376-a35a92gh	60	44	in	in	IN
cord-009376-a35a92gh	60	45	the	the	DT
cord-009376-a35a92gh	60	46	Polyoma	Polyoma	NNP
cord-009376-a35a92gh	60	47	Ž	Ž	NNP
cord-009376-a35a92gh	60	48	.	.	.
cord-009376-a35a92gh	61	1	virus	virus	NN
cord-009376-a35a92gh	61	2	Bendig	Bendig	NNP
cord-009376-a35a92gh	61	3	and	and	CC
cord-009376-a35a92gh	61	4	Folk	Folk	NNP
cord-009376-a35a92gh	61	5	,	,	,
cord-009376-a35a92gh	61	6	1979	1979	CD
cord-009376-a35a92gh	61	7	;	;	:
cord-009376-a35a92gh	61	8	Persing	Persing	NNP
cord-009376-a35a92gh	61	9	,	,	,
cord-009376-a35a92gh	61	10	1993	1993	CD
cord-009376-a35a92gh	61	11	.	.	.
cord-009376-a35a92gh	62	1	To	to	TO
cord-009376-a35a92gh	62	2	ensure	ensure	VB
cord-009376-a35a92gh	62	3	technical	technical	JJ
cord-009376-a35a92gh	62	4	reliability	reliability	NN
cord-009376-a35a92gh	62	5	,	,	,
cord-009376-a35a92gh	62	6	assay	assay	NN
cord-009376-a35a92gh	62	7	optimisa	optimisa	NNP
cord-009376-a35a92gh	62	8	-	-	HYPH
cord-009376-a35a92gh	62	9	tion	tion	NN
cord-009376-a35a92gh	62	10	studies	study	NNS
cord-009376-a35a92gh	62	11	are	be	VBP
cord-009376-a35a92gh	62	12	essential	essential	JJ
cord-009376-a35a92gh	62	13	,	,	,
cord-009376-a35a92gh	62	14	together	together	RB
cord-009376-a35a92gh	62	15	with	with	IN
cord-009376-a35a92gh	62	16	validation	validation	NN
cord-009376-a35a92gh	62	17	Ž	ž	NN
cord-009376-a35a92gh	62	18	to	to	IN
cord-009376-a35a92gh	62	19	ICH	ICH	NNP
cord-009376-a35a92gh	62	20	guidelines	guideline	NNS
cord-009376-a35a92gh	63	1	ICH	ICH	NNP
cord-009376-a35a92gh	63	2	Topic	Topic	NNP
cord-009376-a35a92gh	63	3	Q2A	Q2A	NNP
cord-009376-a35a92gh	63	4	,	,	,
cord-009376-a35a92gh	63	5	1995	1995	CD
cord-009376-a35a92gh	63	6	;	;	:
cord-009376-a35a92gh	63	7	ICH	ICH	NNP
cord-009376-a35a92gh	63	8	.	.	.
cord-009376-a35a92gh	64	1	Topic	Topic	NNP
cord-009376-a35a92gh	64	2	Q2B	Q2B	NNP
cord-009376-a35a92gh	64	3	,	,	,
cord-009376-a35a92gh	64	4	1997	1997	CD
cord-009376-a35a92gh	64	5	;	;	.
cord-009376-a35a92gh	65	1	Cope	cope	VB
cord-009376-a35a92gh	65	2	,	,	,
cord-009376-a35a92gh	65	3	2000	2000	CD
cord-009376-a35a92gh	65	4	.	.	.
cord-009376-a35a92gh	66	1	A	a	DT
cord-009376-a35a92gh	66	2	minimum	minimum	NN
cord-009376-a35a92gh	66	3	of	of	IN
cord-009376-a35a92gh	66	4	two	two	CD
cord-009376-a35a92gh	66	5	operators	operator	NNS
cord-009376-a35a92gh	66	6	should	should	MD
cord-009376-a35a92gh	66	7	perform	perform	VB
cord-009376-a35a92gh	66	8	specificity	specificity	NN
cord-009376-a35a92gh	66	9	,	,	,
cord-009376-a35a92gh	66	10	linearity	linearity	NN
cord-009376-a35a92gh	66	11	,	,	,
cord-009376-a35a92gh	66	12	detection	detection	NN
cord-009376-a35a92gh	66	13	limit	limit	NN
cord-009376-a35a92gh	66	14	,	,	,
cord-009376-a35a92gh	66	15	accuracy	accuracy	NN
cord-009376-a35a92gh	66	16	and	and	CC
cord-009376-a35a92gh	66	17	repeatability	repeatability	NN
cord-009376-a35a92gh	66	18	studies	study	NNS
cord-009376-a35a92gh	66	19	.	.	.
cord-009376-a35a92gh	67	1	Each	each	DT
cord-009376-a35a92gh	67	2	operator	operator	NN
cord-009376-a35a92gh	67	3	should	should	MD
cord-009376-a35a92gh	67	4	determine	determine	VB
cord-009376-a35a92gh	67	5	these	these	DT
cord-009376-a35a92gh	67	6	assay	assay	NN
cord-009376-a35a92gh	67	7	parameters	parameter	NNS
cord-009376-a35a92gh	67	8	at	at	IN
cord-009376-a35a92gh	67	9	least	least	JJS
cord-009376-a35a92gh	67	10	once	once	RB
cord-009376-a35a92gh	67	11	,	,	,
cord-009376-a35a92gh	67	12	using	use	VBG
cord-009376-a35a92gh	67	13	a	a	DT
cord-009376-a35a92gh	67	14	minimum	minimum	NN
cord-009376-a35a92gh	67	15	of	of	IN
cord-009376-a35a92gh	67	16	five	five	CD
cord-009376-a35a92gh	67	17	concentrations	concentration	NNS
cord-009376-a35a92gh	67	18	over	over	IN
cord-009376-a35a92gh	67	19	the	the	DT
cord-009376-a35a92gh	67	20	range	range	NN
cord-009376-a35a92gh	67	21	of	of	IN
cord-009376-a35a92gh	67	22	the	the	DT
cord-009376-a35a92gh	67	23	assay	assay	NN
cord-009376-a35a92gh	67	24	.	.	.
cord-009376-a35a92gh	68	1	The	the	DT
cord-009376-a35a92gh	68	2	importance	importance	NN
cord-009376-a35a92gh	68	3	of	of	IN
cord-009376-a35a92gh	68	4	optimisation	optimisation	NN
cord-009376-a35a92gh	68	5	and	and	CC
cord-009376-a35a92gh	68	6	validation	validation	NN
cord-009376-a35a92gh	68	7	is	be	VBZ
cord-009376-a35a92gh	68	8	highlighted	highlight	VBN
cord-009376-a35a92gh	68	9	when	when	WRB
cord-009376-a35a92gh	68	10	working	work	VBG
cord-009376-a35a92gh	68	11	with	with	IN
cord-009376-a35a92gh	68	12	non	non	JJ
cord-009376-a35a92gh	68	13	-	-	JJ
cord-009376-a35a92gh	68	14	validated	validated	JJ
cord-009376-a35a92gh	68	15	rapidly	rapidly	RB
cord-009376-a35a92gh	68	16	developed	develop	VBN
cord-009376-a35a92gh	68	17	Q	Q	NNP
cord-009376-a35a92gh	68	18	-	-	HYPH
cord-009376-a35a92gh	68	19	PCR	PCR	NNP
cord-009376-a35a92gh	68	20	assays	assay	NNS
cord-009376-a35a92gh	68	21	.	.	.
cord-009376-a35a92gh	69	1	High	high	JJ
cord-009376-a35a92gh	69	2	background	background	NN
cord-009376-a35a92gh	69	3	fluorescent	fluorescent	NN
cord-009376-a35a92gh	69	4	signals	signal	NNS
cord-009376-a35a92gh	69	5	leading	lead	VBG
cord-009376-a35a92gh	69	6	to	to	IN
cord-009376-a35a92gh	69	7	reduced	reduce	VBN
cord-009376-a35a92gh	69	8	assay	assay	NN
cord-009376-a35a92gh	69	9	sensitivity	sensitivity	NN
cord-009376-a35a92gh	69	10	and	and	CC
cord-009376-a35a92gh	69	11	abnormal	abnormal	JJ
cord-009376-a35a92gh	69	12	amplification	amplification	NN
cord-009376-a35a92gh	69	13	signals	signal	NNS
cord-009376-a35a92gh	69	14	have	have	VBP
cord-009376-a35a92gh	69	15	been	be	VBN
cord-009376-a35a92gh	69	16	observed	observe	VBN
cord-009376-a35a92gh	69	17	in	in	IN
cord-009376-a35a92gh	69	18	assays	assay	NNS
cord-009376-a35a92gh	69	19	before	before	IN
cord-009376-a35a92gh	69	20	assay	assay	NN
cord-009376-a35a92gh	69	21	optimisation	optimisation	NN
cord-009376-a35a92gh	69	22	and	and	CC
cord-009376-a35a92gh	69	23	validation	validation	NN
cord-009376-a35a92gh	69	24	,	,	,
cord-009376-a35a92gh	69	25	further	further	RB
cord-009376-a35a92gh	69	26	supporting	support	VBG
cord-009376-a35a92gh	69	27	the	the	DT
cord-009376-a35a92gh	69	28	need	need	NN
cord-009376-a35a92gh	69	29	for	for	IN
cord-009376-a35a92gh	69	30	assay	assay	NN
cord-009376-a35a92gh	69	31	validation	validation	NN
cord-009376-a35a92gh	69	32	.	.	.
cord-009376-a35a92gh	70	1	More	more	RBR
cord-009376-a35a92gh	70	2	importantly	importantly	RB
cord-009376-a35a92gh	70	3	,	,	,
cord-009376-a35a92gh	70	4	a	a	DT
cord-009376-a35a92gh	70	5	less	less	RBR
cord-009376-a35a92gh	70	6	experienced	experienced	JJ
cord-009376-a35a92gh	70	7	operator	operator	NN
cord-009376-a35a92gh	70	8	may	may	MD
cord-009376-a35a92gh	70	9	not	not	RB
cord-009376-a35a92gh	70	10	be	be	VB
cord-009376-a35a92gh	70	11	able	able	JJ
cord-009376-a35a92gh	70	12	to	to	TO
cord-009376-a35a92gh	70	13	distinguish	distinguish	VB
cord-009376-a35a92gh	70	14	between	between	IN
cord-009376-a35a92gh	70	15	genuine	genuine	JJ
cord-009376-a35a92gh	70	16	and	and	CC
cord-009376-a35a92gh	70	17	abnormal	abnormal	JJ
cord-009376-a35a92gh	70	18	amplification	amplification	NN
cord-009376-a35a92gh	70	19	signals	signal	NNS
cord-009376-a35a92gh	70	20	during	during	IN
cord-009376-a35a92gh	70	21	testing	testing	NN
cord-009376-a35a92gh	70	22	with	with	IN
cord-009376-a35a92gh	70	23	a	a	DT
cord-009376-a35a92gh	70	24	non	non	JJ
cord-009376-a35a92gh	70	25	-	-	JJ
cord-009376-a35a92gh	70	26	optimised	optimised	JJ
cord-009376-a35a92gh	70	27	,	,	,
cord-009376-a35a92gh	70	28	non	non	JJ
cord-009376-a35a92gh	70	29	-	-	JJ
cord-009376-a35a92gh	70	30	validated	validated	JJ
cord-009376-a35a92gh	70	31	assay	assay	NN
cord-009376-a35a92gh	70	32	.	.	.
cord-009376-a35a92gh	71	1	Primer	primer	NN
cord-009376-a35a92gh	71	2	optimisation	optimisation	NN
cord-009376-a35a92gh	71	3	is	be	VBZ
cord-009376-a35a92gh	71	4	carried	carry	VBN
cord-009376-a35a92gh	71	5	out	out	RP
cord-009376-a35a92gh	71	6	using	use	VBG
cord-009376-a35a92gh	71	7	an	an	DT
cord-009376-a35a92gh	71	8	array	array	NN
cord-009376-a35a92gh	71	9	of	of	IN
cord-009376-a35a92gh	71	10	primer	primer	NN
cord-009376-a35a92gh	71	11	concentrations	concentration	NNS
cord-009376-a35a92gh	71	12	with	with	IN
cord-009376-a35a92gh	71	13	a	a	DT
cord-009376-a35a92gh	71	14	constant	constant	JJ
cord-009376-a35a92gh	71	15	amount	amount	NN
cord-009376-a35a92gh	71	16	of	of	IN
cord-009376-a35a92gh	71	17	probe	probe	NN
cord-009376-a35a92gh	71	18	and	and	CC
cord-009376-a35a92gh	71	19	target	target	NN
cord-009376-a35a92gh	71	20	template	template	NN
cord-009376-a35a92gh	71	21	.	.	.
cord-009376-a35a92gh	72	1	Because	because	IN
cord-009376-a35a92gh	72	2	high	high	JJ
cord-009376-a35a92gh	72	3	⌬	⌬	NNS
cord-009376-a35a92gh	72	4	R	r	NN
cord-009376-a35a92gh	72	5	values	value	NNS
cord-009376-a35a92gh	72	6	reflect	reflect	VBP
cord-009376-a35a92gh	72	7	increased	increase	VBN
cord-009376-a35a92gh	72	8	levels	level	NNS
cord-009376-a35a92gh	72	9	of	of	IN
cord-009376-a35a92gh	72	10	amplin	amplin	NNP
cord-009376-a35a92gh	72	11	fication	fication	NN
cord-009376-a35a92gh	72	12	product	product	NN
cord-009376-a35a92gh	72	13	and	and	CC
cord-009376-a35a92gh	72	14	efficient	efficient	JJ
cord-009376-a35a92gh	72	15	reactions	reaction	NNS
cord-009376-a35a92gh	72	16	,	,	,
cord-009376-a35a92gh	72	17	the	the	DT
cord-009376-a35a92gh	72	18	combination	combination	NN
cord-009376-a35a92gh	72	19	of	of	IN
cord-009376-a35a92gh	72	20	forward	forward	NN
cord-009376-a35a92gh	72	21	and	and	CC
cord-009376-a35a92gh	72	22	reverse	reverse	VB
cord-009376-a35a92gh	72	23	primer	primer	NN
cord-009376-a35a92gh	72	24	showing	show	VBG
cord-009376-a35a92gh	72	25	the	the	DT
cord-009376-a35a92gh	72	26	maximum	maximum	JJ
cord-009376-a35a92gh	72	27	⌬	⌬	NNPS
cord-009376-a35a92gh	72	28	R	r	NN
cord-009376-a35a92gh	72	29	,	,	,
cord-009376-a35a92gh	72	30	is	be	VBZ
cord-009376-a35a92gh	72	31	selected	select	VBN
cord-009376-a35a92gh	72	32	.	.	.
cord-009376-a35a92gh	73	1	Probe	Probe	NNP
cord-009376-a35a92gh	73	2	optimisan	optimisan	NNP
cord-009376-a35a92gh	73	3	tion	tion	NN
cord-009376-a35a92gh	73	4	is	be	VBZ
cord-009376-a35a92gh	73	5	performed	perform	VBN
cord-009376-a35a92gh	73	6	using	use	VBG
cord-009376-a35a92gh	73	7	the	the	DT
cord-009376-a35a92gh	73	8	optimised	optimise	VBN
cord-009376-a35a92gh	73	9	primer	primer	NN
cord-009376-a35a92gh	73	10	con-	con-	NN
cord-009376-a35a92gh	73	11	ditions	dition	NNS
cord-009376-a35a92gh	73	12	,	,	,
cord-009376-a35a92gh	73	13	whereby	whereby	WRB
cord-009376-a35a92gh	73	14	the	the	DT
cord-009376-a35a92gh	73	15	concentration	concentration	NN
cord-009376-a35a92gh	73	16	of	of	IN
cord-009376-a35a92gh	73	17	probe	probe	NN
cord-009376-a35a92gh	73	18	is	be	VBZ
cord-009376-a35a92gh	73	19	varied	varied	JJ
cord-009376-a35a92gh	73	20	.	.	.
cord-009376-a35a92gh	74	1	The	the	DT
cord-009376-a35a92gh	74	2	probe	probe	NN
cord-009376-a35a92gh	74	3	concentration	concentration	NN
cord-009376-a35a92gh	74	4	showing	show	VBG
cord-009376-a35a92gh	74	5	the	the	DT
cord-009376-a35a92gh	74	6	lowest	low	JJS
cord-009376-a35a92gh	74	7	C	c	NN
cord-009376-a35a92gh	74	8	value	value	NN
cord-009376-a35a92gh	74	9	is	be	VBZ
cord-009376-a35a92gh	74	10	chosen	choose	VBN
cord-009376-a35a92gh	74	11	as	as	IN
cord-009376-a35a92gh	74	12	optimal	optimal	JJ
cord-009376-a35a92gh	74	13	,	,	,
cord-009376-a35a92gh	74	14	and	and	CC
cord-009376-a35a92gh	74	15	reflects	reflect	VBZ
cord-009376-a35a92gh	74	16	T	T	NNP
cord-009376-a35a92gh	74	17	the	the	DT
cord-009376-a35a92gh	74	18	most	most	RBS
cord-009376-a35a92gh	74	19	efficient	efficient	JJ
cord-009376-a35a92gh	74	20	reaction	reaction	NN
cord-009376-a35a92gh	74	21	conditions	condition	NNS
cord-009376-a35a92gh	74	22	.	.	.
cord-009376-a35a92gh	75	1	Assay	assay	NN
cord-009376-a35a92gh	75	2	linearity	linearity	NN
cord-009376-a35a92gh	75	3	,	,	,
cord-009376-a35a92gh	75	4	detection	detection	NN
cord-009376-a35a92gh	75	5	limit	limit	NN
cord-009376-a35a92gh	75	6	and	and	CC
cord-009376-a35a92gh	75	7	precision	precision	NN
cord-009376-a35a92gh	75	8	are	be	VBP
cord-009376-a35a92gh	75	9	assessed	assess	VBN
cord-009376-a35a92gh	75	10	using	use	VBG
cord-009376-a35a92gh	75	11	eight	eight	CD
cord-009376-a35a92gh	75	12	replicated	replicate	VBN
cord-009376-a35a92gh	75	13	reactions	reaction	NNS
cord-009376-a35a92gh	75	14	over	over	IN
cord-009376-a35a92gh	75	15	a	a	DT
cord-009376-a35a92gh	75	16	range	range	NN
cord-009376-a35a92gh	75	17	of	of	IN
cord-009376-a35a92gh	75	18	five	five	CD
cord-009376-a35a92gh	75	19	concentrations	concentration	NNS
cord-009376-a35a92gh	75	20	.	.	.
cord-009376-a35a92gh	76	1	To	to	TO
cord-009376-a35a92gh	76	2	assess	assess	VB
cord-009376-a35a92gh	76	3	repro	repro	JJ
cord-009376-a35a92gh	76	4	-	-	HYPH
cord-009376-a35a92gh	76	5	Ž	ž	NN
cord-009376-a35a92gh	76	6	2	2	CD
cord-009376-a35a92gh	76	7	.	.	.
cord-009376-a35a92gh	77	1	ducibility	ducibility	NNP
cord-009376-a35a92gh	77	2	,	,	,
cord-009376-a35a92gh	77	3	the	the	DT
cord-009376-a35a92gh	77	4	correlation	correlation	NN
cord-009376-a35a92gh	77	5	coefficient	coefficient	NN
cord-009376-a35a92gh	77	6	r	r	NN
cord-009376-a35a92gh	77	7	,	,	,
cord-009376-a35a92gh	77	8	y	y	NNP
cord-009376-a35a92gh	77	9	-	-	HYPH
cord-009376-a35a92gh	77	10	inter	inter	NNP
cord-009376-a35a92gh	77	11	-	-	JJ
cord-009376-a35a92gh	77	12	cept	cept	JJ
cord-009376-a35a92gh	77	13	and	and	CC
cord-009376-a35a92gh	77	14	slope	slope	NN
cord-009376-a35a92gh	77	15	of	of	IN
cord-009376-a35a92gh	77	16	the	the	DT
cord-009376-a35a92gh	77	17	regression	regression	NN
cord-009376-a35a92gh	77	18	line	line	NN
cord-009376-a35a92gh	77	19	should	should	MD
cord-009376-a35a92gh	77	20	be	be	VB
cord-009376-a35a92gh	77	21	calculated	calculate	VBN
cord-009376-a35a92gh	77	22	using	use	VBG
cord-009376-a35a92gh	77	23	a	a	DT
cord-009376-a35a92gh	77	24	minimum	minimum	NN
cord-009376-a35a92gh	77	25	of	of	IN
cord-009376-a35a92gh	77	26	two	two	CD
cord-009376-a35a92gh	77	27	independent	independent	JJ
cord-009376-a35a92gh	77	28	operators	operator	NNS
cord-009376-a35a92gh	77	29	on	on	IN
cord-009376-a35a92gh	77	30	the	the	DT
cord-009376-a35a92gh	77	31	ABI	ABI	NNP
cord-009376-a35a92gh	77	32	7700	7700	CD
cord-009376-a35a92gh	77	33	,	,	,
cord-009376-a35a92gh	77	34	7900HT	7900ht	CD
cord-009376-a35a92gh	77	35	,	,	,
cord-009376-a35a92gh	77	36	or	or	CC
cord-009376-a35a92gh	77	37	5700	5700	CD
cord-009376-a35a92gh	77	38	systems	system	NNS
cord-009376-a35a92gh	77	39	.	.	.
cord-009376-a35a92gh	78	1	The	the	DT
cord-009376-a35a92gh	78	2	minimum	minimum	NN
cord-009376-a35a92gh	78	3	accepted	accept	VBN
cord-009376-a35a92gh	78	4	value	value	NN
cord-009376-a35a92gh	78	5	for	for	IN
cord-009376-a35a92gh	78	6	the	the	DT
cord-009376-a35a92gh	78	7	linear	linear	JJ
cord-009376-a35a92gh	78	8	correlation	correlation	NN
cord-009376-a35a92gh	78	9	coefficient	coefficient	NN
cord-009376-a35a92gh	78	10	at	at	IN
cord-009376-a35a92gh	78	11	the	the	DT
cord-009376-a35a92gh	78	12	Ps	Ps	NNP
cord-009376-a35a92gh	78	13	0.05	0.05	CD
cord-009376-a35a92gh	78	14	statistical	statistical	JJ
cord-009376-a35a92gh	78	15	level	level	NN
cord-009376-a35a92gh	78	16	when	when	WRB
cord-009376-a35a92gh	78	17	using	use	VBG
cord-009376-a35a92gh	78	18	four	four	CD
cord-009376-a35a92gh	78	19	standards	standard	NNS
cord-009376-a35a92gh	78	20	is	be	VBZ
cord-009376-a35a92gh	78	21	0.88	0.88	CD
cord-009376-a35a92gh	78	22	Ž	ž	NN
cord-009376-a35a92gh	78	23	.	.	.
cord-009376-a35a92gh	79	1	Wardlaw	Wardlaw	NNP
cord-009376-a35a92gh	79	2	,	,	,
cord-009376-a35a92gh	79	3	1987	1987	CD
cord-009376-a35a92gh	79	4	,	,	,
cord-009376-a35a92gh	79	5	and	and	CC
cord-009376-a35a92gh	79	6	Q	Q	NNP
cord-009376-a35a92gh	79	7	-	-	HYPH
cord-009376-a35a92gh	79	8	PCR	PCR	NNP
cord-009376-a35a92gh	79	9	assays	assay	NNS
cord-009376-a35a92gh	79	10	will	will	MD
cord-009376-a35a92gh	79	11	normally	normally	RB
cord-009376-a35a92gh	79	12	show	show	VB
cord-009376-a35a92gh	79	13	values	value	NNS
cord-009376-a35a92gh	79	14	of	of	IN
cord-009376-a35a92gh	79	15	r	r	NN
cord-009376-a35a92gh	79	16	2	2	CD
cord-009376-a35a92gh	79	17	)	)	-RRB-
cord-009376-a35a92gh	79	18	0.90	0.90	CD
cord-009376-a35a92gh	79	19	.	.	.
cord-009376-a35a92gh	80	1	Two	two	CD
cord-009376-a35a92gh	80	2	major	major	JJ
cord-009376-a35a92gh	80	3	complicating	complicate	VBG
cord-009376-a35a92gh	80	4	factors	factor	NNS
cord-009376-a35a92gh	80	5	in	in	IN
cord-009376-a35a92gh	80	6	establishing	establish	VBG
cord-009376-a35a92gh	80	7	the	the	DT
cord-009376-a35a92gh	80	8	detection	detection	NN
cord-009376-a35a92gh	80	9	limit	limit	NN
cord-009376-a35a92gh	80	10	for	for	IN
cord-009376-a35a92gh	80	11	a	a	DT
cord-009376-a35a92gh	80	12	real	real	JJ
cord-009376-a35a92gh	80	13	-	-	HYPH
cord-009376-a35a92gh	80	14	time	time	NN
cord-009376-a35a92gh	80	15	PCR	pcr	NN
cord-009376-a35a92gh	80	16	assay	assay	NN
cord-009376-a35a92gh	80	17	Ž	Ž	NNP
cord-009376-a35a92gh	80	18	.	.	.
cord-009376-a35a92gh	81	1	using	use	VBG
cord-009376-a35a92gh	81	2	biological	biological	JJ
cord-009376-a35a92gh	81	3	material	material	NN
cord-009376-a35a92gh	81	4	are	be	VBP
cord-009376-a35a92gh	81	5	:	:	:
cord-009376-a35a92gh	81	6	1	1	CD
cord-009376-a35a92gh	82	1	the	the	DT
cord-009376-a35a92gh	82	2	number	number	NN
cord-009376-a35a92gh	82	3	of	of	IN
cord-009376-a35a92gh	82	4	target	target	NN
cord-009376-a35a92gh	82	5	nucleic	nucleic	JJ
cord-009376-a35a92gh	82	6	molecules	molecule	NNS
cord-009376-a35a92gh	82	7	present	present	JJ
cord-009376-a35a92gh	82	8	in	in	IN
cord-009376-a35a92gh	82	9	infected	infected	JJ
cord-009376-a35a92gh	82	10	cells	cell	NNS
cord-009376-a35a92gh	82	11	(	(	-LRB-
cord-009376-a35a92gh	82	12	)	)	-RRB-
cord-009376-a35a92gh	82	13	and	and	CC
cord-009376-a35a92gh	82	14	clinical	clinical	JJ
cord-009376-a35a92gh	82	15	samples	sample	NNS
cord-009376-a35a92gh	82	16	,	,	,
cord-009376-a35a92gh	82	17	or	or	CC
cord-009376-a35a92gh	82	18	electron	electron	NN
cord-009376-a35a92gh	82	19	microscopy	microscopy	NN
cord-009376-a35a92gh	82	20	Ž	ž	NN
cord-009376-a35a92gh	82	21	.	.	.
cord-009376-a35a92gh	83	1	counted	count	VBN
cord-009376-a35a92gh	83	2	viral	viral	JJ
cord-009376-a35a92gh	83	3	stocks	stock	NNS
cord-009376-a35a92gh	83	4	,	,	,
cord-009376-a35a92gh	83	5	may	may	MD
cord-009376-a35a92gh	83	6	be	be	VB
cord-009376-a35a92gh	83	7	variable	variable	JJ
cord-009376-a35a92gh	83	8	;	;	:
cord-009376-a35a92gh	83	9	2	2	LS
cord-009376-a35a92gh	83	10	there	there	EX
cord-009376-a35a92gh	83	11	may	may	MD
cord-009376-a35a92gh	83	12	be	be	VB
cord-009376-a35a92gh	83	13	no	no	DT
cord-009376-a35a92gh	83	14	culture	culture	NN
cord-009376-a35a92gh	83	15	methods	method	NNS
cord-009376-a35a92gh	83	16	available	available	JJ
cord-009376-a35a92gh	83	17	for	for	IN
cord-009376-a35a92gh	83	18	the	the	DT
cord-009376-a35a92gh	83	19	target	target	NN
cord-009376-a35a92gh	83	20	organism	organism	NN
cord-009376-a35a92gh	83	21	.	.	.
cord-009376-a35a92gh	84	1	Therefore	therefore	RB
cord-009376-a35a92gh	84	2	,	,	,
cord-009376-a35a92gh	84	3	the	the	DT
cord-009376-a35a92gh	84	4	limit	limit	NN
cord-009376-a35a92gh	84	5	of	of	IN
cord-009376-a35a92gh	84	6	detection	detection	NN
cord-009376-a35a92gh	84	7	is	be	VBZ
cord-009376-a35a92gh	84	8	firstly	firstly	RB
cord-009376-a35a92gh	84	9	validated	validate	VBN
cord-009376-a35a92gh	84	10	using	use	VBG
cord-009376-a35a92gh	84	11	purified	purify	VBN
cord-009376-a35a92gh	84	12	target	target	NN
cord-009376-a35a92gh	84	13	molecules	molecule	NNS
cord-009376-a35a92gh	84	14	in	in	IN
cord-009376-a35a92gh	84	15	the	the	DT
cord-009376-a35a92gh	84	16	form	form	NN
cord-009376-a35a92gh	84	17	of	of	IN
cord-009376-a35a92gh	84	18	recombinant	recombinant	JJ
cord-009376-a35a92gh	84	19	plasmid	plasmid	NN
cord-009376-a35a92gh	84	20	DNA	dna	NN
cord-009376-a35a92gh	84	21	or	or	CC
cord-009376-a35a92gh	84	22	recombinant	recombinant	JJ
cord-009376-a35a92gh	84	23	RNA	RNA	NNP
cord-009376-a35a92gh	84	24	.	.	.
cord-009376-a35a92gh	85	1	The	the	DT
cord-009376-a35a92gh	85	2	assay	assay	NN
cord-009376-a35a92gh	85	3	limit	limit	NN
cord-009376-a35a92gh	85	4	of	of	IN
cord-009376-a35a92gh	85	5	detection	detection	NN
cord-009376-a35a92gh	85	6	with	with	IN
cord-009376-a35a92gh	85	7	95	95	CD
cord-009376-a35a92gh	85	8	%	%	NN
cord-009376-a35a92gh	85	9	confidence	confidence	NN
cord-009376-a35a92gh	85	10	,	,	,
cord-009376-a35a92gh	85	11	is	be	VBZ
cord-009376-a35a92gh	85	12	defined	define	VBN
cord-009376-a35a92gh	85	13	as	as	IN
cord-009376-a35a92gh	85	14	the	the	DT
cord-009376-a35a92gh	85	15	lowest	low	JJS
cord-009376-a35a92gh	85	16	amount	amount	NN
cord-009376-a35a92gh	85	17	of	of	IN
cord-009376-a35a92gh	85	18	target	target	NN
cord-009376-a35a92gh	85	19	producing	produce	VBG
cord-009376-a35a92gh	85	20	a	a	DT
cord-009376-a35a92gh	85	21	confidence	confidence	NN
cord-009376-a35a92gh	85	22	interval	interval	NN
cord-009376-a35a92gh	85	23	that	that	WDT
cord-009376-a35a92gh	85	24	is	be	VBZ
cord-009376-a35a92gh	85	25	significantly	significantly	RB
cord-009376-a35a92gh	85	26	lower	low	JJR
cord-009376-a35a92gh	85	27	than	than	IN
cord-009376-a35a92gh	85	28	that	that	DT
cord-009376-a35a92gh	85	29	obtained	obtain	VBN
cord-009376-a35a92gh	85	30	from	from	IN
cord-009376-a35a92gh	85	31	eight	eight	CD
cord-009376-a35a92gh	85	32	negative	negative	JJ
cord-009376-a35a92gh	85	33	controls	control	NNS
cord-009376-a35a92gh	85	34	and	and	CC
cord-009376-a35a92gh	85	35	no	no	DT
cord-009376-a35a92gh	85	36	template	template	NN
cord-009376-a35a92gh	85	37	controls	control	NNS
cord-009376-a35a92gh	85	38	.	.	.
cord-009376-a35a92gh	86	1	Within	within	IN
cord-009376-a35a92gh	86	2	the	the	DT
cord-009376-a35a92gh	86	3	group	group	NN
cord-009376-a35a92gh	86	4	of	of	IN
cord-009376-a35a92gh	86	5	eight	eight	CD
cord-009376-a35a92gh	86	6	replicates	replicate	NNS
cord-009376-a35a92gh	86	7	containing	contain	VBG
cord-009376-a35a92gh	86	8	target	target	NN
cord-009376-a35a92gh	86	9	levels	level	NNS
cord-009376-a35a92gh	86	10	at	at	IN
cord-009376-a35a92gh	86	11	the	the	DT
cord-009376-a35a92gh	86	12	limit	limit	NN
cord-009376-a35a92gh	86	13	of	of	IN
cord-009376-a35a92gh	86	14	detection	detection	NN
cord-009376-a35a92gh	86	15	,	,	,
cord-009376-a35a92gh	86	16	there	there	EX
cord-009376-a35a92gh	86	17	should	should	MD
cord-009376-a35a92gh	86	18	be	be	VB
cord-009376-a35a92gh	86	19	no	no	DT
cord-009376-a35a92gh	86	20	failed	fail	VBN
cord-009376-a35a92gh	86	21	reactions	reaction	NNS
cord-009376-a35a92gh	86	22	,	,	,
cord-009376-a35a92gh	86	23	and	and	CC
cord-009376-a35a92gh	86	24	all	all	DT
cord-009376-a35a92gh	86	25	replicates	replicate	NNS
cord-009376-a35a92gh	86	26	should	should	MD
cord-009376-a35a92gh	86	27	produce	produce	VB
cord-009376-a35a92gh	86	28	amplification	amplification	NN
cord-009376-a35a92gh	86	29	signals	signal	NNS
cord-009376-a35a92gh	86	30	with	with	IN
cord-009376-a35a92gh	86	31	a	a	DT
cord-009376-a35a92gh	86	32	C	c	NN
cord-009376-a35a92gh	86	33	value	value	NN
cord-009376-a35a92gh	86	34	less	less	JJR
cord-009376-a35a92gh	86	35	T	t	NN
cord-009376-a35a92gh	86	36	than	than	IN
cord-009376-a35a92gh	86	37	40	40	CD
cord-009376-a35a92gh	86	38	.	.	.
cord-009376-a35a92gh	87	1	Assay	assay	NN
cord-009376-a35a92gh	87	2	limit	limit	NN
cord-009376-a35a92gh	87	3	of	of	IN
cord-009376-a35a92gh	87	4	detection	detection	NN
cord-009376-a35a92gh	87	5	is	be	VBZ
cord-009376-a35a92gh	87	6	routinely	routinely	RB
cord-009376-a35a92gh	87	7	determined	determine	VBN
cord-009376-a35a92gh	87	8	as	as	IN
cord-009376-a35a92gh	87	9	10	10	CD
cord-009376-a35a92gh	87	10	᎐	᎐	CD
cord-009376-a35a92gh	87	11	100	100	CD
cord-009376-a35a92gh	87	12	nucleic	nucleic	JJ
cord-009376-a35a92gh	87	13	acid	acid	NN
cord-009376-a35a92gh	87	14	copies	copy	NNS
cord-009376-a35a92gh	87	15	in	in	IN
cord-009376-a35a92gh	87	16	a	a	DT
cord-009376-a35a92gh	87	17	Ž	ž	NN
cord-009376-a35a92gh	87	18	background	background	NN
cord-009376-a35a92gh	87	19	of	of	IN
cord-009376-a35a92gh	87	20	200	200	CD
cord-009376-a35a92gh	87	21	000	000	CD
cord-009376-a35a92gh	87	22	cellular	cellular	JJ
cord-009376-a35a92gh	87	23	genomes	genome	NNS
cord-009376-a35a92gh	87	24	for	for	IN
cord-009376-a35a92gh	87	25	DNA	dna	NN
cord-009376-a35a92gh	87	26	.	.	.
cord-009376-a35a92gh	88	1	Ž	ž	NN
cord-009376-a35a92gh	88	2	targets	target	NNS
cord-009376-a35a92gh	88	3	or	or	CC
cord-009376-a35a92gh	88	4	100	100	CD
cord-009376-a35a92gh	88	5	ng	ng	NN
cord-009376-a35a92gh	88	6	of	of	IN
cord-009376-a35a92gh	88	7	cellular	cellular	JJ
cord-009376-a35a92gh	88	8	RNA	RNA	NNP
cord-009376-a35a92gh	88	9	for	for	IN
cord-009376-a35a92gh	88	10	RNA	RNA	NNP
cord-009376-a35a92gh	88	11	.	.	.
cord-009376-a35a92gh	89	1	targets	target	NNS
cord-009376-a35a92gh	89	2	.	.	.
cord-009376-a35a92gh	90	1	Ultimate	ultimate	JJ
cord-009376-a35a92gh	90	2	assessment	assessment	NN
cord-009376-a35a92gh	90	3	of	of	IN
cord-009376-a35a92gh	90	4	assay	assay	NN
cord-009376-a35a92gh	90	5	reproducibility	reproducibility	NN
cord-009376-a35a92gh	90	6	requires	require	VBZ
cord-009376-a35a92gh	90	7	the	the	DT
cord-009376-a35a92gh	90	8	positive	positive	JJ
cord-009376-a35a92gh	90	9	cut	cut	VBN
cord-009376-a35a92gh	90	10	-	-	HYPH
cord-009376-a35a92gh	90	11	off	off	RP
cord-009376-a35a92gh	90	12	point	point	NN
cord-009376-a35a92gh	90	13	to	to	TO
cord-009376-a35a92gh	90	14	be	be	VB
cord-009376-a35a92gh	90	15	determined	determine	VBN
cord-009376-a35a92gh	90	16	.	.	.
cord-009376-a35a92gh	91	1	The	the	DT
cord-009376-a35a92gh	91	2	positive	positive	JJ
cord-009376-a35a92gh	91	3	cut	cut	VBN
cord-009376-a35a92gh	91	4	-	-	HYPH
cord-009376-a35a92gh	91	5	off	off	RP
cord-009376-a35a92gh	91	6	level	level	NN
cord-009376-a35a92gh	91	7	is	be	VBZ
cord-009376-a35a92gh	91	8	the	the	DT
cord-009376-a35a92gh	91	9	minimum	minimum	JJ
cord-009376-a35a92gh	91	10	number	number	NN
cord-009376-a35a92gh	91	11	of	of	IN
cord-009376-a35a92gh	91	12	target	target	NN
cord-009376-a35a92gh	91	13	molecules	molecule	NNS
cord-009376-a35a92gh	91	14	that	that	WDT
cord-009376-a35a92gh	91	15	are	be	VBP
cord-009376-a35a92gh	91	16	detected	detect	VBN
cord-009376-a35a92gh	91	17	in	in	IN
cord-009376-a35a92gh	91	18	95	95	CD
cord-009376-a35a92gh	91	19	%	%	NN
cord-009376-a35a92gh	91	20	of	of	IN
cord-009376-a35a92gh	91	21	test	test	NN
cord-009376-a35a92gh	91	22	runs	run	NNS
cord-009376-a35a92gh	91	23	.	.	.
cord-009376-a35a92gh	92	1	The	the	DT
cord-009376-a35a92gh	92	2	positive	positive	JJ
cord-009376-a35a92gh	92	3	cut	cut	VBN
cord-009376-a35a92gh	92	4	-	-	HYPH
cord-009376-a35a92gh	92	5	off	off	RP
cord-009376-a35a92gh	92	6	point	point	NN
cord-009376-a35a92gh	92	7	of	of	IN
cord-009376-a35a92gh	92	8	an	an	DT
cord-009376-a35a92gh	92	9	assay	assay	NN
cord-009376-a35a92gh	92	10	can	can	MD
cord-009376-a35a92gh	92	11	be	be	VB
cord-009376-a35a92gh	92	12	measured	measure	VBN
cord-009376-a35a92gh	92	13	over	over	IN
cord-009376-a35a92gh	92	14	a	a	DT
cord-009376-a35a92gh	92	15	period	period	NN
cord-009376-a35a92gh	92	16	of	of	IN
cord-009376-a35a92gh	92	17	12	12	CD
cord-009376-a35a92gh	92	18	months	month	NNS
cord-009376-a35a92gh	92	19	using	use	VBG
cord-009376-a35a92gh	92	20	different	different	JJ
cord-009376-a35a92gh	92	21	batches	batch	NNS
cord-009376-a35a92gh	92	22	of	of	IN
cord-009376-a35a92gh	92	23	reagents	reagent	NNS
cord-009376-a35a92gh	92	24	and	and	CC
cord-009376-a35a92gh	92	25	several	several	JJ
cord-009376-a35a92gh	92	26	different	different	JJ
cord-009376-a35a92gh	92	27	operators	operator	NNS
cord-009376-a35a92gh	92	28	.	.	.
cord-009376-a35a92gh	93	1	The	the	DT
cord-009376-a35a92gh	93	2	extraction	extraction	NN
cord-009376-a35a92gh	93	3	of	of	IN
cord-009376-a35a92gh	93	4	viral	viral	JJ
cord-009376-a35a92gh	93	5	nucleic	nucleic	JJ
cord-009376-a35a92gh	93	6	acids	acid	NNS
cord-009376-a35a92gh	93	7	is	be	VBZ
cord-009376-a35a92gh	93	8	widely	widely	RB
cord-009376-a35a92gh	93	9	used	use	VBN
cord-009376-a35a92gh	93	10	in	in	IN
cord-009376-a35a92gh	93	11	diagnostic	diagnostic	JJ
cord-009376-a35a92gh	93	12	and	and	CC
cord-009376-a35a92gh	93	13	research	research	NN
cord-009376-a35a92gh	93	14	laboratories	laboratory	NNS
cord-009376-a35a92gh	93	15	.	.	.
cord-009376-a35a92gh	94	1	However	however	RB
cord-009376-a35a92gh	94	2	,	,	,
cord-009376-a35a92gh	94	3	the	the	DT
cord-009376-a35a92gh	94	4	suitability	suitability	NN
cord-009376-a35a92gh	94	5	or	or	CC
cord-009376-a35a92gh	94	6	yield	yield	NN
cord-009376-a35a92gh	94	7	of	of	IN
cord-009376-a35a92gh	94	8	nucleic	nucleic	JJ
cord-009376-a35a92gh	94	9	acid	acid	NN
cord-009376-a35a92gh	94	10	from	from	IN
cord-009376-a35a92gh	94	11	extraction	extraction	NN
cord-009376-a35a92gh	94	12	procedures	procedure	NNS
cord-009376-a35a92gh	94	13	can	can	MD
cord-009376-a35a92gh	94	14	vary	vary	VB
cord-009376-a35a92gh	94	15	depending	depend	VBG
cord-009376-a35a92gh	94	16	on	on	IN
cord-009376-a35a92gh	94	17	the	the	DT
cord-009376-a35a92gh	94	18	nucleic	nucleic	JJ
cord-009376-a35a92gh	94	19	acid	acid	NN
cord-009376-a35a92gh	94	20	and	and	CC
cord-009376-a35a92gh	94	21	the	the	DT
cord-009376-a35a92gh	94	22	biological	biological	JJ
cord-009376-a35a92gh	94	23	material	material	NN
cord-009376-a35a92gh	94	24	Ž	ž	NN
cord-009376-a35a92gh	94	25	.	.	.
cord-009376-a35a92gh	95	1	Kok	Kok	NNP
cord-009376-a35a92gh	95	2	et	et	NNP
cord-009376-a35a92gh	95	3	al	al	NNP
cord-009376-a35a92gh	95	4	.	.	NNP
cord-009376-a35a92gh	95	5	,	,	,
cord-009376-a35a92gh	95	6	2000	2000	CD
cord-009376-a35a92gh	95	7	.	.	.
cord-009376-a35a92gh	96	1	Therefore	therefore	RB
cord-009376-a35a92gh	96	2	,	,	,
cord-009376-a35a92gh	96	3	to	to	TO
cord-009376-a35a92gh	96	4	ensure	ensure	VB
cord-009376-a35a92gh	96	5	efficient	efficient	JJ
cord-009376-a35a92gh	96	6	extraction	extraction	NN
cord-009376-a35a92gh	96	7	of	of	IN
cord-009376-a35a92gh	96	8	target	target	NN
cord-009376-a35a92gh	96	9	nucleic	nucleic	JJ
cord-009376-a35a92gh	96	10	acid	acid	NN
cord-009376-a35a92gh	96	11	,	,	,
cord-009376-a35a92gh	96	12	the	the	DT
cord-009376-a35a92gh	96	13	procedure	procedure	NN
cord-009376-a35a92gh	96	14	should	should	MD
cord-009376-a35a92gh	96	15	be	be	VB
cord-009376-a35a92gh	96	16	qualified	qualified	JJ
cord-009376-a35a92gh	96	17	for	for	IN
cord-009376-a35a92gh	96	18	the	the	DT
cord-009376-a35a92gh	96	19	particular	particular	JJ
cord-009376-a35a92gh	96	20	target	target	NN
cord-009376-a35a92gh	96	21	and	and	CC
cord-009376-a35a92gh	96	22	biological	biological	JJ
cord-009376-a35a92gh	96	23	material	material	NN
cord-009376-a35a92gh	96	24	to	to	TO
cord-009376-a35a92gh	96	25	be	be	VB
cord-009376-a35a92gh	96	26	used	use	VBN
cord-009376-a35a92gh	96	27	.	.	.
cord-009376-a35a92gh	97	1	Since	since	IN
cord-009376-a35a92gh	97	2	Q	Q	NNP
cord-009376-a35a92gh	97	3	-	-	HYPH
cord-009376-a35a92gh	97	4	PCR	PCR	NNP
cord-009376-a35a92gh	97	5	allows	allow	VBZ
cord-009376-a35a92gh	97	6	the	the	DT
cord-009376-a35a92gh	97	7	quantification	quantification	NN
cord-009376-a35a92gh	97	8	of	of	IN
cord-009376-a35a92gh	97	9	a	a	DT
cord-009376-a35a92gh	97	10	nucleic	nucleic	JJ
cord-009376-a35a92gh	97	11	acid	acid	NN
cord-009376-a35a92gh	97	12	target	target	NN
cord-009376-a35a92gh	97	13	molecule	molecule	NN
cord-009376-a35a92gh	97	14	,	,	,
cord-009376-a35a92gh	97	15	it	-PRON-	PRP
cord-009376-a35a92gh	97	16	can	can	MD
cord-009376-a35a92gh	97	17	be	be	VB
cord-009376-a35a92gh	97	18	used	use	VBN
cord-009376-a35a92gh	97	19	to	to	TO
cord-009376-a35a92gh	97	20	determine	determine	VB
cord-009376-a35a92gh	97	21	the	the	DT
cord-009376-a35a92gh	97	22	percentage	percentage	NN
cord-009376-a35a92gh	97	23	recovery	recovery	NN
cord-009376-a35a92gh	97	24	of	of	IN
cord-009376-a35a92gh	97	25	the	the	DT
cord-009376-a35a92gh	97	26	viral	viral	JJ
cord-009376-a35a92gh	97	27	or	or	CC
cord-009376-a35a92gh	97	28	cellular	cellular	JJ
cord-009376-a35a92gh	97	29	DNA	dna	NN
cord-009376-a35a92gh	97	30	target	target	NN
cord-009376-a35a92gh	97	31	achieved	achieve	VBN
cord-009376-a35a92gh	97	32	during	during	IN
cord-009376-a35a92gh	97	33	the	the	DT
cord-009376-a35a92gh	97	34	extraction	extraction	NN
cord-009376-a35a92gh	97	35	procedure	procedure	NN
cord-009376-a35a92gh	97	36	Ž	ž	NN
cord-009376-a35a92gh	97	37	.	.	.
cord-009376-a35a92gh	98	1	Table	table	NN
cord-009376-a35a92gh	98	2	1	1	CD
cord-009376-a35a92gh	98	3	.	.	.
cord-009376-a35a92gh	99	1	To	to	TO
cord-009376-a35a92gh	99	2	assess	assess	VB
cord-009376-a35a92gh	99	3	further	further	RB
cord-009376-a35a92gh	99	4	the	the	DT
cord-009376-a35a92gh	99	5	performance	performance	NN
cord-009376-a35a92gh	99	6	of	of	IN
cord-009376-a35a92gh	99	7	the	the	DT
cord-009376-a35a92gh	99	8	extraction	extraction	NN
cord-009376-a35a92gh	99	9	procedures	procedure	NNS
cord-009376-a35a92gh	99	10	,	,	,
cord-009376-a35a92gh	99	11	the	the	DT
cord-009376-a35a92gh	99	12	extracted	extract	VBN
cord-009376-a35a92gh	99	13	test	test	NN
cord-009376-a35a92gh	99	14	nucleic	nucleic	JJ
cord-009376-a35a92gh	99	15	acid	acid	NN
cord-009376-a35a92gh	99	16	can	can	MD
cord-009376-a35a92gh	99	17	be	be	VB
cord-009376-a35a92gh	99	18	spiked	spike	VBN
cord-009376-a35a92gh	99	19	with	with	IN
cord-009376-a35a92gh	99	20	target	target	NN
cord-009376-a35a92gh	99	21	molecules	molecule	NNS
cord-009376-a35a92gh	99	22	to	to	TO
cord-009376-a35a92gh	99	23	determine	determine	VB
cord-009376-a35a92gh	99	24	the	the	DT
cord-009376-a35a92gh	99	25	percentage	percentage	NN
cord-009376-a35a92gh	99	26	of	of	IN
cord-009376-a35a92gh	99	27	PCR	PCR	NNP
cord-009376-a35a92gh	99	28	inhibition	inhibition	NN
cord-009376-a35a92gh	99	29	.	.	.
cord-009376-a35a92gh	100	1	The	the	DT
cord-009376-a35a92gh	100	2	calculated	calculate	VBN
cord-009376-a35a92gh	100	3	copy	copy	NN
cord-009376-a35a92gh	100	4	number	number	NN
cord-009376-a35a92gh	100	5	of	of	IN
cord-009376-a35a92gh	100	6	the	the	DT
cord-009376-a35a92gh	100	7	test	test	NN
cord-009376-a35a92gh	100	8	sample	sample	NN
cord-009376-a35a92gh	100	9	is	be	VBZ
cord-009376-a35a92gh	100	10	subsequently	subsequently	RB
cord-009376-a35a92gh	100	11	adjusted	adjust	VBN
cord-009376-a35a92gh	100	12	,	,	,
cord-009376-a35a92gh	100	13	taking	take	VBG
cord-009376-a35a92gh	100	14	into	into	IN
cord-009376-a35a92gh	100	15	account	account	NN
cord-009376-a35a92gh	100	16	the	the	DT
cord-009376-a35a92gh	100	17	amount	amount	NN
cord-009376-a35a92gh	100	18	lost	lose	VBN
cord-009376-a35a92gh	100	19	in	in	IN
cord-009376-a35a92gh	100	20	extraction	extraction	NN
cord-009376-a35a92gh	100	21	and	and	CC
cord-009376-a35a92gh	100	22	the	the	DT
cord-009376-a35a92gh	100	23	estimated	estimate	VBN
cord-009376-a35a92gh	100	24	level	level	NN
cord-009376-a35a92gh	100	25	of	of	IN
cord-009376-a35a92gh	100	26	inhibition	inhibition	NN
cord-009376-a35a92gh	100	27	.	.	.
cord-009376-a35a92gh	101	1	PCR	PCR	NNP
cord-009376-a35a92gh	101	2	inhibition	inhibition	NN
cord-009376-a35a92gh	101	3	is	be	VBZ
cord-009376-a35a92gh	101	4	particularly	particularly	RB
cord-009376-a35a92gh	101	5	important	important	JJ
cord-009376-a35a92gh	101	6	to	to	TO
cord-009376-a35a92gh	101	7	assess	assess	VB
cord-009376-a35a92gh	101	8	when	when	WRB
cord-009376-a35a92gh	101	9	analysing	analyse	VBG
cord-009376-a35a92gh	101	10	many	many	JJ
cord-009376-a35a92gh	101	11	different	different	JJ
cord-009376-a35a92gh	101	12	biological	biological	JJ
cord-009376-a35a92gh	101	13	materials	material	NNS
cord-009376-a35a92gh	101	14	that	that	WDT
cord-009376-a35a92gh	101	15	may	may	MD
cord-009376-a35a92gh	101	16	contain	contain	VB
cord-009376-a35a92gh	101	17	various	various	JJ
cord-009376-a35a92gh	101	18	levels	level	NNS
cord-009376-a35a92gh	101	19	of	of	IN
cord-009376-a35a92gh	101	20	inhibitory	inhibitory	JJ
cord-009376-a35a92gh	101	21	substances	substance	NNS
cord-009376-a35a92gh	101	22	.	.	.
cord-009376-a35a92gh	102	1	When	when	WRB
cord-009376-a35a92gh	102	2	extracting	extract	VBG
cord-009376-a35a92gh	102	3	nucleic	nucleic	JJ
cord-009376-a35a92gh	102	4	acid	acid	NN
cord-009376-a35a92gh	102	5	from	from	IN
cord-009376-a35a92gh	102	6	cell	cell	NN
cord-009376-a35a92gh	102	7	free	free	JJ
cord-009376-a35a92gh	102	8	samples	sample	NNS
cord-009376-a35a92gh	102	9	,	,	,
cord-009376-a35a92gh	102	10	especially	especially	RB
cord-009376-a35a92gh	102	11	final	final	JJ
cord-009376-a35a92gh	102	12	products	product	NNS
cord-009376-a35a92gh	102	13	,	,	,
cord-009376-a35a92gh	102	14	it	-PRON-	PRP
cord-009376-a35a92gh	102	15	is	be	VBZ
cord-009376-a35a92gh	102	16	important	important	JJ
cord-009376-a35a92gh	102	17	to	to	TO
cord-009376-a35a92gh	102	18	include	include	VB
cord-009376-a35a92gh	102	19	a	a	DT
cord-009376-a35a92gh	102	20	carrier	carrier	NN
cord-009376-a35a92gh	102	21	molecule	molecule	NN
cord-009376-a35a92gh	102	22	in	in	IN
cord-009376-a35a92gh	102	23	the	the	DT
cord-009376-a35a92gh	102	24	procedure	procedure	NN
cord-009376-a35a92gh	102	25	.	.	.
cord-009376-a35a92gh	103	1	This	this	DT
cord-009376-a35a92gh	103	2	allows	allow	VBZ
cord-009376-a35a92gh	103	3	maximum	maximum	JJ
cord-009376-a35a92gh	103	4	recovery	recovery	NN
cord-009376-a35a92gh	103	5	of	of	IN
cord-009376-a35a92gh	103	6	low	low	JJ
cord-009376-a35a92gh	103	7	levels	level	NNS
cord-009376-a35a92gh	103	8	of	of	IN
cord-009376-a35a92gh	103	9	nucleic	nucleic	JJ
cord-009376-a35a92gh	103	10	acid	acid	NN
cord-009376-a35a92gh	103	11	and	and	CC
cord-009376-a35a92gh	103	12	is	be	VBZ
cord-009376-a35a92gh	103	13	critical	critical	JJ
cord-009376-a35a92gh	103	14	for	for	IN
cord-009376-a35a92gh	103	15	ensuring	ensure	VBG
cord-009376-a35a92gh	103	16	maximum	maximum	JJ
cord-009376-a35a92gh	103	17	sensitivity	sensitivity	NN
cord-009376-a35a92gh	103	18	of	of	IN
cord-009376-a35a92gh	103	19	the	the	DT
cord-009376-a35a92gh	103	20	PCR	PCR	NNP
cord-009376-a35a92gh	103	21	assay	assay	NN
cord-009376-a35a92gh	103	22	.	.	.
cord-009376-a35a92gh	104	1	This	this	DT
cord-009376-a35a92gh	104	2	is	be	VBZ
cord-009376-a35a92gh	104	3	essential	essential	JJ
cord-009376-a35a92gh	104	4	when	when	WRB
cord-009376-a35a92gh	104	5	screening	screen	VBG
cord-009376-a35a92gh	104	6	vaccines	vaccine	NNS
cord-009376-a35a92gh	104	7	for	for	IN
cord-009376-a35a92gh	104	8	contaminating	contaminate	VBG
cord-009376-a35a92gh	104	9	host	host	NN
cord-009376-a35a92gh	104	10	cell	cell	NN
cord-009376-a35a92gh	104	11	DNA	dna	NN
cord-009376-a35a92gh	104	12	.	.	.
cord-009376-a35a92gh	105	1	As	as	IN
cord-009376-a35a92gh	105	2	determined	determine	VBN
cord-009376-a35a92gh	105	3	by	by	IN
cord-009376-a35a92gh	105	4	Q	Q	NNP
cord-009376-a35a92gh	105	5	-	-	HYPH
cord-009376-a35a92gh	105	6	PCR	PCR	NNP
cord-009376-a35a92gh	105	7	,	,	,
cord-009376-a35a92gh	105	8	typical	typical	JJ
cord-009376-a35a92gh	105	9	extraction	extraction	NN
cord-009376-a35a92gh	105	10	recoveries	recovery	NNS
cord-009376-a35a92gh	105	11	of	of	IN
cord-009376-a35a92gh	105	12	22	22	CD
cord-009376-a35a92gh	105	13	%	%	NN
cord-009376-a35a92gh	105	14	and	and	CC
cord-009376-a35a92gh	105	15	greater	great	JJR
cord-009376-a35a92gh	105	16	can	can	MD
cord-009376-a35a92gh	105	17	be	be	VB
cord-009376-a35a92gh	105	18	achieved	achieve	VBN
cord-009376-a35a92gh	105	19	in	in	IN
cord-009376-a35a92gh	105	20	78	78	CD
cord-009376-a35a92gh	105	21	pg	pg	NN
cord-009376-a35a92gh	105	22	of	of	IN
cord-009376-a35a92gh	105	23	human	human	JJ
cord-009376-a35a92gh	105	24	cell	cell	NN
cord-009376-a35a92gh	105	25	line	line	NN
cord-009376-a35a92gh	105	26	DNA	dna	NN
cord-009376-a35a92gh	105	27	per	per	IN
cord-009376-a35a92gh	105	28	0.5-ml	0.5-ml	CD
cord-009376-a35a92gh	105	29	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	105	30	dose	dose	NN
cord-009376-a35a92gh	105	31	,	,	,
cord-009376-a35a92gh	105	32	using	use	VBG
cord-009376-a35a92gh	105	33	the	the	DT
cord-009376-a35a92gh	105	34	Qiagen	Qiagen	NNP
cord-009376-a35a92gh	105	35	DNA	dna	NN
cord-009376-a35a92gh	105	36	Ž	ž	NN
cord-009376-a35a92gh	105	37	.	.	.
cord-009376-a35a92gh	106	1	mini	mini	NNP
cord-009376-a35a92gh	106	2	kit	kit	NNP
cord-009376-a35a92gh	106	3	Table	Table	NNP
cord-009376-a35a92gh	106	4	1	1	CD
cord-009376-a35a92gh	106	5	.	.	.
cord-009376-a35a92gh	107	1	Similar	similar	JJ
cord-009376-a35a92gh	107	2	results	result	NNS
cord-009376-a35a92gh	107	3	are	be	VBP
cord-009376-a35a92gh	107	4	obtained	obtain	VBN
cord-009376-a35a92gh	107	5	when	when	WRB
cord-009376-a35a92gh	107	6	using	use	VBG
cord-009376-a35a92gh	107	7	Q	Q	NNP
cord-009376-a35a92gh	107	8	-	-	HYPH
cord-009376-a35a92gh	107	9	PCR	PCR	NNP
cord-009376-a35a92gh	107	10	assays	assay	NNS
cord-009376-a35a92gh	107	11	for	for	IN
cord-009376-a35a92gh	107	12	primate	primate	JJ
cord-009376-a35a92gh	107	13	,	,	,
cord-009376-a35a92gh	107	14	rodent	rodent	NN
cord-009376-a35a92gh	107	15	,	,	,
cord-009376-a35a92gh	107	16	chicken	chicken	NN
cord-009376-a35a92gh	107	17	and	and	CC
cord-009376-a35a92gh	107	18	porcine	porcine	JJ
cord-009376-a35a92gh	107	19	cellular	cellular	JJ
cord-009376-a35a92gh	107	20	DNA	dna	NN
cord-009376-a35a92gh	107	21	.	.	.
cord-009376-a35a92gh	108	1	Inhibitory	inhibitory	JJ
cord-009376-a35a92gh	108	2	factors	factor	NNS
cord-009376-a35a92gh	108	3	which	which	WDT
cord-009376-a35a92gh	108	4	interfere	interfere	VBP
cord-009376-a35a92gh	108	5	with	with	IN
cord-009376-a35a92gh	108	6	PCR	PCR	NNP
cord-009376-a35a92gh	108	7	are	be	VBP
cord-009376-a35a92gh	108	8	most	most	RBS
cord-009376-a35a92gh	108	9	apparent	apparent	JJ
cord-009376-a35a92gh	108	10	when	when	WRB
cord-009376-a35a92gh	108	11	performing	perform	VBG
cord-009376-a35a92gh	108	12	extraction	extraction	NN
cord-009376-a35a92gh	108	13	of	of	IN
cord-009376-a35a92gh	108	14	viral	viral	JJ
cord-009376-a35a92gh	108	15	or	or	CC
cord-009376-a35a92gh	108	16	plasmid	plasmid	NN
cord-009376-a35a92gh	108	17	nucleic	nucleic	JJ
cord-009376-a35a92gh	108	18	acid	acid	NN
cord-009376-a35a92gh	108	19	from	from	IN
cord-009376-a35a92gh	108	20	animal	animal	NN
cord-009376-a35a92gh	108	21	tissues	tissue	NNS
cord-009376-a35a92gh	108	22	and	and	CC
cord-009376-a35a92gh	108	23	secretions	secretion	NNS
cord-009376-a35a92gh	108	24	.	.	.
cord-009376-a35a92gh	109	1	Substances	substance	NNS
cord-009376-a35a92gh	109	2	such	such	JJ
cord-009376-a35a92gh	109	3	as	as	IN
cord-009376-a35a92gh	109	4	:	:	:
cord-009376-a35a92gh	109	5	heparin	heparin	NN
cord-009376-a35a92gh	109	6	;	;	:
cord-009376-a35a92gh	109	7	urea	urea	NN
cord-009376-a35a92gh	109	8	and	and	CC
cord-009376-a35a92gh	109	9	haemoglobin	haemoglobin	NN
cord-009376-a35a92gh	109	10	;	;	:
cord-009376-a35a92gh	109	11	ethanol	ethanol	NN
cord-009376-a35a92gh	109	12	;	;	:
cord-009376-a35a92gh	109	13	and	and	CC
cord-009376-a35a92gh	109	14	iso	iso	NN
cord-009376-a35a92gh	109	15	-	-	HYPH
cord-009376-a35a92gh	109	16	propanol	propanol	NN
cord-009376-a35a92gh	109	17	will	will	MD
cord-009376-a35a92gh	109	18	inhibit	inhibit	VB
cord-009376-a35a92gh	109	19	PCR	PCR	NNP
cord-009376-a35a92gh	109	20	and	and	CC
cord-009376-a35a92gh	109	21	affect	affect	VB
cord-009376-a35a92gh	109	22	extraction	extraction	NN
cord-009376-a35a92gh	109	23	efficiency	efficiency	NN
cord-009376-a35a92gh	109	24	Ž	ž	NN
cord-009376-a35a92gh	109	25	.	.	.
cord-009376-a35a92gh	110	1	Qiagen	Qiagen	NNP
cord-009376-a35a92gh	110	2	,	,	,
cord-009376-a35a92gh	110	3	1999	1999	CD
cord-009376-a35a92gh	110	4	.	.	.
cord-009376-a35a92gh	111	1	The	the	DT
cord-009376-a35a92gh	111	2	use	use	NN
cord-009376-a35a92gh	111	3	of	of	IN
cord-009376-a35a92gh	111	4	methods	method	NNS
cord-009376-a35a92gh	111	5	which	which	WDT
cord-009376-a35a92gh	111	6	remove	remove	VBP
cord-009376-a35a92gh	111	7	these	these	DT
cord-009376-a35a92gh	111	8	molecules	molecule	NNS
cord-009376-a35a92gh	111	9	are	be	VBP
cord-009376-a35a92gh	111	10	of	of	IN
cord-009376-a35a92gh	111	11	utmost	utmost	JJ
cord-009376-a35a92gh	111	12	importance	importance	NN
cord-009376-a35a92gh	111	13	when	when	WRB
cord-009376-a35a92gh	111	14	screening	screen	VBG
cord-009376-a35a92gh	111	15	tissue	tissue	NN
cord-009376-a35a92gh	111	16	during	during	IN
cord-009376-a35a92gh	111	17	gene	gene	NN
cord-009376-a35a92gh	111	18	and	and	CC
cord-009376-a35a92gh	111	19	cell	cell	NN
cord-009376-a35a92gh	111	20	therapy	therapy	NN
cord-009376-a35a92gh	111	21	biodistribution	biodistribution	NNP
cord-009376-a35a92gh	111	22	studies	study	NNS
cord-009376-a35a92gh	111	23	.	.	.
cord-009376-a35a92gh	112	1	Therefore	therefore	RB
cord-009376-a35a92gh	112	2	in	in	IN
cord-009376-a35a92gh	112	3	these	these	DT
cord-009376-a35a92gh	112	4	studies	study	NNS
cord-009376-a35a92gh	112	5	,	,	,
cord-009376-a35a92gh	112	6	the	the	DT
cord-009376-a35a92gh	112	7	use	use	NN
cord-009376-a35a92gh	112	8	of	of	IN
cord-009376-a35a92gh	112	9	optimised	optimise	VBN
cord-009376-a35a92gh	112	10	high	high	JJ
cord-009376-a35a92gh	112	11	throughput	throughput	NN
cord-009376-a35a92gh	112	12	nucleic	nucleic	VBZ
cord-009376-a35a92gh	112	13	acid	acid	NN
cord-009376-a35a92gh	112	14	extraction	extraction	NN
cord-009376-a35a92gh	112	15	systems	system	NNS
cord-009376-a35a92gh	112	16	and	and	CC
cord-009376-a35a92gh	112	17	the	the	DT
cord-009376-a35a92gh	112	18	examination	examination	NN
cord-009376-a35a92gh	112	19	of	of	IN
cord-009376-a35a92gh	112	20	the	the	DT
cord-009376-a35a92gh	112	21	recovery	recovery	NN
cord-009376-a35a92gh	112	22	of	of	IN
cord-009376-a35a92gh	112	23	10	10	CD
cord-009376-a35a92gh	112	24	᎐	᎐	CD
cord-009376-a35a92gh	112	25	100	100	CD
cord-009376-a35a92gh	112	26	copies	copy	NNS
cord-009376-a35a92gh	112	27	of	of	IN
cord-009376-a35a92gh	112	28	target	target	NN
cord-009376-a35a92gh	112	29	nucleic	nucleic	JJ
cord-009376-a35a92gh	112	30	acid	acid	NN
cord-009376-a35a92gh	112	31	from	from	IN
cord-009376-a35a92gh	112	32	the	the	DT
cord-009376-a35a92gh	112	33	highest	high	JJS
cord-009376-a35a92gh	112	34	amount	amount	NN
cord-009376-a35a92gh	112	35	of	of	IN
cord-009376-a35a92gh	112	36	tissue	tissue	NN
cord-009376-a35a92gh	112	37	possible	possible	JJ
cord-009376-a35a92gh	112	38	should	should	MD
cord-009376-a35a92gh	112	39	be	be	VB
cord-009376-a35a92gh	112	40	determined	determine	VBN
cord-009376-a35a92gh	112	41	.	.	.
cord-009376-a35a92gh	113	1	Recovery	recovery	NN
cord-009376-a35a92gh	113	2	in	in	IN
cord-009376-a35a92gh	113	3	percentage	percentage	NN
cord-009376-a35a92gh	113	4	values	value	NNS
cord-009376-a35a92gh	113	5	of	of	IN
cord-009376-a35a92gh	113	6	a	a	DT
cord-009376-a35a92gh	113	7	plasmid	plasmid	NN
cord-009376-a35a92gh	113	8	DNA	dna	NN
cord-009376-a35a92gh	113	9	per	per	IN
cord-009376-a35a92gh	113	10	100	100	CD
cord-009376-a35a92gh	113	11	g	g	NN
cord-009376-a35a92gh	113	12	of	of	IN
cord-009376-a35a92gh	113	13	animal	animal	NN
cord-009376-a35a92gh	113	14	Ž	ž	NN
cord-009376-a35a92gh	113	15	.	.	.
cord-009376-a35a92gh	114	1	tissue	tissue	NN
cord-009376-a35a92gh	114	2	are	be	VBP
cord-009376-a35a92gh	114	3	shown	show	VBN
cord-009376-a35a92gh	114	4	Table	Table	NNP
cord-009376-a35a92gh	114	5	2	2	CD
cord-009376-a35a92gh	114	6	.	.	.
cord-009376-a35a92gh	115	1	After	after	IN
cord-009376-a35a92gh	115	2	cycling	cycling	NN
cord-009376-a35a92gh	115	3	is	be	VBZ
cord-009376-a35a92gh	115	4	complete	complete	JJ
cord-009376-a35a92gh	115	5	,	,	,
cord-009376-a35a92gh	115	6	data	datum	NNS
cord-009376-a35a92gh	115	7	are	be	VBP
cord-009376-a35a92gh	115	8	analysed	analyse	VBN
cord-009376-a35a92gh	115	9	in	in	IN
cord-009376-a35a92gh	115	10	real	real	JJ
cord-009376-a35a92gh	115	11	time	time	NN
cord-009376-a35a92gh	115	12	and	and	CC
cord-009376-a35a92gh	115	13	the	the	DT
cord-009376-a35a92gh	115	14	C	C	NNP
cord-009376-a35a92gh	115	15	values	value	NNS
cord-009376-a35a92gh	115	16	of	of	IN
cord-009376-a35a92gh	115	17	each	each	DT
cord-009376-a35a92gh	115	18	replicate	replicate	VBP
cord-009376-a35a92gh	115	19	T	T	NNP
cord-009376-a35a92gh	115	20	observed	observe	VBN
cord-009376-a35a92gh	115	21	and	and	CC
cord-009376-a35a92gh	115	22	recorded	record	VBD
cord-009376-a35a92gh	115	23	.	.	.
cord-009376-a35a92gh	116	1	The	the	DT
cord-009376-a35a92gh	116	2	standard	standard	JJ
cord-009376-a35a92gh	116	3	deviations	deviation	NNS
cord-009376-a35a92gh	116	4	and	and	CC
cord-009376-a35a92gh	116	5	confidence	confidence	NN
cord-009376-a35a92gh	116	6	limits	limit	NNS
cord-009376-a35a92gh	116	7	of	of	IN
cord-009376-a35a92gh	116	8	C	C	NNP
cord-009376-a35a92gh	116	9	values	value	NNS
cord-009376-a35a92gh	116	10	of	of	IN
cord-009376-a35a92gh	116	11	the	the	DT
cord-009376-a35a92gh	116	12	repli	repli	NNP
cord-009376-a35a92gh	116	13	-	-	HYPH
cord-009376-a35a92gh	116	14	T	T	NNP
cord-009376-a35a92gh	116	15	cates	cate	NNS
cord-009376-a35a92gh	116	16	are	be	VBP
cord-009376-a35a92gh	116	17	calculated	calculate	VBN
cord-009376-a35a92gh	116	18	to	to	TO
cord-009376-a35a92gh	116	19	detect	detect	VB
cord-009376-a35a92gh	116	20	similarities	similarity	NNS
cord-009376-a35a92gh	116	21	and	and	CC
cord-009376-a35a92gh	116	22	significant	significant	JJ
cord-009376-a35a92gh	116	23	differences	difference	NNS
cord-009376-a35a92gh	116	24	between	between	IN
cord-009376-a35a92gh	116	25	groups	group	NNS
cord-009376-a35a92gh	116	26	of	of	IN
cord-009376-a35a92gh	116	27	replicates	replicate	NNS
cord-009376-a35a92gh	116	28	.	.	.
cord-009376-a35a92gh	117	1	Firstly	firstly	RB
cord-009376-a35a92gh	117	2	,	,	,
cord-009376-a35a92gh	117	3	it	-PRON-	PRP
cord-009376-a35a92gh	117	4	is	be	VBZ
cord-009376-a35a92gh	117	5	not	not	RB
cord-009376-a35a92gh	117	6	impossible	impossible	JJ
cord-009376-a35a92gh	117	7	that	that	IN
cord-009376-a35a92gh	117	8	a	a	DT
cord-009376-a35a92gh	117	9	positive	positive	JJ
cord-009376-a35a92gh	117	10	result	result	NN
cord-009376-a35a92gh	117	11	may	may	MD
cord-009376-a35a92gh	117	12	derive	derive	VB
cord-009376-a35a92gh	117	13	from	from	IN
cord-009376-a35a92gh	117	14	nucleic	nucleic	JJ
cord-009376-a35a92gh	117	15	acid	acid	NN
cord-009376-a35a92gh	117	16	of	of	IN
cord-009376-a35a92gh	117	17	previously	previously	RB
cord-009376-a35a92gh	117	18	unrecognised	unrecognised	JJ
cord-009376-a35a92gh	117	19	origin	origin	NN
cord-009376-a35a92gh	117	20	that	that	WDT
cord-009376-a35a92gh	117	21	shows	show	VBZ
cord-009376-a35a92gh	117	22	non	non	JJ
cord-009376-a35a92gh	117	23	-	-	JJ
cord-009376-a35a92gh	117	24	specific	specific	JJ
cord-009376-a35a92gh	117	25	interactions	interaction	NNS
cord-009376-a35a92gh	117	26	,	,	,
cord-009376-a35a92gh	117	27	due	due	IN
cord-009376-a35a92gh	117	28	to	to	IN
cord-009376-a35a92gh	117	29	sequence	sequence	NN
cord-009376-a35a92gh	117	30	homology	homology	NN
cord-009376-a35a92gh	117	31	with	with	IN
cord-009376-a35a92gh	117	32	the	the	DT
cord-009376-a35a92gh	117	33	amplification	amplification	NN
cord-009376-a35a92gh	117	34	target	target	NN
cord-009376-a35a92gh	117	35	used	use	VBN
cord-009376-a35a92gh	117	36	in	in	IN
cord-009376-a35a92gh	117	37	detection	detection	NN
cord-009376-a35a92gh	117	38	.	.	.
cord-009376-a35a92gh	118	1	In	in	IN
cord-009376-a35a92gh	118	2	a	a	DT
cord-009376-a35a92gh	118	3	valid	valid	JJ
cord-009376-a35a92gh	118	4	assay	assay	NN
cord-009376-a35a92gh	118	5	,	,	,
cord-009376-a35a92gh	118	6	a	a	DT
cord-009376-a35a92gh	118	7	positive	positive	JJ
cord-009376-a35a92gh	118	8	result	result	NN
cord-009376-a35a92gh	118	9	for	for	IN
cord-009376-a35a92gh	118	10	the	the	DT
cord-009376-a35a92gh	118	11	test	test	NN
cord-009376-a35a92gh	118	12	sample	sample	NN
cord-009376-a35a92gh	118	13	is	be	VBZ
cord-009376-a35a92gh	118	14	considered	consider	VBN
cord-009376-a35a92gh	118	15	to	to	TO
cord-009376-a35a92gh	118	16	have	have	VB
cord-009376-a35a92gh	118	17	occurred	occur	VBN
cord-009376-a35a92gh	118	18	when	when	WRB
cord-009376-a35a92gh	118	19	the	the	DT
cord-009376-a35a92gh	118	20	following	follow	VBG
cord-009376-a35a92gh	118	21	four	four	CD
cord-009376-a35a92gh	118	22	conditions	condition	NNS
cord-009376-a35a92gh	118	23	are	be	VBP
cord-009376-a35a92gh	118	24	met	meet	VBN
cord-009376-a35a92gh	118	25	when	when	WRB
cord-009376-a35a92gh	118	26	testing	test	VBG
cord-009376-a35a92gh	118	27	the	the	DT
cord-009376-a35a92gh	118	28	sample	sample	NN
cord-009376-a35a92gh	118	29	with	with	IN
cord-009376-a35a92gh	118	30	two	two	CD
cord-009376-a35a92gh	118	31	independent	independent	JJ
cord-009376-a35a92gh	118	32	amplimer	amplimer	NN
cord-009376-a35a92gh	118	33	sets	set	NNS
cord-009376-a35a92gh	118	34	to	to	IN
cord-009376-a35a92gh	118	35	the	the	DT
cord-009376-a35a92gh	118	36	specified	specify	VBN
cord-009376-a35a92gh	118	37	target	target	NN
cord-009376-a35a92gh	118	38	.	.	.
cord-009376-a35a92gh	119	1	Ž	Ž	NNP
cord-009376-a35a92gh	119	2	.	.	.
cord-009376-a35a92gh	120	1	1	1	LS
cord-009376-a35a92gh	121	1	There	there	EX
cord-009376-a35a92gh	121	2	are	be	VBP
cord-009376-a35a92gh	121	3	significant	significant	JJ
cord-009376-a35a92gh	121	4	amplification	amplification	NN
cord-009376-a35a92gh	121	5	signals	signal	NNS
cord-009376-a35a92gh	121	6	pre	pre	VBP
cord-009376-a35a92gh	121	7	-	-	NN
cord-009376-a35a92gh	121	8	Ž	ž	NN
cord-009376-a35a92gh	121	9	.	.	.
cord-009376-a35a92gh	122	1	sent	send	VBN
cord-009376-a35a92gh	122	2	in	in	IN
cord-009376-a35a92gh	122	3	the	the	DT
cord-009376-a35a92gh	122	4	test	test	NN
cord-009376-a35a92gh	122	5	sample	sample	NN
cord-009376-a35a92gh	122	6	reactions	reaction	NNS
cord-009376-a35a92gh	122	7	.	.	.
cord-009376-a35a92gh	123	1	2	2	LS
cord-009376-a35a92gh	124	1	There	there	EX
cord-009376-a35a92gh	124	2	are	be	VBP
cord-009376-a35a92gh	124	3	no	no	DT
cord-009376-a35a92gh	124	4	amplification	amplification	NN
cord-009376-a35a92gh	124	5	signals	signal	NNS
cord-009376-a35a92gh	124	6	present	present	JJ
cord-009376-a35a92gh	124	7	in	in	IN
cord-009376-a35a92gh	124	8	the	the	DT
cord-009376-a35a92gh	124	9	sentinel	sentinel	NN
cord-009376-a35a92gh	124	10	con	con	NN
cord-009376-a35a92gh	124	11	-	-	HYPH
cord-009376-a35a92gh	124	12	Ž	ž	NN
cord-009376-a35a92gh	124	13	.	.	.
cord-009376-a35a92gh	124	14	trols	trol	NNS
cord-009376-a35a92gh	124	15	.	.	.
cord-009376-a35a92gh	125	1	3	3	LS
cord-009376-a35a92gh	126	1	There	there	EX
cord-009376-a35a92gh	126	2	are	be	VBP
cord-009376-a35a92gh	126	3	no	no	DT
cord-009376-a35a92gh	126	4	amplification	amplification	NN
cord-009376-a35a92gh	126	5	signals	signal	NNS
cord-009376-a35a92gh	126	6	pre	pre	VBP
cord-009376-a35a92gh	126	7	-	-	NN
cord-009376-a35a92gh	126	8	Ž	ž	NN
cord-009376-a35a92gh	126	9	.	.	.
cord-009376-a35a92gh	127	1	sent	send	VBN
cord-009376-a35a92gh	127	2	in	in	IN
cord-009376-a35a92gh	127	3	the	the	DT
cord-009376-a35a92gh	127	4	negative	negative	JJ
cord-009376-a35a92gh	127	5	controls	control	NNS
cord-009376-a35a92gh	127	6	.	.	.
cord-009376-a35a92gh	128	1	4	4	LS
cord-009376-a35a92gh	129	1	There	there	EX
cord-009376-a35a92gh	129	2	are	be	VBP
cord-009376-a35a92gh	129	3	significant	significant	JJ
cord-009376-a35a92gh	129	4	amplification	amplification	NN
cord-009376-a35a92gh	129	5	signals	signal	NNS
cord-009376-a35a92gh	129	6	present	present	JJ
cord-009376-a35a92gh	129	7	in	in	IN
cord-009376-a35a92gh	129	8	the	the	DT
cord-009376-a35a92gh	129	9	test	test	NN
cord-009376-a35a92gh	129	10	article	article	NN
cord-009376-a35a92gh	129	11	spiked	spike	VBD
cord-009376-a35a92gh	129	12	at	at	IN
cord-009376-a35a92gh	129	13	the	the	DT
cord-009376-a35a92gh	129	14	limit	limit	NN
cord-009376-a35a92gh	129	15	of	of	IN
cord-009376-a35a92gh	129	16	detection	detection	NN
cord-009376-a35a92gh	129	17	.	.	.
cord-009376-a35a92gh	130	1	Detection	detection	NN
cord-009376-a35a92gh	130	2	of	of	IN
cord-009376-a35a92gh	130	3	the	the	DT
cord-009376-a35a92gh	130	4	target	target	NN
cord-009376-a35a92gh	130	5	sequence	sequence	NN
cord-009376-a35a92gh	130	6	by	by	IN
cord-009376-a35a92gh	130	7	PCR	PCR	NNP
cord-009376-a35a92gh	130	8	in	in	IN
cord-009376-a35a92gh	130	9	any	any	DT
cord-009376-a35a92gh	130	10	of	of	IN
cord-009376-a35a92gh	130	11	the	the	DT
cord-009376-a35a92gh	130	12	sentinel	sentinel	JJ
cord-009376-a35a92gh	130	13	or	or	CC
cord-009376-a35a92gh	130	14	negative	negative	JJ
cord-009376-a35a92gh	130	15	controls	control	NNS
cord-009376-a35a92gh	130	16	invalidates	invalidate	VBZ
cord-009376-a35a92gh	130	17	the	the	DT
cord-009376-a35a92gh	130	18	assay	assay	NN
cord-009376-a35a92gh	130	19	and	and	CC
cord-009376-a35a92gh	130	20	is	be	VBZ
cord-009376-a35a92gh	130	21	indicative	indicative	JJ
cord-009376-a35a92gh	130	22	of	of	IN
cord-009376-a35a92gh	130	23	contamination	contamination	NN
cord-009376-a35a92gh	130	24	.	.	.
cord-009376-a35a92gh	131	1	In	in	IN
cord-009376-a35a92gh	131	2	a	a	DT
cord-009376-a35a92gh	131	3	positive	positive	JJ
cord-009376-a35a92gh	131	4	test	test	NN
cord-009376-a35a92gh	131	5	with	with	IN
cord-009376-a35a92gh	131	6	confidence	confidence	NN
cord-009376-a35a92gh	131	7	,	,	,
cord-009376-a35a92gh	131	8	analysis	analysis	NN
cord-009376-a35a92gh	131	9	of	of	IN
cord-009376-a35a92gh	131	10	data	data	NN
cord-009376-a35a92gh	131	11	results	result	NNS
cord-009376-a35a92gh	131	12	in	in	IN
cord-009376-a35a92gh	131	13	the	the	DT
cord-009376-a35a92gh	131	14	C	C	NNP
cord-009376-a35a92gh	131	15	values	value	NNS
cord-009376-a35a92gh	131	16	of	of	IN
cord-009376-a35a92gh	131	17	the	the	DT
cord-009376-a35a92gh	131	18	test	test	NN
cord-009376-a35a92gh	131	19	sample	sample	NN
cord-009376-a35a92gh	131	20	,	,	,
cord-009376-a35a92gh	131	21	spiked	spike	VBD
cord-009376-a35a92gh	131	22	T	t	NN
cord-009376-a35a92gh	131	23	test	test	NN
cord-009376-a35a92gh	131	24	sample	sample	NN
cord-009376-a35a92gh	131	25	and	and	CC
cord-009376-a35a92gh	131	26	positive	positive	JJ
cord-009376-a35a92gh	131	27	control	control	NN
cord-009376-a35a92gh	131	28	samples	sample	NNS
cord-009376-a35a92gh	131	29	being	be	VBG
cord-009376-a35a92gh	131	30	significantly	significantly	RB
cord-009376-a35a92gh	131	31	less	less	JJR
cord-009376-a35a92gh	131	32	than	than	IN
cord-009376-a35a92gh	131	33	the	the	DT
cord-009376-a35a92gh	131	34	negativersentinel	negativersentinel	NNP
cord-009376-a35a92gh	131	35	reactions	reaction	NNS
cord-009376-a35a92gh	131	36	.	.	.
cord-009376-a35a92gh	132	1	When	when	WRB
cord-009376-a35a92gh	132	2	the	the	DT
cord-009376-a35a92gh	132	3	level	level	NN
cord-009376-a35a92gh	132	4	of	of	IN
cord-009376-a35a92gh	132	5	target	target	NN
cord-009376-a35a92gh	132	6	molecules	molecule	NNS
cord-009376-a35a92gh	132	7	is	be	VBZ
cord-009376-a35a92gh	132	8	below	below	IN
cord-009376-a35a92gh	132	9	the	the	DT
cord-009376-a35a92gh	132	10	limit	limit	NN
cord-009376-a35a92gh	132	11	of	of	IN
cord-009376-a35a92gh	132	12	detection	detection	NN
cord-009376-a35a92gh	132	13	,	,	,
cord-009376-a35a92gh	132	14	positive	positive	JJ
cord-009376-a35a92gh	132	15	reactions	reaction	NNS
cord-009376-a35a92gh	132	16	can	can	MD
cord-009376-a35a92gh	132	17	occur	occur	VB
cord-009376-a35a92gh	132	18	in	in	IN
cord-009376-a35a92gh	132	19	one	one	CD
cord-009376-a35a92gh	132	20	of	of	IN
cord-009376-a35a92gh	132	21	three	three	CD
cord-009376-a35a92gh	132	22	or	or	CC
cord-009376-a35a92gh	132	23	two	two	CD
cord-009376-a35a92gh	132	24	of	of	IN
cord-009376-a35a92gh	132	25	three	three	CD
cord-009376-a35a92gh	132	26	replicates	replicate	NNS
cord-009376-a35a92gh	132	27	.	.	.
cord-009376-a35a92gh	133	1	Because	because	IN
cord-009376-a35a92gh	133	2	negative	negative	JJ
cord-009376-a35a92gh	133	3	replicates	replicate	NNS
cord-009376-a35a92gh	133	4	produce	produce	VBP
cord-009376-a35a92gh	133	5	a	a	DT
cord-009376-a35a92gh	133	6	C	c	NN
cord-009376-a35a92gh	133	7	s	s	NN
cord-009376-a35a92gh	133	8	40	40	CD
cord-009376-a35a92gh	133	9	,	,	,
cord-009376-a35a92gh	133	10	the	the	DT
cord-009376-a35a92gh	133	11	T	T	NNP
cord-009376-a35a92gh	133	12	confidence	confidence	NN
cord-009376-a35a92gh	133	13	interval	interval	NN
cord-009376-a35a92gh	133	14	of	of	IN
cord-009376-a35a92gh	133	15	the	the	DT
cord-009376-a35a92gh	133	16	test	test	NN
cord-009376-a35a92gh	133	17	sample	sample	NN
cord-009376-a35a92gh	133	18	is	be	VBZ
cord-009376-a35a92gh	133	19	not	not	RB
cord-009376-a35a92gh	133	20	significantly	significantly	RB
cord-009376-a35a92gh	133	21	different	different	JJ
cord-009376-a35a92gh	133	22	from	from	IN
cord-009376-a35a92gh	133	23	the	the	DT
cord-009376-a35a92gh	133	24	negative	negative	JJ
cord-009376-a35a92gh	133	25	control	control	NN
cord-009376-a35a92gh	133	26	reactions	reaction	NNS
cord-009376-a35a92gh	133	27	.	.	.
cord-009376-a35a92gh	134	1	In	in	IN
cord-009376-a35a92gh	134	2	this	this	DT
cord-009376-a35a92gh	134	3	case	case	NN
cord-009376-a35a92gh	134	4	,	,	,
cord-009376-a35a92gh	134	5	a	a	DT
cord-009376-a35a92gh	134	6	confidence	confidence	NN
cord-009376-a35a92gh	134	7	interval	interval	NN
cord-009376-a35a92gh	134	8	that	that	WDT
cord-009376-a35a92gh	134	9	contains	contain	VBZ
cord-009376-a35a92gh	134	10	positive	positive	JJ
cord-009376-a35a92gh	134	11	amplification	amplification	NN
cord-009376-a35a92gh	134	12	signals	signal	NNS
cord-009376-a35a92gh	134	13	in	in	IN
cord-009376-a35a92gh	134	14	test	test	NN
cord-009376-a35a92gh	134	15	sample	sample	NN
cord-009376-a35a92gh	134	16	reactions	reaction	NNS
cord-009376-a35a92gh	134	17	is	be	VBZ
cord-009376-a35a92gh	134	18	produced	produce	VBN
cord-009376-a35a92gh	134	19	,	,	,
cord-009376-a35a92gh	134	20	but	but	CC
cord-009376-a35a92gh	134	21	overlaps	overlap	VBZ
cord-009376-a35a92gh	134	22	with	with	IN
cord-009376-a35a92gh	134	23	the	the	DT
cord-009376-a35a92gh	134	24	negative	negative	JJ
cord-009376-a35a92gh	134	25	controls	control	NNS
cord-009376-a35a92gh	134	26	.	.	.
cord-009376-a35a92gh	135	1	The	the	DT
cord-009376-a35a92gh	135	2	result	result	NN
cord-009376-a35a92gh	135	3	could	could	MD
cord-009376-a35a92gh	135	4	therefore	therefore	RB
cord-009376-a35a92gh	135	5	be	be	VB
cord-009376-a35a92gh	135	6	considered	consider	VBN
cord-009376-a35a92gh	135	7	'	'	``
cord-009376-a35a92gh	135	8	equivocal	equivocal	JJ
cord-009376-a35a92gh	135	9	with	with	IN
cord-009376-a35a92gh	135	10	signals	signal	NNS
cord-009376-a35a92gh	135	11	detected	detect	VBN
cord-009376-a35a92gh	135	12	below	below	IN
cord-009376-a35a92gh	135	13	the	the	DT
cord-009376-a35a92gh	135	14	validated	validated	JJ
cord-009376-a35a92gh	135	15	assay	assay	NN
cord-009376-a35a92gh	135	16	sensitivity	sensitivity	NN
cord-009376-a35a92gh	135	17	'	'	''
cord-009376-a35a92gh	135	18	.	.	.
cord-009376-a35a92gh	136	1	Increases	increase	NNS
cord-009376-a35a92gh	136	2	in	in	IN
cord-009376-a35a92gh	136	3	sample	sample	NN
cord-009376-a35a92gh	136	4	volume	volume	NN
cord-009376-a35a92gh	136	5	can	can	MD
cord-009376-a35a92gh	136	6	be	be	VB
cord-009376-a35a92gh	136	7	analysed	analyse	VBN
cord-009376-a35a92gh	136	8	,	,	,
cord-009376-a35a92gh	136	9	or	or	CC
cord-009376-a35a92gh	136	10	if	if	IN
cord-009376-a35a92gh	136	11	appropriate	appropriate	JJ
cord-009376-a35a92gh	136	12	,	,	,
cord-009376-a35a92gh	136	13	testing	testing	NN
cord-009376-a35a92gh	136	14	carried	carry	VBN
cord-009376-a35a92gh	136	15	out	out	RP
cord-009376-a35a92gh	136	16	by	by	IN
cord-009376-a35a92gh	136	17	an	an	DT
cord-009376-a35a92gh	136	18	independent	independent	JJ
cord-009376-a35a92gh	136	19	procedure	procedure	NN
cord-009376-a35a92gh	136	20	,	,	,
cord-009376-a35a92gh	136	21	such	such	JJ
cord-009376-a35a92gh	136	22	as	as	IN
cord-009376-a35a92gh	136	23	cell	cell	NN
cord-009376-a35a92gh	136	24	infectivity	infectivity	NN
cord-009376-a35a92gh	136	25	with	with	IN
cord-009376-a35a92gh	136	26	Q	Q	NNP
cord-009376-a35a92gh	136	27	-	-	HYPH
cord-009376-a35a92gh	136	28	PCR	PCR	NNP
cord-009376-a35a92gh	136	29	end	end	NN
cord-009376-a35a92gh	136	30	point	point	NN
cord-009376-a35a92gh	136	31	analysis	analysis	NN
cord-009376-a35a92gh	137	1	In	in	IN
cord-009376-a35a92gh	137	2	a	a	DT
cord-009376-a35a92gh	137	3	valid	valid	JJ
cord-009376-a35a92gh	137	4	assay	assay	NN
cord-009376-a35a92gh	137	5	,	,	,
cord-009376-a35a92gh	137	6	a	a	DT
cord-009376-a35a92gh	137	7	negative	negative	JJ
cord-009376-a35a92gh	137	8	result	result	NN
cord-009376-a35a92gh	137	9	for	for	IN
cord-009376-a35a92gh	137	10	the	the	DT
cord-009376-a35a92gh	137	11	test	test	NN
cord-009376-a35a92gh	137	12	sample	sample	NN
cord-009376-a35a92gh	137	13	is	be	VBZ
cord-009376-a35a92gh	137	14	considered	consider	VBN
cord-009376-a35a92gh	137	15	to	to	TO
cord-009376-a35a92gh	137	16	have	have	VB
cord-009376-a35a92gh	137	17	occurred	occur	VBN
cord-009376-a35a92gh	137	18	when	when	WRB
cord-009376-a35a92gh	137	19	the	the	DT
cord-009376-a35a92gh	137	20	following	follow	VBG
cord-009376-a35a92gh	137	21	four	four	CD
cord-009376-a35a92gh	137	22	conditions	condition	NNS
cord-009376-a35a92gh	137	23	are	be	VBP
cord-009376-a35a92gh	137	24	all	all	DT
cord-009376-a35a92gh	137	25	met	meet	VBN
cord-009376-a35a92gh	137	26	when	when	WRB
cord-009376-a35a92gh	137	27	testing	test	VBG
cord-009376-a35a92gh	137	28	Ž	ž	NN
cord-009376-a35a92gh	137	29	.	.	.
cord-009376-a35a92gh	138	1	the	the	DT
cord-009376-a35a92gh	138	2	sample	sample	NN
cord-009376-a35a92gh	138	3	.	.	.
cord-009376-a35a92gh	139	1	1	1	LS
cord-009376-a35a92gh	140	1	There	there	EX
cord-009376-a35a92gh	140	2	are	be	VBP
cord-009376-a35a92gh	140	3	no	no	DT
cord-009376-a35a92gh	140	4	amplification	amplification	NN
cord-009376-a35a92gh	140	5	signals	signal	NNS
cord-009376-a35a92gh	140	6	Ž	Ž	NNP
cord-009376-a35a92gh	140	7	.	.	.
cord-009376-a35a92gh	141	1	present	present	JJ
cord-009376-a35a92gh	141	2	in	in	IN
cord-009376-a35a92gh	141	3	the	the	DT
cord-009376-a35a92gh	141	4	test	test	NN
cord-009376-a35a92gh	141	5	sample	sample	NN
cord-009376-a35a92gh	141	6	reactions	reaction	NNS
cord-009376-a35a92gh	141	7	.	.	.
cord-009376-a35a92gh	142	1	2	2	LS
cord-009376-a35a92gh	143	1	There	there	EX
cord-009376-a35a92gh	143	2	are	be	VBP
cord-009376-a35a92gh	143	3	no	no	DT
cord-009376-a35a92gh	143	4	amplification	amplification	NN
cord-009376-a35a92gh	143	5	signals	signal	NNS
cord-009376-a35a92gh	143	6	present	present	JJ
cord-009376-a35a92gh	143	7	in	in	IN
cord-009376-a35a92gh	143	8	the	the	DT
cord-009376-a35a92gh	143	9	sentinel	sentinel	JJ
cord-009376-a35a92gh	143	10	Ž	ž	NN
cord-009376-a35a92gh	143	11	.	.	.
cord-009376-a35a92gh	144	1	controls	control	NNS
cord-009376-a35a92gh	144	2	.	.	.
cord-009376-a35a92gh	145	1	3	3	LS
cord-009376-a35a92gh	146	1	There	there	EX
cord-009376-a35a92gh	146	2	are	be	VBP
cord-009376-a35a92gh	146	3	no	no	DT
cord-009376-a35a92gh	146	4	amplification	amplification	NN
cord-009376-a35a92gh	146	5	signals	signal	NNS
cord-009376-a35a92gh	146	6	Ž	Ž	NNP
cord-009376-a35a92gh	146	7	.	.	.
cord-009376-a35a92gh	147	1	present	present	JJ
cord-009376-a35a92gh	147	2	in	in	IN
cord-009376-a35a92gh	147	3	the	the	DT
cord-009376-a35a92gh	147	4	negative	negative	JJ
cord-009376-a35a92gh	147	5	controls	control	NNS
cord-009376-a35a92gh	147	6	.	.	.
cord-009376-a35a92gh	148	1	4	4	LS
cord-009376-a35a92gh	149	1	There	there	EX
cord-009376-a35a92gh	149	2	are	be	VBP
cord-009376-a35a92gh	149	3	amplification	amplification	NN
cord-009376-a35a92gh	149	4	signals	signal	NNS
cord-009376-a35a92gh	149	5	detected	detect	VBN
cord-009376-a35a92gh	149	6	in	in	IN
cord-009376-a35a92gh	149	7	the	the	DT
cord-009376-a35a92gh	149	8	all	all	DT
cord-009376-a35a92gh	149	9	spiked	spiked	JJ
cord-009376-a35a92gh	149	10	positive	positive	JJ
cord-009376-a35a92gh	149	11	control	control	NN
cord-009376-a35a92gh	149	12	reactions	reaction	NNS
cord-009376-a35a92gh	149	13	.	.	.
cord-009376-a35a92gh	150	1	In	in	IN
cord-009376-a35a92gh	150	2	a	a	DT
cord-009376-a35a92gh	150	3	negative	negative	JJ
cord-009376-a35a92gh	150	4	test	test	NN
cord-009376-a35a92gh	150	5	with	with	IN
cord-009376-a35a92gh	150	6	confidence	confidence	NN
cord-009376-a35a92gh	150	7	,	,	,
cord-009376-a35a92gh	150	8	analysis	analysis	NN
cord-009376-a35a92gh	150	9	of	of	IN
cord-009376-a35a92gh	150	10	the	the	DT
cord-009376-a35a92gh	150	11	data	datum	NNS
cord-009376-a35a92gh	150	12	should	should	MD
cord-009376-a35a92gh	150	13	result	result	VB
cord-009376-a35a92gh	150	14	in	in	IN
cord-009376-a35a92gh	150	15	the	the	DT
cord-009376-a35a92gh	150	16	C	C	NNP
cord-009376-a35a92gh	150	17	value	value	NN
cord-009376-a35a92gh	150	18	of	of	IN
cord-009376-a35a92gh	150	19	the	the	DT
cord-009376-a35a92gh	150	20	T	T	NNP
cord-009376-a35a92gh	150	21	sentinel	sentinel	NNP
cord-009376-a35a92gh	150	22	controls	control	NNS
cord-009376-a35a92gh	150	23	,	,	,
cord-009376-a35a92gh	150	24	negative	negative	JJ
cord-009376-a35a92gh	150	25	controls	control	NNS
cord-009376-a35a92gh	150	26	and	and	CC
cord-009376-a35a92gh	150	27	the	the	DT
cord-009376-a35a92gh	150	28	test	test	NN
cord-009376-a35a92gh	150	29	article	article	NN
cord-009376-a35a92gh	150	30	being	be	VBG
cord-009376-a35a92gh	150	31	significantly	significantly	RB
cord-009376-a35a92gh	150	32	greater	great	JJR
cord-009376-a35a92gh	150	33	than	than	IN
cord-009376-a35a92gh	150	34	those	those	DT
cord-009376-a35a92gh	150	35	from	from	IN
cord-009376-a35a92gh	150	36	the	the	DT
cord-009376-a35a92gh	150	37	test	test	NN
cord-009376-a35a92gh	150	38	article	article	NN
cord-009376-a35a92gh	150	39	spiked	spike	VBD
cord-009376-a35a92gh	150	40	at	at	IN
cord-009376-a35a92gh	150	41	the	the	DT
cord-009376-a35a92gh	150	42	limit	limit	NN
cord-009376-a35a92gh	150	43	of	of	IN
cord-009376-a35a92gh	150	44	detection	detection	NN
cord-009376-a35a92gh	150	45	.	.	.
cord-009376-a35a92gh	151	1	Occasionally	occasionally	RB
cord-009376-a35a92gh	151	2	,	,	,
cord-009376-a35a92gh	151	3	one	one	CD
cord-009376-a35a92gh	151	4	in	in	IN
cord-009376-a35a92gh	151	5	three	three	CD
cord-009376-a35a92gh	151	6	,	,	,
cord-009376-a35a92gh	151	7	or	or	CC
cord-009376-a35a92gh	151	8	two	two	CD
cord-009376-a35a92gh	151	9	in	in	IN
cord-009376-a35a92gh	151	10	three	three	CD
cord-009376-a35a92gh	151	11	replicates	replicate	NNS
cord-009376-a35a92gh	151	12	of	of	IN
cord-009376-a35a92gh	151	13	the	the	DT
cord-009376-a35a92gh	151	14	test	test	NN
cord-009376-a35a92gh	151	15	sample	sample	NN
cord-009376-a35a92gh	151	16	spiked	spike	VBN
cord-009376-a35a92gh	151	17	at	at	IN
cord-009376-a35a92gh	151	18	the	the	DT
cord-009376-a35a92gh	151	19	limit	limit	NN
cord-009376-a35a92gh	151	20	of	of	IN
cord-009376-a35a92gh	151	21	(	(	-LRB-
cord-009376-a35a92gh	151	22	)	)	-RRB-
cord-009376-a35a92gh	151	23	detection	detection	NN
cord-009376-a35a92gh	151	24	may	may	MD
cord-009376-a35a92gh	151	25	contain	contain	VB
cord-009376-a35a92gh	151	26	C	C	NNP
cord-009376-a35a92gh	151	27	s	s	NNP
cord-009376-a35a92gh	151	28	40	40	CD
cord-009376-a35a92gh	151	29	.	.	.
cord-009376-a35a92gh	152	1	If	if	IN
cord-009376-a35a92gh	152	2	no	no	DT
cord-009376-a35a92gh	152	3	amplifica	amplifica	NNP
cord-009376-a35a92gh	152	4	-	-	HYPH
cord-009376-a35a92gh	152	5	T	T	NNP
cord-009376-a35a92gh	152	6	tions	tion	NNS
cord-009376-a35a92gh	152	7	are	be	VBP
cord-009376-a35a92gh	152	8	detected	detect	VBN
cord-009376-a35a92gh	152	9	in	in	IN
cord-009376-a35a92gh	152	10	the	the	DT
cord-009376-a35a92gh	152	11	test	test	NN
cord-009376-a35a92gh	152	12	article	article	NN
cord-009376-a35a92gh	152	13	,	,	,
cord-009376-a35a92gh	152	14	the	the	DT
cord-009376-a35a92gh	152	15	confidence	confidence	NN
cord-009376-a35a92gh	152	16	intervals	interval	NNS
cord-009376-a35a92gh	152	17	show	show	VBP
cord-009376-a35a92gh	152	18	that	that	IN
cord-009376-a35a92gh	152	19	the	the	DT
cord-009376-a35a92gh	152	20	spiked	spiked	JJ
cord-009376-a35a92gh	152	21	data	datum	NNS
cord-009376-a35a92gh	152	22	is	be	VBZ
cord-009376-a35a92gh	152	23	not	not	RB
cord-009376-a35a92gh	152	24	significantly	significantly	RB
cord-009376-a35a92gh	152	25	different	different	JJ
cord-009376-a35a92gh	152	26	from	from	IN
cord-009376-a35a92gh	152	27	the	the	DT
cord-009376-a35a92gh	152	28	test	test	NN
cord-009376-a35a92gh	152	29	article	article	NN
cord-009376-a35a92gh	152	30	and	and	CC
cord-009376-a35a92gh	152	31	negative	negative	JJ
cord-009376-a35a92gh	152	32	control	control	NN
cord-009376-a35a92gh	152	33	data	datum	NNS
cord-009376-a35a92gh	152	34	.	.	.
cord-009376-a35a92gh	153	1	In	in	IN
cord-009376-a35a92gh	153	2	this	this	DT
cord-009376-a35a92gh	153	3	case	case	NN
cord-009376-a35a92gh	153	4	,	,	,
cord-009376-a35a92gh	153	5	the	the	DT
cord-009376-a35a92gh	153	6	result	result	NN
cord-009376-a35a92gh	153	7	would	would	MD
cord-009376-a35a92gh	153	8	be	be	VB
cord-009376-a35a92gh	153	9	considered	consider	VBN
cord-009376-a35a92gh	153	10	negative	negative	JJ
cord-009376-a35a92gh	153	11	below	below	IN
cord-009376-a35a92gh	153	12	confidence	confidence	NN
cord-009376-a35a92gh	153	13	,	,	,
cord-009376-a35a92gh	153	14	since	since	IN
cord-009376-a35a92gh	153	15	the	the	DT
cord-009376-a35a92gh	153	16	test	test	NN
cord-009376-a35a92gh	153	17	was	be	VBD
cord-009376-a35a92gh	153	18	not	not	RB
cord-009376-a35a92gh	153	19	able	able	JJ
cord-009376-a35a92gh	153	20	to	to	TO
cord-009376-a35a92gh	153	21	detect	detect	VB
cord-009376-a35a92gh	153	22	amplification	amplification	NN
cord-009376-a35a92gh	153	23	signals	signal	NNS
cord-009376-a35a92gh	153	24	in	in	IN
cord-009376-a35a92gh	153	25	all	all	DT
cord-009376-a35a92gh	153	26	of	of	IN
cord-009376-a35a92gh	153	27	the	the	DT
cord-009376-a35a92gh	153	28	spiked	spiked	JJ
cord-009376-a35a92gh	153	29	replicates	replicate	NNS
cord-009376-a35a92gh	153	30	.	.	.
cord-009376-a35a92gh	154	1	The	the	DT
cord-009376-a35a92gh	154	2	accuracy	accuracy	NN
cord-009376-a35a92gh	154	3	of	of	IN
cord-009376-a35a92gh	154	4	the	the	DT
cord-009376-a35a92gh	154	5	quantitative	quantitative	JJ
cord-009376-a35a92gh	154	6	data	datum	NNS
cord-009376-a35a92gh	154	7	for	for	IN
cord-009376-a35a92gh	154	8	a	a	DT
cord-009376-a35a92gh	154	9	number	number	NN
cord-009376-a35a92gh	154	10	of	of	IN
cord-009376-a35a92gh	154	11	Q	Q	NNP
cord-009376-a35a92gh	154	12	-	-	HYPH
cord-009376-a35a92gh	154	13	PCR	PCR	NNP
cord-009376-a35a92gh	154	14	assays	assay	NNS
cord-009376-a35a92gh	154	15	can	can	MD
cord-009376-a35a92gh	154	16	be	be	VB
cord-009376-a35a92gh	154	17	determined	determine	VBN
cord-009376-a35a92gh	154	18	using	use	VBG
cord-009376-a35a92gh	154	19	a	a	DT
cord-009376-a35a92gh	154	20	minimum	minimum	NN
cord-009376-a35a92gh	154	21	of	of	IN
cord-009376-a35a92gh	154	22	eight	eight	CD
cord-009376-a35a92gh	154	23	replicates	replicate	NNS
cord-009376-a35a92gh	154	24	containing	contain	VBG
cord-009376-a35a92gh	154	25	identical	identical	JJ
cord-009376-a35a92gh	154	26	amounts	amount	NNS
cord-009376-a35a92gh	154	27	of	of	IN
cord-009376-a35a92gh	154	28	DNA	dna	NN
cord-009376-a35a92gh	154	29	or	or	CC
cord-009376-a35a92gh	154	30	viral	viral	JJ
cord-009376-a35a92gh	154	31	RNA	RNA	NNP
cord-009376-a35a92gh	154	32	spread	spread	VBD
cord-009376-a35a92gh	154	33	across	across	IN
cord-009376-a35a92gh	154	34	a	a	DT
cord-009376-a35a92gh	154	35	range	range	NN
cord-009376-a35a92gh	154	36	of	of	IN
cord-009376-a35a92gh	154	37	concentrations	concentration	NNS
cord-009376-a35a92gh	154	38	.	.	.
cord-009376-a35a92gh	155	1	During	during	IN
cord-009376-a35a92gh	155	2	the	the	DT
cord-009376-a35a92gh	155	3	analysis	analysis	NN
cord-009376-a35a92gh	155	4	,	,	,
cord-009376-a35a92gh	155	5	four	four	CD
cord-009376-a35a92gh	155	6	of	of	IN
cord-009376-a35a92gh	155	7	the	the	DT
cord-009376-a35a92gh	155	8	replicates	replicate	NNS
cord-009376-a35a92gh	155	9	are	be	VBP
cord-009376-a35a92gh	155	10	termed	term	VBN
cord-009376-a35a92gh	155	11	standards	standard	NNS
cord-009376-a35a92gh	155	12	and	and	CC
cord-009376-a35a92gh	155	13	the	the	DT
cord-009376-a35a92gh	155	14	other	other	JJ
cord-009376-a35a92gh	155	15	four	four	CD
cord-009376-a35a92gh	155	16	replicates	replicate	NNS
cord-009376-a35a92gh	155	17	designated	designate	VBN
cord-009376-a35a92gh	155	18	unknowns	unknown	NNS
cord-009376-a35a92gh	155	19	.	.	.
cord-009376-a35a92gh	156	1	After	after	IN
cord-009376-a35a92gh	156	2	generation	generation	NN
cord-009376-a35a92gh	156	3	of	of	IN
cord-009376-a35a92gh	156	4	the	the	DT
cord-009376-a35a92gh	156	5	standard	standard	JJ
cord-009376-a35a92gh	156	6	curve	curve	NN
cord-009376-a35a92gh	156	7	and	and	CC
cord-009376-a35a92gh	156	8	quantification	quantification	NN
cord-009376-a35a92gh	156	9	of	of	IN
cord-009376-a35a92gh	156	10	the	the	DT
cord-009376-a35a92gh	156	11	unknowns	unknown	NNS
cord-009376-a35a92gh	156	12	,	,	,
cord-009376-a35a92gh	156	13	the	the	DT
cord-009376-a35a92gh	156	14	accuracy	accuracy	NN
cord-009376-a35a92gh	156	15	of	of	IN
cord-009376-a35a92gh	156	16	the	the	DT
cord-009376-a35a92gh	156	17	quantitative	quantitative	JJ
cord-009376-a35a92gh	156	18	software	software	NN
cord-009376-a35a92gh	156	19	in	in	IN
cord-009376-a35a92gh	156	20	combination	combination	NN
cord-009376-a35a92gh	156	21	with	with	IN
cord-009376-a35a92gh	156	22	an	an	DT
cord-009376-a35a92gh	156	23	assessment	assessment	NN
cord-009376-a35a92gh	156	24	of	of	IN
cord-009376-a35a92gh	156	25	the	the	DT
cord-009376-a35a92gh	156	26	Q	Q	NNP
cord-009376-a35a92gh	156	27	-	-	HYPH
cord-009376-a35a92gh	156	28	PCR	PCR	NNP
cord-009376-a35a92gh	156	29	assay	assay	NN
cord-009376-a35a92gh	156	30	was	be	VBD
cord-009376-a35a92gh	156	31	determined	determine	VBN
cord-009376-a35a92gh	156	32	.	.	.
cord-009376-a35a92gh	157	1	The	the	DT
cord-009376-a35a92gh	157	2	results	result	NNS
cord-009376-a35a92gh	157	3	show	show	VBP
cord-009376-a35a92gh	157	4	that	that	IN
cord-009376-a35a92gh	157	5	Q	Q	NNP
cord-009376-a35a92gh	157	6	-	-	HYPH
cord-009376-a35a92gh	157	7	PCR	pcr	NN
cord-009376-a35a92gh	157	8	assays	assay	NNS
cord-009376-a35a92gh	157	9	are	be	VBP
cord-009376-a35a92gh	157	10	able	able	JJ
cord-009376-a35a92gh	157	11	to	to	TO
cord-009376-a35a92gh	157	12	quantify	quantify	VB
cord-009376-a35a92gh	157	13	accurately	accurately	RB
cord-009376-a35a92gh	157	14	the	the	DT
cord-009376-a35a92gh	157	15	correct	correct	JJ
cord-009376-a35a92gh	157	16	amount	amount	NN
cord-009376-a35a92gh	157	17	to	to	IN
cord-009376-a35a92gh	157	18	within	within	IN
cord-009376-a35a92gh	157	19	one	one	CD
cord-009376-a35a92gh	157	20	standard	standard	JJ
cord-009376-a35a92gh	157	21	deviation	deviation	NN
cord-009376-a35a92gh	157	22	for	for	IN
cord-009376-a35a92gh	157	23	both	both	CC
cord-009376-a35a92gh	157	24	DNA	dna	NN
cord-009376-a35a92gh	157	25	Ž	ž	NN
cord-009376-a35a92gh	157	26	.	.	.
cord-009376-a35a92gh	158	1	and	and	CC
cord-009376-a35a92gh	158	2	RNA	RNA	NNP
cord-009376-a35a92gh	158	3	targets	target	NNS
cord-009376-a35a92gh	158	4	Table	Table	NNP
cord-009376-a35a92gh	158	5	3	3	CD
cord-009376-a35a92gh	158	6	.	.	.
cord-009376-a35a92gh	159	1	In	in	IN
cord-009376-a35a92gh	159	2	more	more	JJR
cord-009376-a35a92gh	159	3	detail	detail	NN
cord-009376-a35a92gh	159	4	,	,	,
cord-009376-a35a92gh	159	5	the	the	DT
cord-009376-a35a92gh	159	6	Ž	ž	NN
cord-009376-a35a92gh	159	7	value	value	NN
cord-009376-a35a92gh	159	8	X	X	NNP
cord-009376-a35a92gh	159	9	s	s	POS
cord-009376-a35a92gh	159	10	1000	1000	CD
cord-009376-a35a92gh	159	11	on	on	IN
cord-009376-a35a92gh	159	12	the	the	DT
cord-009376-a35a92gh	159	13	standard	standard	JJ
cord-009376-a35a92gh	159	14	curve	curve	NN
cord-009376-a35a92gh	159	15	which	which	WDT
cord-009376-a35a92gh	159	16	is	be	VBZ
cord-009376-a35a92gh	159	17	the	the	DT
cord-009376-a35a92gh	159	18	actual	actual	JJ
cord-009376-a35a92gh	159	19	amount	amount	NN
cord-009376-a35a92gh	159	20	in	in	IN
cord-009376-a35a92gh	159	21	the	the	DT
cord-009376-a35a92gh	159	22	Enterovirus	Enterovirus	NNP
cord-009376-a35a92gh	159	23	RNA	RNA	NNP
cord-009376-a35a92gh	159	24	in	in	IN
cord-009376-a35a92gh	159	25	the	the	DT
cord-009376-a35a92gh	159	26	.	.	.
cord-009376-a35a92gh	159	27	standard	standard	NN
cord-009376-a35a92gh	159	28	,	,	,
cord-009376-a35a92gh	159	29	corresponds	correspond	VBZ
cord-009376-a35a92gh	159	30	to	to	IN
cord-009376-a35a92gh	159	31	a	a	DT
cord-009376-a35a92gh	159	32	calculated	calculate	VBN
cord-009376-a35a92gh	159	33	value	value	NN
cord-009376-a35a92gh	159	34	Y	Y	NNP
cord-009376-a35a92gh	159	35	s	s	NNP
cord-009376-a35a92gh	159	36	w	w	NN
cord-009376-a35a92gh	159	37	Ž	Ž	NNP
cord-009376-a35a92gh	159	38	994	994	CD
cord-009376-a35a92gh	159	39	"	"	``
cord-009376-a35a92gh	159	40	97	97	CD
cord-009376-a35a92gh	159	41	which	which	WDT
cord-009376-a35a92gh	159	42	is	be	VBZ
cord-009376-a35a92gh	159	43	the	the	DT
cord-009376-a35a92gh	159	44	mean	mean	JJ
cord-009376-a35a92gh	159	45	"	"	``
cord-009376-a35a92gh	159	46	standard	standard	JJ
cord-009376-a35a92gh	159	47	devia-	devia-	NN
cord-009376-a35a92gh	159	48	.	.	.
cord-009376-a35a92gh	160	1	tion	tion	NN
cord-009376-a35a92gh	160	2	amount	amount	NN
cord-009376-a35a92gh	160	3	calculated	calculate	VBN
cord-009376-a35a92gh	160	4	in	in	IN
cord-009376-a35a92gh	160	5	the	the	DT
cord-009376-a35a92gh	160	6	unknown	unknown	JJ
cord-009376-a35a92gh	160	7	replicates	replicate	NNS
cord-009376-a35a92gh	160	8	containing	contain	VBG
cord-009376-a35a92gh	160	9	identical	identical	JJ
cord-009376-a35a92gh	160	10	amounts	amount	NNS
cord-009376-a35a92gh	160	11	of	of	IN
cord-009376-a35a92gh	160	12	nucleic	nucleic	JJ
cord-009376-a35a92gh	160	13	acid	acid	NN
cord-009376-a35a92gh	160	14	x	x	NN
cord-009376-a35a92gh	160	15	as	as	IN
cord-009376-a35a92gh	160	16	the	the	DT
cord-009376-a35a92gh	160	17	standard	standard	NN
cord-009376-a35a92gh	160	18	.	.	.
cord-009376-a35a92gh	161	1	Because	because	IN
cord-009376-a35a92gh	161	2	the	the	DT
cord-009376-a35a92gh	161	3	sensitivity	sensitivity	NN
cord-009376-a35a92gh	161	4	of	of	IN
cord-009376-a35a92gh	161	5	the	the	DT
cord-009376-a35a92gh	161	6	ABI	ABI	NNP
cord-009376-a35a92gh	161	7	7700	7700	CD
cord-009376-a35a92gh	161	8	and	and	CC
cord-009376-a35a92gh	161	9	7900HT	7900ht	CD
cord-009376-a35a92gh	161	10	is	be	VBZ
cord-009376-a35a92gh	161	11	a	a	DT
cord-009376-a35a92gh	161	12	two	two	CD
cord-009376-a35a92gh	161	13	-	-	HYPH
cord-009376-a35a92gh	161	14	to	to	IN
cord-009376-a35a92gh	161	15	fourfold	fourfold	JJ
cord-009376-a35a92gh	161	16	copy	copy	NN
cord-009376-a35a92gh	161	17	number	number	NN
cord-009376-a35a92gh	161	18	difference	difference	NN
cord-009376-a35a92gh	161	19	,	,	,
cord-009376-a35a92gh	161	20	a	a	DT
cord-009376-a35a92gh	161	21	mean	mean	JJ
cord-009376-a35a92gh	161	22	value	value	NN
cord-009376-a35a92gh	161	23	of	of	IN
cord-009376-a35a92gh	161	24	Y	Y	NNP
cord-009376-a35a92gh	161	25	that	that	WDT
cord-009376-a35a92gh	161	26	varies	vary	VBZ
cord-009376-a35a92gh	161	27	by	by	IN
cord-009376-a35a92gh	161	28	more	more	JJR
cord-009376-a35a92gh	161	29	than	than	IN
cord-009376-a35a92gh	161	30	fourfold	fourfold	NN
cord-009376-a35a92gh	161	31	could	could	MD
cord-009376-a35a92gh	161	32	be	be	VB
cord-009376-a35a92gh	161	33	calculated	calculate	VBN
cord-009376-a35a92gh	161	34	as	as	IN
cord-009376-a35a92gh	161	35	being	be	VBG
cord-009376-a35a92gh	161	36	different	different	JJ
cord-009376-a35a92gh	161	37	from	from	IN
cord-009376-a35a92gh	161	38	the	the	DT
cord-009376-a35a92gh	161	39	actual	actual	JJ
cord-009376-a35a92gh	161	40	value	value	NN
cord-009376-a35a92gh	161	41	of	of	IN
cord-009376-a35a92gh	161	42	Y	Y	NNP
cord-009376-a35a92gh	161	43	,	,	,
cord-009376-a35a92gh	161	44	if	if	IN
cord-009376-a35a92gh	161	45	working	work	VBG
cord-009376-a35a92gh	161	46	at	at	IN
cord-009376-a35a92gh	161	47	the	the	DT
cord-009376-a35a92gh	161	48	two	two	CD
cord-009376-a35a92gh	161	49	-	-	HYPH
cord-009376-a35a92gh	161	50	to	to	IN
cord-009376-a35a92gh	161	51	fourfold	fourfold	JJ
cord-009376-a35a92gh	161	52	copy	copy	NN
cord-009376-a35a92gh	161	53	number	number	NN
cord-009376-a35a92gh	161	54	difference	difference	NN
cord-009376-a35a92gh	161	55	level	level	NN
cord-009376-a35a92gh	161	56	.	.	.
cord-009376-a35a92gh	162	1	Therefore	therefore	RB
cord-009376-a35a92gh	162	2	,	,	,
cord-009376-a35a92gh	162	3	for	for	IN
cord-009376-a35a92gh	162	4	an	an	DT
cord-009376-a35a92gh	162	5	actual	actual	JJ
cord-009376-a35a92gh	162	6	value	value	NN
cord-009376-a35a92gh	162	7	Ž	ž	NN
cord-009376-a35a92gh	162	8	.	.	.
cord-009376-a35a92gh	163	1	X	X	NNS
cord-009376-a35a92gh	163	2	of	of	IN
cord-009376-a35a92gh	163	3	1000	1000	CD
cord-009376-a35a92gh	163	4	copies	copy	NNS
cord-009376-a35a92gh	163	5	the	the	DT
cord-009376-a35a92gh	163	6	acceptable	acceptable	JJ
cord-009376-a35a92gh	163	7	criteria	criterion	NNS
cord-009376-a35a92gh	163	8	could	could	MD
cord-009376-a35a92gh	163	9	be	be	VB
cord-009376-a35a92gh	163	10	a	a	DT
cord-009376-a35a92gh	163	11	one	one	CD
cord-009376-a35a92gh	163	12	-	-	HYPH
cord-009376-a35a92gh	163	13	to	to	IN
cord-009376-a35a92gh	163	14	twofold	twofold	JJ
cord-009376-a35a92gh	163	15	variation	variation	NN
cord-009376-a35a92gh	163	16	on	on	IN
cord-009376-a35a92gh	163	17	either	either	DT
cord-009376-a35a92gh	163	18	side	side	NN
cord-009376-a35a92gh	163	19	of	of	IN
cord-009376-a35a92gh	163	20	Ž	ž	NN
cord-009376-a35a92gh	164	1	the	the	DT
cord-009376-a35a92gh	164	2	1000	1000	CD
cord-009376-a35a92gh	164	3	copies	copy	NNS
cord-009376-a35a92gh	164	4	e.g.	e.g.	RB
cord-009376-a35a92gh	164	5	from	from	IN
cord-009376-a35a92gh	164	6	500	500	CD
cord-009376-a35a92gh	164	7	᎐	᎐	CD
cord-009376-a35a92gh	164	8	750	750	CD
cord-009376-a35a92gh	164	9	to	to	IN
cord-009376-a35a92gh	164	10	1500	1500	CD
cord-009376-a35a92gh	164	11	᎐	᎐	CD
cord-009376-a35a92gh	164	12	2000	2000	CD
cord-009376-a35a92gh	164	13	.	.	.
cord-009376-a35a92gh	165	1	copies	copy	NNS
cord-009376-a35a92gh	165	2	;	;	:
cord-009376-a35a92gh	165	3	a	a	DT
cord-009376-a35a92gh	165	4	total	total	NN
cord-009376-a35a92gh	165	5	of	of	IN
cord-009376-a35a92gh	165	6	two	two	CD
cord-009376-a35a92gh	165	7	-	-	HYPH
cord-009376-a35a92gh	165	8	to	to	IN
cord-009376-a35a92gh	165	9	fourfold	fourfold	JJ
cord-009376-a35a92gh	165	10	variation	variation	NN
cord-009376-a35a92gh	165	11	of	of	IN
cord-009376-a35a92gh	165	12	the	the	DT
cord-009376-a35a92gh	165	13	Ž	ž	NN
cord-009376-a35a92gh	165	14	.	.	.
cord-009376-a35a92gh	166	1	actual	actual	JJ
cord-009376-a35a92gh	166	2	value	value	NN
cord-009376-a35a92gh	167	1	X	X	NNS
cord-009376-a35a92gh	167	2	.	.	.
cord-009376-a35a92gh	168	1	Therefore	therefore	RB
cord-009376-a35a92gh	168	2	,	,	,
cord-009376-a35a92gh	168	3	the	the	DT
cord-009376-a35a92gh	168	4	value	value	NN
cord-009376-a35a92gh	168	5	of	of	IN
cord-009376-a35a92gh	168	6	Y	Y	NNP
cord-009376-a35a92gh	168	7	s	s	NNP
cord-009376-a35a92gh	168	8	994	994	CD
cord-009376-a35a92gh	168	9	"	"	``
cord-009376-a35a92gh	168	10	97	97	CD
cord-009376-a35a92gh	168	11	would	would	MD
cord-009376-a35a92gh	168	12	be	be	VB
cord-009376-a35a92gh	168	13	within	within	IN
cord-009376-a35a92gh	168	14	the	the	DT
cord-009376-a35a92gh	168	15	acceptable	acceptable	JJ
cord-009376-a35a92gh	168	16	criteria	criterion	NNS
cord-009376-a35a92gh	168	17	.	.	.
cord-009376-a35a92gh	169	1	Any	any	DT
cord-009376-a35a92gh	169	2	value	value	NN
cord-009376-a35a92gh	169	3	outside	outside	IN
cord-009376-a35a92gh	169	4	of	of	IN
cord-009376-a35a92gh	169	5	the	the	DT
cord-009376-a35a92gh	169	6	acceptable	acceptable	JJ
cord-009376-a35a92gh	169	7	limit	limit	NN
cord-009376-a35a92gh	169	8	could	could	MD
cord-009376-a35a92gh	169	9	be	be	VB
cord-009376-a35a92gh	169	10	considered	consider	VBN
cord-009376-a35a92gh	169	11	as	as	RB
cord-009376-a35a92gh	169	12	too	too	RB
cord-009376-a35a92gh	169	13	highly	highly	RB
cord-009376-a35a92gh	169	14	variable	variable	JJ
cord-009376-a35a92gh	169	15	for	for	IN
cord-009376-a35a92gh	169	16	accurate	accurate	JJ
cord-009376-a35a92gh	169	17	quantification	quantification	NN
cord-009376-a35a92gh	169	18	.	.	.
cord-009376-a35a92gh	170	1	This	this	DT
cord-009376-a35a92gh	170	2	approach	approach	NN
cord-009376-a35a92gh	170	3	may	may	MD
cord-009376-a35a92gh	170	4	allow	allow	VB
cord-009376-a35a92gh	170	5	one	one	NN
cord-009376-a35a92gh	170	6	to	to	IN
cord-009376-a35a92gh	170	7	Ž	Ž	NNP
cord-009376-a35a92gh	170	8	.	.	.
cord-009376-a35a92gh	171	1	Actual	actual	JJ
cord-009376-a35a92gh	171	2	values	value	NNS
cord-009376-a35a92gh	172	1	X	X	NNS
cord-009376-a35a92gh	172	2	are	be	VBP
cord-009376-a35a92gh	172	3	the	the	DT
cord-009376-a35a92gh	172	4	known	know	VBN
cord-009376-a35a92gh	172	5	amount	amount	NN
cord-009376-a35a92gh	172	6	in	in	IN
cord-009376-a35a92gh	172	7	the	the	DT
cord-009376-a35a92gh	172	8	standard	standard	JJ
cord-009376-a35a92gh	172	9	Ž	Ž	NNP
cord-009376-a35a92gh	172	10	.	.	.
cord-009376-a35a92gh	172	11	reaction	reaction	NN
cord-009376-a35a92gh	172	12	.	.	.
cord-009376-a35a92gh	173	1	Values	Values	NNP
cord-009376-a35a92gh	173	2	Y	Y	NNP
cord-009376-a35a92gh	173	3	are	be	VBP
cord-009376-a35a92gh	173	4	the	the	DT
cord-009376-a35a92gh	173	5	amounts	amount	NNS
cord-009376-a35a92gh	173	6	calculated	calculate	VBN
cord-009376-a35a92gh	173	7	in	in	IN
cord-009376-a35a92gh	173	8	the	the	DT
cord-009376-a35a92gh	173	9	'	'	``
cord-009376-a35a92gh	173	10	standardised	standardised	JJ
cord-009376-a35a92gh	173	11	unknowns	unknown	NNS
cord-009376-a35a92gh	173	12	'	'	''
cord-009376-a35a92gh	173	13	from	from	IN
cord-009376-a35a92gh	173	14	the	the	DT
cord-009376-a35a92gh	173	15	generated	generate	VBN
cord-009376-a35a92gh	173	16	standard	standard	JJ
cord-009376-a35a92gh	173	17	curve	curve	NN
cord-009376-a35a92gh	173	18	,	,	,
cord-009376-a35a92gh	173	19	and	and	CC
cord-009376-a35a92gh	173	20	are	be	VBP
cord-009376-a35a92gh	173	21	expressed	express	VBN
cord-009376-a35a92gh	173	22	as	as	IN
cord-009376-a35a92gh	173	23	mean	mean	JJ
cord-009376-a35a92gh	173	24	copy	copy	NN
cord-009376-a35a92gh	173	25	number	number	NN
cord-009376-a35a92gh	173	26	"	"	''
cord-009376-a35a92gh	173	27	S.D.	S.D.	NNP
cord-009376-a35a92gh	173	28	assess	assess	VBP
cord-009376-a35a92gh	173	29	the	the	DT
cord-009376-a35a92gh	173	30	performance	performance	NN
cord-009376-a35a92gh	173	31	of	of	IN
cord-009376-a35a92gh	173	32	the	the	DT
cord-009376-a35a92gh	173	33	standards	standard	NNS
cord-009376-a35a92gh	173	34	and	and	CC
cord-009376-a35a92gh	173	35	the	the	DT
cord-009376-a35a92gh	173	36	overall	overall	JJ
cord-009376-a35a92gh	173	37	Q	Q	NNP
cord-009376-a35a92gh	173	38	-	-	HYPH
cord-009376-a35a92gh	173	39	PCR	pcr	NN
cord-009376-a35a92gh	173	40	assay	assay	NN
cord-009376-a35a92gh	173	41	in	in	IN
cord-009376-a35a92gh	173	42	terms	term	NNS
cord-009376-a35a92gh	173	43	of	of	IN
cord-009376-a35a92gh	173	44	quantification	quantification	NN
cord-009376-a35a92gh	173	45	accuracy	accuracy	NN
cord-009376-a35a92gh	173	46	.	.	.
cord-009376-a35a92gh	174	1	Adventitious	adventitious	JJ
cord-009376-a35a92gh	174	2	agents	agent	NNS
cord-009376-a35a92gh	174	3	pose	pose	VBP
cord-009376-a35a92gh	174	4	significant	significant	JJ
cord-009376-a35a92gh	174	5	risk	risk	NN
cord-009376-a35a92gh	174	6	if	if	IN
cord-009376-a35a92gh	174	7	present	present	JJ
cord-009376-a35a92gh	174	8	in	in	IN
cord-009376-a35a92gh	174	9	vaccines	vaccine	NNS
cord-009376-a35a92gh	174	10	and	and	CC
cord-009376-a35a92gh	174	11	therapeutics	therapeutic	NNS
cord-009376-a35a92gh	174	12	.	.	.
cord-009376-a35a92gh	175	1	Therefore	therefore	RB
cord-009376-a35a92gh	175	2	,	,	,
cord-009376-a35a92gh	175	3	highly	highly	RB
cord-009376-a35a92gh	175	4	sensitive	sensitive	JJ
cord-009376-a35a92gh	175	5	PCR	pcr	NN
cord-009376-a35a92gh	175	6	assays	assay	NNS
cord-009376-a35a92gh	175	7	for	for	IN
cord-009376-a35a92gh	175	8	the	the	DT
cord-009376-a35a92gh	175	9	detection	detection	NN
cord-009376-a35a92gh	175	10	of	of	IN
cord-009376-a35a92gh	175	11	these	these	DT
cord-009376-a35a92gh	175	12	agents	agent	NNS
cord-009376-a35a92gh	175	13	are	be	VBP
cord-009376-a35a92gh	175	14	crucial	crucial	JJ
cord-009376-a35a92gh	175	15	for	for	IN
cord-009376-a35a92gh	175	16	safety	safety	NN
cord-009376-a35a92gh	175	17	testing	testing	NN
cord-009376-a35a92gh	175	18	.	.	.
cord-009376-a35a92gh	176	1	Numerous	numerous	JJ
cord-009376-a35a92gh	176	2	viral	viral	JJ
cord-009376-a35a92gh	176	3	nucleic	nucleic	JJ
cord-009376-a35a92gh	176	4	acid	acid	NN
cord-009376-a35a92gh	176	5	detection	detection	NN
cord-009376-a35a92gh	176	6	assays	assay	NNS
cord-009376-a35a92gh	176	7	are	be	VBP
cord-009376-a35a92gh	176	8	available	available	JJ
cord-009376-a35a92gh	176	9	,	,	,
cord-009376-a35a92gh	176	10	and	and	CC
cord-009376-a35a92gh	176	11	the	the	DT
cord-009376-a35a92gh	176	12	available	available	JJ
cord-009376-a35a92gh	176	13	number	number	NN
cord-009376-a35a92gh	176	14	of	of	IN
cord-009376-a35a92gh	176	15	fully	fully	RB
cord-009376-a35a92gh	176	16	validated	validate	VBN
cord-009376-a35a92gh	176	17	Q	Q	NNP
cord-009376-a35a92gh	176	18	-	-	HYPH
cord-009376-a35a92gh	176	19	PCR	PCR	NNP
cord-009376-a35a92gh	176	20	pathogen	pathogen	NN
cord-009376-a35a92gh	176	21	detection	detection	NN
cord-009376-a35a92gh	176	22	assays	assay	NNS
cord-009376-a35a92gh	176	23	is	be	VBZ
cord-009376-a35a92gh	176	24	increasing	increase	VBG
cord-009376-a35a92gh	176	25	at	at	IN
cord-009376-a35a92gh	176	26	a	a	DT
cord-009376-a35a92gh	176	27	considerable	considerable	JJ
cord-009376-a35a92gh	176	28	rate	rate	NN
cord-009376-a35a92gh	176	29	.	.	.
cord-009376-a35a92gh	177	1	Besides	besides	IN
cord-009376-a35a92gh	177	2	viral	viral	JJ
cord-009376-a35a92gh	177	3	-	-	HYPH
cord-009376-a35a92gh	177	4	specific	specific	JJ
cord-009376-a35a92gh	177	5	nucleic	nucleic	JJ
cord-009376-a35a92gh	177	6	acid	acid	NN
cord-009376-a35a92gh	177	7	detection	detection	NN
cord-009376-a35a92gh	177	8	,	,	,
cord-009376-a35a92gh	177	9	one	one	CD
cord-009376-a35a92gh	177	10	very	very	RB
cord-009376-a35a92gh	177	11	important	important	JJ
cord-009376-a35a92gh	177	12	use	use	NN
cord-009376-a35a92gh	177	13	of	of	IN
cord-009376-a35a92gh	177	14	Q	Q	NNP
cord-009376-a35a92gh	177	15	-	-	HYPH
cord-009376-a35a92gh	177	16	PCR	PCR	NNP
cord-009376-a35a92gh	177	17	technology	technology	NN
cord-009376-a35a92gh	177	18	in	in	IN
cord-009376-a35a92gh	177	19	safety	safety	NN
cord-009376-a35a92gh	177	20	testing	testing	NN
cord-009376-a35a92gh	177	21	is	be	VBZ
cord-009376-a35a92gh	177	22	the	the	DT
cord-009376-a35a92gh	177	23	detection	detection	NN
cord-009376-a35a92gh	177	24	of	of	IN
cord-009376-a35a92gh	177	25	contaminating	contaminate	VBG
cord-009376-a35a92gh	177	26	retroviruses	retrovirus	NNS
cord-009376-a35a92gh	177	27	by	by	IN
cord-009376-a35a92gh	177	28	the	the	DT
cord-009376-a35a92gh	177	29	identification	identification	NN
cord-009376-a35a92gh	177	30	of	of	IN
cord-009376-a35a92gh	177	31	the	the	DT
cord-009376-a35a92gh	177	32	presence	presence	NN
cord-009376-a35a92gh	177	33	of	of	IN
cord-009376-a35a92gh	177	34	retroviral	retroviral	JJ
cord-009376-a35a92gh	177	35	reverse	reverse	NN
cord-009376-a35a92gh	177	36	transcriptase	transcriptase	NN
cord-009376-a35a92gh	177	37	Ž	ž	NN
cord-009376-a35a92gh	177	38	.	.	.
cord-009376-a35a92gh	178	1	activity	activity	NN
cord-009376-a35a92gh	179	1	Lovatt	Lovatt	NNP
cord-009376-a35a92gh	179	2	et	et	NNP
cord-009376-a35a92gh	179	3	al	al	NNP
cord-009376-a35a92gh	179	4	.	.	NNP
cord-009376-a35a92gh	179	5	,	,	,
cord-009376-a35a92gh	179	6	1999	1999	CD
cord-009376-a35a92gh	179	7	.	.	.
cord-009376-a35a92gh	180	1	The	the	DT
cord-009376-a35a92gh	180	2	fluorescent	fluorescent	JJ
cord-009376-a35a92gh	180	3	product	product	NN
cord-009376-a35a92gh	180	4	enhanced	enhance	VBN
cord-009376-a35a92gh	180	5	reverse	reverse	NN
cord-009376-a35a92gh	180	6	tran	tran	NNP
cord-009376-a35a92gh	180	7	-	-	HYPH
cord-009376-a35a92gh	180	8	Ž	Ž	NNP
cord-009376-a35a92gh	180	9	.	.	.
cord-009376-a35a92gh	181	1	scriptase	scriptase	NNP
cord-009376-a35a92gh	181	2	F	F	NNP
cord-009376-a35a92gh	181	3	-	-	HYPH
cord-009376-a35a92gh	181	4	PERT	pert	JJ
cord-009376-a35a92gh	181	5	assay	assay	NN
cord-009376-a35a92gh	181	6	,	,	,
cord-009376-a35a92gh	181	7	is	be	VBZ
cord-009376-a35a92gh	181	8	an	an	DT
cord-009376-a35a92gh	181	9	extremely	extremely	RB
cord-009376-a35a92gh	181	10	sensitive	sensitive	JJ
cord-009376-a35a92gh	181	11	tests	test	NNS
cord-009376-a35a92gh	181	12	for	for	IN
cord-009376-a35a92gh	181	13	the	the	DT
cord-009376-a35a92gh	181	14	detection	detection	NN
cord-009376-a35a92gh	181	15	of	of	IN
cord-009376-a35a92gh	181	16	reverse	reverse	JJ
cord-009376-a35a92gh	181	17	transcrip	transcrip	NN
cord-009376-a35a92gh	181	18	-	-	HYPH
cord-009376-a35a92gh	181	19	Ž	Ž	NNP
cord-009376-a35a92gh	181	20	.	.	.
cord-009376-a35a92gh	182	1	5	5	CD
cord-009376-a35a92gh	182	2	tase	tase	NNP
cord-009376-a35a92gh	182	3	RT	RT	NNP
cord-009376-a35a92gh	182	4	.	.	.
cord-009376-a35a92gh	183	1	F	F	NNP
cord-009376-a35a92gh	183	2	-	-	HYPH
cord-009376-a35a92gh	183	3	PERT	PERT	NNP
cord-009376-a35a92gh	183	4	is	be	VBZ
cord-009376-a35a92gh	183	5	up	up	IN
cord-009376-a35a92gh	183	6	to	to	TO
cord-009376-a35a92gh	183	7	10	10	CD
cord-009376-a35a92gh	183	8	-fold	-fold	NN
cord-009376-a35a92gh	183	9	more	more	RBR
cord-009376-a35a92gh	183	10	sensitive	sensitive	JJ
cord-009376-a35a92gh	183	11	than	than	IN
cord-009376-a35a92gh	183	12	conventional	conventional	JJ
cord-009376-a35a92gh	183	13	RT	RT	NNP
cord-009376-a35a92gh	183	14	assays	assay	NNS
cord-009376-a35a92gh	183	15	for	for	IN
cord-009376-a35a92gh	183	16	detecting	detect	VBG
cord-009376-a35a92gh	183	17	the	the	DT
cord-009376-a35a92gh	183	18	Ž	ž	NN
cord-009376-a35a92gh	183	19	presence	presence	NN
cord-009376-a35a92gh	183	20	of	of	IN
cord-009376-a35a92gh	183	21	retroviruses	retrovirus	NNS
cord-009376-a35a92gh	183	22	Maudru	Maudru	NNP
cord-009376-a35a92gh	183	23	and	and	CC
cord-009376-a35a92gh	183	24	Peden	Peden	NNP
cord-009376-a35a92gh	183	25	,	,	,
cord-009376-a35a92gh	183	26	.	.	.
cord-009376-a35a92gh	184	1	1997	1997	CD
cord-009376-a35a92gh	184	2	;	;	:
cord-009376-a35a92gh	184	3	Lovatt	Lovatt	NNP
cord-009376-a35a92gh	184	4	et	et	NNP
cord-009376-a35a92gh	184	5	al	al	NNP
cord-009376-a35a92gh	184	6	.	.	NNP
cord-009376-a35a92gh	184	7	,	,	,
cord-009376-a35a92gh	184	8	1999	1999	CD
cord-009376-a35a92gh	184	9	.	.	.
cord-009376-a35a92gh	185	1	This	this	DT
cord-009376-a35a92gh	185	2	increased	increase	VBN
cord-009376-a35a92gh	185	3	sensitivity	sensitivity	NN
cord-009376-a35a92gh	185	4	,	,	,
cord-009376-a35a92gh	185	5	prompted	prompt	VBD
cord-009376-a35a92gh	185	6	The	the	DT
cord-009376-a35a92gh	185	7	Center	Center	NNP
cord-009376-a35a92gh	185	8	for	for	IN
cord-009376-a35a92gh	185	9	Biologics	Biologics	NNP
cord-009376-a35a92gh	185	10	Evalua	Evalua	NNP
cord-009376-a35a92gh	185	11	-	-	HYPH
cord-009376-a35a92gh	185	12	Ž	Ž	NNP
cord-009376-a35a92gh	185	13	.	.	.
cord-009376-a35a92gh	185	14	tion	tion	NN
cord-009376-a35a92gh	185	15	and	and	CC
cord-009376-a35a92gh	185	16	Research	Research	NNP
cord-009376-a35a92gh	185	17	CBER	CBER	NNP
cord-009376-a35a92gh	185	18	to	to	TO
cord-009376-a35a92gh	185	19	request	request	VB
cord-009376-a35a92gh	185	20	PERT	pert	JJ
cord-009376-a35a92gh	185	21	testing	testing	NN
cord-009376-a35a92gh	185	22	on	on	IN
cord-009376-a35a92gh	185	23	all	all	DT
cord-009376-a35a92gh	185	24	viral	viral	JJ
cord-009376-a35a92gh	185	25	vaccines	vaccine	NNS
cord-009376-a35a92gh	185	26	being	be	VBG
cord-009376-a35a92gh	185	27	investigated	investigate	VBN
cord-009376-a35a92gh	185	28	for	for	IN
cord-009376-a35a92gh	185	29	(	(	-LRB-
cord-009376-a35a92gh	185	30	)	)	-RRB-
cord-009376-a35a92gh	185	31	humans	human	NNS
cord-009376-a35a92gh	185	32	.	.	.
cord-009376-a35a92gh	186	1	Consequently	consequently	RB
cord-009376-a35a92gh	186	2	,	,	,
cord-009376-a35a92gh	186	3	F	F	NNP
cord-009376-a35a92gh	186	4	-	-	HYPH
cord-009376-a35a92gh	186	5	PERT	PERT	NNP
cord-009376-a35a92gh	186	6	is	be	VBZ
cord-009376-a35a92gh	186	7	the	the	DT
cord-009376-a35a92gh	186	8	first	first	JJ
cord-009376-a35a92gh	186	9	choice	choice	NN
cord-009376-a35a92gh	186	10	for	for	IN
cord-009376-a35a92gh	186	11	detection	detection	NN
cord-009376-a35a92gh	186	12	of	of	IN
cord-009376-a35a92gh	186	13	RT	RT	NNP
cord-009376-a35a92gh	186	14	in	in	IN
cord-009376-a35a92gh	186	15	live	live	JJ
cord-009376-a35a92gh	186	16	viral	viral	JJ
cord-009376-a35a92gh	186	17	vaccines	vaccine	NNS
cord-009376-a35a92gh	186	18	,	,	,
cord-009376-a35a92gh	186	19	gene	gene	NN
cord-009376-a35a92gh	186	20	therapy	therapy	NN
cord-009376-a35a92gh	186	21	preparations	preparation	NNS
cord-009376-a35a92gh	186	22	and	and	CC
cord-009376-a35a92gh	186	23	the	the	DT
cord-009376-a35a92gh	186	24	screening	screening	NN
cord-009376-a35a92gh	186	25	of	of	IN
cord-009376-a35a92gh	186	26	animals	animal	NNS
cord-009376-a35a92gh	186	27	and	and	CC
cord-009376-a35a92gh	186	28	patients	patient	NNS
cord-009376-a35a92gh	186	29	for	for	IN
cord-009376-a35a92gh	186	30	Porcine	porcine	JJ
cord-009376-a35a92gh	186	31	endogenous	endogenous	JJ
cord-009376-a35a92gh	186	32	retro	retro	JJ
cord-009376-a35a92gh	186	33	-	-	HYPH
cord-009376-a35a92gh	186	34	Ž	ž	NN
cord-009376-a35a92gh	186	35	.	.	.
cord-009376-a35a92gh	187	1	virus	virus	NN
cord-009376-a35a92gh	187	2	PERV	PERV	NNP
cord-009376-a35a92gh	187	3	in	in	IN
cord-009376-a35a92gh	187	4	xenotransplantation	xenotransplantation	NN
cord-009376-a35a92gh	187	5	trials	trial	NNS
cord-009376-a35a92gh	187	6	.	.	.
cord-009376-a35a92gh	188	1	F	F	NNP
cord-009376-a35a92gh	188	2	-	-	HYPH
cord-009376-a35a92gh	188	3	PERT	PERT	NNP
cord-009376-a35a92gh	188	4	is	be	VBZ
cord-009376-a35a92gh	188	5	a	a	DT
cord-009376-a35a92gh	188	6	RT	rt	NN
cord-009376-a35a92gh	188	7	-	-	HYPH
cord-009376-a35a92gh	188	8	dependent	dependent	JJ
cord-009376-a35a92gh	188	9	Q	Q	NNP
cord-009376-a35a92gh	188	10	-	-	HYPH
cord-009376-a35a92gh	188	11	PCR	pcr	NN
cord-009376-a35a92gh	188	12	assay	assay	NN
cord-009376-a35a92gh	188	13	and	and	CC
cord-009376-a35a92gh	188	14	therefore	therefore	RB
cord-009376-a35a92gh	188	15	combines	combine	VBZ
cord-009376-a35a92gh	188	16	the	the	DT
cord-009376-a35a92gh	188	17	broad	broad	JJ
cord-009376-a35a92gh	188	18	specificity	specificity	NN
cord-009376-a35a92gh	188	19	of	of	IN
cord-009376-a35a92gh	188	20	conventional	conventional	JJ
cord-009376-a35a92gh	188	21	RT	rt	NN
cord-009376-a35a92gh	188	22	assays	assay	NNS
cord-009376-a35a92gh	188	23	with	with	IN
cord-009376-a35a92gh	188	24	the	the	DT
cord-009376-a35a92gh	188	25	high	high	JJ
cord-009376-a35a92gh	188	26	sensitivity	sensitivity	NN
cord-009376-a35a92gh	188	27	and	and	CC
cord-009376-a35a92gh	188	28	fast	fast	JJ
cord-009376-a35a92gh	188	29	throughput	throughput	NN
cord-009376-a35a92gh	188	30	of	of	IN
cord-009376-a35a92gh	188	31	Q	Q	NNP
cord-009376-a35a92gh	188	32	-	-	HYPH
cord-009376-a35a92gh	188	33	PCR	PCR	NNP
cord-009376-a35a92gh	188	34	.	.	.
cord-009376-a35a92gh	189	1	As	as	IN
cord-009376-a35a92gh	189	2	with	with	IN
cord-009376-a35a92gh	189	3	conventional	conventional	JJ
cord-009376-a35a92gh	189	4	RT	RT	NNP
cord-009376-a35a92gh	189	5	assays	assay	NNS
cord-009376-a35a92gh	189	6	,	,	,
cord-009376-a35a92gh	189	7	F	F	NNP
cord-009376-a35a92gh	189	8	-	-	HYPH
cord-009376-a35a92gh	189	9	PERT	PERT	NNP
cord-009376-a35a92gh	189	10	is	be	VBZ
cord-009376-a35a92gh	189	11	utilised	utilise	VBN
cord-009376-a35a92gh	189	12	to	to	TO
cord-009376-a35a92gh	189	13	detect	detect	VB
cord-009376-a35a92gh	189	14	the	the	DT
cord-009376-a35a92gh	189	15	RT	RT	NNP
cord-009376-a35a92gh	189	16	activity	activity	NN
cord-009376-a35a92gh	189	17	packaged	package	VBN
cord-009376-a35a92gh	189	18	into	into	IN
cord-009376-a35a92gh	189	19	extracellular	extracellular	JJ
cord-009376-a35a92gh	189	20	retrovirus	retrovirus	NN
cord-009376-a35a92gh	189	21	particles	particle	NNS
cord-009376-a35a92gh	189	22	.	.	.
cord-009376-a35a92gh	190	1	The	the	DT
cord-009376-a35a92gh	190	2	assay	assay	NN
cord-009376-a35a92gh	190	3	involves	involve	VBZ
cord-009376-a35a92gh	190	4	converting	convert	VBG
cord-009376-a35a92gh	190	5	RNA	RNA	NNP
cord-009376-a35a92gh	190	6	template	template	NN
cord-009376-a35a92gh	190	7	to	to	TO
cord-009376-a35a92gh	190	8	cDNA	cdna	VB
cord-009376-a35a92gh	190	9	and	and	CC
cord-009376-a35a92gh	190	10	then	then	RB
cord-009376-a35a92gh	190	11	amplifying	amplify	VBG
cord-009376-a35a92gh	190	12	the	the	DT
cord-009376-a35a92gh	190	13	cDNA	cdna	NN
cord-009376-a35a92gh	190	14	using	use	VBG
cord-009376-a35a92gh	190	15	product	product	NN
cord-009376-a35a92gh	190	16	-	-	HYPH
cord-009376-a35a92gh	190	17	specific	specific	JJ
cord-009376-a35a92gh	190	18	primers	primer	NNS
cord-009376-a35a92gh	190	19	.	.	.
cord-009376-a35a92gh	191	1	As	as	IN
cord-009376-a35a92gh	191	2	no	no	DT
cord-009376-a35a92gh	191	3	exogenous	exogenous	JJ
cord-009376-a35a92gh	191	4	RT	rt	NN
cord-009376-a35a92gh	191	5	activity	activity	NN
cord-009376-a35a92gh	191	6	is	be	VBZ
cord-009376-a35a92gh	191	7	added	add	VBN
cord-009376-a35a92gh	191	8	to	to	IN
cord-009376-a35a92gh	191	9	the	the	DT
cord-009376-a35a92gh	191	10	reaction	reaction	NN
cord-009376-a35a92gh	191	11	,	,	,
cord-009376-a35a92gh	191	12	cDNA	cdna	NN
cord-009376-a35a92gh	191	13	will	will	MD
cord-009376-a35a92gh	191	14	only	only	RB
cord-009376-a35a92gh	191	15	be	be	VB
cord-009376-a35a92gh	191	16	generated	generate	VBN
cord-009376-a35a92gh	191	17	if	if	IN
cord-009376-a35a92gh	191	18	the	the	DT
cord-009376-a35a92gh	191	19	sample	sample	NN
cord-009376-a35a92gh	191	20	itself	-PRON-	PRP
cord-009376-a35a92gh	191	21	contains	contain	VBZ
cord-009376-a35a92gh	191	22	RT	RT	NNP
cord-009376-a35a92gh	191	23	activity	activity	NN
cord-009376-a35a92gh	191	24	.	.	.
cord-009376-a35a92gh	192	1	When	when	WRB
cord-009376-a35a92gh	192	2	RT	RT	NNP
cord-009376-a35a92gh	192	3	activity	activity	NN
cord-009376-a35a92gh	192	4	is	be	VBZ
cord-009376-a35a92gh	192	5	not	not	RB
cord-009376-a35a92gh	192	6	present	present	JJ
cord-009376-a35a92gh	192	7	,	,	,
cord-009376-a35a92gh	192	8	the	the	DT
cord-009376-a35a92gh	192	9	Ž	ž	NN
cord-009376-a35a92gh	192	10	product	product	NN
cord-009376-a35a92gh	192	11	will	will	MD
cord-009376-a35a92gh	192	12	not	not	RB
cord-009376-a35a92gh	192	13	be	be	VB
cord-009376-a35a92gh	192	14	detected	detect	VBN
cord-009376-a35a92gh	193	1	Silver	silver	NN
cord-009376-a35a92gh	193	2	et	et	NNP
cord-009376-a35a92gh	193	3	al	al	NNP
cord-009376-a35a92gh	193	4	.	.	.
cord-009376-a35a92gh	194	1	,	,	,
cord-009376-a35a92gh	194	2	1993	1993	CD
cord-009376-a35a92gh	194	3	;	;	:
cord-009376-a35a92gh	194	4	Pyra	Pyra	NNP
cord-009376-a35a92gh	194	5	et	et	FW
cord-009376-a35a92gh	194	6	al	al	NNP
cord-009376-a35a92gh	194	7	.	.	.
cord-009376-a35a92gh	195	1	,	,	,
cord-009376-a35a92gh	195	2	1994	1994	CD
cord-009376-a35a92gh	195	3	;	;	:
cord-009376-a35a92gh	195	4	Heneine	Heneine	NNP
cord-009376-a35a92gh	195	5	et	et	FW
cord-009376-a35a92gh	195	6	al	al	NNP
cord-009376-a35a92gh	195	7	.	.	NNP
cord-009376-a35a92gh	195	8	,	,	,
cord-009376-a35a92gh	195	9	1995	1995	CD
cord-009376-a35a92gh	195	10	;	;	:
cord-009376-a35a92gh	195	11	Arnold	Arnold	NNP
cord-009376-a35a92gh	195	12	et	et	NNP
cord-009376-a35a92gh	195	13	.	.	.
cord-009376-a35a92gh	196	1	al	al	NNP
cord-009376-a35a92gh	196	2	.	.	NNP
cord-009376-a35a92gh	196	3	,	,	,
cord-009376-a35a92gh	196	4	1998	1998	CD
cord-009376-a35a92gh	196	5	.	.	.
cord-009376-a35a92gh	197	1	One	one	CD
cord-009376-a35a92gh	197	2	major	major	JJ
cord-009376-a35a92gh	197	3	advantage	advantage	NN
cord-009376-a35a92gh	197	4	of	of	IN
cord-009376-a35a92gh	197	5	F	F	NNP
cord-009376-a35a92gh	197	6	-	-	HYPH
cord-009376-a35a92gh	197	7	PERT	pert	JJ
cord-009376-a35a92gh	197	8	assays	assay	NNS
cord-009376-a35a92gh	197	9	over	over	IN
cord-009376-a35a92gh	197	10	other	other	JJ
cord-009376-a35a92gh	197	11	similar	similar	JJ
cord-009376-a35a92gh	197	12	types	type	NNS
cord-009376-a35a92gh	197	13	of	of	IN
cord-009376-a35a92gh	197	14	assays	assay	NNS
cord-009376-a35a92gh	197	15	,	,	,
cord-009376-a35a92gh	197	16	is	be	VBZ
cord-009376-a35a92gh	197	17	the	the	DT
cord-009376-a35a92gh	197	18	capability	capability	NN
cord-009376-a35a92gh	197	19	to	to	TO
cord-009376-a35a92gh	197	20	discriminate	discriminate	VB
cord-009376-a35a92gh	197	21	between	between	IN
cord-009376-a35a92gh	197	22	the	the	DT
cord-009376-a35a92gh	197	23	amplification	amplification	NN
cord-009376-a35a92gh	197	24	signals	signal	NNS
cord-009376-a35a92gh	197	25	generated	generate	VBN
cord-009376-a35a92gh	197	26	by	by	IN
cord-009376-a35a92gh	197	27	retroviral	retroviral	JJ
cord-009376-a35a92gh	197	28	RT	rt	NN
cord-009376-a35a92gh	197	29	activity	activity	NN
cord-009376-a35a92gh	197	30	and	and	CC
cord-009376-a35a92gh	197	31	those	those	DT
cord-009376-a35a92gh	197	32	which	which	WDT
cord-009376-a35a92gh	197	33	are	be	VBP
cord-009376-a35a92gh	197	34	a	a	DT
cord-009376-a35a92gh	197	35	result	result	NN
cord-009376-a35a92gh	197	36	of	of	IN
cord-009376-a35a92gh	197	37	the	the	DT
cord-009376-a35a92gh	197	38	RT	rt	NN
cord-009376-a35a92gh	197	39	-	-	HYPH
cord-009376-a35a92gh	197	40	like	like	JJ
cord-009376-a35a92gh	197	41	activity	activity	NN
cord-009376-a35a92gh	197	42	from	from	IN
cord-009376-a35a92gh	197	43	DNA	dna	NN
cord-009376-a35a92gh	197	44	polymerases	polymerase	NNS
cord-009376-a35a92gh	197	45	.	.	.
cord-009376-a35a92gh	198	1	F	F	NNP
cord-009376-a35a92gh	198	2	-	-	HYPH
cord-009376-a35a92gh	198	3	PERT	PERT	NNP
cord-009376-a35a92gh	198	4	uses	use	NNS
cord-009376-a35a92gh	198	5	activated	activate	VBN
cord-009376-a35a92gh	198	6	calf	calf	NN
cord-009376-a35a92gh	198	7	thymus	thymus	NN
cord-009376-a35a92gh	198	8	DNA	dna	NN
cord-009376-a35a92gh	198	9	to	to	TO
cord-009376-a35a92gh	198	10	suppress	suppress	VB
cord-009376-a35a92gh	198	11	the	the	DT
cord-009376-a35a92gh	198	12	RT	rt	NN
cord-009376-a35a92gh	198	13	-	-	HYPH
cord-009376-a35a92gh	198	14	like	like	JJ
cord-009376-a35a92gh	198	15	activity	activity	NN
cord-009376-a35a92gh	198	16	from	from	IN
cord-009376-a35a92gh	198	17	cellular	cellular	JJ
cord-009376-a35a92gh	198	18	and	and	CC
cord-009376-a35a92gh	198	19	Taq	taq	NN
cord-009376-a35a92gh	198	20	DNA	DNA	NNP
cord-009376-a35a92gh	199	1	polymerases	polymerase	NNS
cord-009376-a35a92gh	199	2	with	with	IN
cord-009376-a35a92gh	199	3	no	no	DT
cord-009376-a35a92gh	199	4	Ž	ž	NN
cord-009376-a35a92gh	199	5	.	.	.
cord-009376-a35a92gh	200	1	reduction	reduction	NN
cord-009376-a35a92gh	200	2	in	in	IN
cord-009376-a35a92gh	200	3	the	the	DT
cord-009376-a35a92gh	200	4	RT	RT	NNP
cord-009376-a35a92gh	200	5	activity	activity	NN
cord-009376-a35a92gh	200	6	Table	Table	NNP
cord-009376-a35a92gh	200	7	4	4	CD
cord-009376-a35a92gh	200	8	.	.	.
cord-009376-a35a92gh	201	1	A	a	DT
cord-009376-a35a92gh	201	2	linear	linear	JJ
cord-009376-a35a92gh	201	3	Ž	ž	NN
cord-009376-a35a92gh	201	4	.	.	.
cord-009376-a35a92gh	202	1	relationship	relationship	NN
cord-009376-a35a92gh	202	2	between	between	IN
cord-009376-a35a92gh	202	3	threshold	threshold	NN
cord-009376-a35a92gh	202	4	cycle	cycle	NN
cord-009376-a35a92gh	202	5	C	C	NNP
cord-009376-a35a92gh	202	6	and	and	CC
cord-009376-a35a92gh	202	7	the	the	DT
cord-009376-a35a92gh	202	8	T	T	NNP
cord-009376-a35a92gh	202	9	number	number	NN
cord-009376-a35a92gh	202	10	of	of	IN
cord-009376-a35a92gh	202	11	MLV	MLV	NNP
cord-009376-a35a92gh	202	12	retrovirus	retrovirus	NN
cord-009376-a35a92gh	202	13	particles	particle	NNS
cord-009376-a35a92gh	202	14	or	or	CC
cord-009376-a35a92gh	202	15	RT	RT	NNP
cord-009376-a35a92gh	202	16	and	and	CC
cord-009376-a35a92gh	202	17	PERV	PERV	NNP
cord-009376-a35a92gh	202	18	is	be	VBZ
cord-009376-a35a92gh	202	19	approximately	approximately	RB
cord-009376-a35a92gh	202	20	1000	1000	CD
cord-009376-a35a92gh	202	21	virion	virion	NN
cord-009376-a35a92gh	202	22	particles	particle	NNS
cord-009376-a35a92gh	202	23	and	and	CC
cord-009376-a35a92gh	202	24	1000	1000	CD
cord-009376-a35a92gh	202	25	᎐	᎐	CD
cord-009376-a35a92gh	202	26	10	10	CD
cord-009376-a35a92gh	202	27	000	000	CD
cord-009376-a35a92gh	202	28	molecules	molecule	NNS
cord-009376-a35a92gh	202	29	of	of	IN
cord-009376-a35a92gh	202	30	purified	purify	VBN
cord-009376-a35a92gh	202	31	AMV	AMV	NNP
cord-009376-a35a92gh	202	32	RT	RT	NNP
cord-009376-a35a92gh	202	33	enzyme	enzyme	NN
cord-009376-a35a92gh	202	34	,	,	,
cord-009376-a35a92gh	202	35	however	however	RB
cord-009376-a35a92gh	202	36	,	,	,
cord-009376-a35a92gh	202	37	the	the	DT
cord-009376-a35a92gh	202	38	assay	assay	NN
cord-009376-a35a92gh	202	39	can	can	MD
cord-009376-a35a92gh	202	40	detect	detect	VB
cord-009376-a35a92gh	202	41	lower	low	JJR
cord-009376-a35a92gh	202	42	levels	level	NNS
cord-009376-a35a92gh	202	43	of	of	IN
cord-009376-a35a92gh	202	44	retrovirus	retrovirus	NN
cord-009376-a35a92gh	202	45	with	with	IN
cord-009376-a35a92gh	202	46	decreased	decreased	JJ
cord-009376-a35a92gh	202	47	frequency	frequency	NN
cord-009376-a35a92gh	202	48	.	.	.
cord-009376-a35a92gh	203	1	An	an	DT
cord-009376-a35a92gh	203	2	overview	overview	NN
cord-009376-a35a92gh	203	3	of	of	IN
cord-009376-a35a92gh	203	4	the	the	DT
cord-009376-a35a92gh	203	5	F	F	NNP
cord-009376-a35a92gh	203	6	-	-	HYPH
cord-009376-a35a92gh	203	7	PERT	pert	JJ
cord-009376-a35a92gh	203	8	assay	assay	NN
cord-009376-a35a92gh	203	9	is	be	VBZ
cord-009376-a35a92gh	203	10	described	describe	VBN
cord-009376-a35a92gh	203	11	in	in	IN
cord-009376-a35a92gh	203	12	a	a	DT
cord-009376-a35a92gh	203	13	flow	flow	NN
cord-009376-a35a92gh	203	14	diagram	diagram	NN
cord-009376-a35a92gh	203	15	in	in	IN
cord-009376-a35a92gh	203	16	Fig	Fig	NNP
cord-009376-a35a92gh	203	17	.	.	.
cord-009376-a35a92gh	204	1	4	4	LS
cord-009376-a35a92gh	204	2	.	.	.
cord-009376-a35a92gh	205	1	The	the	DT
cord-009376-a35a92gh	205	2	high	high	JJ
cord-009376-a35a92gh	205	3	sensitivity	sensitivity	NN
cord-009376-a35a92gh	205	4	of	of	IN
cord-009376-a35a92gh	205	5	F	F	NNP
cord-009376-a35a92gh	205	6	-	-	HYPH
cord-009376-a35a92gh	205	7	PERT	PERT	NNP
cord-009376-a35a92gh	205	8	has	have	VBZ
cord-009376-a35a92gh	205	9	ensured	ensure	VBN
cord-009376-a35a92gh	205	10	that	that	IN
cord-009376-a35a92gh	205	11	this	this	DT
cord-009376-a35a92gh	205	12	Q	Q	NNP
cord-009376-a35a92gh	205	13	-	-	HYPH
cord-009376-a35a92gh	205	14	PCR	PCR	NNP
cord-009376-a35a92gh	205	15	assay	assay	NN
cord-009376-a35a92gh	205	16	plays	play	VBZ
cord-009376-a35a92gh	205	17	a	a	DT
cord-009376-a35a92gh	205	18	significant	significant	JJ
cord-009376-a35a92gh	205	19	role	role	NN
cord-009376-a35a92gh	205	20	in	in	IN
cord-009376-a35a92gh	205	21	the	the	DT
cord-009376-a35a92gh	205	22	evaluation	evaluation	NN
cord-009376-a35a92gh	205	23	of	of	IN
cord-009376-a35a92gh	205	24	the	the	DT
cord-009376-a35a92gh	205	25	retroviral	retroviral	JJ
cord-009376-a35a92gh	205	26	status	status	NN
cord-009376-a35a92gh	205	27	of	of	IN
cord-009376-a35a92gh	205	28	vaccines	vaccine	NNS
cord-009376-a35a92gh	205	29	and	and	CC
cord-009376-a35a92gh	205	30	other	other	JJ
cord-009376-a35a92gh	205	31	biological	biological	JJ
cord-009376-a35a92gh	205	32	material	material	NN
cord-009376-a35a92gh	205	33	from	from	IN
cord-009376-a35a92gh	205	34	the	the	DT
cord-009376-a35a92gh	205	35	biotechnology	biotechnology	NN
cord-009376-a35a92gh	205	36	industry	industry	NN
cord-009376-a35a92gh	205	37	,	,	,
cord-009376-a35a92gh	205	38	where	where	WRB
cord-009376-a35a92gh	205	39	safety	safety	NN
cord-009376-a35a92gh	205	40	of	of	IN
cord-009376-a35a92gh	205	41	products	product	NNS
cord-009376-a35a92gh	205	42	is	be	VBZ
cord-009376-a35a92gh	205	43	paramount	paramount	JJ
cord-009376-a35a92gh	205	44	.	.	.
cord-009376-a35a92gh	206	1	More	more	RBR
cord-009376-a35a92gh	206	2	specifically	specifically	RB
cord-009376-a35a92gh	206	3	,	,	,
cord-009376-a35a92gh	206	4	when	when	WRB
cord-009376-a35a92gh	206	5	performed	perform	VBN
cord-009376-a35a92gh	206	6	in	in	IN
cord-009376-a35a92gh	206	7	combination	combination	NN
cord-009376-a35a92gh	206	8	with	with	IN
cord-009376-a35a92gh	206	9	co	co	NN
cord-009376-a35a92gh	206	10	-	-	NN
cord-009376-a35a92gh	206	11	cultivation	cultivation	NN
cord-009376-a35a92gh	206	12	,	,	,
cord-009376-a35a92gh	206	13	induction	induction	NN
cord-009376-a35a92gh	206	14	and	and	CC
cord-009376-a35a92gh	206	15	infectivity	infectivity	NN
cord-009376-a35a92gh	206	16	assays	assay	NNS
cord-009376-a35a92gh	206	17	,	,	,
cord-009376-a35a92gh	206	18	together	together	RB
cord-009376-a35a92gh	206	19	with	with	IN
cord-009376-a35a92gh	206	20	electron	electron	NN
cord-009376-a35a92gh	206	21	microscopy	microscopy	NN
cord-009376-a35a92gh	206	22	Ž	ž	NN
cord-009376-a35a92gh	206	23	.	.	.
cord-009376-a35a92gh	207	1	EM	EM	NNP
cord-009376-a35a92gh	207	2	and	and	CC
cord-009376-a35a92gh	207	3	pathogen	pathogen	NN
cord-009376-a35a92gh	207	4	-	-	HYPH
cord-009376-a35a92gh	207	5	specific	specific	JJ
cord-009376-a35a92gh	207	6	PCR	PCR	NNP
cord-009376-a35a92gh	207	7	detection	detection	NN
cord-009376-a35a92gh	207	8	assays	assay	NNS
cord-009376-a35a92gh	207	9	,	,	,
cord-009376-a35a92gh	207	10	F	F	NNP
cord-009376-a35a92gh	207	11	-	-	HYPH
cord-009376-a35a92gh	207	12	PERT	pert	JJ
cord-009376-a35a92gh	207	13	assists	assist	VBZ
cord-009376-a35a92gh	207	14	in	in	IN
cord-009376-a35a92gh	207	15	providing	provide	VBG
cord-009376-a35a92gh	207	16	a	a	DT
cord-009376-a35a92gh	207	17	detailed	detailed	JJ
cord-009376-a35a92gh	207	18	risk	risk	NN
cord-009376-a35a92gh	207	19	assessment	assessment	NN
cord-009376-a35a92gh	207	20	for	for	IN
cord-009376-a35a92gh	207	21	the	the	DT
cord-009376-a35a92gh	207	22	presence	presence	NN
cord-009376-a35a92gh	207	23	of	of	IN
cord-009376-a35a92gh	207	24	adventitious	adventitious	JJ
cord-009376-a35a92gh	207	25	agents	agent	NNS
cord-009376-a35a92gh	207	26	in	in	IN
cord-009376-a35a92gh	207	27	therapeutics	therapeutic	NNS
cord-009376-a35a92gh	207	28	.	.	.
cord-009376-a35a92gh	208	1	Viruses	virus	NNS
cord-009376-a35a92gh	208	2	may	may	MD
cord-009376-a35a92gh	208	3	contaminate	contaminate	VB
cord-009376-a35a92gh	208	4	cell	cell	NN
cord-009376-a35a92gh	208	5	lines	line	NNS
cord-009376-a35a92gh	208	6	from	from	IN
cord-009376-a35a92gh	208	7	a	a	DT
cord-009376-a35a92gh	208	8	Ž	ž	NN
cord-009376-a35a92gh	208	9	.	.	.
cord-009376-a35a92gh	209	1	number	number	NN
cord-009376-a35a92gh	209	2	of	of	IN
cord-009376-a35a92gh	209	3	sources	source	NNS
cord-009376-a35a92gh	209	4	including	include	VBG
cord-009376-a35a92gh	209	5	:	:	:
cord-009376-a35a92gh	209	6	1	1	CD
cord-009376-a35a92gh	209	7	those	those	DT
cord-009376-a35a92gh	209	8	present	present	JJ
cord-009376-a35a92gh	209	9	in	in	IN
cord-009376-a35a92gh	209	10	2	2	CD
cord-009376-a35a92gh	209	11	viruses	virus	NNS
cord-009376-a35a92gh	209	12	of	of	IN
cord-009376-a35a92gh	209	13	animal	animal	NN
cord-009376-a35a92gh	209	14	origin	origin	NN
cord-009376-a35a92gh	209	15	in	in	IN
cord-009376-a35a92gh	209	16	media	medium	NNS
cord-009376-a35a92gh	209	17	and	and	CC
cord-009376-a35a92gh	209	18	tissue	tissue	NN
cord-009376-a35a92gh	209	19	Ž	ž	NN
cord-009376-a35a92gh	209	20	.	.	.
cord-009376-a35a92gh	210	1	culture	culture	NN
cord-009376-a35a92gh	210	2	reagents	reagent	NNS
cord-009376-a35a92gh	210	3	;	;	:
cord-009376-a35a92gh	210	4	and	and	CC
cord-009376-a35a92gh	210	5	3	3	CD
cord-009376-a35a92gh	210	6	those	those	DT
cord-009376-a35a92gh	210	7	from	from	IN
cord-009376-a35a92gh	210	8	operators	operator	NNS
cord-009376-a35a92gh	210	9	or	or	CC
cord-009376-a35a92gh	210	10	other	other	JJ
cord-009376-a35a92gh	210	11	sources	source	NNS
cord-009376-a35a92gh	210	12	that	that	WDT
cord-009376-a35a92gh	210	13	could	could	MD
cord-009376-a35a92gh	210	14	have	have	VB
cord-009376-a35a92gh	210	15	contaminated	contaminate	VBN
cord-009376-a35a92gh	210	16	the	the	DT
cord-009376-a35a92gh	210	17	cells	cell	NNS
cord-009376-a35a92gh	210	18	during	during	IN
cord-009376-a35a92gh	210	19	handling	handle	VBG
cord-009376-a35a92gh	210	20	in	in	IN
cord-009376-a35a92gh	210	21	non	non	JJ
cord-009376-a35a92gh	210	22	-	-	JJ
cord-009376-a35a92gh	210	23	GMP	gmp	JJ
cord-009376-a35a92gh	210	24	conditions	condition	NNS
cord-009376-a35a92gh	210	25	.	.	.
cord-009376-a35a92gh	211	1	For	for	IN
cord-009376-a35a92gh	211	2	these	these	DT
cord-009376-a35a92gh	211	3	reasons	reason	NNS
cord-009376-a35a92gh	211	4	,	,	,
cord-009376-a35a92gh	211	5	all	all	DT
cord-009376-a35a92gh	211	6	cell	cell	NN
cord-009376-a35a92gh	211	7	lines	line	NNS
cord-009376-a35a92gh	211	8	,	,	,
cord-009376-a35a92gh	211	9	primary	primary	JJ
cord-009376-a35a92gh	211	10	cells	cell	NNS
cord-009376-a35a92gh	211	11	,	,	,
cord-009376-a35a92gh	211	12	or	or	CC
cord-009376-a35a92gh	211	13	tissue	tissue	NN
cord-009376-a35a92gh	211	14	products	product	NNS
cord-009376-a35a92gh	211	15	used	use	VBN
cord-009376-a35a92gh	211	16	for	for	IN
cord-009376-a35a92gh	211	17	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	211	18	purposes	purpose	NNS
cord-009376-a35a92gh	211	19	require	require	VBP
cord-009376-a35a92gh	211	20	testing	testing	NN
cord-009376-a35a92gh	211	21	for	for	IN
cord-009376-a35a92gh	211	22	the	the	DT
cord-009376-a35a92gh	211	23	presence	presence	NN
cord-009376-a35a92gh	211	24	of	of	IN
cord-009376-a35a92gh	211	25	a	a	DT
cord-009376-a35a92gh	211	26	range	range	NN
cord-009376-a35a92gh	211	27	of	of	IN
cord-009376-a35a92gh	211	28	viruses	virus	NNS
cord-009376-a35a92gh	211	29	.	.	.
cord-009376-a35a92gh	212	1	The	the	DT
cord-009376-a35a92gh	212	2	selection	selection	NN
cord-009376-a35a92gh	212	3	of	of	IN
cord-009376-a35a92gh	212	4	viruses	virus	NNS
cord-009376-a35a92gh	212	5	to	to	TO
cord-009376-a35a92gh	212	6	be	be	VB
cord-009376-a35a92gh	212	7	tested	test	VBN
cord-009376-a35a92gh	212	8	depends	depend	VBZ
cord-009376-a35a92gh	212	9	upon	upon	IN
cord-009376-a35a92gh	212	10	the	the	DT
cord-009376-a35a92gh	212	11	origin	origin	NN
cord-009376-a35a92gh	212	12	of	of	IN
cord-009376-a35a92gh	212	13	the	the	DT
cord-009376-a35a92gh	212	14	cell	cell	NN
cord-009376-a35a92gh	212	15	line	line	NN
cord-009376-a35a92gh	212	16	and	and	CC
cord-009376-a35a92gh	212	17	raw	raw	JJ
cord-009376-a35a92gh	212	18	material	material	NN
cord-009376-a35a92gh	212	19	used	use	VBN
cord-009376-a35a92gh	212	20	in	in	IN
cord-009376-a35a92gh	212	21	manufacture	manufacture	NN
cord-009376-a35a92gh	212	22	.	.	.
cord-009376-a35a92gh	213	1	The	the	DT
cord-009376-a35a92gh	213	2	development	development	NN
cord-009376-a35a92gh	213	3	and	and	CC
cord-009376-a35a92gh	213	4	validation	validation	NN
cord-009376-a35a92gh	213	5	of	of	IN
cord-009376-a35a92gh	213	6	PCR	PCR	NNP
cord-009376-a35a92gh	213	7	assays	assay	NNS
cord-009376-a35a92gh	213	8	to	to	TO
cord-009376-a35a92gh	213	9	meet	meet	VB
cord-009376-a35a92gh	213	10	the	the	DT
cord-009376-a35a92gh	213	11	requirements	requirement	NNS
cord-009376-a35a92gh	213	12	of	of	IN
cord-009376-a35a92gh	213	13	regulatory	regulatory	JJ
cord-009376-a35a92gh	213	14	authorities	authority	NNS
cord-009376-a35a92gh	213	15	is	be	VBZ
cord-009376-a35a92gh	213	16	a	a	DT
cord-009376-a35a92gh	213	17	key	key	JJ
cord-009376-a35a92gh	213	18	element	element	NN
cord-009376-a35a92gh	213	19	in	in	IN
cord-009376-a35a92gh	213	20	the	the	DT
cord-009376-a35a92gh	213	21	production	production	NN
cord-009376-a35a92gh	213	22	and	and	CC
cord-009376-a35a92gh	213	23	marketing	marketing	NN
cord-009376-a35a92gh	213	24	of	of	IN
cord-009376-a35a92gh	213	25	final	final	JJ
cord-009376-a35a92gh	213	26	products	product	NNS
cord-009376-a35a92gh	213	27	.	.	.
cord-009376-a35a92gh	214	1	An	an	DT
cord-009376-a35a92gh	214	2	increasing	increase	VBG
cord-009376-a35a92gh	214	3	number	number	NN
cord-009376-a35a92gh	214	4	of	of	IN
cord-009376-a35a92gh	214	5	published	publish	VBN
cord-009376-a35a92gh	214	6	or	or	CC
cord-009376-a35a92gh	214	7	validated	validate	VBN
cord-009376-a35a92gh	214	8	Q	Q	NNP
cord-009376-a35a92gh	214	9	-	-	HYPH
cord-009376-a35a92gh	214	10	PCR	PCR	NNP
cord-009376-a35a92gh	214	11	assays	assay	NNS
cord-009376-a35a92gh	214	12	are	be	VBP
cord-009376-a35a92gh	214	13	becoming	become	VBG
cord-009376-a35a92gh	214	14	available	available	JJ
cord-009376-a35a92gh	214	15	,	,	,
cord-009376-a35a92gh	214	16	and	and	CC
cord-009376-a35a92gh	214	17	these	these	DT
cord-009376-a35a92gh	214	18	tests	test	NNS
cord-009376-a35a92gh	214	19	can	can	MD
cord-009376-a35a92gh	214	20	be	be	VB
cord-009376-a35a92gh	214	21	utilised	utilise	VBN
cord-009376-a35a92gh	214	22	for	for	IN
cord-009376-a35a92gh	214	23	cell	cell	NN
cord-009376-a35a92gh	214	24	line	line	NN
cord-009376-a35a92gh	214	25	,	,	,
cord-009376-a35a92gh	214	26	raw	raw	JJ
cord-009376-a35a92gh	214	27	material	material	NN
cord-009376-a35a92gh	214	28	,	,	,
cord-009376-a35a92gh	214	29	or	or	CC
cord-009376-a35a92gh	214	30	Ž	ž	NN
cord-009376-a35a92gh	214	31	.	.	.
cord-009376-a35a92gh	215	1	final	final	JJ
cord-009376-a35a92gh	215	2	product	product	NN
cord-009376-a35a92gh	215	3	testing	test	VBG
cord-009376-a35a92gh	215	4	Tables	Tables	NNPS
cord-009376-a35a92gh	215	5	5	5	CD
cord-009376-a35a92gh	215	6	and	and	CC
cord-009376-a35a92gh	215	7	6	6	CD
cord-009376-a35a92gh	215	8	.	.	.
cord-009376-a35a92gh	216	1	Testing	test	VBG
cord-009376-a35a92gh	216	2	for	for	IN
cord-009376-a35a92gh	216	3	the	the	DT
cord-009376-a35a92gh	216	4	presence	presence	NN
cord-009376-a35a92gh	216	5	of	of	IN
cord-009376-a35a92gh	216	6	human	human	JJ
cord-009376-a35a92gh	216	7	viruses	virus	NNS
cord-009376-a35a92gh	216	8	is	be	VBZ
cord-009376-a35a92gh	216	9	required	require	VBN
cord-009376-a35a92gh	216	10	when	when	WRB
cord-009376-a35a92gh	216	11	cells	cell	NNS
cord-009376-a35a92gh	216	12	of	of	IN
cord-009376-a35a92gh	216	13	human	human	JJ
cord-009376-a35a92gh	216	14	origin	origin	NN
cord-009376-a35a92gh	216	15	or	or	CC
cord-009376-a35a92gh	216	16	products	product	NNS
cord-009376-a35a92gh	216	17	obtained	obtain	VBN
cord-009376-a35a92gh	216	18	from	from	IN
cord-009376-a35a92gh	216	19	human	human	JJ
cord-009376-a35a92gh	216	20	blood	blood	NN
cord-009376-a35a92gh	216	21	or	or	CC
cord-009376-a35a92gh	216	22	tissues	tissue	NNS
cord-009376-a35a92gh	216	23	are	be	VBP
cord-009376-a35a92gh	216	24	involved	involve	VBN
cord-009376-a35a92gh	216	25	.	.	.
cord-009376-a35a92gh	217	1	Examples	example	NNS
cord-009376-a35a92gh	217	2	include	include	VBP
cord-009376-a35a92gh	217	3	mouse	mouse	NN
cord-009376-a35a92gh	217	4	᎐	᎐	CD
cord-009376-a35a92gh	217	5	human	human	JJ
cord-009376-a35a92gh	217	6	and	and	CC
cord-009376-a35a92gh	217	7	human	human	JJ
cord-009376-a35a92gh	217	8	᎐	᎐	CD
cord-009376-a35a92gh	217	9	human	human	NNP
cord-009376-a35a92gh	217	10	hybridomas	hybridomas	NNP
cord-009376-a35a92gh	217	11	,	,	,
cord-009376-a35a92gh	217	12	and	and	CC
cord-009376-a35a92gh	217	13	cell	cell	NN
cord-009376-a35a92gh	217	14	lines	line	NNS
cord-009376-a35a92gh	217	15	of	of	IN
cord-009376-a35a92gh	217	16	human	human	JJ
cord-009376-a35a92gh	217	17	origin	origin	NN
cord-009376-a35a92gh	217	18	such	such	JJ
cord-009376-a35a92gh	217	19	as	as	IN
cord-009376-a35a92gh	217	20	MRC-5	MRC-5	NNP
cord-009376-a35a92gh	217	21	,	,	,
cord-009376-a35a92gh	217	22	and	and	CC
cord-009376-a35a92gh	218	1	PER.C6	per.c6	IN
cord-009376-a35a92gh	218	2	used	use	VBN
cord-009376-a35a92gh	218	3	in	in	IN
cord-009376-a35a92gh	218	4	the	the	DT
cord-009376-a35a92gh	218	5	production	production	NN
cord-009376-a35a92gh	218	6	of	of	IN
cord-009376-a35a92gh	218	7	Adenovirus	Adenovirus	NNP
cord-009376-a35a92gh	218	8	vectors	vector	NNS
cord-009376-a35a92gh	218	9	for	for	IN
cord-009376-a35a92gh	218	10	therapy	therapy	NN
cord-009376-a35a92gh	218	11	.	.	.
cord-009376-a35a92gh	219	1	In	in	IN
cord-009376-a35a92gh	219	2	broad	broad	JJ
cord-009376-a35a92gh	219	3	terms	term	NNS
cord-009376-a35a92gh	219	4	,	,	,
cord-009376-a35a92gh	219	5	the	the	DT
cord-009376-a35a92gh	219	6	viruses	virus	NNS
cord-009376-a35a92gh	219	7	that	that	WDT
cord-009376-a35a92gh	219	8	have	have	VBP
cord-009376-a35a92gh	219	9	to	to	TO
cord-009376-a35a92gh	219	10	be	be	VB
cord-009376-a35a92gh	219	11	screened	screen	VBN
cord-009376-a35a92gh	219	12	for	for	IN
cord-009376-a35a92gh	219	13	in	in	IN
cord-009376-a35a92gh	219	14	a	a	DT
cord-009376-a35a92gh	219	15	human	human	JJ
cord-009376-a35a92gh	219	16	cell	cell	NN
cord-009376-a35a92gh	219	17	line	line	NN
cord-009376-a35a92gh	219	18	are	be	VBP
cord-009376-a35a92gh	219	19	those	those	DT
cord-009376-a35a92gh	219	20	associated	associate	VBN
cord-009376-a35a92gh	219	21	with	with	IN
cord-009376-a35a92gh	219	22	severe	severe	JJ
cord-009376-a35a92gh	219	23	or	or	CC
cord-009376-a35a92gh	219	24	oncogenic	oncogenic	JJ
cord-009376-a35a92gh	219	25	diseases	disease	NNS
cord-009376-a35a92gh	219	26	,	,	,
cord-009376-a35a92gh	219	27	and	and	CC
cord-009376-a35a92gh	219	28	those	those	DT
cord-009376-a35a92gh	219	29	that	that	WDT
cord-009376-a35a92gh	219	30	are	be	VBP
cord-009376-a35a92gh	219	31	likely	likely	JJ
cord-009376-a35a92gh	219	32	to	to	TO
cord-009376-a35a92gh	219	33	infect	infect	VB
cord-009376-a35a92gh	219	34	the	the	DT
cord-009376-a35a92gh	219	35	cell	cell	NN
cord-009376-a35a92gh	219	36	type	type	NN
cord-009376-a35a92gh	219	37	,	,	,
cord-009376-a35a92gh	219	38	particularly	particularly	RB
cord-009376-a35a92gh	219	39	those	those	DT
cord-009376-a35a92gh	219	40	that	that	WDT
cord-009376-a35a92gh	219	41	might	may	MD
cord-009376-a35a92gh	219	42	establish	establish	VB
cord-009376-a35a92gh	219	43	latent	latent	JJ
cord-009376-a35a92gh	219	44	or	or	CC
cord-009376-a35a92gh	219	45	abortive	abortive	JJ
cord-009376-a35a92gh	219	46	replication	replication	NN
cord-009376-a35a92gh	219	47	in	in	IN
cord-009376-a35a92gh	219	48	cells	cell	NNS
cord-009376-a35a92gh	219	49	.	.	.
cord-009376-a35a92gh	220	1	Animal	animal	NN
cord-009376-a35a92gh	220	2	viruses	virus	NNS
cord-009376-a35a92gh	220	3	pose	pose	VBP
cord-009376-a35a92gh	220	4	significant	significant	JJ
cord-009376-a35a92gh	220	5	risk	risk	NN
cord-009376-a35a92gh	220	6	if	if	IN
cord-009376-a35a92gh	220	7	the	the	DT
cord-009376-a35a92gh	220	8	cell	cell	NN
cord-009376-a35a92gh	220	9	line	line	NN
cord-009376-a35a92gh	220	10	or	or	CC
cord-009376-a35a92gh	220	11	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	220	12	is	be	VBZ
cord-009376-a35a92gh	220	13	of	of	IN
cord-009376-a35a92gh	220	14	animal	animal	NN
cord-009376-a35a92gh	220	15	origin	origin	NN
cord-009376-a35a92gh	220	16	andror	andror	NNP
cord-009376-a35a92gh	220	17	if	if	IN
cord-009376-a35a92gh	220	18	animal	animal	NN
cord-009376-a35a92gh	220	19	products	product	NNS
cord-009376-a35a92gh	220	20	such	such	JJ
cord-009376-a35a92gh	220	21	as	as	IN
cord-009376-a35a92gh	220	22	foetal	foetal	JJ
cord-009376-a35a92gh	220	23	calf	calf	NN
cord-009376-a35a92gh	220	24	serum	serum	NN
cord-009376-a35a92gh	220	25	,	,	,
cord-009376-a35a92gh	220	26	trypsin	trypsin	NN
cord-009376-a35a92gh	220	27	,	,	,
cord-009376-a35a92gh	220	28	or	or	CC
cord-009376-a35a92gh	220	29	insulin	insulin	NN
cord-009376-a35a92gh	220	30	are	be	VBP
cord-009376-a35a92gh	220	31	used	use	VBN
cord-009376-a35a92gh	220	32	in	in	IN
cord-009376-a35a92gh	220	33	the	the	DT
cord-009376-a35a92gh	220	34	manufacturing	manufacturing	NN
cord-009376-a35a92gh	220	35	cultures	culture	NNS
cord-009376-a35a92gh	220	36	.	.	.
cord-009376-a35a92gh	221	1	All	all	DT
cord-009376-a35a92gh	221	2	new	new	JJ
cord-009376-a35a92gh	221	3	animal	animal	NN
cord-009376-a35a92gh	221	4	reagents	reagent	NNS
cord-009376-a35a92gh	221	5	used	use	VBN
cord-009376-a35a92gh	221	6	in	in	IN
cord-009376-a35a92gh	221	7	manufacture	manufacture	NN
cord-009376-a35a92gh	221	8	should	should	MD
cord-009376-a35a92gh	221	9	be	be	VB
cord-009376-a35a92gh	221	10	screened	screen	VBN
cord-009376-a35a92gh	221	11	by	by	IN
cord-009376-a35a92gh	221	12	appropriate	appropriate	JJ
cord-009376-a35a92gh	221	13	techniques	technique	NNS
cord-009376-a35a92gh	221	14	.	.	.
cord-009376-a35a92gh	222	1	Bovine	bovine	JJ
cord-009376-a35a92gh	222	2	serum	serum	NN
cord-009376-a35a92gh	222	3	and	and	CC
cord-009376-a35a92gh	222	4	porcine	porcine	JJ
cord-009376-a35a92gh	222	5	trypsin	trypsin	NN
cord-009376-a35a92gh	222	6	are	be	VBP
cord-009376-a35a92gh	222	7	usually	usually	RB
cord-009376-a35a92gh	222	8	screened	screen	VBN
cord-009376-a35a92gh	222	9	using	use	VBG
cord-009376-a35a92gh	222	10	an	an	DT
cord-009376-a35a92gh	222	11	appropriate	appropriate	JJ
cord-009376-a35a92gh	222	12	Title	title	NN
cord-009376-a35a92gh	222	13	9	9	CD
cord-009376-a35a92gh	222	14	Code	Code	NNP
cord-009376-a35a92gh	222	15	of	of	IN
cord-009376-a35a92gh	222	16	Ž	Ž	NNP
cord-009376-a35a92gh	222	17	.	.	.
cord-009376-a35a92gh	223	1	Federal	Federal	NNP
cord-009376-a35a92gh	223	2	Regulation	Regulation	NNP
cord-009376-a35a92gh	223	3	9CFR	9cfr	CD
cord-009376-a35a92gh	223	4	assay	assay	NN
cord-009376-a35a92gh	223	5	.	.	.
cord-009376-a35a92gh	224	1	However	however	RB
cord-009376-a35a92gh	224	2	,	,	,
cord-009376-a35a92gh	224	3	some	some	DT
cord-009376-a35a92gh	224	4	caution	caution	NN
cord-009376-a35a92gh	224	5	is	be	VBZ
cord-009376-a35a92gh	224	6	required	require	VBN
cord-009376-a35a92gh	224	7	as	as	IN
cord-009376-a35a92gh	224	8	9CFR	9cfr	XX
cord-009376-a35a92gh	224	9	assays	assay	NNS
cord-009376-a35a92gh	224	10	using	use	VBG
cord-009376-a35a92gh	224	11	minimal	minimal	JJ
cord-009376-a35a92gh	224	12	volumes	volume	NNS
cord-009376-a35a92gh	224	13	may	may	MD
cord-009376-a35a92gh	224	14	miss	miss	VB
cord-009376-a35a92gh	224	15	critical	critical	JJ
cord-009376-a35a92gh	224	16	viruses	virus	NNS
cord-009376-a35a92gh	224	17	.	.	.
cord-009376-a35a92gh	225	1	In	in	IN
cord-009376-a35a92gh	225	2	addition	addition	NN
cord-009376-a35a92gh	225	3	,	,	,
cord-009376-a35a92gh	225	4	some	some	DT
cord-009376-a35a92gh	225	5	viruses	virus	NNS
cord-009376-a35a92gh	225	6	are	be	VBP
cord-009376-a35a92gh	225	7	not	not	RB
cord-009376-a35a92gh	225	8	detected	detect	VBN
cord-009376-a35a92gh	225	9	by	by	IN
cord-009376-a35a92gh	225	10	these	these	DT
cord-009376-a35a92gh	225	11	assays	assay	NNS
cord-009376-a35a92gh	225	12	,	,	,
cord-009376-a35a92gh	225	13	therefore	therefore	RB
cord-009376-a35a92gh	225	14	PCR	PCR	NNP
cord-009376-a35a92gh	225	15	should	should	MD
cord-009376-a35a92gh	225	16	be	be	VB
cord-009376-a35a92gh	225	17	employed	employ	VBN
cord-009376-a35a92gh	225	18	.	.	.
cord-009376-a35a92gh	226	1	Many	many	JJ
cord-009376-a35a92gh	226	2	viruses	virus	NNS
cord-009376-a35a92gh	226	3	are	be	VBP
cord-009376-a35a92gh	226	4	of	of	IN
cord-009376-a35a92gh	226	5	concern	concern	NN
cord-009376-a35a92gh	226	6	in	in	IN
cord-009376-a35a92gh	226	7	the	the	DT
cord-009376-a35a92gh	226	8	manufacturing	manufacturing	NN
cord-009376-a35a92gh	226	9	process	process	NN
cord-009376-a35a92gh	226	10	and	and	CC
cord-009376-a35a92gh	226	11	are	be	VBP
cord-009376-a35a92gh	226	12	not	not	RB
cord-009376-a35a92gh	226	13	the	the	DT
cord-009376-a35a92gh	226	14	subject	subject	NN
cord-009376-a35a92gh	226	15	of	of	IN
cord-009376-a35a92gh	226	16	this	this	DT
cord-009376-a35a92gh	226	17	review	review	NN
cord-009376-a35a92gh	226	18	.	.	.
cord-009376-a35a92gh	227	1	Instead	instead	RB
cord-009376-a35a92gh	227	2	,	,	,
cord-009376-a35a92gh	227	3	this	this	DT
cord-009376-a35a92gh	227	4	section	section	NN
cord-009376-a35a92gh	227	5	focuses	focus	VBZ
cord-009376-a35a92gh	227	6	on	on	IN
cord-009376-a35a92gh	227	7	examples	example	NNS
cord-009376-a35a92gh	227	8	of	of	IN
cord-009376-a35a92gh	227	9	viruses	virus	NNS
cord-009376-a35a92gh	227	10	that	that	WDT
cord-009376-a35a92gh	227	11	have	have	VBP
cord-009376-a35a92gh	227	12	recently	recently	RB
cord-009376-a35a92gh	227	13	emerged	emerge	VBN
cord-009376-a35a92gh	227	14	as	as	IN
cord-009376-a35a92gh	227	15	agents	agent	NNS
cord-009376-a35a92gh	227	16	of	of	IN
cord-009376-a35a92gh	227	17	concern	concern	NN
cord-009376-a35a92gh	227	18	,	,	,
cord-009376-a35a92gh	227	19	namely	namely	RB
cord-009376-a35a92gh	227	20	Bovine	bovine	JJ
cord-009376-a35a92gh	227	21	polyomavirus	polyomavirus	NN
cord-009376-a35a92gh	227	22	,	,	,
cord-009376-a35a92gh	227	23	Bunyavirus	Bunyavirus	NNP
cord-009376-a35a92gh	227	24	and	and	CC
cord-009376-a35a92gh	227	25	Porcine	Porcine	NNP
cord-009376-a35a92gh	227	26	circovirus	circovirus	NNP
cord-009376-a35a92gh	227	27	.	.	.
cord-009376-a35a92gh	228	1	Recent	recent	JJ
cord-009376-a35a92gh	228	2	concern	concern	NN
cord-009376-a35a92gh	228	3	has	have	VBZ
cord-009376-a35a92gh	228	4	been	be	VBN
cord-009376-a35a92gh	228	5	expressed	express	VBN
cord-009376-a35a92gh	228	6	about	about	IN
cord-009376-a35a92gh	228	7	the	the	DT
cord-009376-a35a92gh	228	8	contamination	contamination	NN
cord-009376-a35a92gh	228	9	of	of	IN
cord-009376-a35a92gh	228	10	bovine	bovine	JJ
cord-009376-a35a92gh	228	11	products	product	NNS
cord-009376-a35a92gh	228	12	with	with	IN
cord-009376-a35a92gh	228	13	the	the	DT
cord-009376-a35a92gh	228	14	Bovine	Bovine	NNP
cord-009376-a35a92gh	228	15	Ž	ž	NN
cord-009376-a35a92gh	228	16	.	.	.
cord-009376-a35a92gh	229	1	polyomavirus	polyomavirus	NNP
cord-009376-a35a92gh	229	2	BPyV	BPyV	NNP
cord-009376-a35a92gh	229	3	.	.	.
cord-009376-a35a92gh	230	1	This	this	DT
cord-009376-a35a92gh	230	2	virus	virus	NN
cord-009376-a35a92gh	230	3	belongs	belong	VBZ
cord-009376-a35a92gh	230	4	to	to	IN
cord-009376-a35a92gh	230	5	a	a	DT
cord-009376-a35a92gh	230	6	family	family	NN
cord-009376-a35a92gh	230	7	,	,	,
cord-009376-a35a92gh	230	8	some	some	DT
cord-009376-a35a92gh	230	9	of	of	IN
cord-009376-a35a92gh	230	10	which	which	WDT
cord-009376-a35a92gh	230	11	are	be	VBP
cord-009376-a35a92gh	230	12	oncogenic	oncogenic	JJ
cord-009376-a35a92gh	230	13	in	in	IN
cord-009376-a35a92gh	230	14	their	-PRON-	PRP$
cord-009376-a35a92gh	230	15	own	own	JJ
cord-009376-a35a92gh	230	16	host	host	NN
cord-009376-a35a92gh	230	17	or	or	CC
cord-009376-a35a92gh	230	18	in	in	IN
cord-009376-a35a92gh	230	19	heterologous	heterologous	JJ
cord-009376-a35a92gh	230	20	hosts	host	NNS
cord-009376-a35a92gh	230	21	.	.	.
cord-009376-a35a92gh	231	1	Infection	infection	NN
cord-009376-a35a92gh	231	2	of	of	IN
cord-009376-a35a92gh	231	3	cattle	cattle	NNS
cord-009376-a35a92gh	231	4	is	be	VBZ
cord-009376-a35a92gh	231	5	widespread	widespread	JJ
cord-009376-a35a92gh	231	6	,	,	,
cord-009376-a35a92gh	231	7	and	and	CC
cord-009376-a35a92gh	231	8	up	up	IN
cord-009376-a35a92gh	231	9	to	to	TO
cord-009376-a35a92gh	231	10	70	70	CD
cord-009376-a35a92gh	231	11	%	%	NN
cord-009376-a35a92gh	231	12	of	of	IN
cord-009376-a35a92gh	231	13	foetal	foetal	JJ
cord-009376-a35a92gh	231	14	calf	calf	NN
cord-009376-a35a92gh	231	15	serum	serum	NN
cord-009376-a35a92gh	231	16	batches	batch	NNS
cord-009376-a35a92gh	231	17	contain	contain	VBP
cord-009376-a35a92gh	231	18	BPyV	bpyv	JJ
cord-009376-a35a92gh	231	19	sequences	sequence	NNS
cord-009376-a35a92gh	231	20	by	by	IN
cord-009376-a35a92gh	231	21	PCR	PCR	NNP
cord-009376-a35a92gh	231	22	and	and	CC
cord-009376-a35a92gh	231	23	infectious	infectious	JJ
cord-009376-a35a92gh	231	24	virus	virus	NN
cord-009376-a35a92gh	231	25	can	can	MD
cord-009376-a35a92gh	231	26	be	be	VB
cord-009376-a35a92gh	231	27	isolated	isolate	VBN
cord-009376-a35a92gh	231	28	from	from	IN
cord-009376-a35a92gh	231	29	some	some	DT
cord-009376-a35a92gh	231	30	batches	batch	NNS
cord-009376-a35a92gh	231	31	Ž	ž	NN
cord-009376-a35a92gh	231	32	.	.	.
cord-009376-a35a92gh	232	1	Schuurman	Schuurman	NNP
cord-009376-a35a92gh	232	2	et	et	NNP
cord-009376-a35a92gh	232	3	al	al	NNP
cord-009376-a35a92gh	232	4	.	.	NNP
cord-009376-a35a92gh	232	5	,	,	,
cord-009376-a35a92gh	232	6	1991	1991	CD
cord-009376-a35a92gh	232	7	.	.	.
cord-009376-a35a92gh	233	1	The	the	DT
cord-009376-a35a92gh	233	2	most	most	RBS
cord-009376-a35a92gh	233	3	worrying	worrying	JJ
cord-009376-a35a92gh	233	4	feature	feature	NN
cord-009376-a35a92gh	233	5	of	of	IN
cord-009376-a35a92gh	233	6	this	this	DT
cord-009376-a35a92gh	233	7	virus	virus	NN
cord-009376-a35a92gh	233	8	is	be	VBZ
cord-009376-a35a92gh	233	9	that	that	IN
cord-009376-a35a92gh	233	10	it	-PRON-	PRP
cord-009376-a35a92gh	233	11	is	be	VBZ
cord-009376-a35a92gh	233	12	probably	probably	RB
cord-009376-a35a92gh	233	13	zoonotic	zoonotic	JJ
cord-009376-a35a92gh	233	14	.	.	.
cord-009376-a35a92gh	234	1	It	-PRON-	PRP
cord-009376-a35a92gh	234	2	replicates	replicate	VBZ
cord-009376-a35a92gh	234	3	in	in	IN
cord-009376-a35a92gh	234	4	primate	primate	JJ
cord-009376-a35a92gh	234	5	cells	cell	NNS
cord-009376-a35a92gh	234	6	,	,	,
cord-009376-a35a92gh	234	7	and	and	CC
cord-009376-a35a92gh	234	8	while	while	IN
cord-009376-a35a92gh	234	9	antibody	antibody	NN
cord-009376-a35a92gh	234	10	to	to	IN
cord-009376-a35a92gh	234	11	the	the	DT
cord-009376-a35a92gh	234	12	virus	virus	NN
cord-009376-a35a92gh	234	13	is	be	VBZ
cord-009376-a35a92gh	234	14	lacking	lack	VBG
cord-009376-a35a92gh	234	15	in	in	IN
cord-009376-a35a92gh	234	16	the	the	DT
cord-009376-a35a92gh	234	17	general	general	JJ
cord-009376-a35a92gh	234	18	population	population	NN
cord-009376-a35a92gh	234	19	,	,	,
cord-009376-a35a92gh	234	20	71	71	CD
cord-009376-a35a92gh	234	21	%	%	NN
cord-009376-a35a92gh	234	22	of	of	IN
cord-009376-a35a92gh	234	23	veterinarians	veterinarian	NNS
cord-009376-a35a92gh	234	24	,	,	,
cord-009376-a35a92gh	234	25	50	50	CD
cord-009376-a35a92gh	234	26	%	%	NN
cord-009376-a35a92gh	234	27	of	of	IN
cord-009376-a35a92gh	234	28	cattle	cattle	NNS
cord-009376-a35a92gh	234	29	farmers	farmer	NNS
cord-009376-a35a92gh	234	30	and	and	CC
cord-009376-a35a92gh	234	31	Ž	Ž	NNP
cord-009376-a35a92gh	234	32	40	40	CD
cord-009376-a35a92gh	234	33	%	%	NN
cord-009376-a35a92gh	234	34	of	of	IN
cord-009376-a35a92gh	234	35	abattoir	abattoir	NN
cord-009376-a35a92gh	234	36	workers	worker	NNS
cord-009376-a35a92gh	234	37	are	be	VBP
cord-009376-a35a92gh	234	38	sero	sero	NN
cord-009376-a35a92gh	234	39	-	-	HYPH
cord-009376-a35a92gh	234	40	positive	positive	JJ
cord-009376-a35a92gh	234	41	Parry	Parry	NNP
cord-009376-a35a92gh	234	42	.	.	.
cord-009376-a35a92gh	235	1	and	and	CC
cord-009376-a35a92gh	235	2	Gardner	Gardner	NNP
cord-009376-a35a92gh	235	3	,	,	,
cord-009376-a35a92gh	235	4	1986	1986	CD
cord-009376-a35a92gh	235	5	.	.	.
cord-009376-a35a92gh	236	1	Detection	detection	NN
cord-009376-a35a92gh	236	2	of	of	IN
cord-009376-a35a92gh	236	3	BPyV	bpyv	JJ
cord-009376-a35a92gh	236	4	by	by	IN
cord-009376-a35a92gh	236	5	quantitative	quantitative	JJ
cord-009376-a35a92gh	236	6	PCR	PCR	NNP
cord-009376-a35a92gh	236	7	is	be	VBZ
cord-009376-a35a92gh	236	8	fully	fully	RB
cord-009376-a35a92gh	236	9	validated	validate	VBN
cord-009376-a35a92gh	236	10	to	to	IN
cord-009376-a35a92gh	236	11	ICH	ICH	NNP
cord-009376-a35a92gh	236	12	guidelines	guideline	NNS
cord-009376-a35a92gh	236	13	and	and	CC
cord-009376-a35a92gh	236	14	the	the	DT
cord-009376-a35a92gh	236	15	assay	assay	NN
cord-009376-a35a92gh	236	16	is	be	VBZ
cord-009376-a35a92gh	236	17	capable	capable	JJ
cord-009376-a35a92gh	236	18	of	of	IN
cord-009376-a35a92gh	236	19	detecting	detect	VBG
cord-009376-a35a92gh	236	20	100	100	CD
cord-009376-a35a92gh	236	21	viral	viral	JJ
cord-009376-a35a92gh	236	22	genome	genome	NN
cord-009376-a35a92gh	236	23	copies	copy	NNS
cord-009376-a35a92gh	236	24	on	on	IN
cord-009376-a35a92gh	236	25	a	a	DT
cord-009376-a35a92gh	236	26	routine	routine	JJ
cord-009376-a35a92gh	236	27	basis	basis	NN
cord-009376-a35a92gh	236	28	.	.	.
cord-009376-a35a92gh	237	1	The	the	DT
cord-009376-a35a92gh	237	2	Bunyaviridae	Bunyaviridae	NNP
cord-009376-a35a92gh	237	3	family	family	NN
cord-009376-a35a92gh	237	4	contains	contain	VBZ
cord-009376-a35a92gh	237	5	over	over	IN
cord-009376-a35a92gh	237	6	300	300	CD
cord-009376-a35a92gh	237	7	viruses	virus	NNS
cord-009376-a35a92gh	237	8	grouped	group	VBN
cord-009376-a35a92gh	237	9	into	into	IN
cord-009376-a35a92gh	237	10	five	five	CD
cord-009376-a35a92gh	237	11	genera	genus	NNS
cord-009376-a35a92gh	237	12	.	.	.
cord-009376-a35a92gh	238	1	Unlike	unlike	IN
cord-009376-a35a92gh	238	2	most	most	JJS
cord-009376-a35a92gh	238	3	other	other	JJ
cord-009376-a35a92gh	238	4	enveloped	envelop	VBN
cord-009376-a35a92gh	238	5	viruses	virus	NNS
cord-009376-a35a92gh	238	6	,	,	,
cord-009376-a35a92gh	238	7	they	-PRON-	PRP
cord-009376-a35a92gh	238	8	bud	bud	VBP
cord-009376-a35a92gh	238	9	into	into	IN
cord-009376-a35a92gh	238	10	intracytoplasmic	intracytoplasmic	JJ
cord-009376-a35a92gh	238	11	vesicles	vesicle	NNS
cord-009376-a35a92gh	238	12	associated	associate	VBN
cord-009376-a35a92gh	238	13	with	with	IN
cord-009376-a35a92gh	238	14	the	the	DT
cord-009376-a35a92gh	238	15	golgi	golgi	NN
cord-009376-a35a92gh	238	16	apparatus	apparatus	NN
cord-009376-a35a92gh	238	17	forming	form	VBG
cord-009376-a35a92gh	238	18	virions	virion	NNS
cord-009376-a35a92gh	238	19	80	80	CD
cord-009376-a35a92gh	238	20	᎐	᎐	CD
cord-009376-a35a92gh	238	21	120	120	CD
cord-009376-a35a92gh	238	22	nm	nm	CD
cord-009376-a35a92gh	238	23	in	in	IN
cord-009376-a35a92gh	238	24	diameter	diameter	NN
cord-009376-a35a92gh	238	25	.	.	.
cord-009376-a35a92gh	239	1	The	the	DT
cord-009376-a35a92gh	239	2	genome	genome	NN
cord-009376-a35a92gh	239	3	of	of	IN
cord-009376-a35a92gh	239	4	the	the	DT
cord-009376-a35a92gh	239	5	virus	virus	NN
cord-009376-a35a92gh	239	6	is	be	VBZ
cord-009376-a35a92gh	239	7	segmented	segment	VBN
cord-009376-a35a92gh	239	8	into	into	IN
cord-009376-a35a92gh	239	9	three	three	CD
cord-009376-a35a92gh	239	10	strands	strand	NNS
cord-009376-a35a92gh	239	11	permitting	permit	VBG
cord-009376-a35a92gh	239	12	complex	complex	JJ
cord-009376-a35a92gh	239	13	reassortants	reassortant	NNS
cord-009376-a35a92gh	239	14	,	,	,
cord-009376-a35a92gh	239	15	as	as	IN
cord-009376-a35a92gh	239	16	occurs	occur	VBZ
cord-009376-a35a92gh	239	17	with	with	IN
cord-009376-a35a92gh	239	18	Influenza	Influenza	NNP
cord-009376-a35a92gh	239	19	virus	virus	NN
cord-009376-a35a92gh	239	20	.	.	.
cord-009376-a35a92gh	240	1	Consequently	consequently	RB
cord-009376-a35a92gh	240	2	,	,	,
cord-009376-a35a92gh	240	3	the	the	DT
cord-009376-a35a92gh	240	4	taxonomy	taxonomy	NN
cord-009376-a35a92gh	240	5	of	of	IN
cord-009376-a35a92gh	240	6	these	these	DT
cord-009376-a35a92gh	240	7	viruses	virus	NNS
cord-009376-a35a92gh	240	8	can	can	MD
cord-009376-a35a92gh	240	9	be	be	VB
cord-009376-a35a92gh	240	10	complex	complex	JJ
cord-009376-a35a92gh	240	11	ŽLundstrom	ŽLundstrom	NNP
cord-009376-a35a92gh	240	12	,	,	,
cord-009376-a35a92gh	240	13	1999	1999	CD
cord-009376-a35a92gh	240	14	;	;	:
cord-009376-a35a92gh	240	15	Borucki	Borucki	NNP
cord-009376-a35a92gh	240	16	et	et	NNP
cord-009376-a35a92gh	240	17	al	al	NNP
cord-009376-a35a92gh	240	18	.	.	.
cord-009376-a35a92gh	241	1	,	,	,
cord-009376-a35a92gh	241	2	1999	1999	CD
cord-009376-a35a92gh	241	3	;	;	:
cord-009376-a35a92gh	241	4	Mahy	Mahy	NNP
cord-009376-a35a92gh	241	5	,	,	,
cord-009376-a35a92gh	241	6	.	.	.
cord-009376-a35a92gh	242	1	1998	1998	CD
cord-009376-a35a92gh	242	2	.	.	.
cord-009376-a35a92gh	243	1	Within	within	IN
cord-009376-a35a92gh	243	2	the	the	DT
cord-009376-a35a92gh	243	3	Bunyavirus	Bunyavirus	NNP
cord-009376-a35a92gh	243	4	genus	genus	NN
cord-009376-a35a92gh	243	5	,	,	,
cord-009376-a35a92gh	243	6	the	the	DT
cord-009376-a35a92gh	243	7	most	most	RBS
cord-009376-a35a92gh	243	8	relevant	relevant	JJ
cord-009376-a35a92gh	243	9	serogroups	serogroup	NNS
cord-009376-a35a92gh	243	10	or	or	CC
cord-009376-a35a92gh	243	11	genetic	genetic	JJ
cord-009376-a35a92gh	243	12	complexes	complex	NNS
cord-009376-a35a92gh	243	13	are	be	VBP
cord-009376-a35a92gh	243	14	the	the	DT
cord-009376-a35a92gh	243	15	Californian	californian	JJ
cord-009376-a35a92gh	243	16	serogroup	serogroup	NN
cord-009376-a35a92gh	243	17	and	and	CC
cord-009376-a35a92gh	243	18	the	the	DT
cord-009376-a35a92gh	243	19	Bunyamwera	Bunyamwera	NNP
cord-009376-a35a92gh	243	20	serogroup	serogroup	NN
cord-009376-a35a92gh	243	21	.	.	.
cord-009376-a35a92gh	244	1	Within	within	IN
cord-009376-a35a92gh	244	2	the	the	DT
cord-009376-a35a92gh	244	3	Bunyamwera	Bunyamwera	NNP
cord-009376-a35a92gh	244	4	group	group	NN
cord-009376-a35a92gh	244	5	,	,	,
cord-009376-a35a92gh	244	6	the	the	DT
cord-009376-a35a92gh	244	7	Cache	cache	NN
cord-009376-a35a92gh	244	8	Ž	Ž	NNP
cord-009376-a35a92gh	244	9	.	.	.
cord-009376-a35a92gh	245	1	valley	valley	NNP
cord-009376-a35a92gh	245	2	virus	virus	NN
cord-009376-a35a92gh	245	3	CVV	CVV	NNP
cord-009376-a35a92gh	245	4	is	be	VBZ
cord-009376-a35a92gh	245	5	a	a	DT
cord-009376-a35a92gh	245	6	recognised	recognise	VBN
cord-009376-a35a92gh	245	7	infection	infection	NN
cord-009376-a35a92gh	245	8	of	of	IN
cord-009376-a35a92gh	245	9	sheep	sheep	NNS
cord-009376-a35a92gh	245	10	and	and	CC
cord-009376-a35a92gh	245	11	cattle	cattle	NNS
cord-009376-a35a92gh	245	12	.	.	.
cord-009376-a35a92gh	246	1	CVV	CVV	NNP
cord-009376-a35a92gh	246	2	can	can	MD
cord-009376-a35a92gh	246	3	be	be	VB
cord-009376-a35a92gh	246	4	transmitted	transmit	VBN
cord-009376-a35a92gh	246	5	congenitally	congenitally	RB
cord-009376-a35a92gh	246	6	and	and	CC
cord-009376-a35a92gh	246	7	infection	infection	NN
cord-009376-a35a92gh	246	8	of	of	IN
cord-009376-a35a92gh	246	9	foetal	foetal	JJ
cord-009376-a35a92gh	246	10	calf	calf	NN
cord-009376-a35a92gh	246	11	serum	serum	NN
cord-009376-a35a92gh	246	12	is	be	VBZ
cord-009376-a35a92gh	246	13	Ž	ž	NN
cord-009376-a35a92gh	246	14	possible	possible	JJ
cord-009376-a35a92gh	247	1	McLean	McLean	NNP
cord-009376-a35a92gh	247	2	et	et	NNP
cord-009376-a35a92gh	247	3	al	al	NNP
cord-009376-a35a92gh	247	4	.	.	NNP
cord-009376-a35a92gh	247	5	,	,	,
cord-009376-a35a92gh	247	6	1996	1996	CD
cord-009376-a35a92gh	247	7	;	;	:
cord-009376-a35a92gh	247	8	Blackmore	Blackmore	NNP
cord-009376-a35a92gh	247	9	and	and	CC
cord-009376-a35a92gh	247	10	Grimstad	Grimstad	NNP
cord-009376-a35a92gh	247	11	,	,	,
cord-009376-a35a92gh	247	12	1998	1998	CD
cord-009376-a35a92gh	247	13	;	;	:
cord-009376-a35a92gh	247	14	Edwards	Edwards	NNP
cord-009376-a35a92gh	247	15	et	et	NNP
cord-009376-a35a92gh	247	16	al	al	NNP
cord-009376-a35a92gh	247	17	.	.	NNP
cord-009376-a35a92gh	247	18	,	,	,
cord-009376-a35a92gh	247	19	1997	1997	CD
cord-009376-a35a92gh	247	20	;	;	:
cord-009376-a35a92gh	247	21	Chung	Chung	NNP
cord-009376-a35a92gh	247	22	et	et	NNP
cord-009376-a35a92gh	247	23	.	.	.
cord-009376-a35a92gh	248	1	al	al	NNP
cord-009376-a35a92gh	248	2	.	.	NNP
cord-009376-a35a92gh	248	3	,	,	,
cord-009376-a35a92gh	248	4	1991	1991	CD
cord-009376-a35a92gh	248	5	,	,	,
cord-009376-a35a92gh	248	6	emphasising	emphasise	VBG
cord-009376-a35a92gh	248	7	that	that	IN
cord-009376-a35a92gh	248	8	CVV	CVV	NNP
cord-009376-a35a92gh	248	9	has	have	VBZ
cord-009376-a35a92gh	248	10	the	the	DT
cord-009376-a35a92gh	248	11	potential	potential	NN
cord-009376-a35a92gh	248	12	to	to	TO
cord-009376-a35a92gh	248	13	be	be	VB
cord-009376-a35a92gh	248	14	problematic	problematic	JJ
cord-009376-a35a92gh	248	15	in	in	IN
cord-009376-a35a92gh	248	16	large	large	JJ
cord-009376-a35a92gh	248	17	scale	scale	NN
cord-009376-a35a92gh	248	18	fermentation	fermentation	NN
cord-009376-a35a92gh	248	19	culture	culture	NN
cord-009376-a35a92gh	248	20	during	during	IN
cord-009376-a35a92gh	248	21	manufacturing	manufacturing	NN
cord-009376-a35a92gh	248	22	.	.	.
cord-009376-a35a92gh	249	1	CVV	CVV	NNP
cord-009376-a35a92gh	249	2	is	be	VBZ
cord-009376-a35a92gh	249	3	lytic	lytic	JJ
cord-009376-a35a92gh	249	4	to	to	IN
cord-009376-a35a92gh	249	5	cell	cell	NN
cord-009376-a35a92gh	249	6	lines	line	NNS
cord-009376-a35a92gh	249	7	including	include	VBG
cord-009376-a35a92gh	249	8	,	,	,
cord-009376-a35a92gh	249	9	CHO	CHO	NNP
cord-009376-a35a92gh	249	10	-	-	HYPH
cord-009376-a35a92gh	249	11	K1	K1	NNP
cord-009376-a35a92gh	249	12	and	and	CC
cord-009376-a35a92gh	249	13	Vero	Vero	NNP
cord-009376-a35a92gh	249	14	,	,	,
cord-009376-a35a92gh	249	15	and	and	CC
cord-009376-a35a92gh	249	16	therefore	therefore	RB
cord-009376-a35a92gh	249	17	can	can	MD
cord-009376-a35a92gh	249	18	also	also	RB
cord-009376-a35a92gh	249	19	be	be	VB
cord-009376-a35a92gh	249	20	detected	detect	VBN
cord-009376-a35a92gh	249	21	by	by	IN
cord-009376-a35a92gh	249	22	culture	culture	NN
cord-009376-a35a92gh	249	23	-	-	HYPH
cord-009376-a35a92gh	249	24	based	base	VBN
cord-009376-a35a92gh	249	25	bio	bio	NN
cord-009376-a35a92gh	249	26	-	-	HYPH
cord-009376-a35a92gh	249	27	assays	assays	JJ
cord-009376-a35a92gh	249	28	Ž	ž	NN
cord-009376-a35a92gh	249	29	.	.	.
cord-009376-a35a92gh	250	1	Edwards	Edwards	NNP
cord-009376-a35a92gh	250	2	et	et	NNP
cord-009376-a35a92gh	250	3	al	al	NNP
cord-009376-a35a92gh	250	4	.	.	NNP
cord-009376-a35a92gh	250	5	,	,	,
cord-009376-a35a92gh	250	6	1997	1997	CD
cord-009376-a35a92gh	250	7	.	.	.
cord-009376-a35a92gh	251	1	Since	since	IN
cord-009376-a35a92gh	251	2	CVV	CVV	NNP
cord-009376-a35a92gh	251	3	is	be	VBZ
cord-009376-a35a92gh	251	4	suspected	suspect	VBN
cord-009376-a35a92gh	251	5	to	to	TO
cord-009376-a35a92gh	251	6	cause	cause	VB
cord-009376-a35a92gh	251	7	foetal	foetal	JJ
cord-009376-a35a92gh	251	8	malformations	malformation	NNS
cord-009376-a35a92gh	251	9	in	in	IN
cord-009376-a35a92gh	251	10	cattle	cattle	NNS
cord-009376-a35a92gh	251	11	the	the	DT
cord-009376-a35a92gh	251	12	presence	presence	NN
cord-009376-a35a92gh	251	13	of	of	IN
cord-009376-a35a92gh	251	14	this	this	DT
cord-009376-a35a92gh	251	15	virus	virus	NN
cord-009376-a35a92gh	251	16	in	in	IN
cord-009376-a35a92gh	251	17	serum	serum	NN
cord-009376-a35a92gh	251	18	represents	represent	VBZ
cord-009376-a35a92gh	251	19	a	a	DT
cord-009376-a35a92gh	251	20	major	major	JJ
cord-009376-a35a92gh	251	21	safety	safety	NN
cord-009376-a35a92gh	251	22	Ž	ž	NN
cord-009376-a35a92gh	251	23	.	.	.
cord-009376-a35a92gh	252	1	concern	concern	NN
cord-009376-a35a92gh	252	2	Edwards	Edwards	NNP
cord-009376-a35a92gh	252	3	et	et	NNP
cord-009376-a35a92gh	252	4	al	al	NNP
cord-009376-a35a92gh	252	5	.	.	NNP
cord-009376-a35a92gh	252	6	,	,	,
cord-009376-a35a92gh	252	7	1997	1997	CD
cord-009376-a35a92gh	252	8	.	.	.
cord-009376-a35a92gh	253	1	Detection	detection	NN
cord-009376-a35a92gh	253	2	of	of	IN
cord-009376-a35a92gh	253	3	CVV	CVV	NNP
cord-009376-a35a92gh	253	4	by	by	IN
cord-009376-a35a92gh	253	5	quantitative	quantitative	JJ
cord-009376-a35a92gh	253	6	RT	RT	NNP
cord-009376-a35a92gh	253	7	-	-	HYPH
cord-009376-a35a92gh	253	8	PCR	PCR	NNP
cord-009376-a35a92gh	253	9	is	be	VBZ
cord-009376-a35a92gh	253	10	fully	fully	RB
cord-009376-a35a92gh	253	11	validated	validate	VBN
cord-009376-a35a92gh	253	12	to	to	IN
cord-009376-a35a92gh	253	13	ICH	ICH	NNP
cord-009376-a35a92gh	253	14	guidelines	guideline	NNS
cord-009376-a35a92gh	253	15	and	and	CC
cord-009376-a35a92gh	253	16	the	the	DT
cord-009376-a35a92gh	253	17	assay	assay	NN
cord-009376-a35a92gh	253	18	is	be	VBZ
cord-009376-a35a92gh	253	19	capable	capable	JJ
cord-009376-a35a92gh	253	20	of	of	IN
cord-009376-a35a92gh	253	21	detecting	detect	VBG
cord-009376-a35a92gh	253	22	100	100	CD
cord-009376-a35a92gh	253	23	viral	viral	JJ
cord-009376-a35a92gh	253	24	RNA	RNA	NNP
cord-009376-a35a92gh	253	25	copies	copy	NNS
cord-009376-a35a92gh	253	26	on	on	IN
cord-009376-a35a92gh	253	27	a	a	DT
cord-009376-a35a92gh	253	28	routine	routine	JJ
cord-009376-a35a92gh	253	29	basis	basis	NN
cord-009376-a35a92gh	253	30	.	.	.
cord-009376-a35a92gh	254	1	The	the	DT
cord-009376-a35a92gh	254	2	95	95	CD
cord-009376-a35a92gh	254	3	%	%	NN
cord-009376-a35a92gh	254	4	confidence	confidence	NN
cord-009376-a35a92gh	254	5	interval	interval	NN
cord-009376-a35a92gh	254	6	of	of	IN
cord-009376-a35a92gh	254	7	C	c	NN
cord-009376-a35a92gh	254	8	values	value	NNS
cord-009376-a35a92gh	254	9	produced	produce	VBN
cord-009376-a35a92gh	254	10	by	by	IN
cord-009376-a35a92gh	254	11	100	100	CD
cord-009376-a35a92gh	254	12	T	T	NNP
cord-009376-a35a92gh	254	13	viral	viral	JJ
cord-009376-a35a92gh	254	14	copies	copy	NNS
cord-009376-a35a92gh	254	15	per	per	IN
cord-009376-a35a92gh	254	16	100	100	CD
cord-009376-a35a92gh	254	17	ng	ng	NN
cord-009376-a35a92gh	254	18	of	of	IN
cord-009376-a35a92gh	254	19	non	non	JJ
cord-009376-a35a92gh	254	20	-	-	JJ
cord-009376-a35a92gh	254	21	infected	infected	JJ
cord-009376-a35a92gh	254	22	cellular	cellular	JJ
cord-009376-a35a92gh	254	23	RNA	RNA	NNP
cord-009376-a35a92gh	254	24	was	be	VBD
cord-009376-a35a92gh	254	25	significantly	significantly	RB
cord-009376-a35a92gh	254	26	different	different	JJ
cord-009376-a35a92gh	254	27	from	from	IN
cord-009376-a35a92gh	254	28	the	the	DT
cord-009376-a35a92gh	254	29	negative	negative	JJ
cord-009376-a35a92gh	254	30	Ž	ž	NN
cord-009376-a35a92gh	254	31	.	.	.
cord-009376-a35a92gh	255	1	control	control	NNP
cord-009376-a35a92gh	255	2	RNA	RNA	NNP
cord-009376-a35a92gh	255	3	using	use	VBG
cord-009376-a35a92gh	255	4	two	two	CD
cord-009376-a35a92gh	255	5	operators	operator	NNS
cord-009376-a35a92gh	255	6	 	 	_SP
cord-009376-a35a92gh	255	7	Circoviruses	Circoviruses	NNPS
cord-009376-a35a92gh	255	8	are	be	VBP
cord-009376-a35a92gh	255	9	amongst	amongst	IN
cord-009376-a35a92gh	255	10	the	the	DT
cord-009376-a35a92gh	255	11	smallest	small	JJS
cord-009376-a35a92gh	255	12	mammalian	mammalian	JJ
cord-009376-a35a92gh	255	13	viruses	virus	NNS
cord-009376-a35a92gh	255	14	at	at	IN
cord-009376-a35a92gh	255	15	approximately	approximately	RB
cord-009376-a35a92gh	255	16	17	17	CD
cord-009376-a35a92gh	255	17	nm	nm	NN
cord-009376-a35a92gh	255	18	,	,	,
cord-009376-a35a92gh	255	19	and	and	CC
cord-009376-a35a92gh	255	20	like	like	IN
cord-009376-a35a92gh	255	21	parvoviruses	parvovirus	NNS
cord-009376-a35a92gh	255	22	,	,	,
cord-009376-a35a92gh	255	23	are	be	VBP
cord-009376-a35a92gh	255	24	highly	highly	RB
cord-009376-a35a92gh	255	25	resistant	resistant	JJ
cord-009376-a35a92gh	255	26	in	in	IN
cord-009376-a35a92gh	255	27	the	the	DT
cord-009376-a35a92gh	255	28	environ-	environ-	NN
cord-009376-a35a92gh	255	29	Ž	Ž	NNP
cord-009376-a35a92gh	255	30	.	.	.
cord-009376-a35a92gh	256	1	y	y	NNP
cord-009376-a35a92gh	256	2	q	q	NNP
cord-009376-a35a92gh	256	3	(	(	-LRB-
cord-009376-a35a92gh	256	4	)	)	-RRB-
cord-009376-a35a92gh	256	5	Ž	ž	NN
cord-009376-a35a92gh	256	6	.	.	.
cord-009376-a35a92gh	257	1	ment	ment	NNP
cord-009376-a35a92gh	257	2	.	.	.
cord-009376-a35a92gh	258	1	Porcine	porcine	JJ
cord-009376-a35a92gh	258	2	circovirus	circovirus	JJ
cord-009376-a35a92gh	258	3	type	type	NN
cord-009376-a35a92gh	258	4	2	2	CD
cord-009376-a35a92gh	259	1	PCV2	PCV2	NNP
cord-009376-a35a92gh	259	2	has	have	VBZ
cord-009376-a35a92gh	259	3	a	a	DT
cord-009376-a35a92gh	259	4	nucleotide	nucleotide	JJ
cord-009376-a35a92gh	259	5	sequence	sequence	NN
cord-009376-a35a92gh	259	6	homology	homology	NN
cord-009376-a35a92gh	259	7	to	to	IN
cord-009376-a35a92gh	259	8	Bovine	bovine	JJ
cord-009376-a35a92gh	259	9	cir	cir	NN
cord-009376-a35a92gh	259	10	-	-	HYPH
cord-009376-a35a92gh	259	11	Ž	Ž	NNP
cord-009376-a35a92gh	259	12	.	.	.
cord-009376-a35a92gh	260	1	covirus	covirus	NNP
cord-009376-a35a92gh	260	2	BCV	BCV	NNP
cord-009376-a35a92gh	260	3	and	and	CC
cord-009376-a35a92gh	260	4	has	have	VBZ
cord-009376-a35a92gh	260	5	been	be	VBN
cord-009376-a35a92gh	260	6	shown	show	VBN
cord-009376-a35a92gh	260	7	to	to	TO
cord-009376-a35a92gh	260	8	be	be	VB
cord-009376-a35a92gh	260	9	involved	involve	VBN
cord-009376-a35a92gh	260	10	in	in	IN
cord-009376-a35a92gh	260	11	the	the	DT
cord-009376-a35a92gh	260	12	formations	formation	NNS
cord-009376-a35a92gh	260	13	of	of	IN
cord-009376-a35a92gh	260	14	organ	organ	NN
cord-009376-a35a92gh	260	15	lesions	lesion	NNS
cord-009376-a35a92gh	260	16	in	in	IN
cord-009376-a35a92gh	260	17	pigs	pig	NNS
cord-009376-a35a92gh	260	18	.	.	.
cord-009376-a35a92gh	261	1	The	the	DT
cord-009376-a35a92gh	261	2	close	close	JJ
cord-009376-a35a92gh	261	3	relationship	relationship	NN
cord-009376-a35a92gh	261	4	between	between	IN
cord-009376-a35a92gh	261	5	these	these	DT
cord-009376-a35a92gh	261	6	two	two	CD
cord-009376-a35a92gh	261	7	viruses	virus	NNS
cord-009376-a35a92gh	261	8	has	have	VBZ
cord-009376-a35a92gh	261	9	led	lead	VBN
cord-009376-a35a92gh	261	10	to	to	IN
cord-009376-a35a92gh	261	11	suggestions	suggestion	NNS
cord-009376-a35a92gh	261	12	that	that	IN
cord-009376-a35a92gh	261	13	this	this	DT
cord-009376-a35a92gh	261	14	group	group	NN
cord-009376-a35a92gh	261	15	of	of	IN
cord-009376-a35a92gh	261	16	viruses	virus	NNS
cord-009376-a35a92gh	261	17	may	may	MD
cord-009376-a35a92gh	261	18	cross	cross	VB
cord-009376-a35a92gh	261	19	species	species	NN
cord-009376-a35a92gh	261	20	barriers	barrier	NNS
cord-009376-a35a92gh	261	21	.	.	.
cord-009376-a35a92gh	262	1	In	in	IN
cord-009376-a35a92gh	262	2	some	some	DT
cord-009376-a35a92gh	262	3	cases	case	NNS
cord-009376-a35a92gh	262	4	,	,	,
cord-009376-a35a92gh	262	5	circoviruses	circoviruse	NNS
cord-009376-a35a92gh	262	6	can	can	MD
cord-009376-a35a92gh	262	7	remain	remain	VB
cord-009376-a35a92gh	262	8	as	as	RB
cord-009376-a35a92gh	262	9	latent	latent	JJ
cord-009376-a35a92gh	262	10	and	and	CC
cord-009376-a35a92gh	262	11	persist	persist	VB
cord-009376-a35a92gh	262	12	as	as	IN
cord-009376-a35a92gh	262	13	non	non	JJ
cord-009376-a35a92gh	262	14	-	-	JJ
cord-009376-a35a92gh	262	15	cytopathic	cytopathic	JJ
cord-009376-a35a92gh	262	16	Ž	ž	NN
cord-009376-a35a92gh	262	17	.	.	.
cord-009376-a35a92gh	263	1	infections	infection	NNS
cord-009376-a35a92gh	263	2	in	in	IN
cord-009376-a35a92gh	263	3	vitro	vitro	FW
cord-009376-a35a92gh	263	4	Allan	Allan	NNP
cord-009376-a35a92gh	263	5	and	and	CC
cord-009376-a35a92gh	263	6	Ellis	Ellis	NNP
cord-009376-a35a92gh	263	7	,	,	,
cord-009376-a35a92gh	263	8	2000	2000	CD
cord-009376-a35a92gh	263	9	.	.	.
cord-009376-a35a92gh	264	1	Techniques	technique	NNS
cord-009376-a35a92gh	264	2	currently	currently	RB
cord-009376-a35a92gh	264	3	available	available	JJ
cord-009376-a35a92gh	264	4	for	for	IN
cord-009376-a35a92gh	264	5	detection	detection	NN
cord-009376-a35a92gh	264	6	of	of	IN
cord-009376-a35a92gh	264	7	all	all	DT
cord-009376-a35a92gh	264	8	forms	form	NNS
cord-009376-a35a92gh	264	9	of	of	IN
cord-009376-a35a92gh	264	10	circovirus	circovirus	NNP
cord-009376-a35a92gh	264	11	are	be	VBP
cord-009376-a35a92gh	264	12	limited	limit	VBN
cord-009376-a35a92gh	264	13	,	,	,
cord-009376-a35a92gh	264	14	and	and	CC
cord-009376-a35a92gh	264	15	there	there	EX
cord-009376-a35a92gh	264	16	is	be	VBZ
cord-009376-a35a92gh	264	17	no	no	DT
cord-009376-a35a92gh	264	18	current	current	JJ
cord-009376-a35a92gh	264	19	test	test	NN
cord-009376-a35a92gh	264	20	that	that	WDT
cord-009376-a35a92gh	264	21	differentiates	differentiate	VBZ
cord-009376-a35a92gh	264	22	between	between	IN
cord-009376-a35a92gh	264	23	infectious	infectious	JJ
cord-009376-a35a92gh	264	24	virus	virus	NN
cord-009376-a35a92gh	264	25	and	and	CC
cord-009376-a35a92gh	264	26	nucleic	nucleic	JJ
cord-009376-a35a92gh	264	27	acids	acid	NNS
cord-009376-a35a92gh	264	28	.	.	.
cord-009376-a35a92gh	265	1	Consequently	consequently	RB
cord-009376-a35a92gh	265	2	,	,	,
cord-009376-a35a92gh	265	3	specific	specific	JJ
cord-009376-a35a92gh	265	4	Q	Q	NNP
cord-009376-a35a92gh	265	5	-	-	HYPH
cord-009376-a35a92gh	265	6	PCR	pcr	NN
cord-009376-a35a92gh	265	7	assays	assay	NNS
cord-009376-a35a92gh	265	8	are	be	VBP
cord-009376-a35a92gh	265	9	required	require	VBN
cord-009376-a35a92gh	265	10	to	to	TO
cord-009376-a35a92gh	265	11	detect	detect	VB
cord-009376-a35a92gh	265	12	these	these	DT
cord-009376-a35a92gh	265	13	viruses	virus	NNS
cord-009376-a35a92gh	265	14	.	.	.
cord-009376-a35a92gh	266	1	As	as	IN
cord-009376-a35a92gh	266	2	shown	show	VBN
cord-009376-a35a92gh	266	3	in	in	IN
cord-009376-a35a92gh	266	4	Fig	Fig	NNP
cord-009376-a35a92gh	266	5	.	.	.
cord-009376-a35a92gh	267	1	3	3	CD
cord-009376-a35a92gh	267	2	,	,	,
cord-009376-a35a92gh	267	3	multiple	multiple	JJ
cord-009376-a35a92gh	267	4	sequence	sequence	NN
cord-009376-a35a92gh	267	5	alignment	alignment	NN
cord-009376-a35a92gh	267	6	of	of	IN
cord-009376-a35a92gh	267	7	the	the	DT
cord-009376-a35a92gh	267	8	Bovine	bovine	JJ
cord-009376-a35a92gh	267	9	circovirus	circovirus	NN
cord-009376-a35a92gh	267	10	and	and	CC
cord-009376-a35a92gh	267	11	Porcine	porcine	JJ
cord-009376-a35a92gh	267	12	circovirus	circovirus	JJ
cord-009376-a35a92gh	267	13	genomes	genome	NNS
cord-009376-a35a92gh	267	14	has	have	VBZ
cord-009376-a35a92gh	267	15	identified	identify	VBN
cord-009376-a35a92gh	267	16	the	the	DT
cord-009376-a35a92gh	267	17	Q	Q	NNP
cord-009376-a35a92gh	267	18	-	-	HYPH
cord-009376-a35a92gh	267	19	PCR	PCR	NNP
cord-009376-a35a92gh	267	20	primers	primer	NNS
cord-009376-a35a92gh	267	21	and	and	CC
cord-009376-a35a92gh	267	22	probe	probe	NN
cord-009376-a35a92gh	267	23	that	that	WDT
cord-009376-a35a92gh	267	24	will	will	MD
cord-009376-a35a92gh	267	25	detect	detect	VB
cord-009376-a35a92gh	267	26	a	a	DT
cord-009376-a35a92gh	267	27	range	range	NN
cord-009376-a35a92gh	267	28	of	of	IN
cord-009376-a35a92gh	267	29	strains	strain	NNS
cord-009376-a35a92gh	267	30	isolated	isolate	VBN
cord-009376-a35a92gh	267	31	from	from	IN
cord-009376-a35a92gh	267	32	many	many	JJ
cord-009376-a35a92gh	267	33	global	global	JJ
cord-009376-a35a92gh	267	34	locations	location	NNS
cord-009376-a35a92gh	267	35	.	.	.
cord-009376-a35a92gh	268	1	The	the	DT
cord-009376-a35a92gh	268	2	Bovine	bovine	JJ
cord-009376-a35a92gh	268	3	circovirus	circovirus	NN
cord-009376-a35a92gh	268	4	and	and	CC
cord-009376-a35a92gh	268	5	Porcine	porcine	JJ
cord-009376-a35a92gh	268	6	circovirus	circovirus	NN
cord-009376-a35a92gh	268	7	Q	Q	NNP
cord-009376-a35a92gh	268	8	-	-	HYPH
cord-009376-a35a92gh	268	9	PCR	PCR	NNP
cord-009376-a35a92gh	268	10	is	be	VBZ
cord-009376-a35a92gh	268	11	fully	fully	RB
cord-009376-a35a92gh	268	12	validated	validate	VBN
cord-009376-a35a92gh	268	13	to	to	IN
cord-009376-a35a92gh	268	14	ICH	ICH	NNP
cord-009376-a35a92gh	268	15	guidelines	guideline	NNS
cord-009376-a35a92gh	268	16	and	and	CC
cord-009376-a35a92gh	268	17	can	can	MD
cord-009376-a35a92gh	268	18	routinely	routinely	RB
cord-009376-a35a92gh	268	19	detect	detect	VB
cord-009376-a35a92gh	268	20	100	100	CD
cord-009376-a35a92gh	268	21	genome	genome	NN
cord-009376-a35a92gh	268	22	copies	copy	NNS
cord-009376-a35a92gh	268	23	.	.	.
cord-009376-a35a92gh	269	1	In	in	IN
cord-009376-a35a92gh	269	2	summary	summary	NN
cord-009376-a35a92gh	269	3	,	,	,
cord-009376-a35a92gh	269	4	CVV	CVV	NNP
cord-009376-a35a92gh	269	5	,	,	,
cord-009376-a35a92gh	269	6	BPyV	bpyv	JJ
cord-009376-a35a92gh	269	7	,	,	,
cord-009376-a35a92gh	269	8	Porcine	porcine	JJ
cord-009376-a35a92gh	269	9	circovirus	circovirus	NN
cord-009376-a35a92gh	269	10	and	and	CC
cord-009376-a35a92gh	269	11	Bovine	bovine	JJ
cord-009376-a35a92gh	269	12	circovirus	circovirus	NNP
cord-009376-a35a92gh	269	13	represent	represent	VBP
cord-009376-a35a92gh	269	14	a	a	DT
cord-009376-a35a92gh	269	15	new	new	JJ
cord-009376-a35a92gh	269	16	threat	threat	NN
cord-009376-a35a92gh	269	17	to	to	IN
cord-009376-a35a92gh	269	18	biotechnology	biotechnology	NN
cord-009376-a35a92gh	269	19	products	product	NNS
cord-009376-a35a92gh	269	20	and	and	CC
cord-009376-a35a92gh	269	21	steps	step	NNS
cord-009376-a35a92gh	269	22	need	need	VBP
cord-009376-a35a92gh	269	23	to	to	TO
cord-009376-a35a92gh	269	24	be	be	VB
cord-009376-a35a92gh	269	25	incorporated	incorporate	VBN
cord-009376-a35a92gh	269	26	to	to	TO
cord-009376-a35a92gh	269	27	monitor	monitor	VB
cord-009376-a35a92gh	269	28	these	these	DT
cord-009376-a35a92gh	269	29	viruses	virus	NNS
cord-009376-a35a92gh	269	30	.	.	.
cord-009376-a35a92gh	270	1	Appropriate	appropriate	JJ
cord-009376-a35a92gh	270	2	sub	sub	JJ
cord-009376-a35a92gh	270	3	-	-	JJ
cord-009376-a35a92gh	270	4	pool	pool	JJ
cord-009376-a35a92gh	270	5	testing	testing	NN
cord-009376-a35a92gh	270	6	of	of	IN
cord-009376-a35a92gh	270	7	foetal	foetal	JJ
cord-009376-a35a92gh	270	8	calf	calf	NN
cord-009376-a35a92gh	270	9	serum	serum	NN
cord-009376-a35a92gh	270	10	and	and	CC
cord-009376-a35a92gh	270	11	the	the	DT
cord-009376-a35a92gh	270	12	testing	testing	NN
cord-009376-a35a92gh	270	13	of	of	IN
cord-009376-a35a92gh	270	14	other	other	JJ
cord-009376-a35a92gh	270	15	raw	raw	JJ
cord-009376-a35a92gh	270	16	materials	material	NNS
cord-009376-a35a92gh	270	17	should	should	MD
cord-009376-a35a92gh	270	18	be	be	VB
cord-009376-a35a92gh	270	19	conducted	conduct	VBN
cord-009376-a35a92gh	270	20	.	.	.
cord-009376-a35a92gh	271	1	Since	since	IN
cord-009376-a35a92gh	271	2	it	-PRON-	PRP
cord-009376-a35a92gh	271	3	is	be	VBZ
cord-009376-a35a92gh	271	4	possible	possible	JJ
cord-009376-a35a92gh	271	5	that	that	IN
cord-009376-a35a92gh	271	6	effects	effect	NNS
cord-009376-a35a92gh	271	7	on	on	IN
cord-009376-a35a92gh	271	8	cell	cell	NN
cord-009376-a35a92gh	271	9	viability	viability	NN
cord-009376-a35a92gh	271	10	may	may	MD
cord-009376-a35a92gh	271	11	not	not	RB
cord-009376-a35a92gh	271	12	be	be	VB
cord-009376-a35a92gh	271	13	noted	note	VBN
cord-009376-a35a92gh	271	14	,	,	,
cord-009376-a35a92gh	271	15	appropriate	appropriate	JJ
cord-009376-a35a92gh	271	16	bulk	bulk	JJ
cord-009376-a35a92gh	271	17	harvest	harvest	NN
cord-009376-a35a92gh	271	18	screening	screening	NN
cord-009376-a35a92gh	271	19	of	of	IN
cord-009376-a35a92gh	271	20	cultures	culture	NNS
cord-009376-a35a92gh	271	21	should	should	MD
cord-009376-a35a92gh	271	22	be	be	VB
cord-009376-a35a92gh	271	23	undertaken	undertake	VBN
cord-009376-a35a92gh	271	24	to	to	TO
cord-009376-a35a92gh	271	25	exclude	exclude	VB
cord-009376-a35a92gh	271	26	the	the	DT
cord-009376-a35a92gh	271	27	presence	presence	NN
cord-009376-a35a92gh	271	28	of	of	IN
cord-009376-a35a92gh	271	29	these	these	DT
cord-009376-a35a92gh	271	30	viruses	virus	NNS
cord-009376-a35a92gh	271	31	,	,	,
cord-009376-a35a92gh	271	32	thereby	thereby	RB
cord-009376-a35a92gh	271	33	avoiding	avoid	VBG
cord-009376-a35a92gh	271	34	expensive	expensive	JJ
cord-009376-a35a92gh	271	35	contamination	contamination	NN
cord-009376-a35a92gh	271	36	of	of	IN
cord-009376-a35a92gh	271	37	the	the	DT
cord-009376-a35a92gh	271	38	downstream	downstream	JJ
cord-009376-a35a92gh	271	39	purification	purification	NN
cord-009376-a35a92gh	271	40	train	train	NN
cord-009376-a35a92gh	271	41	.	.	.
cord-009376-a35a92gh	272	1	Process	process	NN
cord-009376-a35a92gh	272	2	validation	validation	NN
cord-009376-a35a92gh	272	3	studies	study	NNS
cord-009376-a35a92gh	272	4	are	be	VBP
cord-009376-a35a92gh	272	5	usually	usually	RB
cord-009376-a35a92gh	272	6	performed	perform	VBN
cord-009376-a35a92gh	272	7	using	use	VBG
cord-009376-a35a92gh	272	8	a	a	DT
cord-009376-a35a92gh	272	9	downscale	downscale	NN
cord-009376-a35a92gh	272	10	of	of	IN
cord-009376-a35a92gh	272	11	the	the	DT
cord-009376-a35a92gh	272	12	manufacturer	manufacturer	NN
cord-009376-a35a92gh	272	13	's	's	POS
cord-009376-a35a92gh	272	14	purification	purification	NN
cord-009376-a35a92gh	272	15	process	process	NN
cord-009376-a35a92gh	272	16	.	.	.
cord-009376-a35a92gh	273	1	This	this	DT
cord-009376-a35a92gh	273	2	allows	allow	VBZ
cord-009376-a35a92gh	273	3	the	the	DT
cord-009376-a35a92gh	273	4	assessment	assessment	NN
cord-009376-a35a92gh	273	5	of	of	IN
cord-009376-a35a92gh	273	6	the	the	DT
cord-009376-a35a92gh	273	7	capacity	capacity	NN
cord-009376-a35a92gh	273	8	and	and	CC
cord-009376-a35a92gh	273	9	efficiency	efficiency	NN
cord-009376-a35a92gh	273	10	of	of	IN
cord-009376-a35a92gh	273	11	the	the	DT
cord-009376-a35a92gh	273	12	process	process	NN
cord-009376-a35a92gh	273	13	to	to	TO
cord-009376-a35a92gh	273	14	remove	remove	VB
cord-009376-a35a92gh	273	15	and	and	CC
cord-009376-a35a92gh	273	16	inactivate	inactivate	VB
cord-009376-a35a92gh	273	17	viruses	virus	NNS
cord-009376-a35a92gh	273	18	,	,	,
cord-009376-a35a92gh	273	19	DNA	dna	NN
cord-009376-a35a92gh	273	20	and	and	CC
cord-009376-a35a92gh	273	21	other	other	JJ
cord-009376-a35a92gh	273	22	contaminants	contaminant	NNS
cord-009376-a35a92gh	273	23	.	.	.
cord-009376-a35a92gh	274	1	All	all	DT
cord-009376-a35a92gh	274	2	manufacturers	manufacturer	NNS
cord-009376-a35a92gh	274	3	producing	produce	VBG
cord-009376-a35a92gh	274	4	recombinant	recombinant	JJ
cord-009376-a35a92gh	274	5	proteins	protein	NNS
cord-009376-a35a92gh	274	6	from	from	IN
cord-009376-a35a92gh	274	7	rodent	rodent	NN
cord-009376-a35a92gh	274	8	cell	cell	NN
cord-009376-a35a92gh	274	9	lines	line	NNS
cord-009376-a35a92gh	274	10	,	,	,
cord-009376-a35a92gh	274	11	e.g.	e.g.	RB
cord-009376-a35a92gh	274	12	Hybridoma	Hybridoma	NNP
cord-009376-a35a92gh	274	13	and	and	CC
cord-009376-a35a92gh	274	14	CHO	CHO	NNP
cord-009376-a35a92gh	274	15	,	,	,
cord-009376-a35a92gh	274	16	are	be	VBP
cord-009376-a35a92gh	274	17	required	require	VBN
cord-009376-a35a92gh	274	18	to	to	TO
cord-009376-a35a92gh	274	19	perform	perform	VB
cord-009376-a35a92gh	274	20	Murine	Murine	NNP
cord-009376-a35a92gh	274	21	leukemia	leukemia	NN
cord-009376-a35a92gh	274	22	virus	virus	NN
cord-009376-a35a92gh	274	23	clearance	clearance	NN
cord-009376-a35a92gh	274	24	studies	study	NNS
cord-009376-a35a92gh	274	25	for	for	IN
cord-009376-a35a92gh	274	26	Phase	Phase	NNP
cord-009376-a35a92gh	274	27	IrII	IrII	NNP
cord-009376-a35a92gh	274	28	clinical	clinical	JJ
cord-009376-a35a92gh	274	29	trials	trial	NNS
cord-009376-a35a92gh	274	30	.	.	.
cord-009376-a35a92gh	275	1	Generally	generally	RB
cord-009376-a35a92gh	275	2	,	,	,
cord-009376-a35a92gh	275	3	processes	process	NNS
cord-009376-a35a92gh	275	4	for	for	IN
cord-009376-a35a92gh	275	5	phase	phase	NN
cord-009376-a35a92gh	275	6	III	iii	CD
cord-009376-a35a92gh	275	7	studies	study	NNS
cord-009376-a35a92gh	275	8	require	require	VBP
cord-009376-a35a92gh	275	9	clearance	clearance	NN
cord-009376-a35a92gh	275	10	of	of	IN
cord-009376-a35a92gh	275	11	three	three	CD
cord-009376-a35a92gh	275	12	or	or	CC
cord-009376-a35a92gh	275	13	more	more	JJR
cord-009376-a35a92gh	275	14	other	other	JJ
cord-009376-a35a92gh	275	15	Ž	ž	NN
cord-009376-a35a92gh	275	16	viruses	virus	NNS
cord-009376-a35a92gh	275	17	to	to	TO
cord-009376-a35a92gh	275	18	be	be	VB
cord-009376-a35a92gh	275	19	demonstrated	demonstrate	VBN
cord-009376-a35a92gh	275	20	Federal	Federal	NNP
cord-009376-a35a92gh	275	21	Register	Register	NNP
cord-009376-a35a92gh	275	22	,	,	,
cord-009376-a35a92gh	275	23	.	.	.
cord-009376-a35a92gh	276	1	1998	1998	CD
cord-009376-a35a92gh	276	2	.	.	.
cord-009376-a35a92gh	277	1	The	the	DT
cord-009376-a35a92gh	277	2	federal	federal	JJ
cord-009376-a35a92gh	277	3	guidelines	guideline	NNS
cord-009376-a35a92gh	277	4	specify	specify	VBP
cord-009376-a35a92gh	277	5	that	that	IN
cord-009376-a35a92gh	277	6	infectivity	infectivity	NN
cord-009376-a35a92gh	277	7	should	should	MD
cord-009376-a35a92gh	277	8	be	be	VB
cord-009376-a35a92gh	277	9	used	use	VBN
cord-009376-a35a92gh	277	10	,	,	,
cord-009376-a35a92gh	277	11	but	but	CC
cord-009376-a35a92gh	277	12	where	where	WRB
cord-009376-a35a92gh	277	13	this	this	DT
cord-009376-a35a92gh	277	14	is	be	VBZ
cord-009376-a35a92gh	277	15	not	not	RB
cord-009376-a35a92gh	277	16	technically	technically	RB
cord-009376-a35a92gh	277	17	feasible	feasible	JJ
cord-009376-a35a92gh	277	18	alternative	alternative	JJ
cord-009376-a35a92gh	277	19	technologies	technology	NNS
cord-009376-a35a92gh	277	20	may	may	MD
cord-009376-a35a92gh	277	21	be	be	VB
cord-009376-a35a92gh	277	22	justified	justify	VBN
cord-009376-a35a92gh	277	23	.	.	.
cord-009376-a35a92gh	278	1	However	however	RB
cord-009376-a35a92gh	278	2	,	,	,
cord-009376-a35a92gh	278	3	an	an	DT
cord-009376-a35a92gh	278	4	increasing	increase	VBG
cord-009376-a35a92gh	278	5	number	number	NN
cord-009376-a35a92gh	278	6	of	of	IN
cord-009376-a35a92gh	278	7	manufacturers	manufacturer	NNS
cord-009376-a35a92gh	278	8	are	be	VBP
cord-009376-a35a92gh	278	9	using	use	VBG
cord-009376-a35a92gh	278	10	both	both	CC
cord-009376-a35a92gh	278	11	infectivity	infectivity	NN
cord-009376-a35a92gh	278	12	and	and	CC
cord-009376-a35a92gh	278	13	Q	Q	NNP
cord-009376-a35a92gh	278	14	-	-	HYPH
cord-009376-a35a92gh	278	15	PCR	PCR	NNP
cord-009376-a35a92gh	278	16	to	to	TO
cord-009376-a35a92gh	278	17	demonstrate	demonstrate	VB
cord-009376-a35a92gh	278	18	viral	viral	JJ
cord-009376-a35a92gh	278	19	removal	removal	NN
cord-009376-a35a92gh	278	20	.	.	.
cord-009376-a35a92gh	279	1	Murine	murine	JJ
cord-009376-a35a92gh	279	2	leukemia	leukemia	NN
cord-009376-a35a92gh	279	3	viruses	virus	NNS
cord-009376-a35a92gh	279	4	are	be	VBP
cord-009376-a35a92gh	279	5	endogenous	endogenous	JJ
cord-009376-a35a92gh	279	6	proviruses	provirus	NNS
cord-009376-a35a92gh	279	7	that	that	WDT
cord-009376-a35a92gh	279	8	are	be	VBP
cord-009376-a35a92gh	279	9	divided	divide	VBN
cord-009376-a35a92gh	279	10	into	into	IN
cord-009376-a35a92gh	279	11	two	two	CD
cord-009376-a35a92gh	279	12	main	main	JJ
cord-009376-a35a92gh	279	13	groups	group	NNS
cord-009376-a35a92gh	279	14	,	,	,
cord-009376-a35a92gh	279	15	ecotropic	ecotropic	NNP
cord-009376-a35a92gh	279	16	and	and	CC
cord-009376-a35a92gh	279	17	non	non	JJ
cord-009376-a35a92gh	279	18	-	-	JJ
cord-009376-a35a92gh	279	19	ecotropic	ecotropic	JJ
cord-009376-a35a92gh	279	20	viruses	virus	NNS
cord-009376-a35a92gh	279	21	.	.	.
cord-009376-a35a92gh	280	1	Ecotropic	Ecotropic	NNS
cord-009376-a35a92gh	280	2	viruses	virus	NNS
cord-009376-a35a92gh	280	3	can	can	MD
cord-009376-a35a92gh	280	4	infect	infect	VB
cord-009376-a35a92gh	280	5	only	only	JJ
cord-009376-a35a92gh	280	6	mouse	mouse	NN
cord-009376-a35a92gh	280	7	cells	cell	NNS
cord-009376-a35a92gh	280	8	and	and	CC
cord-009376-a35a92gh	280	9	non	non	JJ
cord-009376-a35a92gh	280	10	-	-	JJ
cord-009376-a35a92gh	280	11	ecotropic	ecotropic	JJ
cord-009376-a35a92gh	280	12	viruses	virus	NNS
cord-009376-a35a92gh	280	13	are	be	VBP
cord-009376-a35a92gh	280	14	sub	sub	NN
cord-009376-a35a92gh	280	15	-	-	JJ
cord-009376-a35a92gh	280	16	divided	divided	JJ
cord-009376-a35a92gh	280	17	into	into	IN
cord-009376-a35a92gh	280	18	three	three	CD
cord-009376-a35a92gh	280	19	groups	group	NNS
cord-009376-a35a92gh	280	20	:	:	:
cord-009376-a35a92gh	280	21	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	280	22	;	;	:
cord-009376-a35a92gh	280	23	polytropic	polytropic	NNP
cord-009376-a35a92gh	280	24	;	;	:
cord-009376-a35a92gh	280	25	and	and	CC
cord-009376-a35a92gh	280	26	modified	modify	VBN
cord-009376-a35a92gh	280	27	polytropic	polytropic	NNP
cord-009376-a35a92gh	280	28	.	.	.
cord-009376-a35a92gh	281	1	Xenotropic	Xenotropic	NNP
cord-009376-a35a92gh	281	2	MLV	MLV	NNP
cord-009376-a35a92gh	281	3	infect	infect	VBP
cord-009376-a35a92gh	281	4	human	human	JJ
cord-009376-a35a92gh	281	5	cells	cell	NNS
cord-009376-a35a92gh	281	6	,	,	,
cord-009376-a35a92gh	281	7	but	but	CC
cord-009376-a35a92gh	281	8	not	not	RB
cord-009376-a35a92gh	281	9	their	-PRON-	PRP$
cord-009376-a35a92gh	281	10	natural	natural	JJ
cord-009376-a35a92gh	281	11	rodent	rodent	NN
cord-009376-a35a92gh	281	12	host	host	NN
cord-009376-a35a92gh	281	13	cells	cell	NNS
cord-009376-a35a92gh	281	14	.	.	.
cord-009376-a35a92gh	282	1	Polytropic	Polytropic	NNP
cord-009376-a35a92gh	282	2	MLVs	MLVs	NNP
cord-009376-a35a92gh	282	3	,	,	,
cord-009376-a35a92gh	282	4	also	also	RB
cord-009376-a35a92gh	282	5	Ž	Ž	NNP
cord-009376-a35a92gh	282	6	.	.	.
cord-009376-a35a92gh	283	1	termed	term	VBN
cord-009376-a35a92gh	283	2	mink	mink	NN
cord-009376-a35a92gh	283	3	-	-	HYPH
cord-009376-a35a92gh	283	4	cell	cell	NN
cord-009376-a35a92gh	283	5	focus	focus	NN
cord-009376-a35a92gh	284	1	MCF	MCF	NNP
cord-009376-a35a92gh	284	2	forming	form	VBG
cord-009376-a35a92gh	284	3	MLV	MLV	NNP
cord-009376-a35a92gh	284	4	can	can	MD
cord-009376-a35a92gh	284	5	infect	infect	VB
cord-009376-a35a92gh	284	6	both	both	DT
cord-009376-a35a92gh	284	7	rodent	rodent	NN
cord-009376-a35a92gh	284	8	and	and	CC
cord-009376-a35a92gh	284	9	non	non	JJ
cord-009376-a35a92gh	284	10	-	-	JJ
cord-009376-a35a92gh	284	11	rodent	rodent	JJ
cord-009376-a35a92gh	284	12	cells	cell	NNS
cord-009376-a35a92gh	284	13	,	,	,
cord-009376-a35a92gh	284	14	and	and	CC
cord-009376-a35a92gh	284	15	have	have	VBP
cord-009376-a35a92gh	284	16	a	a	DT
cord-009376-a35a92gh	284	17	broader	broad	JJR
cord-009376-a35a92gh	284	18	host	host	NN
cord-009376-a35a92gh	284	19	range	range	NN
cord-009376-a35a92gh	284	20	than	than	IN
cord-009376-a35a92gh	284	21	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	284	22	MLV	MLV	NNP
cord-009376-a35a92gh	284	23	.	.	.
cord-009376-a35a92gh	285	1	Unlike	unlike	IN
cord-009376-a35a92gh	285	2	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	285	3	and	and	CC
cord-009376-a35a92gh	285	4	ecotropic	ecotropic	JJ
cord-009376-a35a92gh	285	5	MLV	MLV	NNP
cord-009376-a35a92gh	285	6	,	,	,
cord-009376-a35a92gh	285	7	infectious	infectious	JJ
cord-009376-a35a92gh	285	8	polytropic	polytropic	NNS
cord-009376-a35a92gh	285	9	MLV	MLV	NNP
cord-009376-a35a92gh	285	10	do	do	VBP
cord-009376-a35a92gh	285	11	not	not	RB
cord-009376-a35a92gh	285	12	pre	pre	VB
cord-009376-a35a92gh	285	13	-	-	VB
cord-009376-a35a92gh	285	14	exist	exist	VB
cord-009376-a35a92gh	285	15	in	in	IN
cord-009376-a35a92gh	285	16	the	the	DT
cord-009376-a35a92gh	285	17	mouse	mouse	NN
cord-009376-a35a92gh	285	18	germ	germ	NN
cord-009376-a35a92gh	285	19	line	line	NN
cord-009376-a35a92gh	285	20	,	,	,
cord-009376-a35a92gh	285	21	but	but	CC
cord-009376-a35a92gh	285	22	can	can	MD
cord-009376-a35a92gh	285	23	arise	arise	VB
cord-009376-a35a92gh	285	24	by	by	IN
cord-009376-a35a92gh	285	25	recombination	recombination	NN
cord-009376-a35a92gh	285	26	between	between	IN
cord-009376-a35a92gh	285	27	infectious	infectious	JJ
cord-009376-a35a92gh	285	28	ecotropic	ecotropic	JJ
cord-009376-a35a92gh	285	29	MLV	MLV	NNP
cord-009376-a35a92gh	285	30	and	and	CC
cord-009376-a35a92gh	285	31	endogenous	endogenous	JJ
cord-009376-a35a92gh	285	32	MLV	MLV	NNP
cord-009376-a35a92gh	285	33	-	-	HYPH
cord-009376-a35a92gh	285	34	related	relate	VBN
cord-009376-a35a92gh	285	35	polytropic	polytropic	NNP
cord-009376-a35a92gh	285	36	sequences	sequence	NNS
cord-009376-a35a92gh	285	37	.	.	.
cord-009376-a35a92gh	286	1	In	in	IN
cord-009376-a35a92gh	286	2	certain	certain	JJ
cord-009376-a35a92gh	286	3	laboratory	laboratory	NN
cord-009376-a35a92gh	286	4	mouse	mouse	NN
cord-009376-a35a92gh	286	5	strains	strain	NNS
cord-009376-a35a92gh	286	6	,	,	,
cord-009376-a35a92gh	286	7	the	the	DT
cord-009376-a35a92gh	286	8	progeny	progeny	NN
cord-009376-a35a92gh	286	9	of	of	IN
cord-009376-a35a92gh	286	10	several	several	JJ
cord-009376-a35a92gh	286	11	non	non	JJ
cord-009376-a35a92gh	286	12	-	-	JJ
cord-009376-a35a92gh	286	13	ecotropic	ecotropic	JJ
cord-009376-a35a92gh	286	14	MLV	MLV	NNP
cord-009376-a35a92gh	286	15	can	can	MD
cord-009376-a35a92gh	286	16	recombine	recombine	VB
cord-009376-a35a92gh	286	17	with	with	IN
cord-009376-a35a92gh	286	18	exogenous	exogenous	JJ
cord-009376-a35a92gh	286	19	or	or	CC
cord-009376-a35a92gh	286	20	endogenous	endogenous	JJ
cord-009376-a35a92gh	286	21	MLV	MLV	NNP
cord-009376-a35a92gh	286	22	sequences	sequence	NNS
cord-009376-a35a92gh	286	23	to	to	TO
cord-009376-a35a92gh	286	24	result	result	VB
cord-009376-a35a92gh	286	25	in	in	IN
cord-009376-a35a92gh	286	26	oncogenic	oncogenic	JJ
cord-009376-a35a92gh	286	27	variants	variant	NNS
cord-009376-a35a92gh	286	28	such	such	JJ
cord-009376-a35a92gh	286	29	as	as	IN
cord-009376-a35a92gh	286	30	MCF	MCF	NNP
cord-009376-a35a92gh	286	31	virus	virus	NN
cord-009376-a35a92gh	286	32	Ž	ž	NN
cord-009376-a35a92gh	286	33	.	.	.
cord-009376-a35a92gh	287	1	Tomonaga	Tomonaga	NNP
cord-009376-a35a92gh	287	2	and	and	CC
cord-009376-a35a92gh	287	3	Coffin	Coffin	NNP
cord-009376-a35a92gh	287	4	,	,	,
cord-009376-a35a92gh	287	5	1998	1998	CD
cord-009376-a35a92gh	287	6	.	.	.
cord-009376-a35a92gh	288	1	Proviral	Proviral	NNP
cord-009376-a35a92gh	288	2	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	288	3	MLV	MLV	NNP
cord-009376-a35a92gh	288	4	that	that	WDT
cord-009376-a35a92gh	288	5	are	be	VBP
cord-009376-a35a92gh	288	6	capable	capable	JJ
cord-009376-a35a92gh	288	7	of	of	IN
cord-009376-a35a92gh	288	8	producing	produce	VBG
cord-009376-a35a92gh	288	9	infectious	infectious	JJ
cord-009376-a35a92gh	288	10	virus	virus	NN
cord-009376-a35a92gh	288	11	are	be	VBP
cord-009376-a35a92gh	288	12	present	present	JJ
cord-009376-a35a92gh	288	13	in	in	IN
cord-009376-a35a92gh	288	14	inbred	inbred	JJ
cord-009376-a35a92gh	288	15	mice	mice	NN
cord-009376-a35a92gh	288	16	populations	population	NNS
cord-009376-a35a92gh	288	17	,	,	,
cord-009376-a35a92gh	288	18	such	such	JJ
cord-009376-a35a92gh	288	19	as	as	IN
cord-009376-a35a92gh	288	20	Balb	Balb	NNP
cord-009376-a35a92gh	288	21	-	-	HYPH
cord-009376-a35a92gh	288	22	C.	C.	NNP
cord-009376-a35a92gh	288	23	An	an	DT
cord-009376-a35a92gh	288	24	endogenous	endogenous	JJ
cord-009376-a35a92gh	288	25	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	288	26	MLV	MLV	NNP
cord-009376-a35a92gh	288	27	,	,	,
cord-009376-a35a92gh	289	1	termed	term	VBN
cord-009376-a35a92gh	289	2	bx¨-1	bx¨-1	NNP
cord-009376-a35a92gh	289	3	appears	appear	VBZ
cord-009376-a35a92gh	289	4	to	to	TO
cord-009376-a35a92gh	289	5	be	be	VB
cord-009376-a35a92gh	289	6	the	the	DT
cord-009376-a35a92gh	289	7	LTR	LTR	NNP
cord-009376-a35a92gh	289	8	donor	donor	NN
cord-009376-a35a92gh	289	9	for	for	IN
cord-009376-a35a92gh	289	10	in	in	IN
cord-009376-a35a92gh	289	11	leukemogenic	leukemogenic	JJ
cord-009376-a35a92gh	289	12	MCF	MCF	NNP
cord-009376-a35a92gh	289	13	forming	form	VBG
cord-009376-a35a92gh	289	14	MLV	MLV	NNP
cord-009376-a35a92gh	289	15	.	.	.
cord-009376-a35a92gh	290	1	In	in	IN
cord-009376-a35a92gh	290	2	addition	addition	NN
cord-009376-a35a92gh	290	3	,	,	,
cord-009376-a35a92gh	290	4	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	290	5	MLV	MLV	NNP
cord-009376-a35a92gh	290	6	derived	derive	VBD
cord-009376-a35a92gh	290	7	from	from	IN
cord-009376-a35a92gh	290	8	the	the	DT
cord-009376-a35a92gh	290	9	Bx¨-1	Bx¨-1	NNP
cord-009376-a35a92gh	290	10	locus	locus	NN
cord-009376-a35a92gh	290	11	,	,	,
cord-009376-a35a92gh	290	12	first	first	RB
cord-009376-a35a92gh	290	13	described	describe	VBN
cord-009376-a35a92gh	290	14	in	in	IN
cord-009376-a35a92gh	290	15	BALBrc	BALBrc	NNP
cord-009376-a35a92gh	290	16	mice	mouse	NNS
cord-009376-a35a92gh	290	17	are	be	VBP
cord-009376-a35a92gh	290	18	infectious	infectious	JJ
cord-009376-a35a92gh	290	19	Ž	ž	NN
cord-009376-a35a92gh	290	20	.	.	.
cord-009376-a35a92gh	291	1	Kozak	Kozak	NNP
cord-009376-a35a92gh	291	2	and	and	CC
cord-009376-a35a92gh	291	3	Ruscetti	Ruscetti	NNP
cord-009376-a35a92gh	291	4	,	,	,
cord-009376-a35a92gh	291	5	1992	1992	CD
cord-009376-a35a92gh	291	6	.	.	.
cord-009376-a35a92gh	292	1	Murine	murine	JJ
cord-009376-a35a92gh	292	2	hybridoma	hybridoma	NN
cord-009376-a35a92gh	292	3	cells	cell	NNS
cord-009376-a35a92gh	292	4	used	use	VBN
cord-009376-a35a92gh	292	5	for	for	IN
cord-009376-a35a92gh	292	6	pharmaceutical	pharmaceutical	JJ
cord-009376-a35a92gh	292	7	monocolonal	monocolonal	NNP
cord-009376-a35a92gh	292	8	antibody	antibody	NNP
cord-009376-a35a92gh	292	9	production	production	NNP
cord-009376-a35a92gh	292	10	express	express	NNP
cord-009376-a35a92gh	292	11	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	292	12	MLV	MLV	NNP
cord-009376-a35a92gh	292	13	,	,	,
cord-009376-a35a92gh	292	14	and	and	CC
cord-009376-a35a92gh	292	15	regulatory	regulatory	JJ
cord-009376-a35a92gh	292	16	agencies	agency	NNS
cord-009376-a35a92gh	292	17	recommend	recommend	VBP
cord-009376-a35a92gh	292	18	that	that	IN
cord-009376-a35a92gh	292	19	retrovirus	retrovirus	NN
cord-009376-a35a92gh	292	20	should	should	MD
cord-009376-a35a92gh	292	21	be	be	VB
cord-009376-a35a92gh	292	22	inactivated	inactivate	VBN
cord-009376-a35a92gh	292	23	or	or	CC
cord-009376-a35a92gh	292	24	removed	remove	VBN
cord-009376-a35a92gh	292	25	during	during	IN
cord-009376-a35a92gh	292	26	manufacturing	manufacturing	NN
cord-009376-a35a92gh	292	27	.	.	.
cord-009376-a35a92gh	293	1	In	in	IN
cord-009376-a35a92gh	293	2	addition	addition	NN
cord-009376-a35a92gh	293	3	,	,	,
cord-009376-a35a92gh	293	4	MLV	MLV	NNP
cord-009376-a35a92gh	293	5	is	be	VBZ
cord-009376-a35a92gh	293	6	used	use	VBN
cord-009376-a35a92gh	293	7	as	as	IN
cord-009376-a35a92gh	293	8	a	a	DT
cord-009376-a35a92gh	293	9	model	model	NN
cord-009376-a35a92gh	293	10	virus	virus	NN
cord-009376-a35a92gh	293	11	to	to	TO
cord-009376-a35a92gh	293	12	evaluate	evaluate	VB
cord-009376-a35a92gh	293	13	the	the	DT
cord-009376-a35a92gh	293	14	elimination	elimination	NN
cord-009376-a35a92gh	293	15	of	of	IN
cord-009376-a35a92gh	293	16	non	non	JJ
cord-009376-a35a92gh	293	17	-	-	JJ
cord-009376-a35a92gh	293	18	infectious	infectious	JJ
cord-009376-a35a92gh	293	19	CHO	CHO	NNP
cord-009376-a35a92gh	293	20	cell	cell	NN
cord-009376-a35a92gh	293	21	retrovirus	retrovirus	NN
cord-009376-a35a92gh	293	22	particles	particle	NNS
cord-009376-a35a92gh	293	23	.	.	.
cord-009376-a35a92gh	294	1	Such	such	JJ
cord-009376-a35a92gh	294	2	retroviral	retroviral	JJ
cord-009376-a35a92gh	294	3	clearance	clearance	NN
cord-009376-a35a92gh	294	4	studies	study	NNS
cord-009376-a35a92gh	294	5	can	can	MD
cord-009376-a35a92gh	294	6	use	use	VB
cord-009376-a35a92gh	294	7	(	(	-LRB-
cord-009376-a35a92gh	294	8	)	)	-RRB-
cord-009376-a35a92gh	294	9	virus	virus	NN
cord-009376-a35a92gh	294	10	inactivation	inactivation	NN
cord-009376-a35a92gh	294	11	treatments	treatment	NNS
cord-009376-a35a92gh	294	12	such	such	JJ
cord-009376-a35a92gh	294	13	as	as	IN
cord-009376-a35a92gh	294	14	low	low	JJ
cord-009376-a35a92gh	294	15	pH	ph	NN
cord-009376-a35a92gh	294	16	of	of	IN
cord-009376-a35a92gh	294	17	elution	elution	NN
cord-009376-a35a92gh	294	18	buffers	buffer	NNS
cord-009376-a35a92gh	294	19	during	during	IN
cord-009376-a35a92gh	294	20	manufacturing	manufacturing	NN
cord-009376-a35a92gh	294	21	.	.	.
cord-009376-a35a92gh	295	1	Viral	viral	JJ
cord-009376-a35a92gh	295	2	clearance	clearance	NN
cord-009376-a35a92gh	295	3	studies	study	NNS
cord-009376-a35a92gh	295	4	in	in	IN
cord-009376-a35a92gh	295	5	scaled	scale	VBN
cord-009376-a35a92gh	295	6	down	down	RP
cord-009376-a35a92gh	295	7	conditions	condition	NNS
cord-009376-a35a92gh	295	8	,	,	,
cord-009376-a35a92gh	295	9	involve	involve	VBP
cord-009376-a35a92gh	295	10	spiking	spike	VBG
cord-009376-a35a92gh	295	11	the	the	DT
cord-009376-a35a92gh	295	12	load	load	NN
cord-009376-a35a92gh	295	13	sample	sample	NN
cord-009376-a35a92gh	295	14	with	with	IN
cord-009376-a35a92gh	295	15	a	a	DT
cord-009376-a35a92gh	295	16	known	know	VBN
cord-009376-a35a92gh	295	17	amount	amount	NN
cord-009376-a35a92gh	295	18	of	of	IN
cord-009376-a35a92gh	295	19	infectious	infectious	JJ
cord-009376-a35a92gh	295	20	virus	virus	NN
cord-009376-a35a92gh	295	21	to	to	TO
cord-009376-a35a92gh	295	22	allow	allow	VB
cord-009376-a35a92gh	295	23	assessment	assessment	NN
cord-009376-a35a92gh	295	24	of	of	IN
cord-009376-a35a92gh	295	25	retroviral	retroviral	JJ
cord-009376-a35a92gh	295	26	removal	removal	NN
cord-009376-a35a92gh	295	27	by	by	IN
cord-009376-a35a92gh	295	28	examination	examination	NN
cord-009376-a35a92gh	295	29	of	of	IN
cord-009376-a35a92gh	295	30	the	the	DT
cord-009376-a35a92gh	295	31	eluate	eluate	NN
cord-009376-a35a92gh	295	32	by	by	IN
cord-009376-a35a92gh	295	33	infectivity	infectivity	NN
cord-009376-a35a92gh	295	34	assay	assay	NN
cord-009376-a35a92gh	295	35	or	or	CC
cord-009376-a35a92gh	295	36	Q	q	NN
cord-009376-a35a92gh	295	37	-	-	HYPH
cord-009376-a35a92gh	295	38	PCR	PCR	NNP
cord-009376-a35a92gh	295	39	.	.	.
cord-009376-a35a92gh	296	1	However	however	RB
cord-009376-a35a92gh	296	2	,	,	,
cord-009376-a35a92gh	296	3	when	when	WRB
cord-009376-a35a92gh	296	4	buffers	buffer	NNS
cord-009376-a35a92gh	296	5	that	that	WDT
cord-009376-a35a92gh	296	6	inactivate	inactivate	VBP
cord-009376-a35a92gh	296	7	virus	virus	NN
cord-009376-a35a92gh	296	8	are	be	VBP
cord-009376-a35a92gh	296	9	used	use	VBN
cord-009376-a35a92gh	296	10	in	in	IN
cord-009376-a35a92gh	296	11	the	the	DT
cord-009376-a35a92gh	296	12	chromatography	chromatography	NN
cord-009376-a35a92gh	296	13	process	process	NN
cord-009376-a35a92gh	296	14	,	,	,
cord-009376-a35a92gh	296	15	the	the	DT
cord-009376-a35a92gh	296	16	reduction	reduction	NN
cord-009376-a35a92gh	296	17	of	of	IN
cord-009376-a35a92gh	296	18	infectious	infectious	JJ
cord-009376-a35a92gh	296	19	virus	virus	NN
cord-009376-a35a92gh	296	20	titre	titre	NN
cord-009376-a35a92gh	296	21	in	in	IN
cord-009376-a35a92gh	296	22	the	the	DT
cord-009376-a35a92gh	296	23	eluate	eluate	NN
cord-009376-a35a92gh	296	24	is	be	VBZ
cord-009376-a35a92gh	296	25	due	due	JJ
cord-009376-a35a92gh	296	26	to	to	IN
cord-009376-a35a92gh	296	27	the	the	DT
cord-009376-a35a92gh	296	28	combined	combined	JJ
cord-009376-a35a92gh	296	29	result	result	NN
cord-009376-a35a92gh	296	30	of	of	IN
cord-009376-a35a92gh	296	31	chro	chro	NN
cord-009376-a35a92gh	296	32	-	-	HYPH
cord-009376-a35a92gh	296	33	Ž	ž	NN
cord-009376-a35a92gh	296	34	matography	matography	NN
cord-009376-a35a92gh	296	35	removal	removal	NN
cord-009376-a35a92gh	296	36	and	and	CC
cord-009376-a35a92gh	296	37	buffer	buffer	NN
cord-009376-a35a92gh	296	38	inactivation	inactivation	NN
cord-009376-a35a92gh	296	39	Lau	Lau	NNP
cord-009376-a35a92gh	296	40	.	.	.
cord-009376-a35a92gh	297	1	et	et	NNP
cord-009376-a35a92gh	297	2	al	al	NNP
cord-009376-a35a92gh	297	3	.	.	NNP
cord-009376-a35a92gh	297	4	,	,	,
cord-009376-a35a92gh	297	5	1999	1999	CD
cord-009376-a35a92gh	297	6	.	.	.
cord-009376-a35a92gh	298	1	In	in	IN
cord-009376-a35a92gh	298	2	these	these	DT
cord-009376-a35a92gh	298	3	cases	case	NNS
cord-009376-a35a92gh	298	4	,	,	,
cord-009376-a35a92gh	298	5	infectivity	infectivity	NN
cord-009376-a35a92gh	298	6	assays	assay	NNS
cord-009376-a35a92gh	298	7	will	will	MD
cord-009376-a35a92gh	298	8	not	not	RB
cord-009376-a35a92gh	298	9	provide	provide	VB
cord-009376-a35a92gh	298	10	clearance	clearance	NN
cord-009376-a35a92gh	298	11	information	information	NN
cord-009376-a35a92gh	298	12	,	,	,
cord-009376-a35a92gh	298	13	whereas	whereas	IN
cord-009376-a35a92gh	298	14	Q	Q	NNP
cord-009376-a35a92gh	298	15	-	-	HYPH
cord-009376-a35a92gh	298	16	PCR	PCR	NNP
cord-009376-a35a92gh	298	17	resolves	resolve	VBZ
cord-009376-a35a92gh	298	18	this	this	DT
cord-009376-a35a92gh	298	19	problem	problem	NN
cord-009376-a35a92gh	298	20	.	.	.
cord-009376-a35a92gh	299	1	Initially	initially	RB
cord-009376-a35a92gh	299	2	,	,	,
cord-009376-a35a92gh	299	3	it	-PRON-	PRP
cord-009376-a35a92gh	299	4	is	be	VBZ
cord-009376-a35a92gh	299	5	essential	essential	JJ
cord-009376-a35a92gh	299	6	to	to	TO
cord-009376-a35a92gh	299	7	qualify	qualify	VB
cord-009376-a35a92gh	299	8	the	the	DT
cord-009376-a35a92gh	299	9	viral	viral	JJ
cord-009376-a35a92gh	299	10	clearance	clearance	NN
cord-009376-a35a92gh	299	11	Q	Q	NNP
cord-009376-a35a92gh	299	12	-	-	HYPH
cord-009376-a35a92gh	299	13	PCR	pcr	NN
cord-009376-a35a92gh	299	14	assay	assay	NN
cord-009376-a35a92gh	299	15	using	use	VBG
cord-009376-a35a92gh	299	16	quantitative	quantitative	JJ
cord-009376-a35a92gh	299	17	results	result	NNS
cord-009376-a35a92gh	299	18	on	on	IN
cord-009376-a35a92gh	299	19	both	both	CC
cord-009376-a35a92gh	299	20	the	the	DT
cord-009376-a35a92gh	299	21	spiked	spiked	JJ
cord-009376-a35a92gh	299	22	load	load	NN
cord-009376-a35a92gh	299	23	and	and	CC
cord-009376-a35a92gh	299	24	unspiked	unspiked	JJ
cord-009376-a35a92gh	299	25	load	load	NN
cord-009376-a35a92gh	299	26	control	control	NN
cord-009376-a35a92gh	299	27	.	.	.
cord-009376-a35a92gh	300	1	For	for	IN
cord-009376-a35a92gh	300	2	cell	cell	NN
cord-009376-a35a92gh	300	3	lines	line	NNS
cord-009376-a35a92gh	300	4	other	other	JJ
cord-009376-a35a92gh	300	5	than	than	IN
cord-009376-a35a92gh	300	6	murine	murine	JJ
cord-009376-a35a92gh	300	7	hybrodoma	hybrodoma	NNS
cord-009376-a35a92gh	300	8	,	,	,
cord-009376-a35a92gh	300	9	this	this	DT
cord-009376-a35a92gh	300	10	ensures	ensure	VBZ
cord-009376-a35a92gh	300	11	the	the	DT
cord-009376-a35a92gh	300	12	absence	absence	NN
cord-009376-a35a92gh	300	13	of	of	IN
cord-009376-a35a92gh	300	14	non	non	JJ
cord-009376-a35a92gh	300	15	-	-	JJ
cord-009376-a35a92gh	300	16	specific	specific	JJ
cord-009376-a35a92gh	300	17	interactions	interaction	NNS
cord-009376-a35a92gh	300	18	that	that	WDT
cord-009376-a35a92gh	300	19	obscure	obscure	VBP
cord-009376-a35a92gh	300	20	the	the	DT
cord-009376-a35a92gh	300	21	clearance	clearance	NN
cord-009376-a35a92gh	300	22	data	datum	NNS
cord-009376-a35a92gh	300	23	.	.	.
cord-009376-a35a92gh	301	1	For	for	IN
cord-009376-a35a92gh	301	2	retroviral	retroviral	JJ
cord-009376-a35a92gh	301	3	nucleic	nucleic	JJ
cord-009376-a35a92gh	301	4	acid	acid	NN
cord-009376-a35a92gh	301	5	clearance	clearance	NN
cord-009376-a35a92gh	301	6	,	,	,
cord-009376-a35a92gh	301	7	it	-PRON-	PRP
cord-009376-a35a92gh	301	8	is	be	VBZ
cord-009376-a35a92gh	301	9	essential	essential	JJ
cord-009376-a35a92gh	301	10	to	to	TO
cord-009376-a35a92gh	301	11	perform	perform	VB
cord-009376-a35a92gh	301	12	DNA	DNA	NNP
cord-009376-a35a92gh	301	13	and	and	CC
cord-009376-a35a92gh	301	14	RNA	RNA	NNP
cord-009376-a35a92gh	302	1	copy	copy	NN
cord-009376-a35a92gh	302	2	number	number	NN
cord-009376-a35a92gh	302	3	quantification	quantification	NN
cord-009376-a35a92gh	302	4	on	on	IN
cord-009376-a35a92gh	302	5	the	the	DT
cord-009376-a35a92gh	302	6	viral	viral	JJ
cord-009376-a35a92gh	302	7	spike	spike	NN
cord-009376-a35a92gh	302	8	and	and	CC
cord-009376-a35a92gh	302	9	any	any	DT
cord-009376-a35a92gh	302	10	spiked	spiked	JJ
cord-009376-a35a92gh	302	11	column	column	NN
cord-009376-a35a92gh	302	12	elutions	elution	NNS
cord-009376-a35a92gh	302	13	.	.	.
cord-009376-a35a92gh	303	1	Analysis	analysis	NN
cord-009376-a35a92gh	303	2	of	of	IN
cord-009376-a35a92gh	303	3	the	the	DT
cord-009376-a35a92gh	303	4	X	X	NNP
cord-009376-a35a92gh	303	5	-	-	NN
cord-009376-a35a92gh	303	6	MLV	MLV	NNP
cord-009376-a35a92gh	303	7	nucleic	nucleic	JJ
cord-009376-a35a92gh	303	8	acid	acid	NN
cord-009376-a35a92gh	303	9	copy	copy	NN
cord-009376-a35a92gh	303	10	number	number	NN
cord-009376-a35a92gh	303	11	in	in	IN
cord-009376-a35a92gh	303	12	the	the	DT
cord-009376-a35a92gh	303	13	absence	absence	NN
cord-009376-a35a92gh	303	14	of	of	IN
cord-009376-a35a92gh	303	15	RT	RT	NNP
cord-009376-a35a92gh	303	16	enzyme	enzyme	NN
cord-009376-a35a92gh	303	17	allows	allow	VBZ
cord-009376-a35a92gh	303	18	an	an	DT
cord-009376-a35a92gh	303	19	estimation	estimation	NN
cord-009376-a35a92gh	303	20	of	of	IN
cord-009376-a35a92gh	303	21	the	the	DT
cord-009376-a35a92gh	303	22	percentage	percentage	NN
cord-009376-a35a92gh	303	23	of	of	IN
cord-009376-a35a92gh	303	24	proviral	proviral	JJ
cord-009376-a35a92gh	303	25	DNA	dna	NN
cord-009376-a35a92gh	303	26	targets	target	NNS
cord-009376-a35a92gh	303	27	which	which	WDT
cord-009376-a35a92gh	303	28	can	can	MD
cord-009376-a35a92gh	303	29	result	result	VB
cord-009376-a35a92gh	303	30	from	from	IN
cord-009376-a35a92gh	303	31	the	the	DT
cord-009376-a35a92gh	303	32	background	background	NN
cord-009376-a35a92gh	303	33	residual	residual	JJ
cord-009376-a35a92gh	303	34	host	host	NN
cord-009376-a35a92gh	303	35	cell	cell	NN
cord-009376-a35a92gh	303	36	DNA	dna	NN
cord-009376-a35a92gh	303	37	of	of	IN
cord-009376-a35a92gh	303	38	the	the	DT
cord-009376-a35a92gh	303	39	viral	viral	JJ
cord-009376-a35a92gh	303	40	production	production	NN
cord-009376-a35a92gh	303	41	cell	cell	NN
cord-009376-a35a92gh	303	42	line	line	NN
cord-009376-a35a92gh	303	43	.	.	.
cord-009376-a35a92gh	304	1	By	by	IN
cord-009376-a35a92gh	304	2	recalculating	recalculate	VBG
cord-009376-a35a92gh	304	3	the	the	DT
cord-009376-a35a92gh	304	4	RNA	RNA	NNP
cord-009376-a35a92gh	304	5	copy	copy	NN
cord-009376-a35a92gh	304	6	number	number	NN
cord-009376-a35a92gh	304	7	,	,	,
cord-009376-a35a92gh	304	8	this	this	DT
cord-009376-a35a92gh	304	9	ensures	ensure	VBZ
cord-009376-a35a92gh	304	10	that	that	DT
cord-009376-a35a92gh	304	11	detection	detection	NN
cord-009376-a35a92gh	304	12	of	of	IN
cord-009376-a35a92gh	304	13	viral	viral	JJ
cord-009376-a35a92gh	304	14	RNA	RNA	NNP
cord-009376-a35a92gh	304	15	genome	genome	NN
cord-009376-a35a92gh	304	16	is	be	VBZ
cord-009376-a35a92gh	304	17	achieved	achieve	VBN
cord-009376-a35a92gh	304	18	and	and	CC
cord-009376-a35a92gh	304	19	the	the	DT
cord-009376-a35a92gh	304	20	results	result	NNS
cord-009376-a35a92gh	304	21	take	take	VBP
cord-009376-a35a92gh	304	22	into	into	IN
cord-009376-a35a92gh	304	23	account	account	NN
cord-009376-a35a92gh	304	24	any	any	DT
cord-009376-a35a92gh	304	25	background	background	NN
cord-009376-a35a92gh	304	26	integrated	integrate	VBN
cord-009376-a35a92gh	304	27	provirus	provirus	NNP
cord-009376-a35a92gh	304	28	present	present	NN
cord-009376-a35a92gh	304	29	in	in	IN
cord-009376-a35a92gh	304	30	residual	residual	JJ
cord-009376-a35a92gh	304	31	production	production	NN
cord-009376-a35a92gh	304	32	cell	cell	NN
cord-009376-a35a92gh	304	33	genomic	genomic	JJ
cord-009376-a35a92gh	304	34	DNA	dna	NN
cord-009376-a35a92gh	304	35	.	.	.
cord-009376-a35a92gh	305	1	The	the	DT
cord-009376-a35a92gh	305	2	difference	difference	NN
cord-009376-a35a92gh	305	3	between	between	IN
cord-009376-a35a92gh	305	4	two	two	CD
cord-009376-a35a92gh	305	5	RNA	RNA	NNP
cord-009376-a35a92gh	305	6	copy	copy	NN
cord-009376-a35a92gh	305	7	numbers	number	NNS
cord-009376-a35a92gh	305	8	obtained	obtain	VBN
cord-009376-a35a92gh	305	9	from	from	IN
cord-009376-a35a92gh	305	10	the	the	DT
cord-009376-a35a92gh	305	11	viral	viral	JJ
cord-009376-a35a92gh	305	12	spike	spike	NN
cord-009376-a35a92gh	305	13	and	and	CC
cord-009376-a35a92gh	305	14	spiked	spiked	JJ
cord-009376-a35a92gh	305	15	column	column	NN
cord-009376-a35a92gh	305	16	elutions	elution	NNS
cord-009376-a35a92gh	305	17	allows	allow	VBZ
cord-009376-a35a92gh	305	18	calculation	calculation	NN
cord-009376-a35a92gh	305	19	of	of	IN
cord-009376-a35a92gh	305	20	Ž	Ž	NNP
cord-009376-a35a92gh	305	21	.	.	.
cord-009376-a35a92gh	306	1	viral	viral	JJ
cord-009376-a35a92gh	306	2	removal	removal	NN
cord-009376-a35a92gh	307	1	Fig	Fig	NNP
cord-009376-a35a92gh	307	2	.	.	.
cord-009376-a35a92gh	308	1	5	5	LS
cord-009376-a35a92gh	308	2	.	.	.
cord-009376-a35a92gh	309	1	For	for	IN
cord-009376-a35a92gh	309	2	the	the	DT
cord-009376-a35a92gh	309	3	column	column	NN
cord-009376-a35a92gh	309	4	elution	elution	NN
cord-009376-a35a92gh	309	5	samples	sample	NNS
cord-009376-a35a92gh	309	6	that	that	WDT
cord-009376-a35a92gh	309	7	already	already	RB
cord-009376-a35a92gh	309	8	contain	contain	VBP
cord-009376-a35a92gh	309	9	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	309	10	MLV	MLV	NNP
cord-009376-a35a92gh	309	11	,	,	,
cord-009376-a35a92gh	309	12	during	during	IN
cord-009376-a35a92gh	309	13	RNA	RNA	NNP
cord-009376-a35a92gh	309	14	extraction	extraction	NN
cord-009376-a35a92gh	309	15	a	a	DT
cord-009376-a35a92gh	309	16	different	different	JJ
cord-009376-a35a92gh	309	17	virus	virus	NN
cord-009376-a35a92gh	309	18	can	can	MD
cord-009376-a35a92gh	309	19	be	be	VB
cord-009376-a35a92gh	309	20	spiked	spike	VBN
cord-009376-a35a92gh	309	21	as	as	IN
cord-009376-a35a92gh	309	22	an	an	DT
cord-009376-a35a92gh	309	23	internal	internal	JJ
cord-009376-a35a92gh	309	24	extractionramplification	extractionramplification	NN
cord-009376-a35a92gh	309	25	control	control	NN
cord-009376-a35a92gh	309	26	in	in	IN
cord-009376-a35a92gh	309	27	order	order	NN
cord-009376-a35a92gh	309	28	to	to	TO
cord-009376-a35a92gh	309	29	distinguish	distinguish	VB
cord-009376-a35a92gh	309	30	it	-PRON-	PRP
cord-009376-a35a92gh	309	31	from	from	IN
cord-009376-a35a92gh	309	32	the	the	DT
cord-009376-a35a92gh	309	33	MLV	MLV	NNP
cord-009376-a35a92gh	309	34	already	already	RB
cord-009376-a35a92gh	309	35	present	present	JJ
cord-009376-a35a92gh	309	36	.	.	.
cord-009376-a35a92gh	310	1	The	the	DT
cord-009376-a35a92gh	310	2	internal	internal	JJ
cord-009376-a35a92gh	310	3	control	control	NN
cord-009376-a35a92gh	310	4	spike	spike	NN
cord-009376-a35a92gh	310	5	should	should	MD
cord-009376-a35a92gh	310	6	not	not	RB
cord-009376-a35a92gh	310	7	cross	cross	VB
cord-009376-a35a92gh	310	8	-	-	JJ
cord-009376-a35a92gh	310	9	react	react	VB
cord-009376-a35a92gh	310	10	with	with	IN
cord-009376-a35a92gh	310	11	the	the	DT
cord-009376-a35a92gh	310	12	actual	actual	JJ
cord-009376-a35a92gh	310	13	xenotropic	xenotropic	NNP
cord-009376-a35a92gh	310	14	MLV	MLV	NNP
cord-009376-a35a92gh	310	15	to	to	TO
cord-009376-a35a92gh	310	16	simplify	simplify	VB
cord-009376-a35a92gh	310	17	calculations	calculation	NNS
cord-009376-a35a92gh	310	18	.	.	.
cord-009376-a35a92gh	311	1	An	an	DT
cord-009376-a35a92gh	311	2	unrelated	unrelated	JJ
cord-009376-a35a92gh	311	3	RNA	RNA	NNP
cord-009376-a35a92gh	311	4	virus	virus	NN
cord-009376-a35a92gh	311	5	of	of	IN
cord-009376-a35a92gh	311	6	known	know	VBN
cord-009376-a35a92gh	311	7	TCID	TCID	NNP
cord-009376-a35a92gh	311	8	50	50	CD
cord-009376-a35a92gh	311	9	or	or	CC
cord-009376-a35a92gh	311	10	naked	naked	JJ
cord-009376-a35a92gh	311	11	viral	viral	JJ
cord-009376-a35a92gh	311	12	RNA	RNA	NNP
cord-009376-a35a92gh	311	13	can	can	MD
cord-009376-a35a92gh	311	14	be	be	VB
cord-009376-a35a92gh	311	15	used	use	VBN
cord-009376-a35a92gh	311	16	to	to	TO
cord-009376-a35a92gh	311	17	control	control	VB
cord-009376-a35a92gh	311	18	for	for	IN
cord-009376-a35a92gh	311	19	PCRrextraction	pcrrextraction	NN
cord-009376-a35a92gh	311	20	interference	interference	NN
cord-009376-a35a92gh	311	21	and	and	CC
cord-009376-a35a92gh	311	22	viral	viral	JJ
cord-009376-a35a92gh	311	23	recovery	recovery	NN
cord-009376-a35a92gh	311	24	during	during	IN
cord-009376-a35a92gh	311	25	extraction	extraction	NN
cord-009376-a35a92gh	311	26	.	.	.
cord-009376-a35a92gh	312	1	Viral	viral	JJ
cord-009376-a35a92gh	312	2	removal	removal	NN
cord-009376-a35a92gh	312	3	can	can	MD
cord-009376-a35a92gh	312	4	be	be	VB
cord-009376-a35a92gh	312	5	obtained	obtain	VBN
cord-009376-a35a92gh	312	6	by	by	IN
cord-009376-a35a92gh	312	7	calculating	calculate	VBG
cord-009376-a35a92gh	312	8	the	the	DT
cord-009376-a35a92gh	312	9	mean	mean	NNP
cord-009376-a35a92gh	312	10	RNA	RNA	NNP
cord-009376-a35a92gh	312	11	copy	copy	NN
cord-009376-a35a92gh	312	12	number	number	NN
cord-009376-a35a92gh	312	13	of	of	IN
cord-009376-a35a92gh	312	14	the	the	DT
cord-009376-a35a92gh	312	15	load	load	NN
cord-009376-a35a92gh	312	16	,	,	,
cord-009376-a35a92gh	312	17	hold	hold	VB
cord-009376-a35a92gh	312	18	control	control	NN
cord-009376-a35a92gh	312	19	and	and	CC
cord-009376-a35a92gh	312	20	eluate	eluate	NN
cord-009376-a35a92gh	312	21	from	from	IN
cord-009376-a35a92gh	312	22	column	column	NN
cord-009376-a35a92gh	312	23	runs	run	NNS
cord-009376-a35a92gh	312	24	.	.	.
cord-009376-a35a92gh	313	1	In	in	IN
cord-009376-a35a92gh	313	2	addition	addition	NN
cord-009376-a35a92gh	313	3	to	to	IN
cord-009376-a35a92gh	313	4	X	x	NN
cord-009376-a35a92gh	313	5	-	-	JJ
cord-009376-a35a92gh	313	6	MLV	MLV	NNP
cord-009376-a35a92gh	313	7	quantification	quantification	NN
cord-009376-a35a92gh	313	8	,	,	,
cord-009376-a35a92gh	313	9	Q	Q	NNP
cord-009376-a35a92gh	313	10	-	-	HYPH
cord-009376-a35a92gh	313	11	PCR	PCR	NNP
cord-009376-a35a92gh	313	12	assays	assay	NNS
cord-009376-a35a92gh	313	13	to	to	TO
cord-009376-a35a92gh	313	14	measure	measure	VB
cord-009376-a35a92gh	313	15	clearance	clearance	NN
cord-009376-a35a92gh	313	16	of	of	IN
cord-009376-a35a92gh	313	17	the	the	DT
cord-009376-a35a92gh	313	18	endogenous	endogenous	JJ
cord-009376-a35a92gh	313	19	Ž	ž	NN
cord-009376-a35a92gh	313	20	.	.	.
cord-009376-a35a92gh	314	1	retrovirus	retrovirus	NN
cord-009376-a35a92gh	314	2	from	from	IN
cord-009376-a35a92gh	314	3	Chinese	chinese	JJ
cord-009376-a35a92gh	314	4	hamster	hamster	NN
cord-009376-a35a92gh	314	5	ovary	ovary	NN
cord-009376-a35a92gh	314	6	CHO	CHO	NNP
cord-009376-a35a92gh	314	7	Ž	Ž	NNP
cord-009376-a35a92gh	314	8	cells	cell	NNS
cord-009376-a35a92gh	314	9	and	and	CC
cord-009376-a35a92gh	314	10	SV40	SV40	NNP
cord-009376-a35a92gh	314	11	are	be	VBP
cord-009376-a35a92gh	314	12	now	now	RB
cord-009376-a35a92gh	314	13	available	available	JJ
cord-009376-a35a92gh	315	1	Shi	Shi	NNP
cord-009376-a35a92gh	315	2	et	et	FW
cord-009376-a35a92gh	315	3	al	al	NNP
cord-009376-a35a92gh	315	4	.	.	.
cord-009376-a35a92gh	316	1	,	,	,
cord-009376-a35a92gh	316	2	.	.	.
cord-009376-a35a92gh	317	1	1999a	1999a	CD
cord-009376-a35a92gh	317	2	,	,	,
cord-009376-a35a92gh	317	3	b	b	NN
cord-009376-a35a92gh	317	4	;	;	:
cord-009376-a35a92gh	317	5	de	de	NNP
cord-009376-a35a92gh	317	6	Wit	Wit	NNP
cord-009376-a35a92gh	317	7	et	et	NNP
cord-009376-a35a92gh	317	8	al	al	NNP
cord-009376-a35a92gh	317	9	.	.	NNP
cord-009376-a35a92gh	317	10	,	,	,
cord-009376-a35a92gh	317	11	2000	2000	CD
cord-009376-a35a92gh	317	12	.	.	.
cord-009376-a35a92gh	318	1	Taken	take	VBN
cord-009376-a35a92gh	318	2	together	together	RB
cord-009376-a35a92gh	318	3	these	these	DT
cord-009376-a35a92gh	318	4	approaches	approach	NNS
cord-009376-a35a92gh	318	5	will	will	MD
cord-009376-a35a92gh	318	6	allow	allow	VB
cord-009376-a35a92gh	318	7	the	the	DT
cord-009376-a35a92gh	318	8	manufacturer	manufacturer	NN
cord-009376-a35a92gh	318	9	's	's	POS
cord-009376-a35a92gh	318	10	clearance	clearance	NN
cord-009376-a35a92gh	318	11	process	process	NN
cord-009376-a35a92gh	318	12	to	to	TO
cord-009376-a35a92gh	318	13	be	be	VB
cord-009376-a35a92gh	318	14	assessed	assess	VBN
cord-009376-a35a92gh	318	15	for	for	IN
cord-009376-a35a92gh	318	16	the	the	DT
cord-009376-a35a92gh	318	17	ability	ability	NN
cord-009376-a35a92gh	318	18	to	to	TO
cord-009376-a35a92gh	318	19	remove	remove	VB
cord-009376-a35a92gh	318	20	logs	log	NNS
cord-009376-a35a92gh	318	21	of	of	IN
cord-009376-a35a92gh	318	22	viral	viral	JJ
cord-009376-a35a92gh	318	23	genomes	genome	NNS
cord-009376-a35a92gh	318	24	and	and	CC
cord-009376-a35a92gh	318	25	thereby	thereby	RB
cord-009376-a35a92gh	318	26	pave	pave	VB
cord-009376-a35a92gh	318	27	the	the	DT
cord-009376-a35a92gh	318	28	way	way	NN
cord-009376-a35a92gh	318	29	for	for	IN
cord-009376-a35a92gh	318	30	optimisation	optimisation	NN
cord-009376-a35a92gh	318	31	of	of	IN
cord-009376-a35a92gh	318	32	procedures	procedure	NNS
cord-009376-a35a92gh	318	33	to	to	TO
cord-009376-a35a92gh	318	34	generate	generate	VB
cord-009376-a35a92gh	318	35	maximum	maximum	JJ
cord-009376-a35a92gh	318	36	viral	viral	JJ
cord-009376-a35a92gh	318	37	clearance	clearance	NN
cord-009376-a35a92gh	318	38	.	.	.
cord-009376-a35a92gh	319	1	Manufacturers	manufacturer	NNS
cord-009376-a35a92gh	319	2	of	of	IN
cord-009376-a35a92gh	319	3	biopharmaceuticals	biopharmaceutical	NNS
cord-009376-a35a92gh	319	4	must	must	MD
cord-009376-a35a92gh	319	5	ensure	ensure	VB
cord-009376-a35a92gh	319	6	that	that	IN
cord-009376-a35a92gh	319	7	final	final	JJ
cord-009376-a35a92gh	319	8	products	product	NNS
cord-009376-a35a92gh	319	9	produced	produce	VBN
cord-009376-a35a92gh	319	10	from	from	IN
cord-009376-a35a92gh	319	11	starting	start	VBG
cord-009376-a35a92gh	319	12	cell	cell	NN
cord-009376-a35a92gh	319	13	substrates	substrate	NNS
cord-009376-a35a92gh	319	14	contain	contain	VBP
cord-009376-a35a92gh	319	15	minimum	minimum	JJ
cord-009376-a35a92gh	319	16	levels	level	NNS
cord-009376-a35a92gh	319	17	of	of	IN
cord-009376-a35a92gh	319	18	residual	residual	JJ
cord-009376-a35a92gh	319	19	host	host	NN
cord-009376-a35a92gh	319	20	cell	cell	NN
cord-009376-a35a92gh	319	21	DNA	dna	NN
cord-009376-a35a92gh	319	22	.	.	.
cord-009376-a35a92gh	320	1	Current	current	JJ
cord-009376-a35a92gh	320	2	regulatory	regulatory	JJ
cord-009376-a35a92gh	320	3	guidelines	guideline	NNS
cord-009376-a35a92gh	320	4	established	establish	VBN
cord-009376-a35a92gh	320	5	by	by	IN
cord-009376-a35a92gh	320	6	the	the	DT
cord-009376-a35a92gh	320	7	FDA	FDA	NNP
cord-009376-a35a92gh	320	8	and	and	CC
cord-009376-a35a92gh	320	9	WHO	who	WP
cord-009376-a35a92gh	320	10	recommend	recommend	VBP
cord-009376-a35a92gh	320	11	that	that	IN
cord-009376-a35a92gh	320	12	products	product	NNS
cord-009376-a35a92gh	320	13	manufactured	manufacture	VBN
cord-009376-a35a92gh	320	14	from	from	IN
cord-009376-a35a92gh	320	15	continuous	continuous	JJ
cord-009376-a35a92gh	320	16	cell	cell	NN
cord-009376-a35a92gh	320	17	lines	line	NNS
cord-009376-a35a92gh	320	18	contain	contain	VBP
cord-009376-a35a92gh	320	19	less	less	JJR
cord-009376-a35a92gh	320	20	than	than	IN
cord-009376-a35a92gh	320	21	100	100	CD
cord-009376-a35a92gh	320	22	pg	pg	NNS
cord-009376-a35a92gh	320	23	of	of	IN
cord-009376-a35a92gh	320	24	cellular	cellular	JJ
cord-009376-a35a92gh	320	25	DNA	dna	NN
cord-009376-a35a92gh	320	26	per	per	IN
cord-009376-a35a92gh	320	27	dose	dose	NN
cord-009376-a35a92gh	320	28	Ž	ž	NN
cord-009376-a35a92gh	320	29	.	.	.
cord-009376-a35a92gh	321	1	Lewis	Lewis	NNP
cord-009376-a35a92gh	321	2	et	et	NNP
cord-009376-a35a92gh	321	3	al	al	NNP
cord-009376-a35a92gh	321	4	.	.	NNP
cord-009376-a35a92gh	321	5	,	,	,
cord-009376-a35a92gh	321	6	1999a	1999a	CD
cord-009376-a35a92gh	321	7	.	.	.
cord-009376-a35a92gh	322	1	The	the	DT
cord-009376-a35a92gh	322	2	dangers	danger	NNS
cord-009376-a35a92gh	322	3	of	of	IN
cord-009376-a35a92gh	322	4	residual	residual	JJ
cord-009376-a35a92gh	322	5	DNA	dna	NN
cord-009376-a35a92gh	322	6	include	include	VBP
cord-009376-a35a92gh	322	7	:	:	:
cord-009376-a35a92gh	322	8	malignant	malignant	JJ
cord-009376-a35a92gh	322	9	transformation	transformation	NN
cord-009376-a35a92gh	322	10	by	by	IN
cord-009376-a35a92gh	322	11	activated	activate	VBN
cord-009376-a35a92gh	322	12	cellular	cellular	JJ
cord-009376-a35a92gh	322	13	andror	andror	VBN
cord-009376-a35a92gh	322	14	viral	viral	JJ
cord-009376-a35a92gh	322	15	oncogenes	oncogene	NNS
cord-009376-a35a92gh	322	16	;	;	,
cord-009376-a35a92gh	322	17	aberrant	aberrant	JJ
cord-009376-a35a92gh	322	18	gene	gene	NN
cord-009376-a35a92gh	322	19	expression	expression	NN
cord-009376-a35a92gh	322	20	by	by	IN
cord-009376-a35a92gh	322	21	insertion	insertion	NN
cord-009376-a35a92gh	322	22	of	of	IN
cord-009376-a35a92gh	322	23	sequences	sequence	NNS
cord-009376-a35a92gh	322	24	in	in	IN
cord-009376-a35a92gh	322	25	sensitive	sensitive	JJ
cord-009376-a35a92gh	322	26	control	control	NN
cord-009376-a35a92gh	322	27	regions	region	NNS
cord-009376-a35a92gh	322	28	of	of	IN
cord-009376-a35a92gh	322	29	genes	gene	NNS
cord-009376-a35a92gh	322	30	;	;	:
cord-009376-a35a92gh	322	31	as	as	RB
cord-009376-a35a92gh	322	32	well	well	RB
cord-009376-a35a92gh	322	33	as	as	IN
cord-009376-a35a92gh	322	34	the	the	DT
cord-009376-a35a92gh	322	35	production	production	NN
cord-009376-a35a92gh	322	36	of	of	IN
cord-009376-a35a92gh	322	37	infectious	infectious	JJ
cord-009376-a35a92gh	322	38	viruses	virus	NNS
cord-009376-a35a92gh	322	39	from	from	IN
cord-009376-a35a92gh	322	40	viral	viral	JJ
cord-009376-a35a92gh	322	41	DNA	dna	NN
cord-009376-a35a92gh	322	42	.	.	.
cord-009376-a35a92gh	323	1	In	in	IN
cord-009376-a35a92gh	323	2	response	response	NN
cord-009376-a35a92gh	323	3	to	to	IN
cord-009376-a35a92gh	323	4	these	these	DT
cord-009376-a35a92gh	323	5	concerns	concern	NNS
cord-009376-a35a92gh	323	6	,	,	,
cord-009376-a35a92gh	323	7	a	a	DT
cord-009376-a35a92gh	323	8	number	number	NN
cord-009376-a35a92gh	323	9	of	of	IN
cord-009376-a35a92gh	323	10	Q	Q	NNP
cord-009376-a35a92gh	323	11	-	-	HYPH
cord-009376-a35a92gh	323	12	PCR	PCR	NNP
cord-009376-a35a92gh	323	13	assays	assay	NNS
cord-009376-a35a92gh	323	14	have	have	VBP
cord-009376-a35a92gh	323	15	been	be	VBN
cord-009376-a35a92gh	323	16	designed	design	VBN
cord-009376-a35a92gh	323	17	to	to	TO
cord-009376-a35a92gh	323	18	detect	detect	VB
cord-009376-a35a92gh	323	19	and	and	CC
cord-009376-a35a92gh	323	20	Ž	ž	NN
cord-009376-a35a92gh	323	21	.	.	.
cord-009376-a35a92gh	324	1	quantify	quantify	VB
cord-009376-a35a92gh	324	2	residual	residual	JJ
cord-009376-a35a92gh	324	3	DNA	DNA	NNP
cord-009376-a35a92gh	324	4	Table	Table	NNP
cord-009376-a35a92gh	324	5	8	8	CD
cord-009376-a35a92gh	324	6	.	.	.
cord-009376-a35a92gh	325	1	The	the	DT
cord-009376-a35a92gh	325	2	quantification	quantification	NN
cord-009376-a35a92gh	325	3	limit	limit	NN
cord-009376-a35a92gh	325	4	of	of	IN
cord-009376-a35a92gh	325	5	residual	residual	JJ
cord-009376-a35a92gh	325	6	DNA	dna	NN
cord-009376-a35a92gh	325	7	Q	Q	NNP
cord-009376-a35a92gh	325	8	-	-	HYPH
cord-009376-a35a92gh	325	9	PCR	PCR	NNP
cord-009376-a35a92gh	325	10	assays	assay	NNS
cord-009376-a35a92gh	325	11	are	be	VBP
cord-009376-a35a92gh	325	12	usually	usually	RB
cord-009376-a35a92gh	325	13	within	within	IN
cord-009376-a35a92gh	325	14	the	the	DT
cord-009376-a35a92gh	325	15	5	5	CD
cord-009376-a35a92gh	325	16	pg	pg	NN
cord-009376-a35a92gh	325	17	per	per	IN
cord-009376-a35a92gh	325	18	reaction	reaction	NN
cord-009376-a35a92gh	325	19	or	or	CC
cord-009376-a35a92gh	325	20	50	50	CD
cord-009376-a35a92gh	325	21	᎐	᎐	CD
cord-009376-a35a92gh	325	22	100	100	CD
cord-009376-a35a92gh	325	23	pg	pg	NN
cord-009376-a35a92gh	325	24	Ž	ž	NN
cord-009376-a35a92gh	325	25	Slot	slot	NN
cord-009376-a35a92gh	325	26	-	-	HYPH
cord-009376-a35a92gh	325	27	blot	blot	NN
cord-009376-a35a92gh	325	28	technique	technique	NN
cord-009376-a35a92gh	325	29	using	use	VBG
cord-009376-a35a92gh	325	30	P	p	NN
cord-009376-a35a92gh	325	31	-	-	HYPH
cord-009376-a35a92gh	325	32	radiolabeled	radiolabele	VBN
cord-009376-a35a92gh	325	33	probe	probe	NN
cord-009376-a35a92gh	325	34	ge-	ge-	NN
cord-009376-a35a92gh	325	35	.	.	.
cord-009376-a35a92gh	326	1	nomic	nomic	NNP
cord-009376-a35a92gh	326	2	DNA	DNA	NNP
cord-009376-a35a92gh	326	3	,	,	,
cord-009376-a35a92gh	326	4	hybridisation	hybridisation	NN
cord-009376-a35a92gh	326	5	and	and	CC
cord-009376-a35a92gh	326	6	autoradiography	autoradiography	NN
cord-009376-a35a92gh	326	7	.	.	.
cord-009376-a35a92gh	326	8	.	.	.
cord-009376-a35a92gh	327	1	10	10	CD
cord-009376-a35a92gh	328	1	᎐	᎐	CD
cord-009376-a35a92gh	328	2	20	20	CD
cord-009376-a35a92gh	328	3	genome	genome	NN
cord-009376-a35a92gh	328	4	equivalents	equivalent	NNS
cord-009376-a35a92gh	328	5	per	per	IN
cord-009376-a35a92gh	328	6	ml	ml	NN
cord-009376-a35a92gh	328	7	.	.	.
cord-009376-a35a92gh	329	1	This	this	DT
cord-009376-a35a92gh	329	2	sensitivity	sensitivity	NN
cord-009376-a35a92gh	329	3	is	be	VBZ
cord-009376-a35a92gh	329	4	expected	expect	VBN
cord-009376-a35a92gh	329	5	when	when	WRB
cord-009376-a35a92gh	329	6	using	use	VBG
cord-009376-a35a92gh	329	7	a	a	DT
cord-009376-a35a92gh	329	8	target	target	NN
cord-009376-a35a92gh	329	9	such	such	JJ
cord-009376-a35a92gh	329	10	as	as	IN
cord-009376-a35a92gh	329	11	the	the	DT
cord-009376-a35a92gh	329	12	beta	beta	JJ
cord-009376-a35a92gh	329	13	actin	actin	NN
cord-009376-a35a92gh	329	14	gene	gene	NN
cord-009376-a35a92gh	329	15	as	as	IN
cord-009376-a35a92gh	329	16	the	the	DT
cord-009376-a35a92gh	329	17	target	target	NN
cord-009376-a35a92gh	329	18	.	.	.
cord-009376-a35a92gh	330	1	Sensitivity	sensitivity	NN
cord-009376-a35a92gh	330	2	can	can	MD
cord-009376-a35a92gh	330	3	sometimes	sometimes	RB
cord-009376-a35a92gh	330	4	be	be	VB
cord-009376-a35a92gh	330	5	increased	increase	VBN
cord-009376-a35a92gh	330	6	if	if	IN
cord-009376-a35a92gh	330	7	a	a	DT
cord-009376-a35a92gh	330	8	target	target	NN
cord-009376-a35a92gh	330	9	with	with	IN
cord-009376-a35a92gh	330	10	a	a	DT
cord-009376-a35a92gh	330	11	higher	high	JJR
cord-009376-a35a92gh	330	12	copy	copy	NN
cord-009376-a35a92gh	330	13	number	number	NN
cord-009376-a35a92gh	330	14	is	be	VBZ
cord-009376-a35a92gh	330	15	used	use	VBN
cord-009376-a35a92gh	330	16	,	,	,
cord-009376-a35a92gh	330	17	for	for	IN
cord-009376-a35a92gh	330	18	example	example	NN
cord-009376-a35a92gh	330	19	a	a	DT
cord-009376-a35a92gh	330	20	sequence	sequence	NN
cord-009376-a35a92gh	330	21	repeat	repeat	NN
cord-009376-a35a92gh	330	22	region	region	NN
cord-009376-a35a92gh	330	23	within	within	IN
cord-009376-a35a92gh	330	24	the	the	DT
cord-009376-a35a92gh	330	25	genome	genome	NN
cord-009376-a35a92gh	330	26	.	.	.
cord-009376-a35a92gh	331	1	We	-PRON-	PRP
cord-009376-a35a92gh	331	2	are	be	VBP
cord-009376-a35a92gh	331	3	developing	develop	VBG
cord-009376-a35a92gh	331	4	Q	Q	NNP
cord-009376-a35a92gh	331	5	-	-	HYPH
cord-009376-a35a92gh	331	6	PCR	pcr	NN
cord-009376-a35a92gh	331	7	assays	assay	NNS
cord-009376-a35a92gh	331	8	for	for	IN
cord-009376-a35a92gh	331	9	these	these	DT
cord-009376-a35a92gh	331	10	repeat	repeat	NN
cord-009376-a35a92gh	331	11	regions	region	NNS
cord-009376-a35a92gh	331	12	for	for	IN
cord-009376-a35a92gh	331	13	rodent	rodent	NN
cord-009376-a35a92gh	331	14	and	and	CC
cord-009376-a35a92gh	331	15	primate	primate	JJ
cord-009376-a35a92gh	331	16	residual	residual	JJ
cord-009376-a35a92gh	331	17	DNA	dna	NN
cord-009376-a35a92gh	331	18	to	to	TO
cord-009376-a35a92gh	331	19	obtain	obtain	VB
cord-009376-a35a92gh	331	20	sensitivity	sensitivity	NN
cord-009376-a35a92gh	331	21	in	in	IN
cord-009376-a35a92gh	331	22	the	the	DT
cord-009376-a35a92gh	331	23	fg	fg	NNP
cord-009376-a35a92gh	331	24	range	range	NN
cord-009376-a35a92gh	331	25	,	,	,
cord-009376-a35a92gh	331	26	however	however	RB
cord-009376-a35a92gh	331	27	,	,	,
cord-009376-a35a92gh	331	28	one	one	CD
cord-009376-a35a92gh	331	29	advantage	advantage	NN
cord-009376-a35a92gh	331	30	of	of	IN
cord-009376-a35a92gh	331	31	using	use	VBG
cord-009376-a35a92gh	331	32	a	a	DT
cord-009376-a35a92gh	331	33	gene	gene	NN
cord-009376-a35a92gh	331	34	such	such	JJ
cord-009376-a35a92gh	331	35	as	as	IN
cord-009376-a35a92gh	331	36	␤	␤	NNP
cord-009376-a35a92gh	332	1	-actin	-actin	NNP
cord-009376-a35a92gh	332	2	is	be	VBZ
cord-009376-a35a92gh	332	3	gene	gene	NN
cord-009376-a35a92gh	332	4	stability	stability	NN
cord-009376-a35a92gh	332	5	,	,	,
cord-009376-a35a92gh	332	6	as	as	IN
cord-009376-a35a92gh	332	7	this	this	DT
cord-009376-a35a92gh	332	8	target	target	NN
cord-009376-a35a92gh	332	9	should	should	MD
cord-009376-a35a92gh	332	10	not	not	RB
cord-009376-a35a92gh	332	11	undergo	undergo	VB
cord-009376-a35a92gh	332	12	copy	copy	JJ
cord-009376-a35a92gh	332	13	number	number	NN
cord-009376-a35a92gh	332	14	changes	change	NNS
cord-009376-a35a92gh	332	15	within	within	IN
cord-009376-a35a92gh	332	16	a	a	DT
cord-009376-a35a92gh	332	17	stable	stable	JJ
cord-009376-a35a92gh	332	18	cell	cell	NN
cord-009376-a35a92gh	332	19	line	line	NN
cord-009376-a35a92gh	332	20	.	.	.
cord-009376-a35a92gh	333	1	Repeat	repeat	VB
cord-009376-a35a92gh	333	2	se	se	FW
cord-009376-a35a92gh	333	3	-	-	HYPH
cord-009376-a35a92gh	333	4	Ž	ž	NN
cord-009376-a35a92gh	333	5	quences	quence	NNS
cord-009376-a35a92gh	333	6	are	be	VBP
cord-009376-a35a92gh	333	7	known	know	VBN
cord-009376-a35a92gh	333	8	to	to	TO
cord-009376-a35a92gh	333	9	be	be	VB
cord-009376-a35a92gh	333	10	less	less	RBR
cord-009376-a35a92gh	333	11	stable	stable	JJ
cord-009376-a35a92gh	334	1	Lewis	Lewis	NNP
cord-009376-a35a92gh	334	2	et	et	NNP
cord-009376-a35a92gh	334	3	al	al	NNP
cord-009376-a35a92gh	334	4	.	.	.
cord-009376-a35a92gh	335	1	,	,	,
cord-009376-a35a92gh	335	2	.	.	.
cord-009376-a35a92gh	336	1	1999b	1999b	CD
cord-009376-a35a92gh	336	2	;	;	:
cord-009376-a35a92gh	336	3	Lobachev	Lobachev	NNP
cord-009376-a35a92gh	336	4	et	et	NNP
cord-009376-a35a92gh	336	5	al	al	NNP
cord-009376-a35a92gh	336	6	.	.	.
cord-009376-a35a92gh	337	1	,	,	,
cord-009376-a35a92gh	337	2	2000	2000	CD
cord-009376-a35a92gh	337	3	and	and	CC
cord-009376-a35a92gh	337	4	may	may	MD
cord-009376-a35a92gh	337	5	undergo	undergo	VB
cord-009376-a35a92gh	337	6	significant	significant	JJ
cord-009376-a35a92gh	337	7	changes	change	NNS
cord-009376-a35a92gh	337	8	in	in	IN
cord-009376-a35a92gh	337	9	copy	copy	NN
cord-009376-a35a92gh	337	10	number	number	NN
cord-009376-a35a92gh	337	11	on	on	IN
cord-009376-a35a92gh	337	12	the	the	DT
cord-009376-a35a92gh	337	13	genome	genome	NN
cord-009376-a35a92gh	337	14	,	,	,
cord-009376-a35a92gh	337	15	and	and	CC
cord-009376-a35a92gh	337	16	therefore	therefore	RB
cord-009376-a35a92gh	337	17	have	have	VBP
cord-009376-a35a92gh	337	18	the	the	DT
cord-009376-a35a92gh	337	19	potential	potential	NN
cord-009376-a35a92gh	337	20	to	to	TO
cord-009376-a35a92gh	337	21	obscure	obscure	VB
cord-009376-a35a92gh	337	22	the	the	DT
cord-009376-a35a92gh	337	23	residual	residual	JJ
cord-009376-a35a92gh	337	24	DNA	dna	NN
cord-009376-a35a92gh	337	25	data	datum	NNS
cord-009376-a35a92gh	337	26	if	if	IN
cord-009376-a35a92gh	337	27	a	a	DT
cord-009376-a35a92gh	337	28	shift	shift	NN
cord-009376-a35a92gh	337	29	in	in	IN
cord-009376-a35a92gh	337	30	target	target	NN
cord-009376-a35a92gh	337	31	copy	copy	NN
cord-009376-a35a92gh	337	32	number	number	NN
cord-009376-a35a92gh	337	33	occurs	occur	VBZ
cord-009376-a35a92gh	337	34	during	during	IN
cord-009376-a35a92gh	337	35	production	production	NN
cord-009376-a35a92gh	337	36	.	.	.
cord-009376-a35a92gh	338	1	For	for	IN
cord-009376-a35a92gh	338	2	these	these	DT
cord-009376-a35a92gh	338	3	reasons	reason	NNS
cord-009376-a35a92gh	338	4	,	,	,
cord-009376-a35a92gh	338	5	the	the	DT
cord-009376-a35a92gh	338	6	choice	choice	NN
cord-009376-a35a92gh	338	7	of	of	IN
cord-009376-a35a92gh	338	8	PCR	PCR	NNP
cord-009376-a35a92gh	338	9	target	target	NN
cord-009376-a35a92gh	338	10	is	be	VBZ
cord-009376-a35a92gh	338	11	important	important	JJ
cord-009376-a35a92gh	338	12	and	and	CC
cord-009376-a35a92gh	338	13	the	the	DT
cord-009376-a35a92gh	338	14	use	use	NN
cord-009376-a35a92gh	338	15	of	of	IN
cord-009376-a35a92gh	338	16	repeat	repeat	NN
cord-009376-a35a92gh	338	17	regions	region	NNS
cord-009376-a35a92gh	338	18	may	may	MD
cord-009376-a35a92gh	338	19	not	not	RB
cord-009376-a35a92gh	338	20	be	be	VB
cord-009376-a35a92gh	338	21	necessary	necessary	JJ
cord-009376-a35a92gh	338	22	,	,	,
cord-009376-a35a92gh	338	23	when	when	WRB
cord-009376-a35a92gh	338	24	sensitivity	sensitivity	NN
cord-009376-a35a92gh	338	25	to	to	IN
cord-009376-a35a92gh	338	26	pg	pg	NN
cord-009376-a35a92gh	338	27	levels	level	NNS
cord-009376-a35a92gh	338	28	is	be	VBZ
cord-009376-a35a92gh	338	29	required	require	VBN
cord-009376-a35a92gh	338	30	.	.	.
cord-009376-a35a92gh	339	1	Besides	besides	IN
cord-009376-a35a92gh	339	2	the	the	DT
cord-009376-a35a92gh	339	3	qualification	qualification	NN
cord-009376-a35a92gh	339	4	of	of	IN
cord-009376-a35a92gh	339	5	final	final	JJ
cord-009376-a35a92gh	339	6	products	product	NNS
cord-009376-a35a92gh	339	7	for	for	IN
cord-009376-a35a92gh	339	8	batch	batch	NN
cord-009376-a35a92gh	339	9	release	release	NN
cord-009376-a35a92gh	339	10	,	,	,
cord-009376-a35a92gh	339	11	residual	residual	JJ
cord-009376-a35a92gh	339	12	DNA	dna	NN
cord-009376-a35a92gh	339	13	assays	assay	NNS
cord-009376-a35a92gh	339	14	are	be	VBP
cord-009376-a35a92gh	339	15	used	use	VBN
cord-009376-a35a92gh	339	16	to	to	TO
cord-009376-a35a92gh	339	17	demonstrate	demonstrate	VB
cord-009376-a35a92gh	339	18	clearance	clearance	NN
cord-009376-a35a92gh	339	19	of	of	IN
cord-009376-a35a92gh	339	20	host	host	NN
cord-009376-a35a92gh	339	21	cell	cell	NN
cord-009376-a35a92gh	339	22	DNA	dna	NN
cord-009376-a35a92gh	339	23	during	during	IN
cord-009376-a35a92gh	339	24	process	process	NN
cord-009376-a35a92gh	339	25	validation	validation	NN
cord-009376-a35a92gh	339	26	.	.	.
cord-009376-a35a92gh	340	1	In	in	IN
cord-009376-a35a92gh	340	2	addition	addition	NN
cord-009376-a35a92gh	340	3	,	,	,
cord-009376-a35a92gh	340	4	since	since	IN
cord-009376-a35a92gh	340	5	some	some	DT
cord-009376-a35a92gh	340	6	test	test	NN
cord-009376-a35a92gh	340	7	samples	sample	NNS
cord-009376-a35a92gh	340	8	are	be	VBP
cord-009376-a35a92gh	340	9	most	most	RBS
cord-009376-a35a92gh	340	10	likely	likely	JJ
cord-009376-a35a92gh	340	11	to	to	TO
cord-009376-a35a92gh	340	12	contain	contain	VB
cord-009376-a35a92gh	340	13	detectable	detectable	JJ
cord-009376-a35a92gh	340	14	levels	level	NNS
cord-009376-a35a92gh	340	15	of	of	IN
cord-009376-a35a92gh	340	16	residual	residual	JJ
cord-009376-a35a92gh	340	17	DNA	dna	NN
cord-009376-a35a92gh	340	18	,	,	,
cord-009376-a35a92gh	340	19	another	another	DT
cord-009376-a35a92gh	340	20	appropriate	appropriate	JJ
cord-009376-a35a92gh	340	21	internal	internal	JJ
cord-009376-a35a92gh	340	22	control	control	NN
cord-009376-a35a92gh	340	23	DNA	dna	NN
cord-009376-a35a92gh	340	24	that	that	WDT
cord-009376-a35a92gh	340	25	is	be	VBZ
cord-009376-a35a92gh	340	26	unrelated	unrelated	JJ
cord-009376-a35a92gh	340	27	to	to	IN
cord-009376-a35a92gh	340	28	the	the	DT
cord-009376-a35a92gh	340	29	host	host	NN
cord-009376-a35a92gh	340	30	cell	cell	NN
cord-009376-a35a92gh	340	31	and	and	CC
cord-009376-a35a92gh	340	32	test	test	NN
cord-009376-a35a92gh	340	33	sample	sample	NN
cord-009376-a35a92gh	340	34	is	be	VBZ
cord-009376-a35a92gh	340	35	spiked	spike	VBN
cord-009376-a35a92gh	340	36	into	into	IN
cord-009376-a35a92gh	340	37	the	the	DT
cord-009376-a35a92gh	340	38	sample	sample	NN
cord-009376-a35a92gh	340	39	.	.	.
cord-009376-a35a92gh	341	1	The	the	DT
cord-009376-a35a92gh	341	2	recovery	recovery	NN
cord-009376-a35a92gh	341	3	of	of	IN
cord-009376-a35a92gh	341	4	the	the	DT
cord-009376-a35a92gh	341	5	internal	internal	JJ
cord-009376-a35a92gh	341	6	control	control	NN
cord-009376-a35a92gh	341	7	is	be	VBZ
cord-009376-a35a92gh	341	8	quantified	quantify	VBN
cord-009376-a35a92gh	341	9	using	use	VBG
cord-009376-a35a92gh	341	10	a	a	DT
cord-009376-a35a92gh	341	11	PCR	PCR	NNP
cord-009376-a35a92gh	341	12	reaction	reaction	NN
cord-009376-a35a92gh	341	13	specific	specific	JJ
cord-009376-a35a92gh	341	14	for	for	IN
cord-009376-a35a92gh	341	15	the	the	DT
cord-009376-a35a92gh	341	16	unrelated	unrelated	JJ
cord-009376-a35a92gh	341	17	target	target	NN
cord-009376-a35a92gh	341	18	DNA	dna	NN
cord-009376-a35a92gh	341	19	.	.	.
cord-009376-a35a92gh	342	1	It	-PRON-	PRP
cord-009376-a35a92gh	342	2	is	be	VBZ
cord-009376-a35a92gh	342	3	important	important	JJ
cord-009376-a35a92gh	342	4	that	that	IN
cord-009376-a35a92gh	342	5	the	the	DT
cord-009376-a35a92gh	342	6	internal	internal	JJ
cord-009376-a35a92gh	342	7	control	control	NN
cord-009376-a35a92gh	342	8	target	target	NN
cord-009376-a35a92gh	342	9	and	and	CC
cord-009376-a35a92gh	342	10	test	test	VB
cord-009376-a35a92gh	342	11	sample	sample	NN
cord-009376-a35a92gh	342	12	target	target	NN
cord-009376-a35a92gh	342	13	do	do	VBP
cord-009376-a35a92gh	342	14	not	not	RB
cord-009376-a35a92gh	342	15	cross	cross	VB
cord-009376-a35a92gh	342	16	-	-	JJ
cord-009376-a35a92gh	342	17	react	react	VB
cord-009376-a35a92gh	342	18	in	in	IN
cord-009376-a35a92gh	342	19	terms	term	NNS
cord-009376-a35a92gh	342	20	of	of	IN
cord-009376-a35a92gh	342	21	sequence	sequence	NN
cord-009376-a35a92gh	342	22	specificity	specificity	NN
cord-009376-a35a92gh	342	23	.	.	.
cord-009376-a35a92gh	343	1	This	this	DT
cord-009376-a35a92gh	343	2	simplifies	simplify	VBZ
cord-009376-a35a92gh	343	3	DNA	dna	NN
cord-009376-a35a92gh	343	4	recovery	recovery	NN
cord-009376-a35a92gh	343	5	calculations	calculation	NNS
cord-009376-a35a92gh	343	6	for	for	IN
cord-009376-a35a92gh	343	7	each	each	DT
cord-009376-a35a92gh	343	8	individual	individual	JJ
cord-009376-a35a92gh	343	9	test	test	NN
cord-009376-a35a92gh	343	10	sample	sample	NN
cord-009376-a35a92gh	343	11	.	.	.
cord-009376-a35a92gh	344	1	Q	Q	NNP
cord-009376-a35a92gh	344	2	-	-	HYPH
cord-009376-a35a92gh	344	3	PCR	PCR	NNP
cord-009376-a35a92gh	344	4	for	for	IN
cord-009376-a35a92gh	344	5	residual	residual	JJ
cord-009376-a35a92gh	344	6	DNA	dna	NN
cord-009376-a35a92gh	344	7	is	be	VBZ
cord-009376-a35a92gh	344	8	far	far	RB
cord-009376-a35a92gh	344	9	more	more	RBR
cord-009376-a35a92gh	344	10	robust	robust	JJ
cord-009376-a35a92gh	344	11	than	than	IN
cord-009376-a35a92gh	344	12	conventional	conventional	JJ
cord-009376-a35a92gh	344	13	hybridisation	hybridisation	NN
cord-009376-a35a92gh	344	14	methods	method	NNS
cord-009376-a35a92gh	344	15	.	.	.
cord-009376-a35a92gh	345	1	For	for	IN
cord-009376-a35a92gh	345	2	example	example	NN
cord-009376-a35a92gh	345	3	,	,	,
cord-009376-a35a92gh	345	4	Q	Q	NNP
cord-009376-a35a92gh	345	5	-	-	HYPH
cord-009376-a35a92gh	345	6	PCR	PCR	NNP
cord-009376-a35a92gh	345	7	is	be	VBZ
cord-009376-a35a92gh	345	8	more	more	RBR
cord-009376-a35a92gh	345	9	precise	precise	JJ
cord-009376-a35a92gh	345	10	and	and	CC
cord-009376-a35a92gh	345	11	has	have	VBZ
cord-009376-a35a92gh	345	12	a	a	DT
cord-009376-a35a92gh	345	13	larger	large	JJR
cord-009376-a35a92gh	345	14	dymanic	dymanic	JJ
cord-009376-a35a92gh	345	15	range	range	NN
cord-009376-a35a92gh	345	16	than	than	IN
cord-009376-a35a92gh	345	17	conventional	conventional	JJ
cord-009376-a35a92gh	345	18	hybridisation	hybridisation	NN
cord-009376-a35a92gh	345	19	Ž	ž	NN
cord-009376-a35a92gh	345	20	.	.	.
cord-009376-a35a92gh	346	1	methods	method	NNS
cord-009376-a35a92gh	347	1	Smith	Smith	NNP
cord-009376-a35a92gh	347	2	et	et	NNP
cord-009376-a35a92gh	347	3	al	al	NNP
cord-009376-a35a92gh	347	4	.	.	NNP
cord-009376-a35a92gh	347	5	,	,	,
cord-009376-a35a92gh	347	6	1999	1999	CD
cord-009376-a35a92gh	347	7	.	.	.
cord-009376-a35a92gh	348	1	Problems	problem	NNS
cord-009376-a35a92gh	348	2	with	with	IN
cord-009376-a35a92gh	348	3	standard	standard	JJ
cord-009376-a35a92gh	348	4	curves	curve	NNS
cord-009376-a35a92gh	348	5	can	can	MD
cord-009376-a35a92gh	348	6	be	be	VB
cord-009376-a35a92gh	348	7	encountered	encounter	VBN
cord-009376-a35a92gh	348	8	when	when	WRB
cord-009376-a35a92gh	348	9	performing	perform	VBG
cord-009376-a35a92gh	348	10	linear	linear	JJ
cord-009376-a35a92gh	348	11	regression	regression	NN
cord-009376-a35a92gh	348	12	with	with	IN
cord-009376-a35a92gh	348	13	conventional	conventional	JJ
cord-009376-a35a92gh	348	14	hybridisation	hybridisation	NN
cord-009376-a35a92gh	348	15	autoradiography	autoradiography	NN
cord-009376-a35a92gh	348	16	.	.	.
cord-009376-a35a92gh	349	1	This	this	DT
cord-009376-a35a92gh	349	2	is	be	VBZ
cord-009376-a35a92gh	349	3	due	due	JJ
cord-009376-a35a92gh	349	4	to	to	IN
cord-009376-a35a92gh	349	5	the	the	DT
cord-009376-a35a92gh	349	6	fact	fact	NN
cord-009376-a35a92gh	349	7	that	that	IN
cord-009376-a35a92gh	349	8	X	x	NN
cord-009376-a35a92gh	349	9	-	-	NN
cord-009376-a35a92gh	349	10	ray	ray	NN
cord-009376-a35a92gh	349	11	films	film	NNS
cord-009376-a35a92gh	349	12	have	have	VBP
cord-009376-a35a92gh	349	13	a	a	DT
cord-009376-a35a92gh	349	14	threshold	threshold	NN
cord-009376-a35a92gh	349	15	for	for	IN
cord-009376-a35a92gh	349	16	both	both	DT
cord-009376-a35a92gh	349	17	response	response	NN
cord-009376-a35a92gh	349	18	and	and	CC
cord-009376-a35a92gh	349	19	sensitivity	sensitivity	NN
cord-009376-a35a92gh	349	20	,	,	,
cord-009376-a35a92gh	349	21	resulting	result	VBG
cord-009376-a35a92gh	349	22	in	in	IN
cord-009376-a35a92gh	349	23	a	a	DT
cord-009376-a35a92gh	349	24	non	non	JJ
cord-009376-a35a92gh	349	25	-	-	JJ
cord-009376-a35a92gh	349	26	linear	linear	JJ
cord-009376-a35a92gh	349	27	response	response	NN
cord-009376-a35a92gh	349	28	Ž	ž	NN
cord-009376-a35a92gh	349	29	.	.	.
cord-009376-a35a92gh	350	1	to	to	IN
cord-009376-a35a92gh	350	2	the	the	DT
cord-009376-a35a92gh	350	3	hybridisation	hybridisation	NN
cord-009376-a35a92gh	350	4	signal	signal	NN
cord-009376-a35a92gh	350	5	Cornett	Cornett	NNP
cord-009376-a35a92gh	350	6	et	et	NNP
cord-009376-a35a92gh	350	7	al	al	NNP
cord-009376-a35a92gh	350	8	.	.	NNP
cord-009376-a35a92gh	350	9	,	,	,
cord-009376-a35a92gh	350	10	1999	1999	CD
cord-009376-a35a92gh	350	11	.	.	.
cord-009376-a35a92gh	351	1	Q	Q	NNP
cord-009376-a35a92gh	351	2	-	-	HYPH
cord-009376-a35a92gh	351	3	PCR	PCR	NNP
cord-009376-a35a92gh	351	4	on	on	IN
cord-009376-a35a92gh	351	5	the	the	DT
cord-009376-a35a92gh	351	6	other	other	JJ
cord-009376-a35a92gh	351	7	hand	hand	NN
cord-009376-a35a92gh	351	8	,	,	,
cord-009376-a35a92gh	351	9	combines	combine	VBZ
cord-009376-a35a92gh	351	10	a	a	DT
cord-009376-a35a92gh	351	11	highly	highly	RB
cord-009376-a35a92gh	351	12	linear	linear	JJ
cord-009376-a35a92gh	351	13	response	response	NN
cord-009376-a35a92gh	351	14	coupled	couple	VBN
cord-009376-a35a92gh	351	15	with	with	IN
cord-009376-a35a92gh	351	16	sensitivity	sensitivity	NN
cord-009376-a35a92gh	351	17	comparable	comparable	JJ
cord-009376-a35a92gh	351	18	to	to	IN
cord-009376-a35a92gh	351	19	hybridisation	hybridisation	NN
cord-009376-a35a92gh	351	20	assays	assay	NNS
cord-009376-a35a92gh	351	21	.	.	.
cord-009376-a35a92gh	352	1	In	in	IN
cord-009376-a35a92gh	352	2	addition	addition	NN
cord-009376-a35a92gh	352	3	,	,	,
cord-009376-a35a92gh	352	4	turnaround	turnaround	NN
cord-009376-a35a92gh	352	5	time	time	NN
cord-009376-a35a92gh	352	6	for	for	IN
cord-009376-a35a92gh	352	7	the	the	DT
cord-009376-a35a92gh	352	8	Q	Q	NNP
cord-009376-a35a92gh	352	9	-	-	HYPH
cord-009376-a35a92gh	352	10	PCR	pcr	NN
cord-009376-a35a92gh	352	11	technique	technique	NN
cord-009376-a35a92gh	352	12	is	be	VBZ
cord-009376-a35a92gh	352	13	more	more	RBR
cord-009376-a35a92gh	352	14	rapid	rapid	JJ
cord-009376-a35a92gh	352	15	,	,	,
cord-009376-a35a92gh	352	16	less	less	CC
cord-009376-a35a92gh	352	17	labour	labour	NN
cord-009376-a35a92gh	352	18	intensive	intensive	JJ
cord-009376-a35a92gh	352	19	and	and	CC
cord-009376-a35a92gh	352	20	can	can	MD
cord-009376-a35a92gh	352	21	be	be	VB
cord-009376-a35a92gh	352	22	automated	automate	VBN
cord-009376-a35a92gh	352	23	with	with	IN
cord-009376-a35a92gh	352	24	robotic	robotic	JJ
cord-009376-a35a92gh	352	25	systems	system	NNS
cord-009376-a35a92gh	352	26	to	to	TO
cord-009376-a35a92gh	352	27	remove	remove	VB
cord-009376-a35a92gh	352	28	operator	operator	NN
cord-009376-a35a92gh	352	29	error	error	NN
cord-009376-a35a92gh	352	30	during	during	IN
cord-009376-a35a92gh	352	31	sample	sample	NN
cord-009376-a35a92gh	352	32	manipulation	manipulation	NN
cord-009376-a35a92gh	352	33	.	.	.
cord-009376-a35a92gh	353	1	Q	Q	NNP
cord-009376-a35a92gh	353	2	-	-	HYPH
cord-009376-a35a92gh	353	3	PCR	PCR	NNP
cord-009376-a35a92gh	353	4	can	can	MD
cord-009376-a35a92gh	353	5	still	still	RB
cord-009376-a35a92gh	353	6	estimate	estimate	VB
cord-009376-a35a92gh	353	7	the	the	DT
cord-009376-a35a92gh	353	8	amount	amount	NN
cord-009376-a35a92gh	353	9	of	of	IN
cord-009376-a35a92gh	353	10	DNA	dna	NN
cord-009376-a35a92gh	353	11	in	in	IN
cord-009376-a35a92gh	353	12	benzonase	benzonase	NNP
cord-009376-a35a92gh	353	13	treated	treat	VBN
cord-009376-a35a92gh	353	14	products	product	NNS
cord-009376-a35a92gh	353	15	,	,	,
cord-009376-a35a92gh	353	16	for	for	IN
cord-009376-a35a92gh	353	17	example	example	NN
cord-009376-a35a92gh	353	18	,	,	,
cord-009376-a35a92gh	353	19	if	if	IN
cord-009376-a35a92gh	353	20	DNA	DNA	NNP
cord-009376-a35a92gh	353	21	is	be	VBZ
cord-009376-a35a92gh	353	22	sheared	shear	VBN
cord-009376-a35a92gh	353	23	down	down	RP
cord-009376-a35a92gh	353	24	to	to	IN
cord-009376-a35a92gh	353	25	sizes	size	NNS
cord-009376-a35a92gh	353	26	of	of	IN
cord-009376-a35a92gh	353	27	50	50	CD
cord-009376-a35a92gh	353	28	᎐	᎐	CD
cord-009376-a35a92gh	353	29	100	100	CD
cord-009376-a35a92gh	353	30	bp	bp	NNP
cord-009376-a35a92gh	353	31	.	.	.
cord-009376-a35a92gh	354	1	Q	Q	NNP
cord-009376-a35a92gh	354	2	-	-	HYPH
cord-009376-a35a92gh	354	3	PCR	PCR	NNP
cord-009376-a35a92gh	354	4	is	be	VBZ
cord-009376-a35a92gh	354	5	essential	essential	JJ
cord-009376-a35a92gh	354	6	in	in	IN
cord-009376-a35a92gh	354	7	determining	determine	VBG
cord-009376-a35a92gh	354	8	the	the	DT
cord-009376-a35a92gh	354	9	efficacy	efficacy	NN
cord-009376-a35a92gh	354	10	of	of	IN
cord-009376-a35a92gh	354	11	gene	gene	NN
cord-009376-a35a92gh	354	12	and	and	CC
cord-009376-a35a92gh	354	13	cell	cell	NN
cord-009376-a35a92gh	354	14	therapeutics	therapeutic	NNS
cord-009376-a35a92gh	354	15	.	.	.
cord-009376-a35a92gh	355	1	Moreover	moreover	RB
cord-009376-a35a92gh	355	2	,	,	,
cord-009376-a35a92gh	355	3	the	the	DT
cord-009376-a35a92gh	355	4	biodistribution	biodistribution	NN
cord-009376-a35a92gh	355	5	analysis	analysis	NN
cord-009376-a35a92gh	355	6	of	of	IN
cord-009376-a35a92gh	355	7	such	such	JJ
cord-009376-a35a92gh	355	8	novel	novel	JJ
cord-009376-a35a92gh	355	9	therapeutics	therapeutic	NNS
cord-009376-a35a92gh	355	10	is	be	VBZ
cord-009376-a35a92gh	355	11	critical	critical	JJ
cord-009376-a35a92gh	355	12	for	for	IN
cord-009376-a35a92gh	355	13	assessment	assessment	NN
cord-009376-a35a92gh	355	14	of	of	IN
cord-009376-a35a92gh	355	15	their	-PRON-	PRP$
cord-009376-a35a92gh	355	16	bio	bio	JJ
cord-009376-a35a92gh	355	17	-	-	NN
cord-009376-a35a92gh	355	18	safety	safety	NN
cord-009376-a35a92gh	355	19	and	and	CC
cord-009376-a35a92gh	355	20	for	for	IN
cord-009376-a35a92gh	355	21	meeting	meet	VBG
cord-009376-a35a92gh	355	22	the	the	DT
cord-009376-a35a92gh	355	23	increasingly	increasingly	RB
cord-009376-a35a92gh	355	24	stringent	stringent	JJ
cord-009376-a35a92gh	355	25	regulatory	regulatory	JJ
cord-009376-a35a92gh	355	26	guidelines	guideline	NNS
cord-009376-a35a92gh	355	27	.	.	.
cord-009376-a35a92gh	356	1	In	in	IN
cord-009376-a35a92gh	356	2	this	this	DT
cord-009376-a35a92gh	356	3	way	way	NN
cord-009376-a35a92gh	356	4	,	,	,
cord-009376-a35a92gh	356	5	regulatory	regulatory	JJ
cord-009376-a35a92gh	356	6	issues	issue	NNS
cord-009376-a35a92gh	356	7	concerning	concern	VBG
cord-009376-a35a92gh	356	8	infection	infection	NN
cord-009376-a35a92gh	356	9	or	or	CC
cord-009376-a35a92gh	356	10	the	the	DT
cord-009376-a35a92gh	356	11	transfer	transfer	NN
cord-009376-a35a92gh	356	12	of	of	IN
cord-009376-a35a92gh	356	13	the	the	DT
cord-009376-a35a92gh	356	14	gene	gene	NN
cord-009376-a35a92gh	356	15	to	to	IN
cord-009376-a35a92gh	356	16	normal	normal	JJ
cord-009376-a35a92gh	356	17	or	or	CC
cord-009376-a35a92gh	356	18	distal	distal	JJ
cord-009376-a35a92gh	356	19	tissues	tissue	NNS
cord-009376-a35a92gh	356	20	,	,	,
cord-009376-a35a92gh	356	21	as	as	RB
cord-009376-a35a92gh	356	22	well	well	RB
cord-009376-a35a92gh	356	23	as	as	IN
cord-009376-a35a92gh	356	24	the	the	DT
cord-009376-a35a92gh	356	25	target	target	NN
cord-009376-a35a92gh	356	26	site	site	NN
cord-009376-a35a92gh	356	27	,	,	,
cord-009376-a35a92gh	356	28	are	be	VBP
cord-009376-a35a92gh	356	29	addressed	address	VBN
cord-009376-a35a92gh	356	30	.	.	.
cord-009376-a35a92gh	357	1	In	in	IN
cord-009376-a35a92gh	357	2	addition	addition	NN
cord-009376-a35a92gh	357	3	,	,	,
cord-009376-a35a92gh	357	4	investigations	investigation	NNS
cord-009376-a35a92gh	357	5	of	of	IN
cord-009376-a35a92gh	357	6	the	the	DT
cord-009376-a35a92gh	357	7	possibility	possibility	NN
cord-009376-a35a92gh	357	8	of	of	IN
cord-009376-a35a92gh	357	9	integration	integration	NN
cord-009376-a35a92gh	357	10	or	or	CC
cord-009376-a35a92gh	357	11	expression	expression	NN
cord-009376-a35a92gh	357	12	in	in	IN
cord-009376-a35a92gh	357	13	the	the	DT
cord-009376-a35a92gh	357	14	germ	germ	NN
cord-009376-a35a92gh	357	15	line	line	NN
cord-009376-a35a92gh	357	16	are	be	VBP
cord-009376-a35a92gh	357	17	performed	perform	VBN
cord-009376-a35a92gh	357	18	.	.	.
cord-009376-a35a92gh	358	1	If	if	IN
cord-009376-a35a92gh	358	2	possible	possible	JJ
cord-009376-a35a92gh	358	3	,	,	,
cord-009376-a35a92gh	358	4	whole	whole	JJ
cord-009376-a35a92gh	358	5	organs	organ	NNS
cord-009376-a35a92gh	358	6	should	should	MD
cord-009376-a35a92gh	358	7	(	(	-LRB-
cord-009376-a35a92gh	358	8	)	)	-RRB-
cord-009376-a35a92gh	358	9	be	be	VB
cord-009376-a35a92gh	358	10	homogenised	homogenise	VBN
cord-009376-a35a92gh	358	11	,	,	,
cord-009376-a35a92gh	358	12	an	an	DT
cord-009376-a35a92gh	358	13	aliquot	aliquot	NN
cord-009376-a35a92gh	358	14	extracted	extract	VBN
cord-009376-a35a92gh	358	15	and	and	CC
cord-009376-a35a92gh	358	16	the	the	DT
cord-009376-a35a92gh	358	17	remaining	remain	VBG
cord-009376-a35a92gh	358	18	homogenate	homogenate	NNP
cord-009376-a35a92gh	358	19	archived	archive	VBN
cord-009376-a35a92gh	358	20	for	for	IN
cord-009376-a35a92gh	358	21	further	further	JJ
cord-009376-a35a92gh	358	22	analysis	analysis	NN
cord-009376-a35a92gh	358	23	.	.	.
cord-009376-a35a92gh	359	1	Homogenisation	homogenisation	NN
cord-009376-a35a92gh	359	2	of	of	IN
cord-009376-a35a92gh	359	3	whole	whole	JJ
cord-009376-a35a92gh	359	4	organs	organ	NNS
cord-009376-a35a92gh	359	5	gives	give	VBZ
cord-009376-a35a92gh	359	6	insurance	insurance	NN
cord-009376-a35a92gh	359	7	that	that	IN
cord-009376-a35a92gh	359	8	patches	patch	NNS
cord-009376-a35a92gh	359	9	or	or	CC
cord-009376-a35a92gh	359	10	localisation	localisation	NN
cord-009376-a35a92gh	359	11	of	of	IN
cord-009376-a35a92gh	359	12	infection	infection	NN
cord-009376-a35a92gh	359	13	in	in	IN
cord-009376-a35a92gh	359	14	the	the	DT
cord-009376-a35a92gh	359	15	organ	organ	NN
cord-009376-a35a92gh	359	16	can	can	MD
cord-009376-a35a92gh	359	17	be	be	VB
cord-009376-a35a92gh	359	18	detected	detect	VBN
cord-009376-a35a92gh	359	19	.	.	.
cord-009376-a35a92gh	360	1	If	if	IN
cord-009376-a35a92gh	360	2	the	the	DT
cord-009376-a35a92gh	360	3	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	360	4	is	be	VBZ
cord-009376-a35a92gh	360	5	viral	viral	JJ
cord-009376-a35a92gh	360	6	or	or	CC
cord-009376-a35a92gh	360	7	cellular	cellular	JJ
cord-009376-a35a92gh	360	8	based	base	VBN
cord-009376-a35a92gh	360	9	,	,	,
cord-009376-a35a92gh	360	10	then	then	RB
cord-009376-a35a92gh	360	11	detection	detection	NN
cord-009376-a35a92gh	360	12	of	of	IN
cord-009376-a35a92gh	360	13	viral	viral	JJ
cord-009376-a35a92gh	360	14	or	or	CC
cord-009376-a35a92gh	360	15	cellular	cellular	JJ
cord-009376-a35a92gh	360	16	RNA	RNA	NNP
cord-009376-a35a92gh	360	17	expression	expression	NN
cord-009376-a35a92gh	360	18	in	in	IN
cord-009376-a35a92gh	360	19	tissues	tissue	NNS
cord-009376-a35a92gh	360	20	of	of	IN
cord-009376-a35a92gh	360	21	concern	concern	NN
cord-009376-a35a92gh	360	22	may	may	MD
cord-009376-a35a92gh	360	23	be	be	VB
cord-009376-a35a92gh	360	24	indicative	indicative	JJ
cord-009376-a35a92gh	360	25	of	of	IN
cord-009376-a35a92gh	360	26	virus	virus	NN
cord-009376-a35a92gh	360	27	or	or	CC
cord-009376-a35a92gh	360	28	cellular	cellular	JJ
cord-009376-a35a92gh	360	29	replication	replication	NN
cord-009376-a35a92gh	360	30	in	in	IN
cord-009376-a35a92gh	360	31	these	these	DT
cord-009376-a35a92gh	360	32	tissues	tissue	NNS
cord-009376-a35a92gh	360	33	.	.	.
cord-009376-a35a92gh	361	1	Therefore	therefore	RB
cord-009376-a35a92gh	361	2	,	,	,
cord-009376-a35a92gh	361	3	the	the	DT
cord-009376-a35a92gh	361	4	assay	assay	NN
cord-009376-a35a92gh	361	5	target	target	NN
cord-009376-a35a92gh	361	6	,	,	,
cord-009376-a35a92gh	361	7	conditions	condition	NNS
cord-009376-a35a92gh	361	8	and	and	CC
cord-009376-a35a92gh	361	9	controls	control	NNS
cord-009376-a35a92gh	361	10	used	use	VBN
cord-009376-a35a92gh	361	11	in	in	IN
cord-009376-a35a92gh	361	12	the	the	DT
cord-009376-a35a92gh	361	13	biodistribution	biodistribution	NNP
cord-009376-a35a92gh	361	14	study	study	NN
cord-009376-a35a92gh	361	15	are	be	VBP
cord-009376-a35a92gh	361	16	critical	critical	JJ
cord-009376-a35a92gh	361	17	for	for	IN
cord-009376-a35a92gh	361	18	generating	generate	VBG
cord-009376-a35a92gh	361	19	the	the	DT
cord-009376-a35a92gh	361	20	highest	high	JJS
cord-009376-a35a92gh	361	21	quality	quality	NN
cord-009376-a35a92gh	361	22	data	datum	NNS
cord-009376-a35a92gh	361	23	to	to	TO
cord-009376-a35a92gh	361	24	satisfy	satisfy	VB
cord-009376-a35a92gh	361	25	regulatory	regulatory	JJ
cord-009376-a35a92gh	361	26	authorities	authority	NNS
cord-009376-a35a92gh	361	27	.	.	.
cord-009376-a35a92gh	362	1	A	a	DT
cord-009376-a35a92gh	362	2	prestudy	prestudy	NN
cord-009376-a35a92gh	362	3	will	will	MD
cord-009376-a35a92gh	362	4	be	be	VB
cord-009376-a35a92gh	362	5	most	most	RBS
cord-009376-a35a92gh	362	6	important	important	JJ
cord-009376-a35a92gh	362	7	,	,	,
cord-009376-a35a92gh	362	8	to	to	TO
cord-009376-a35a92gh	362	9	assess	assess	VB
cord-009376-a35a92gh	362	10	the	the	DT
cord-009376-a35a92gh	362	11	best	good	JJS
cord-009376-a35a92gh	362	12	conditions	condition	NNS
cord-009376-a35a92gh	362	13	that	that	WDT
cord-009376-a35a92gh	362	14	can	can	MD
cord-009376-a35a92gh	362	15	be	be	VB
cord-009376-a35a92gh	362	16	applied	apply	VBN
cord-009376-a35a92gh	362	17	in	in	IN
cord-009376-a35a92gh	362	18	the	the	DT
cord-009376-a35a92gh	362	19	overall	overall	JJ
cord-009376-a35a92gh	362	20	biodistribution	biodistribution	NN
cord-009376-a35a92gh	362	21	assay	assay	NN
cord-009376-a35a92gh	362	22	.	.	.
cord-009376-a35a92gh	363	1	Problems	problem	NNS
cord-009376-a35a92gh	363	2	that	that	WDT
cord-009376-a35a92gh	363	3	can	can	MD
cord-009376-a35a92gh	363	4	occur	occur	VB
cord-009376-a35a92gh	363	5	during	during	IN
cord-009376-a35a92gh	363	6	a	a	DT
cord-009376-a35a92gh	363	7	bio	bio	JJ
cord-009376-a35a92gh	363	8	-	-	JJ
cord-009376-a35a92gh	363	9	distribution	distribution	NN
cord-009376-a35a92gh	363	10	study	study	NN
cord-009376-a35a92gh	363	11	are	be	VBP
cord-009376-a35a92gh	363	12	dependant	dependant	JJ
cord-009376-a35a92gh	363	13	on	on	IN
cord-009376-a35a92gh	363	14	the	the	DT
cord-009376-a35a92gh	363	15	complexity	complexity	NN
cord-009376-a35a92gh	363	16	of	of	IN
cord-009376-a35a92gh	363	17	the	the	DT
cord-009376-a35a92gh	363	18	study	study	NN
cord-009376-a35a92gh	363	19	design	design	NN
cord-009376-a35a92gh	363	20	.	.	.
cord-009376-a35a92gh	364	1	Pre	pre	JJ
cord-009376-a35a92gh	364	2	-	-	NNS
cord-009376-a35a92gh	364	3	studies	study	NNS
cord-009376-a35a92gh	364	4	can	can	MD
cord-009376-a35a92gh	364	5	be	be	VB
cord-009376-a35a92gh	364	6	widely	widely	RB
cord-009376-a35a92gh	364	7	variable	variable	JJ
cord-009376-a35a92gh	364	8	depending	depend	VBG
cord-009376-a35a92gh	364	9	on	on	IN
cord-009376-a35a92gh	364	10	the	the	DT
cord-009376-a35a92gh	364	11	complexity	complexity	NN
cord-009376-a35a92gh	364	12	of	of	IN
cord-009376-a35a92gh	364	13	the	the	DT
cord-009376-a35a92gh	364	14	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	364	15	vector	vector	NN
cord-009376-a35a92gh	364	16	.	.	.
cord-009376-a35a92gh	365	1	However	however	RB
cord-009376-a35a92gh	365	2	,	,	,
cord-009376-a35a92gh	365	3	in	in	IN
cord-009376-a35a92gh	365	4	simplistic	simplistic	JJ
cord-009376-a35a92gh	365	5	terms	term	NNS
cord-009376-a35a92gh	365	6	,	,	,
cord-009376-a35a92gh	365	7	the	the	DT
cord-009376-a35a92gh	365	8	pre	pre	JJ
cord-009376-a35a92gh	365	9	-	-	NN
cord-009376-a35a92gh	365	10	study	study	NN
cord-009376-a35a92gh	365	11	can	can	MD
cord-009376-a35a92gh	365	12	be	be	VB
cord-009376-a35a92gh	365	13	used	use	VBN
cord-009376-a35a92gh	365	14	to	to	TO
cord-009376-a35a92gh	365	15	demonstrate	demonstrate	VB
cord-009376-a35a92gh	365	16	that	that	DT
cord-009376-a35a92gh	365	17	optimal	optimal	JJ
cord-009376-a35a92gh	365	18	PCR	pcr	NN
cord-009376-a35a92gh	365	19	,	,	,
cord-009376-a35a92gh	365	20	nucleic	nucleic	JJ
cord-009376-a35a92gh	365	21	acid	acid	NN
cord-009376-a35a92gh	365	22	extraction	extraction	NN
cord-009376-a35a92gh	365	23	and	and	CC
cord-009376-a35a92gh	365	24	maximum	maximum	JJ
cord-009376-a35a92gh	365	25	assay	assay	NN
cord-009376-a35a92gh	365	26	sensitivity	sensitivity	NN
cord-009376-a35a92gh	365	27	are	be	VBP
cord-009376-a35a92gh	365	28	all	all	RB
cord-009376-a35a92gh	365	29	addressed	address	VBN
cord-009376-a35a92gh	365	30	.	.	.
cord-009376-a35a92gh	366	1	For	for	IN
cord-009376-a35a92gh	366	2	high	high	JJ
cord-009376-a35a92gh	366	3	quality	quality	NN
cord-009376-a35a92gh	366	4	data	datum	NNS
cord-009376-a35a92gh	366	5	,	,	,
cord-009376-a35a92gh	366	6	it	-PRON-	PRP
cord-009376-a35a92gh	366	7	may	may	MD
cord-009376-a35a92gh	366	8	also	also	RB
cord-009376-a35a92gh	366	9	be	be	VB
cord-009376-a35a92gh	366	10	necessary	necessary	JJ
cord-009376-a35a92gh	366	11	for	for	IN
cord-009376-a35a92gh	366	12	the	the	DT
cord-009376-a35a92gh	366	13	preparation	preparation	NN
cord-009376-a35a92gh	366	14	of	of	IN
cord-009376-a35a92gh	366	15	an	an	DT
cord-009376-a35a92gh	366	16	inactivated	inactivate	VBN
cord-009376-a35a92gh	366	17	vector	vector	NN
cord-009376-a35a92gh	366	18	to	to	TO
cord-009376-a35a92gh	366	19	provide	provide	VB
cord-009376-a35a92gh	366	20	the	the	DT
cord-009376-a35a92gh	366	21	material	material	NN
cord-009376-a35a92gh	366	22	for	for	IN
cord-009376-a35a92gh	366	23	administration	administration	NN
cord-009376-a35a92gh	366	24	into	into	IN
cord-009376-a35a92gh	366	25	the	the	DT
cord-009376-a35a92gh	366	26	negative	negative	JJ
cord-009376-a35a92gh	366	27	control	control	NN
cord-009376-a35a92gh	366	28	animals	animal	NNS
cord-009376-a35a92gh	366	29	.	.	.
cord-009376-a35a92gh	367	1	This	this	DT
cord-009376-a35a92gh	367	2	will	will	MD
cord-009376-a35a92gh	367	3	help	help	VB
cord-009376-a35a92gh	367	4	control	control	VB
cord-009376-a35a92gh	367	5	for	for	IN
cord-009376-a35a92gh	367	6	the	the	DT
cord-009376-a35a92gh	367	7	presence	presence	NN
cord-009376-a35a92gh	367	8	of	of	IN
cord-009376-a35a92gh	367	9	any	any	DT
cord-009376-a35a92gh	367	10	residual	residual	JJ
cord-009376-a35a92gh	367	11	vector	vector	NN
cord-009376-a35a92gh	367	12	nucleic	nucleic	JJ
cord-009376-a35a92gh	367	13	acid	acid	NN
cord-009376-a35a92gh	367	14	that	that	WDT
cord-009376-a35a92gh	367	15	becomes	become	VBZ
cord-009376-a35a92gh	367	16	lodged	lodge	VBN
cord-009376-a35a92gh	367	17	in	in	IN
cord-009376-a35a92gh	367	18	animal	animal	NN
cord-009376-a35a92gh	367	19	organs	organ	NNS
cord-009376-a35a92gh	367	20	and	and	CC
cord-009376-a35a92gh	367	21	therefore	therefore	RB
cord-009376-a35a92gh	367	22	provide	provide	VB
cord-009376-a35a92gh	367	23	information	information	NN
cord-009376-a35a92gh	367	24	to	to	TO
cord-009376-a35a92gh	367	25	distinguish	distinguish	VB
cord-009376-a35a92gh	367	26	between	between	IN
cord-009376-a35a92gh	367	27	signals	signal	NNS
cord-009376-a35a92gh	367	28	that	that	WDT
cord-009376-a35a92gh	367	29	can	can	MD
cord-009376-a35a92gh	367	30	be	be	VB
cord-009376-a35a92gh	367	31	generated	generate	VBN
cord-009376-a35a92gh	367	32	from	from	IN
cord-009376-a35a92gh	367	33	the	the	DT
cord-009376-a35a92gh	367	34	replicating	replicate	VBG
cord-009376-a35a92gh	367	35	vector	vector	NN
cord-009376-a35a92gh	367	36	and	and	CC
cord-009376-a35a92gh	367	37	those	those	DT
cord-009376-a35a92gh	367	38	from	from	IN
cord-009376-a35a92gh	367	39	the	the	DT
cord-009376-a35a92gh	367	40	residual	residual	JJ
cord-009376-a35a92gh	367	41	nucleic	nucleic	JJ
cord-009376-a35a92gh	367	42	acid	acid	NN
cord-009376-a35a92gh	367	43	.	.	.
cord-009376-a35a92gh	368	1	In	in	IN
cord-009376-a35a92gh	368	2	addition	addition	NN
cord-009376-a35a92gh	368	3	,	,	,
cord-009376-a35a92gh	368	4	if	if	IN
cord-009376-a35a92gh	368	5	the	the	DT
cord-009376-a35a92gh	368	6	vector	vector	NN
cord-009376-a35a92gh	368	7	is	be	VBZ
cord-009376-a35a92gh	368	8	a	a	DT
cord-009376-a35a92gh	368	9	DNA	dna	NN
cord-009376-a35a92gh	368	10	virus	virus	NN
cord-009376-a35a92gh	368	11	,	,	,
cord-009376-a35a92gh	368	12	the	the	DT
cord-009376-a35a92gh	368	13	use	use	NN
cord-009376-a35a92gh	368	14	of	of	IN
cord-009376-a35a92gh	368	15	Q	Q	NNP
cord-009376-a35a92gh	368	16	-	-	HYPH
cord-009376-a35a92gh	368	17	RT	RT	NNP
cord-009376-a35a92gh	368	18	-	-	HYPH
cord-009376-a35a92gh	368	19	PCR	PCR	NNP
cord-009376-a35a92gh	368	20	with	with	IN
cord-009376-a35a92gh	368	21	the	the	DT
cord-009376-a35a92gh	368	22	incorporation	incorporation	NN
cord-009376-a35a92gh	368	23	of	of	IN
cord-009376-a35a92gh	368	24	DNase	DNase	NNP
cord-009376-a35a92gh	368	25	during	during	IN
cord-009376-a35a92gh	368	26	extraction	extraction	NN
cord-009376-a35a92gh	368	27	and	and	CC
cord-009376-a35a92gh	368	28	a	a	DT
cord-009376-a35a92gh	368	29	minus	minus	JJ
cord-009376-a35a92gh	368	30	RT	rt	NN
cord-009376-a35a92gh	368	31	reaction	reaction	NN
cord-009376-a35a92gh	368	32	control	control	NN
cord-009376-a35a92gh	368	33	will	will	MD
cord-009376-a35a92gh	368	34	help	help	VB
cord-009376-a35a92gh	368	35	distinguish	distinguish	VB
cord-009376-a35a92gh	368	36	between	between	IN
cord-009376-a35a92gh	368	37	viral	viral	JJ
cord-009376-a35a92gh	368	38	expression	expression	NN
cord-009376-a35a92gh	368	39	and	and	CC
cord-009376-a35a92gh	368	40	residual	residual	JJ
cord-009376-a35a92gh	368	41	DNA	dna	NN
cord-009376-a35a92gh	368	42	in	in	IN
cord-009376-a35a92gh	368	43	the	the	DT
cord-009376-a35a92gh	368	44	animal	animal	NN
cord-009376-a35a92gh	368	45	tissue	tissue	NN
cord-009376-a35a92gh	368	46	.	.	.
cord-009376-a35a92gh	369	1	The	the	DT
cord-009376-a35a92gh	369	2	method	method	NN
cord-009376-a35a92gh	369	3	for	for	IN
cord-009376-a35a92gh	369	4	inactivation	inactivation	NN
cord-009376-a35a92gh	369	5	of	of	IN
cord-009376-a35a92gh	369	6	the	the	DT
cord-009376-a35a92gh	369	7	viral	viral	JJ
cord-009376-a35a92gh	369	8	vector	vector	NN
cord-009376-a35a92gh	369	9	will	will	MD
cord-009376-a35a92gh	369	10	depend	depend	VB
cord-009376-a35a92gh	369	11	on	on	IN
cord-009376-a35a92gh	369	12	the	the	DT
cord-009376-a35a92gh	369	13	virus	virus	NN
cord-009376-a35a92gh	369	14	,	,	,
cord-009376-a35a92gh	369	15	and	and	CC
cord-009376-a35a92gh	369	16	may	may	MD
cord-009376-a35a92gh	369	17	be	be	VB
cord-009376-a35a92gh	369	18	either	either	CC
cord-009376-a35a92gh	369	19	UV	uv	NN
cord-009376-a35a92gh	369	20	or	or	CC
cord-009376-a35a92gh	369	21	B	b	NN
cord-009376-a35a92gh	369	22	-	-	HYPH
cord-009376-a35a92gh	369	23	propriolactone	propriolactone	NN
cord-009376-a35a92gh	369	24	treatment	treatment	NN
cord-009376-a35a92gh	369	25	.	.	.
cord-009376-a35a92gh	370	1	The	the	DT
cord-009376-a35a92gh	370	2	inactivated	inactivate	VBN
cord-009376-a35a92gh	370	3	preparation	preparation	NN
cord-009376-a35a92gh	370	4	should	should	MD
cord-009376-a35a92gh	370	5	then	then	RB
cord-009376-a35a92gh	370	6	be	be	VB
cord-009376-a35a92gh	370	7	verified	verify	VBN
cord-009376-a35a92gh	370	8	by	by	IN
cord-009376-a35a92gh	370	9	amplification	amplification	NN
cord-009376-a35a92gh	370	10	using	use	VBG
cord-009376-a35a92gh	370	11	cell	cell	NN
cord-009376-a35a92gh	370	12	culture	culture	NN
cord-009376-a35a92gh	370	13	and	and	CC
cord-009376-a35a92gh	370	14	the	the	DT
cord-009376-a35a92gh	370	15	replicating	replicate	VBG
cord-009376-a35a92gh	370	16	virus	virus	NN
cord-009376-a35a92gh	370	17	vector	vector	NN
cord-009376-a35a92gh	370	18	examined	examine	VBN
cord-009376-a35a92gh	370	19	by	by	IN
cord-009376-a35a92gh	370	20	an	an	DT
cord-009376-a35a92gh	370	21	appropriate	appropriate	JJ
cord-009376-a35a92gh	370	22	detection	detection	NN
cord-009376-a35a92gh	370	23	method	method	NN
cord-009376-a35a92gh	370	24	,	,	,
cord-009376-a35a92gh	370	25	for	for	IN
cord-009376-a35a92gh	370	26	example	example	NN
cord-009376-a35a92gh	370	27	cytopathic	cytopathic	JJ
cord-009376-a35a92gh	370	28	effect	effect	NN
cord-009376-a35a92gh	370	29	or	or	CC
cord-009376-a35a92gh	370	30	Q	q	NN
cord-009376-a35a92gh	370	31	-	-	HYPH
cord-009376-a35a92gh	370	32	PCR	PCR	NNP
cord-009376-a35a92gh	370	33	.	.	.
cord-009376-a35a92gh	371	1	Healthy	healthy	JJ
cord-009376-a35a92gh	371	2	cells	cell	NNS
cord-009376-a35a92gh	371	3	that	that	WDT
cord-009376-a35a92gh	371	4	have	have	VBP
cord-009376-a35a92gh	371	5	been	be	VBN
cord-009376-a35a92gh	371	6	treated	treat	VBN
cord-009376-a35a92gh	371	7	with	with	IN
cord-009376-a35a92gh	371	8	inactivated	inactivate	VBN
cord-009376-a35a92gh	371	9	vector	vector	NN
cord-009376-a35a92gh	371	10	can	can	MD
cord-009376-a35a92gh	371	11	be	be	VB
cord-009376-a35a92gh	371	12	passed	pass	VBN
cord-009376-a35a92gh	371	13	through	through	IN
cord-009376-a35a92gh	371	14	several	several	JJ
cord-009376-a35a92gh	371	15	times	time	NNS
cord-009376-a35a92gh	371	16	to	to	TO
cord-009376-a35a92gh	371	17	dilute	dilute	VB
cord-009376-a35a92gh	371	18	any	any	DT
cord-009376-a35a92gh	371	19	residual	residual	JJ
cord-009376-a35a92gh	371	20	inactivated	inactivate	VBN
cord-009376-a35a92gh	371	21	target	target	NN
cord-009376-a35a92gh	371	22	in	in	IN
cord-009376-a35a92gh	371	23	the	the	DT
cord-009376-a35a92gh	371	24	culture	culture	NN
cord-009376-a35a92gh	371	25	.	.	.
cord-009376-a35a92gh	372	1	Samples	sample	NNS
cord-009376-a35a92gh	372	2	of	of	IN
cord-009376-a35a92gh	372	3	cells	cell	NNS
cord-009376-a35a92gh	372	4	taken	take	VBN
cord-009376-a35a92gh	372	5	at	at	IN
cord-009376-a35a92gh	372	6	each	each	DT
cord-009376-a35a92gh	372	7	passage	passage	NN
cord-009376-a35a92gh	372	8	time	time	NN
cord-009376-a35a92gh	372	9	point	point	NN
cord-009376-a35a92gh	372	10	can	can	MD
cord-009376-a35a92gh	372	11	be	be	VB
cord-009376-a35a92gh	372	12	quantified	quantify	VBN
cord-009376-a35a92gh	372	13	by	by	IN
cord-009376-a35a92gh	372	14	Q	Q	NNP
cord-009376-a35a92gh	372	15	-	-	HYPH
cord-009376-a35a92gh	372	16	PCR	PCR	NNP
cord-009376-a35a92gh	372	17	with	with	IN
cord-009376-a35a92gh	372	18	and	and	CC
cord-009376-a35a92gh	372	19	without	without	IN
cord-009376-a35a92gh	372	20	RT	RT	NNP
cord-009376-a35a92gh	372	21	enzyme	enzyme	NN
cord-009376-a35a92gh	372	22	to	to	TO
cord-009376-a35a92gh	372	23	demonstrate	demonstrate	VB
cord-009376-a35a92gh	372	24	reduction	reduction	NN
cord-009376-a35a92gh	372	25	of	of	IN
cord-009376-a35a92gh	372	26	residual	residual	JJ
cord-009376-a35a92gh	372	27	viral	viral	JJ
cord-009376-a35a92gh	372	28	nucleic	nucleic	JJ
cord-009376-a35a92gh	372	29	acid	acid	NN
cord-009376-a35a92gh	372	30	and	and	CC
cord-009376-a35a92gh	372	31	the	the	DT
cord-009376-a35a92gh	372	32	absence	absence	NN
cord-009376-a35a92gh	372	33	of	of	IN
cord-009376-a35a92gh	372	34	low	low	JJ
cord-009376-a35a92gh	372	35	level	level	NN
cord-009376-a35a92gh	372	36	infection	infection	NN
cord-009376-a35a92gh	372	37	.	.	.
cord-009376-a35a92gh	373	1	Detection	detection	NN
cord-009376-a35a92gh	373	2	limit	limit	NN
cord-009376-a35a92gh	373	3	,	,	,
cord-009376-a35a92gh	373	4	linearity	linearity	NN
cord-009376-a35a92gh	373	5	and	and	CC
cord-009376-a35a92gh	373	6	reproducibility	reproducibility	NN
cord-009376-a35a92gh	373	7	should	should	MD
cord-009376-a35a92gh	373	8	all	all	DT
cord-009376-a35a92gh	373	9	be	be	VB
cord-009376-a35a92gh	373	10	determined	determine	VBN
cord-009376-a35a92gh	373	11	during	during	IN
cord-009376-a35a92gh	373	12	assay	assay	NN
cord-009376-a35a92gh	373	13	development	development	NN
cord-009376-a35a92gh	373	14	and	and	CC
cord-009376-a35a92gh	373	15	validation	validation	NN
cord-009376-a35a92gh	373	16	.	.	.
cord-009376-a35a92gh	374	1	Once	once	IN
cord-009376-a35a92gh	374	2	the	the	DT
cord-009376-a35a92gh	374	3	reaction	reaction	NN
cord-009376-a35a92gh	374	4	conditions	condition	NNS
cord-009376-a35a92gh	374	5	are	be	VBP
cord-009376-a35a92gh	374	6	optimal	optimal	JJ
cord-009376-a35a92gh	374	7	,	,	,
cord-009376-a35a92gh	374	8	spiked	spike	VBD
cord-009376-a35a92gh	374	9	negative	negative	JJ
cord-009376-a35a92gh	374	10	control	control	NN
cord-009376-a35a92gh	374	11	organs	organ	NNS
cord-009376-a35a92gh	374	12	are	be	VBP
cord-009376-a35a92gh	374	13	analysed	analyse	VBN
cord-009376-a35a92gh	374	14	to	to	TO
cord-009376-a35a92gh	374	15	determine	determine	VB
cord-009376-a35a92gh	374	16	the	the	DT
cord-009376-a35a92gh	374	17	lowest	low	JJS
cord-009376-a35a92gh	374	18	amount	amount	NN
cord-009376-a35a92gh	374	19	of	of	IN
cord-009376-a35a92gh	374	20	target	target	NN
cord-009376-a35a92gh	374	21	that	that	WDT
cord-009376-a35a92gh	374	22	can	can	MD
cord-009376-a35a92gh	374	23	be	be	VB
cord-009376-a35a92gh	374	24	detected	detect	VBN
cord-009376-a35a92gh	374	25	in	in	IN
cord-009376-a35a92gh	374	26	the	the	DT
cord-009376-a35a92gh	374	27	maximum	maximum	JJ
cord-009376-a35a92gh	374	28	amount	amount	NN
cord-009376-a35a92gh	374	29	of	of	IN
cord-009376-a35a92gh	374	30	test	test	NN
cord-009376-a35a92gh	374	31	sample	sample	NN
cord-009376-a35a92gh	374	32	tissue	tissue	NN
cord-009376-a35a92gh	374	33	.	.	.
cord-009376-a35a92gh	375	1	The	the	DT
cord-009376-a35a92gh	375	2	optimal	optimal	JJ
cord-009376-a35a92gh	375	3	extraction	extraction	NN
cord-009376-a35a92gh	375	4	conditions	condition	NNS
cord-009376-a35a92gh	375	5	can	can	MD
cord-009376-a35a92gh	375	6	be	be	VB
cord-009376-a35a92gh	375	7	applied	apply	VBN
cord-009376-a35a92gh	375	8	in	in	IN
cord-009376-a35a92gh	375	9	the	the	DT
cord-009376-a35a92gh	375	10	biodistribution	biodistribution	NNP
cord-009376-a35a92gh	375	11	study	study	NN
cord-009376-a35a92gh	375	12	.	.	.
cord-009376-a35a92gh	376	1	The	the	DT
cord-009376-a35a92gh	376	2	animal	animal	NN
cord-009376-a35a92gh	376	3	species	specie	NNS
cord-009376-a35a92gh	376	4	used	use	VBN
cord-009376-a35a92gh	376	5	in	in	IN
cord-009376-a35a92gh	376	6	the	the	DT
cord-009376-a35a92gh	376	7	biodistribution	biodistribution	NN
cord-009376-a35a92gh	376	8	study	study	NN
cord-009376-a35a92gh	376	9	is	be	VBZ
cord-009376-a35a92gh	376	10	extremely	extremely	RB
cord-009376-a35a92gh	376	11	important	important	JJ
cord-009376-a35a92gh	376	12	.	.	.
cord-009376-a35a92gh	377	1	The	the	DT
cord-009376-a35a92gh	377	2	species	specie	NNS
cord-009376-a35a92gh	377	3	must	must	MD
cord-009376-a35a92gh	377	4	be	be	VB
cord-009376-a35a92gh	377	5	acceptable	acceptable	JJ
cord-009376-a35a92gh	377	6	to	to	IN
cord-009376-a35a92gh	377	7	the	the	DT
cord-009376-a35a92gh	377	8	regulatory	regulatory	JJ
cord-009376-a35a92gh	377	9	authorities	authority	NNS
cord-009376-a35a92gh	377	10	for	for	IN
cord-009376-a35a92gh	377	11	use	use	NN
cord-009376-a35a92gh	377	12	in	in	IN
cord-009376-a35a92gh	377	13	safety	safety	NN
cord-009376-a35a92gh	377	14	evaluation	evaluation	NN
cord-009376-a35a92gh	377	15	studies	study	NNS
cord-009376-a35a92gh	377	16	and	and	CC
cord-009376-a35a92gh	377	17	extensive	extensive	JJ
cord-009376-a35a92gh	377	18	background	background	NN
cord-009376-a35a92gh	377	19	data	datum	NNS
cord-009376-a35a92gh	377	20	should	should	MD
cord-009376-a35a92gh	377	21	be	be	VB
cord-009376-a35a92gh	377	22	available	available	JJ
cord-009376-a35a92gh	377	23	.	.	.
cord-009376-a35a92gh	378	1	The	the	DT
cord-009376-a35a92gh	378	2	chosen	choose	VBN
cord-009376-a35a92gh	378	3	animal	animal	NN
cord-009376-a35a92gh	378	4	species	specie	NNS
cord-009376-a35a92gh	378	5	should	should	MD
cord-009376-a35a92gh	378	6	be	be	VB
cord-009376-a35a92gh	378	7	susceptible	susceptible	JJ
cord-009376-a35a92gh	378	8	to	to	IN
cord-009376-a35a92gh	378	9	infection	infection	NN
cord-009376-a35a92gh	378	10	.	.	.
cord-009376-a35a92gh	379	1	The	the	DT
cord-009376-a35a92gh	379	2	route	route	NN
cord-009376-a35a92gh	379	3	of	of	IN
cord-009376-a35a92gh	379	4	administration	administration	NN
cord-009376-a35a92gh	379	5	can	can	MD
cord-009376-a35a92gh	379	6	be	be	VB
cord-009376-a35a92gh	379	7	the	the	DT
cord-009376-a35a92gh	379	8	intended	intend	VBN
cord-009376-a35a92gh	379	9	human	human	JJ
cord-009376-a35a92gh	379	10	route	route	NN
cord-009376-a35a92gh	379	11	for	for	IN
cord-009376-a35a92gh	379	12	the	the	DT
cord-009376-a35a92gh	379	13	vector	vector	NN
cord-009376-a35a92gh	379	14	,	,	,
cord-009376-a35a92gh	379	15	however	however	RB
cord-009376-a35a92gh	379	16	,	,	,
cord-009376-a35a92gh	379	17	the	the	DT
cord-009376-a35a92gh	379	18	intravenous	intravenous	JJ
cord-009376-a35a92gh	379	19	route	route	NN
cord-009376-a35a92gh	379	20	allows	allow	VBZ
cord-009376-a35a92gh	379	21	better	well	JJR
cord-009376-a35a92gh	379	22	assessment	assessment	NN
cord-009376-a35a92gh	379	23	of	of	IN
cord-009376-a35a92gh	379	24	the	the	DT
cord-009376-a35a92gh	379	25	vector	vector	NN
cord-009376-a35a92gh	379	26	biodistribution	biodistribution	NN
cord-009376-a35a92gh	379	27	.	.	.
cord-009376-a35a92gh	380	1	Both	both	DT
cord-009376-a35a92gh	380	2	animal	animal	NN
cord-009376-a35a92gh	380	3	sexes	sex	NNS
cord-009376-a35a92gh	380	4	should	should	MD
cord-009376-a35a92gh	380	5	be	be	VB
cord-009376-a35a92gh	380	6	used	use	VBN
cord-009376-a35a92gh	380	7	in	in	IN
cord-009376-a35a92gh	380	8	the	the	DT
cord-009376-a35a92gh	380	9	study	study	NN
cord-009376-a35a92gh	380	10	and	and	CC
cord-009376-a35a92gh	380	11	the	the	DT
cord-009376-a35a92gh	380	12	administrative	administrative	JJ
cord-009376-a35a92gh	380	13	dose	dose	NN
cord-009376-a35a92gh	380	14	should	should	MD
cord-009376-a35a92gh	380	15	be	be	VB
cord-009376-a35a92gh	380	16	calculated	calculate	VBN
cord-009376-a35a92gh	380	17	using	use	VBG
cord-009376-a35a92gh	380	18	maximum	maximum	JJ
cord-009376-a35a92gh	380	19	human	human	JJ
cord-009376-a35a92gh	380	20	dose	dose	NN
cord-009376-a35a92gh	380	21	based	base	VBN
cord-009376-a35a92gh	380	22	on	on	IN
cord-009376-a35a92gh	380	23	equivalent	equivalent	JJ
cord-009376-a35a92gh	380	24	unit	unit	NN
cord-009376-a35a92gh	380	25	per	per	IN
cord-009376-a35a92gh	380	26	kilogram	kilogram	NN
cord-009376-a35a92gh	380	27	of	of	IN
cord-009376-a35a92gh	380	28	body	body	NN
cord-009376-a35a92gh	380	29	weight	weight	NN
cord-009376-a35a92gh	380	30	.	.	.
cord-009376-a35a92gh	381	1	An	an	DT
cord-009376-a35a92gh	381	2	example	example	NN
cord-009376-a35a92gh	381	3	of	of	IN
cord-009376-a35a92gh	381	4	an	an	DT
cord-009376-a35a92gh	381	5	animal	animal	NN
cord-009376-a35a92gh	381	6	study	study	NN
cord-009376-a35a92gh	381	7	design	design	NN
cord-009376-a35a92gh	381	8	to	to	TO
cord-009376-a35a92gh	381	9	examine	examine	VB
cord-009376-a35a92gh	381	10	vector	vector	NN
cord-009376-a35a92gh	381	11	biodistribution	biodistribution	NN
cord-009376-a35a92gh	381	12	could	could	MD
cord-009376-a35a92gh	381	13	be	be	VB
cord-009376-a35a92gh	381	14	as	as	IN
cord-009376-a35a92gh	381	15	fol	fol	NN
cord-009376-a35a92gh	381	16	-	-	HYPH
cord-009376-a35a92gh	381	17	Ž	ž	NN
cord-009376-a35a92gh	381	18	lows	low	NNS
cord-009376-a35a92gh	381	19	:	:	:
cord-009376-a35a92gh	382	1	group	group	NN
cord-009376-a35a92gh	382	2	A	A	NNP
cord-009376-a35a92gh	382	3	:	:	:
cord-009376-a35a92gh	382	4	high	high	JJ
cord-009376-a35a92gh	382	5	dose	dose	NN
cord-009376-a35a92gh	382	6	50	50	CD
cord-009376-a35a92gh	382	7	᎐	᎐	CD
cord-009376-a35a92gh	382	8	100	100	CD
cord-009376-a35a92gh	382	9	=	=	SYM
cord-009376-a35a92gh	382	10	human	human	JJ
cord-009376-a35a92gh	382	11	maxi-	maxi-	NN
cord-009376-a35a92gh	382	12	.	.	.
cord-009376-a35a92gh	383	1	Ž	Ž	NNP
cord-009376-a35a92gh	383	2	.	.	.
cord-009376-a35a92gh	384	1	mum	mum	UH
cord-009376-a35a92gh	384	2	;	;	:
cord-009376-a35a92gh	384	3	group	group	NNP
cord-009376-a35a92gh	384	4	B	B	NNP
cord-009376-a35a92gh	384	5	:	:	:
cord-009376-a35a92gh	384	6	low	low	JJ
cord-009376-a35a92gh	384	7	dose	dose	NN
cord-009376-a35a92gh	384	8	1	1	CD
cord-009376-a35a92gh	384	9	=	=	SYM
cord-009376-a35a92gh	384	10	human	human	JJ
cord-009376-a35a92gh	384	11	dose	dose	NN
cord-009376-a35a92gh	384	12	;	;	:
cord-009376-a35a92gh	384	13	and	and	CC
cord-009376-a35a92gh	384	14	group	group	NN
cord-009376-a35a92gh	384	15	C	C	NNP
cord-009376-a35a92gh	384	16	:	:	:
cord-009376-a35a92gh	384	17	high	high	JJ
cord-009376-a35a92gh	384	18	dose	dose	NN
cord-009376-a35a92gh	384	19	inactivated	inactivate	VBN
cord-009376-a35a92gh	384	20	.	.	.
cord-009376-a35a92gh	385	1	Each	each	DT
cord-009376-a35a92gh	385	2	group	group	NN
cord-009376-a35a92gh	385	3	contains	contain	VBZ
cord-009376-a35a92gh	385	4	three	three	CD
cord-009376-a35a92gh	385	5	female	female	JJ
cord-009376-a35a92gh	385	6	and	and	CC
cord-009376-a35a92gh	385	7	three	three	CD
cord-009376-a35a92gh	385	8	male	male	JJ
cord-009376-a35a92gh	385	9	animals	animal	NNS
cord-009376-a35a92gh	385	10	.	.	.
cord-009376-a35a92gh	386	1	At	at	IN
cord-009376-a35a92gh	386	2	time	time	NN
cord-009376-a35a92gh	386	3	points	point	VBZ
cord-009376-a35a92gh	386	4	8	8	CD
cord-009376-a35a92gh	386	5	,	,	,
cord-009376-a35a92gh	386	6	48	48	CD
cord-009376-a35a92gh	386	7	and	and	CC
cord-009376-a35a92gh	386	8	168	168	CD
cord-009376-a35a92gh	386	9	h	h	NN
cord-009376-a35a92gh	386	10	,	,	,
cord-009376-a35a92gh	386	11	one	one	CD
cord-009376-a35a92gh	386	12	animal	animal	NN
cord-009376-a35a92gh	386	13	is	be	VBZ
cord-009376-a35a92gh	386	14	killed	kill	VBN
cord-009376-a35a92gh	386	15	and	and	CC
cord-009376-a35a92gh	386	16	blood	blood	NN
cord-009376-a35a92gh	386	17	,	,	,
cord-009376-a35a92gh	386	18	lymph	lymph	NN
cord-009376-a35a92gh	386	19	nodes	node	NNS
cord-009376-a35a92gh	386	20	,	,	,
cord-009376-a35a92gh	386	21	lung	lung	NN
cord-009376-a35a92gh	386	22	,	,	,
cord-009376-a35a92gh	386	23	spleen	spleen	NNS
cord-009376-a35a92gh	386	24	,	,	,
cord-009376-a35a92gh	386	25	brain	brain	NN
cord-009376-a35a92gh	386	26	,	,	,
cord-009376-a35a92gh	386	27	kidneys	kidney	NNS
cord-009376-a35a92gh	386	28	,	,	,
cord-009376-a35a92gh	386	29	gonads	gonad	NNS
cord-009376-a35a92gh	386	30	,	,	,
cord-009376-a35a92gh	386	31	heart	heart	NN
cord-009376-a35a92gh	386	32	and	and	CC
cord-009376-a35a92gh	386	33	liver	liver	NN
cord-009376-a35a92gh	386	34	harvested	harvest	VBN
cord-009376-a35a92gh	386	35	under	under	IN
cord-009376-a35a92gh	386	36	carefully	carefully	RB
cord-009376-a35a92gh	386	37	controlled	control	VBN
cord-009376-a35a92gh	386	38	conditions	condition	NNS
cord-009376-a35a92gh	386	39	to	to	TO
cord-009376-a35a92gh	386	40	prevent	prevent	VB
cord-009376-a35a92gh	386	41	crosscontamination	crosscontamination	NN
cord-009376-a35a92gh	386	42	of	of	IN
cord-009376-a35a92gh	386	43	vector	vector	NN
cord-009376-a35a92gh	386	44	between	between	IN
cord-009376-a35a92gh	386	45	different	different	JJ
cord-009376-a35a92gh	386	46	organs	organ	NNS
cord-009376-a35a92gh	386	47	.	.	.
cord-009376-a35a92gh	387	1	Organs	organ	NNS
cord-009376-a35a92gh	387	2	should	should	MD
cord-009376-a35a92gh	387	3	be	be	VB
cord-009376-a35a92gh	387	4	either	either	CC
cord-009376-a35a92gh	387	5	snap	snap	VB
cord-009376-a35a92gh	387	6	frozen	freeze	VBN
cord-009376-a35a92gh	387	7	or	or	CC
cord-009376-a35a92gh	387	8	stored	store	VBN
cord-009376-a35a92gh	387	9	at	at	IN
cord-009376-a35a92gh	387	10	y80	y80	NN
cord-009376-a35a92gh	387	11	ЊC	ЊC	NNP
cord-009376-a35a92gh	387	12	in	in	IN
cord-009376-a35a92gh	387	13	individual	individual	JJ
cord-009376-a35a92gh	387	14	vials	vial	NNS
cord-009376-a35a92gh	387	15	for	for	IN
cord-009376-a35a92gh	387	16	Q	Q	NNP
cord-009376-a35a92gh	387	17	-	-	HYPH
cord-009376-a35a92gh	387	18	PCR	PCR	NNP
cord-009376-a35a92gh	387	19	analysis	analysis	NN
cord-009376-a35a92gh	387	20	.	.	.
cord-009376-a35a92gh	388	1	To	to	TO
cord-009376-a35a92gh	388	2	generate	generate	VB
cord-009376-a35a92gh	388	3	confidence	confidence	NN
cord-009376-a35a92gh	388	4	intervals	interval	NNS
cord-009376-a35a92gh	388	5	,	,	,
cord-009376-a35a92gh	388	6	a	a	DT
cord-009376-a35a92gh	388	7	minimum	minimum	NN
cord-009376-a35a92gh	388	8	of	of	IN
cord-009376-a35a92gh	388	9	three	three	CD
cord-009376-a35a92gh	388	10	replicated	replicate	VBN
cord-009376-a35a92gh	388	11	wells	well	NNS
cord-009376-a35a92gh	388	12	are	be	VBP
cord-009376-a35a92gh	388	13	required	require	VBN
cord-009376-a35a92gh	388	14	,	,	,
cord-009376-a35a92gh	388	15	but	but	CC
cord-009376-a35a92gh	388	16	more	more	RBR
cord-009376-a35a92gh	388	17	(	(	-LRB-
cord-009376-a35a92gh	388	18	)	)	-RRB-
cord-009376-a35a92gh	388	19	are	be	VBP
cord-009376-a35a92gh	388	20	preferred	prefer	VBN
cord-009376-a35a92gh	388	21	.	.	.
cord-009376-a35a92gh	389	1	This	this	DT
cord-009376-a35a92gh	389	2	ensures	ensure	VBZ
cord-009376-a35a92gh	389	3	that	that	IN
cord-009376-a35a92gh	389	4	results	result	NNS
cord-009376-a35a92gh	389	5	are	be	VBP
cord-009376-a35a92gh	389	6	expressed	express	VBN
cord-009376-a35a92gh	389	7	as	as	IN
cord-009376-a35a92gh	389	8	positive	positive	JJ
cord-009376-a35a92gh	389	9	or	or	CC
cord-009376-a35a92gh	389	10	negative	negative	JJ
cord-009376-a35a92gh	389	11	with	with	IN
cord-009376-a35a92gh	389	12	confidence	confidence	NN
cord-009376-a35a92gh	389	13	.	.	.
cord-009376-a35a92gh	390	1	The	the	DT
cord-009376-a35a92gh	390	2	use	use	NN
cord-009376-a35a92gh	390	3	of	of	IN
cord-009376-a35a92gh	390	4	only	only	JJ
cord-009376-a35a92gh	390	5	duplicate	duplicate	JJ
cord-009376-a35a92gh	390	6	or	or	CC
cord-009376-a35a92gh	390	7	single	single	JJ
cord-009376-a35a92gh	390	8	wells	well	NNS
cord-009376-a35a92gh	390	9	for	for	IN
cord-009376-a35a92gh	390	10	the	the	DT
cord-009376-a35a92gh	390	11	controls	control	NNS
cord-009376-a35a92gh	390	12	does	do	VBZ
cord-009376-a35a92gh	390	13	not	not	RB
cord-009376-a35a92gh	390	14	allow	allow	VB
cord-009376-a35a92gh	390	15	comparison	comparison	NN
cord-009376-a35a92gh	390	16	of	of	IN
cord-009376-a35a92gh	390	17	groups	group	NNS
cord-009376-a35a92gh	390	18	to	to	TO
cord-009376-a35a92gh	390	19	be	be	VB
cord-009376-a35a92gh	390	20	carried	carry	VBN
cord-009376-a35a92gh	390	21	out	out	RP
cord-009376-a35a92gh	390	22	accurately	accurately	RB
cord-009376-a35a92gh	390	23	,	,	,
cord-009376-a35a92gh	390	24	due	due	IN
cord-009376-a35a92gh	390	25	to	to	IN
cord-009376-a35a92gh	390	26	the	the	DT
cord-009376-a35a92gh	390	27	significant	significant	JJ
cord-009376-a35a92gh	390	28	high	high	JJ
cord-009376-a35a92gh	390	29	value	value	NN
cord-009376-a35a92gh	390	30	of	of	IN
cord-009376-a35a92gh	390	31	the	the	DT
cord-009376-a35a92gh	390	32	t	t	NN
cord-009376-a35a92gh	390	33	-	-	HYPH
cord-009376-a35a92gh	390	34	statistic	statistic	NN
cord-009376-a35a92gh	390	35	when	when	WRB
cord-009376-a35a92gh	390	36	using	use	VBG
cord-009376-a35a92gh	390	37	one	one	CD
cord-009376-a35a92gh	390	38	degree	degree	NN
cord-009376-a35a92gh	390	39	of	of	IN
cord-009376-a35a92gh	390	40	freedom	freedom	NN
cord-009376-a35a92gh	390	41	.	.	.
cord-009376-a35a92gh	391	1	However	however	RB
cord-009376-a35a92gh	391	2	,	,	,
cord-009376-a35a92gh	391	3	if	if	IN
cord-009376-a35a92gh	391	4	working	work	VBG
cord-009376-a35a92gh	391	5	to	to	IN
cord-009376-a35a92gh	391	6	a	a	DT
cord-009376-a35a92gh	391	7	limited	limited	JJ
cord-009376-a35a92gh	391	8	budget	budget	NN
cord-009376-a35a92gh	391	9	,	,	,
cord-009376-a35a92gh	391	10	performing	perform	VBG
cord-009376-a35a92gh	391	11	the	the	DT
cord-009376-a35a92gh	391	12	study	study	NN
cord-009376-a35a92gh	391	13	using	use	VBG
cord-009376-a35a92gh	391	14	duplicate	duplicate	JJ
cord-009376-a35a92gh	391	15	reaction	reaction	NN
cord-009376-a35a92gh	391	16	replicates	replicate	NNS
cord-009376-a35a92gh	391	17	can	can	MD
cord-009376-a35a92gh	391	18	allow	allow	VB
cord-009376-a35a92gh	391	19	the	the	DT
cord-009376-a35a92gh	391	20	data	datum	NNS
cord-009376-a35a92gh	391	21	to	to	TO
cord-009376-a35a92gh	391	22	be	be	VB
cord-009376-a35a92gh	391	23	expressed	express	VBN
cord-009376-a35a92gh	391	24	as	as	IN
cord-009376-a35a92gh	391	25	the	the	DT
cord-009376-a35a92gh	391	26	mean	mean	JJ
cord-009376-a35a92gh	391	27	copy	copy	NN
cord-009376-a35a92gh	391	28	number	number	NN
cord-009376-a35a92gh	391	29	within	within	IN
cord-009376-a35a92gh	391	30	a	a	DT
cord-009376-a35a92gh	391	31	specified	specify	VBN
cord-009376-a35a92gh	391	32	standard	standard	JJ
cord-009376-a35a92gh	391	33	deviation	deviation	NN
cord-009376-a35a92gh	391	34	.	.	.
cord-009376-a35a92gh	392	1	The	the	DT
cord-009376-a35a92gh	392	2	use	use	NN
cord-009376-a35a92gh	392	3	of	of	IN
cord-009376-a35a92gh	392	4	duplicate	duplicate	JJ
cord-009376-a35a92gh	392	5	wells	well	NNS
cord-009376-a35a92gh	392	6	with	with	IN
cord-009376-a35a92gh	392	7	a	a	DT
cord-009376-a35a92gh	392	8	mean	mean	JJ
cord-009376-a35a92gh	392	9	and	and	CC
cord-009376-a35a92gh	392	10	standard	standard	JJ
cord-009376-a35a92gh	392	11	deviation	deviation	NN
cord-009376-a35a92gh	392	12	allows	allow	VBZ
cord-009376-a35a92gh	392	13	the	the	DT
cord-009376-a35a92gh	392	14	generation	generation	NN
cord-009376-a35a92gh	392	15	of	of	IN
cord-009376-a35a92gh	392	16	a	a	DT
cord-009376-a35a92gh	392	17	68.2	68.2	CD
cord-009376-a35a92gh	392	18	%	%	NN
cord-009376-a35a92gh	392	19	confidence	confidence	NN
cord-009376-a35a92gh	392	20	interval	interval	NN
cord-009376-a35a92gh	392	21	.	.	.
cord-009376-a35a92gh	393	1	However	however	RB
cord-009376-a35a92gh	393	2	,	,	,
cord-009376-a35a92gh	393	3	the	the	DT
cord-009376-a35a92gh	393	4	use	use	NN
cord-009376-a35a92gh	393	5	of	of	IN
cord-009376-a35a92gh	393	6	single	single	JJ
cord-009376-a35a92gh	393	7	wells	well	NNS
cord-009376-a35a92gh	393	8	gives	give	VBZ
cord-009376-a35a92gh	393	9	no	no	DT
cord-009376-a35a92gh	393	10	information	information	NN
cord-009376-a35a92gh	393	11	on	on	IN
cord-009376-a35a92gh	393	12	well	well	RB
cord-009376-a35a92gh	393	13	-	-	HYPH
cord-009376-a35a92gh	393	14	to	to	TO
cord-009376-a35a92gh	393	15	-	-	HYPH
cord-009376-a35a92gh	393	16	well	well	UH
cord-009376-a35a92gh	393	17	reproducibility	reproducibility	NN
cord-009376-a35a92gh	393	18	and	and	CC
cord-009376-a35a92gh	393	19	should	should	MD
cord-009376-a35a92gh	393	20	only	only	RB
cord-009376-a35a92gh	393	21	be	be	VB
cord-009376-a35a92gh	393	22	used	use	VBN
cord-009376-a35a92gh	393	23	for	for	IN
cord-009376-a35a92gh	393	24	research	research	NN
cord-009376-a35a92gh	393	25	purposes	purpose	NNS
cord-009376-a35a92gh	393	26	rather	rather	RB
cord-009376-a35a92gh	393	27	than	than	IN
cord-009376-a35a92gh	393	28	bio	bio	JJ
cord-009376-a35a92gh	393	29	-	-	JJ
cord-009376-a35a92gh	393	30	safety	safety	JJ
cord-009376-a35a92gh	393	31	testing	testing	NN
cord-009376-a35a92gh	393	32	,	,	,
cord-009376-a35a92gh	393	33	where	where	WRB
cord-009376-a35a92gh	393	34	confidence	confidence	NN
cord-009376-a35a92gh	393	35	in	in	IN
cord-009376-a35a92gh	393	36	results	result	NNS
cord-009376-a35a92gh	393	37	is	be	VBZ
cord-009376-a35a92gh	393	38	essential	essential	JJ
cord-009376-a35a92gh	393	39	.	.	.
cord-009376-a35a92gh	394	1	To	to	TO
cord-009376-a35a92gh	394	2	produce	produce	VB
cord-009376-a35a92gh	394	3	an	an	DT
cord-009376-a35a92gh	394	4	accurate	accurate	JJ
cord-009376-a35a92gh	394	5	set	set	NN
cord-009376-a35a92gh	394	6	of	of	IN
cord-009376-a35a92gh	394	7	data	datum	NNS
cord-009376-a35a92gh	394	8	covering	cover	VBG
cord-009376-a35a92gh	394	9	the	the	DT
cord-009376-a35a92gh	394	10	dynamic	dynamic	JJ
cord-009376-a35a92gh	394	11	range	range	NN
cord-009376-a35a92gh	394	12	of	of	IN
cord-009376-a35a92gh	394	13	the	the	DT
cord-009376-a35a92gh	394	14	assay	assay	NN
cord-009376-a35a92gh	394	15	a	a	DT
cord-009376-a35a92gh	394	16	standard	standard	JJ
cord-009376-a35a92gh	394	17	curve	curve	NN
cord-009376-a35a92gh	394	18	with	with	IN
cord-009376-a35a92gh	394	19	a	a	DT
cord-009376-a35a92gh	394	20	minimum	minimum	NN
cord-009376-a35a92gh	394	21	of	of	IN
cord-009376-a35a92gh	394	22	four	four	CD
cord-009376-a35a92gh	394	23	or	or	CC
cord-009376-a35a92gh	394	24	five	five	CD
cord-009376-a35a92gh	394	25	standards	standard	NNS
cord-009376-a35a92gh	394	26	should	should	MD
cord-009376-a35a92gh	394	27	be	be	VB
cord-009376-a35a92gh	394	28	included	include	VBN
cord-009376-a35a92gh	394	29	.	.	.
cord-009376-a35a92gh	395	1	A	a	DT
cord-009376-a35a92gh	395	2	minimum	minimum	NN
cord-009376-a35a92gh	395	3	of	of	IN
cord-009376-a35a92gh	395	4	four	four	CD
cord-009376-a35a92gh	395	5	or	or	CC
cord-009376-a35a92gh	395	6	five	five	CD
cord-009376-a35a92gh	395	7	different	different	JJ
cord-009376-a35a92gh	395	8	10-fold	10-fold	JJ
cord-009376-a35a92gh	395	9	dilutions	dilution	NNS
cord-009376-a35a92gh	395	10	should	should	MD
cord-009376-a35a92gh	395	11	in	in	IN
cord-009376-a35a92gh	395	12	most	most	JJS
cord-009376-a35a92gh	395	13	cases	case	NNS
cord-009376-a35a92gh	395	14	achieve	achieve	VBP
cord-009376-a35a92gh	395	15	an	an	DT
cord-009376-a35a92gh	395	16	assessment	assessment	NN
cord-009376-a35a92gh	395	17	of	of	IN
cord-009376-a35a92gh	395	18	the	the	DT
cord-009376-a35a92gh	395	19	C	C	NNP
cord-009376-a35a92gh	395	20	values	value	NNS
cord-009376-a35a92gh	395	21	over	over	IN
cord-009376-a35a92gh	395	22	the	the	DT
cord-009376-a35a92gh	395	23	range	range	NN
cord-009376-a35a92gh	395	24	of	of	IN
cord-009376-a35a92gh	395	25	the	the	DT
cord-009376-a35a92gh	395	26	T	T	NNP
cord-009376-a35a92gh	395	27	assay	assay	NN
cord-009376-a35a92gh	395	28	on	on	IN
cord-009376-a35a92gh	395	29	every	every	DT
cord-009376-a35a92gh	395	30	PCR	PCR	NNP
cord-009376-a35a92gh	395	31	run	run	NN
cord-009376-a35a92gh	395	32	.	.	.
cord-009376-a35a92gh	396	1	These	these	DT
cord-009376-a35a92gh	396	2	controls	control	NNS
cord-009376-a35a92gh	396	3	are	be	VBP
cord-009376-a35a92gh	396	4	essential	essential	JJ
cord-009376-a35a92gh	396	5	since	since	IN
cord-009376-a35a92gh	396	6	some	some	DT
cord-009376-a35a92gh	396	7	of	of	IN
cord-009376-a35a92gh	396	8	the	the	DT
cord-009376-a35a92gh	396	9	animals	animal	NNS
cord-009376-a35a92gh	396	10	dosed	dose	VBN
cord-009376-a35a92gh	396	11	with	with	IN
cord-009376-a35a92gh	396	12	a	a	DT
cord-009376-a35a92gh	396	13	gene	gene	NN
cord-009376-a35a92gh	396	14	therapy	therapy	NN
cord-009376-a35a92gh	396	15	vectors	vector	NNS
cord-009376-a35a92gh	396	16	will	will	MD
cord-009376-a35a92gh	396	17	most	most	RBS
cord-009376-a35a92gh	396	18	likely	likely	RB
cord-009376-a35a92gh	396	19	contain	contain	VB
cord-009376-a35a92gh	396	20	some	some	DT
cord-009376-a35a92gh	396	21	PCR	pcr	NN
cord-009376-a35a92gh	396	22	-	-	HYPH
cord-009376-a35a92gh	396	23	positive	positive	JJ
cord-009376-a35a92gh	396	24	tissues	tissue	NNS
cord-009376-a35a92gh	396	25	.	.	.
cord-009376-a35a92gh	397	1	If	if	IN
cord-009376-a35a92gh	397	2	sentinel	sentinel	JJ
cord-009376-a35a92gh	397	3	extractions	extraction	NNS
cord-009376-a35a92gh	397	4	are	be	VBP
cord-009376-a35a92gh	397	5	not	not	RB
cord-009376-a35a92gh	397	6	included	include	VBN
cord-009376-a35a92gh	397	7	while	while	IN
cord-009376-a35a92gh	397	8	handling	handle	VBG
cord-009376-a35a92gh	397	9	positive	positive	JJ
cord-009376-a35a92gh	397	10	samples	sample	NNS
cord-009376-a35a92gh	397	11	,	,	,
cord-009376-a35a92gh	397	12	there	there	EX
cord-009376-a35a92gh	397	13	will	will	MD
cord-009376-a35a92gh	397	14	be	be	VB
cord-009376-a35a92gh	397	15	no	no	DT
cord-009376-a35a92gh	397	16	control	control	NN
cord-009376-a35a92gh	397	17	for	for	IN
cord-009376-a35a92gh	397	18	the	the	DT
cord-009376-a35a92gh	397	19	level	level	NN
cord-009376-a35a92gh	397	20	of	of	IN
cord-009376-a35a92gh	397	21	cross	cross	NN
cord-009376-a35a92gh	397	22	-	-	NN
cord-009376-a35a92gh	397	23	contamination	contamination	NN
cord-009376-a35a92gh	397	24	between	between	IN
cord-009376-a35a92gh	397	25	samples	sample	NNS
cord-009376-a35a92gh	397	26	during	during	IN
cord-009376-a35a92gh	397	27	extraction	extraction	NN
cord-009376-a35a92gh	397	28	.	.	.
cord-009376-a35a92gh	398	1	The	the	DT
cord-009376-a35a92gh	398	2	importance	importance	NN
cord-009376-a35a92gh	398	3	of	of	IN
cord-009376-a35a92gh	398	4	the	the	DT
cord-009376-a35a92gh	398	5	sentinel	sentinel	JJ
cord-009376-a35a92gh	398	6	extraction	extraction	NN
cord-009376-a35a92gh	398	7	is	be	VBZ
cord-009376-a35a92gh	398	8	highlighted	highlight	VBN
cord-009376-a35a92gh	398	9	since	since	IN
cord-009376-a35a92gh	398	10	the	the	DT
cord-009376-a35a92gh	398	11	Q	Q	NNP
cord-009376-a35a92gh	398	12	-	-	HYPH
cord-009376-a35a92gh	398	13	PCR	PCR	NNP
cord-009376-a35a92gh	398	14	reaction	reaction	NN
cord-009376-a35a92gh	398	15	sentinel	sentinel	NN
cord-009376-a35a92gh	398	16	can	can	MD
cord-009376-a35a92gh	398	17	be	be	VB
cord-009376-a35a92gh	398	18	negative	negative	JJ
cord-009376-a35a92gh	398	19	and	and	CC
cord-009376-a35a92gh	398	20	the	the	DT
cord-009376-a35a92gh	398	21	extraction	extraction	NN
cord-009376-a35a92gh	398	22	sentinels	sentinel	NNS
cord-009376-a35a92gh	398	23	positive	positive	JJ
cord-009376-a35a92gh	398	24	.	.	.
cord-009376-a35a92gh	399	1	This	this	DT
cord-009376-a35a92gh	399	2	control	control	NN
cord-009376-a35a92gh	399	3	ensures	ensure	VBZ
cord-009376-a35a92gh	399	4	recovery	recovery	NN
cord-009376-a35a92gh	399	5	of	of	IN
cord-009376-a35a92gh	399	6	test	test	NN
cord-009376-a35a92gh	399	7	animal	animal	NN
cord-009376-a35a92gh	399	8	nucleic	nucleic	JJ
cord-009376-a35a92gh	399	9	acid	acid	NN
cord-009376-a35a92gh	399	10	.	.	.
cord-009376-a35a92gh	400	1	This	this	DT
cord-009376-a35a92gh	400	2	is	be	VBZ
cord-009376-a35a92gh	400	3	necessary	necessary	JJ
cord-009376-a35a92gh	400	4	since	since	IN
cord-009376-a35a92gh	400	5	any	any	DT
cord-009376-a35a92gh	400	6	validation	validation	NN
cord-009376-a35a92gh	400	7	of	of	IN
cord-009376-a35a92gh	400	8	the	the	DT
cord-009376-a35a92gh	400	9	extraction	extraction	NN
cord-009376-a35a92gh	400	10	procedure	procedure	NN
cord-009376-a35a92gh	400	11	will	will	MD
cord-009376-a35a92gh	400	12	have	have	VB
cord-009376-a35a92gh	400	13	been	be	VBN
cord-009376-a35a92gh	400	14	performed	perform	VBN
cord-009376-a35a92gh	400	15	in	in	IN
cord-009376-a35a92gh	400	16	another	another	DT
cord-009376-a35a92gh	400	17	time	time	NN
cord-009376-a35a92gh	400	18	point	point	NN
cord-009376-a35a92gh	400	19	than	than	IN
cord-009376-a35a92gh	400	20	the	the	DT
cord-009376-a35a92gh	400	21	actual	actual	JJ
cord-009376-a35a92gh	400	22	test	test	NN
cord-009376-a35a92gh	400	23	sample	sample	NN
cord-009376-a35a92gh	400	24	assay	assay	NN
cord-009376-a35a92gh	400	25	.	.	.
cord-009376-a35a92gh	401	1	This	this	DT
cord-009376-a35a92gh	401	2	control	control	NN
cord-009376-a35a92gh	401	3	can	can	MD
cord-009376-a35a92gh	401	4	be	be	VB
cord-009376-a35a92gh	401	5	replaced	replace	VBN
cord-009376-a35a92gh	401	6	with	with	IN
cord-009376-a35a92gh	401	7	the	the	DT
cord-009376-a35a92gh	401	8	pre	pre	JJ
cord-009376-a35a92gh	401	9	-	-	JJ
cord-009376-a35a92gh	401	10	extraction	extraction	JJ
cord-009376-a35a92gh	401	11	spike	spike	NN
cord-009376-a35a92gh	401	12	recovery	recovery	NN
cord-009376-a35a92gh	401	13	control	control	NN
cord-009376-a35a92gh	401	14	mentioned	mention	VBD
cord-009376-a35a92gh	401	15	below	below	RB
cord-009376-a35a92gh	401	16	,	,	,
cord-009376-a35a92gh	401	17	if	if	IN
cord-009376-a35a92gh	401	18	the	the	DT
cord-009376-a35a92gh	401	19	target	target	NN
cord-009376-a35a92gh	401	20	is	be	VBZ
cord-009376-a35a92gh	401	21	not	not	RB
cord-009376-a35a92gh	401	22	integrated	integrate	VBN
cord-009376-a35a92gh	401	23	into	into	IN
cord-009376-a35a92gh	401	24	the	the	DT
cord-009376-a35a92gh	401	25	cellular	cellular	JJ
cord-009376-a35a92gh	401	26	DNA	dna	NN
cord-009376-a35a92gh	401	27	.	.	.
cord-009376-a35a92gh	402	1	If	if	IN
cord-009376-a35a92gh	402	2	the	the	DT
cord-009376-a35a92gh	402	3	integration	integration	NN
cord-009376-a35a92gh	402	4	of	of	IN
cord-009376-a35a92gh	402	5	vector	vector	NN
cord-009376-a35a92gh	402	6	DNA	dna	NN
cord-009376-a35a92gh	402	7	into	into	IN
cord-009376-a35a92gh	402	8	the	the	DT
cord-009376-a35a92gh	402	9	host	host	NN
cord-009376-a35a92gh	402	10	genome	genome	NN
cord-009376-a35a92gh	402	11	is	be	VBZ
cord-009376-a35a92gh	402	12	not	not	RB
cord-009376-a35a92gh	402	13	being	be	VBG
cord-009376-a35a92gh	402	14	assessed	assess	VBN
cord-009376-a35a92gh	402	15	,	,	,
cord-009376-a35a92gh	402	16	both	both	DT
cord-009376-a35a92gh	402	17	pre	pre	JJ
cord-009376-a35a92gh	402	18	-	-	NN
cord-009376-a35a92gh	402	19	extraction	extraction	JJ
cord-009376-a35a92gh	402	20	and	and	CC
cord-009376-a35a92gh	402	21	post	post	JJ
cord-009376-a35a92gh	402	22	-	-	JJ
cord-009376-a35a92gh	402	23	extraction	extraction	JJ
cord-009376-a35a92gh	402	24	spike	spike	NN
cord-009376-a35a92gh	402	25	controls	control	NNS
cord-009376-a35a92gh	402	26	to	to	TO
cord-009376-a35a92gh	402	27	quantify	quantify	VB
cord-009376-a35a92gh	402	28	the	the	DT
cord-009376-a35a92gh	402	29	percent	percent	NN
cord-009376-a35a92gh	402	30	of	of	IN
cord-009376-a35a92gh	402	31	recovery	recovery	NN
cord-009376-a35a92gh	402	32	during	during	IN
cord-009376-a35a92gh	402	33	extraction	extraction	NN
cord-009376-a35a92gh	402	34	and	and	CC
cord-009376-a35a92gh	402	35	any	any	DT
cord-009376-a35a92gh	402	36	inhibition	inhibition	NN
cord-009376-a35a92gh	402	37	during	during	IN
cord-009376-a35a92gh	402	38	PCR	PCR	NNP
cord-009376-a35a92gh	402	39	should	should	MD
cord-009376-a35a92gh	402	40	be	be	VB
cord-009376-a35a92gh	402	41	performed	perform	VBN
cord-009376-a35a92gh	402	42	.	.	.
cord-009376-a35a92gh	403	1	Even	even	RB
cord-009376-a35a92gh	403	2	if	if	IN
cord-009376-a35a92gh	403	3	a	a	DT
cord-009376-a35a92gh	403	4	pre	pre	JJ
cord-009376-a35a92gh	403	5	-	-	JJ
cord-009376-a35a92gh	403	6	study	study	VB
cord-009376-a35a92gh	403	7	nucleic	nucleic	JJ
cord-009376-a35a92gh	403	8	acid	acid	NN
cord-009376-a35a92gh	403	9	extraction	extraction	NN
cord-009376-a35a92gh	403	10	validation	validation	NN
cord-009376-a35a92gh	403	11	already	already	RB
cord-009376-a35a92gh	403	12	gives	give	VBZ
cord-009376-a35a92gh	403	13	this	this	DT
cord-009376-a35a92gh	403	14	information	information	NN
cord-009376-a35a92gh	403	15	,	,	,
cord-009376-a35a92gh	403	16	the	the	DT
cord-009376-a35a92gh	403	17	test	test	NN
cord-009376-a35a92gh	403	18	sample	sample	NN
cord-009376-a35a92gh	403	19	biodistribution	biodistribution	NN
cord-009376-a35a92gh	403	20	assay	assay	NN
cord-009376-a35a92gh	403	21	is	be	VBZ
cord-009376-a35a92gh	403	22	performed	perform	VBN
cord-009376-a35a92gh	403	23	at	at	IN
cord-009376-a35a92gh	403	24	a	a	DT
cord-009376-a35a92gh	403	25	time	time	NN
cord-009376-a35a92gh	403	26	point	point	NN
cord-009376-a35a92gh	403	27	other	other	JJ
cord-009376-a35a92gh	403	28	than	than	IN
cord-009376-a35a92gh	403	29	that	that	DT
cord-009376-a35a92gh	403	30	of	of	IN
cord-009376-a35a92gh	403	31	the	the	DT
cord-009376-a35a92gh	403	32	pre	pre	JJ
cord-009376-a35a92gh	403	33	-	-	NN
cord-009376-a35a92gh	403	34	study	study	NN
cord-009376-a35a92gh	403	35	.	.	.
cord-009376-a35a92gh	404	1	Possible	possible	JJ
cord-009376-a35a92gh	404	2	variables	variable	NNS
cord-009376-a35a92gh	404	3	between	between	IN
cord-009376-a35a92gh	404	4	those	those	DT
cord-009376-a35a92gh	404	5	time	time	NN
cord-009376-a35a92gh	404	6	points	point	NNS
cord-009376-a35a92gh	404	7	are	be	VBP
cord-009376-a35a92gh	404	8	,	,	,
cord-009376-a35a92gh	404	9	for	for	IN
cord-009376-a35a92gh	404	10	example	example	NN
cord-009376-a35a92gh	404	11	,	,	,
cord-009376-a35a92gh	404	12	variation	variation	NN
cord-009376-a35a92gh	404	13	in	in	IN
cord-009376-a35a92gh	404	14	solution	solution	NN
cord-009376-a35a92gh	404	15	batches	batch	NNS
cord-009376-a35a92gh	404	16	,	,	,
cord-009376-a35a92gh	404	17	different	different	JJ
cord-009376-a35a92gh	404	18	operators	operator	NNS
cord-009376-a35a92gh	404	19	,	,	,
cord-009376-a35a92gh	404	20	errors	error	NNS
cord-009376-a35a92gh	404	21	,	,	,
cord-009376-a35a92gh	404	22	or	or	CC
cord-009376-a35a92gh	404	23	problems	problem	NNS
cord-009376-a35a92gh	404	24	with	with	IN
cord-009376-a35a92gh	404	25	extraction	extraction	NN
cord-009376-a35a92gh	404	26	reagents	reagent	NNS
cord-009376-a35a92gh	404	27	.	.	.
cord-009376-a35a92gh	405	1	The	the	DT
cord-009376-a35a92gh	405	2	spike	spike	NN
cord-009376-a35a92gh	405	3	recovery	recovery	NN
cord-009376-a35a92gh	405	4	controls	control	NNS
cord-009376-a35a92gh	405	5	performed	perform	VBN
cord-009376-a35a92gh	405	6	at	at	IN
cord-009376-a35a92gh	405	7	the	the	DT
cord-009376-a35a92gh	405	8	time	time	NN
cord-009376-a35a92gh	405	9	of	of	IN
cord-009376-a35a92gh	405	10	testing	testing	NN
cord-009376-a35a92gh	405	11	ensure	ensure	VBP
cord-009376-a35a92gh	405	12	that	that	IN
cord-009376-a35a92gh	405	13	these	these	DT
cord-009376-a35a92gh	405	14	potential	potential	JJ
cord-009376-a35a92gh	405	15	problems	problem	NNS
cord-009376-a35a92gh	405	16	are	be	VBP
cord-009376-a35a92gh	405	17	detected	detect	VBN
cord-009376-a35a92gh	405	18	.	.	.
cord-009376-a35a92gh	406	1	IPC	IPC	NNP
cord-009376-a35a92gh	406	2	,	,	,
cord-009376-a35a92gh	406	3	also	also	RB
cord-009376-a35a92gh	406	4	known	know	VBN
cord-009376-a35a92gh	406	5	as	as	IN
cord-009376-a35a92gh	406	6	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	406	7	exogenous	exogenous	JJ
cord-009376-a35a92gh	406	8	internal	internal	JJ
cord-009376-a35a92gh	406	9	positive	positive	JJ
cord-009376-a35a92gh	406	10	control	control	NN
cord-009376-a35a92gh	406	11	reagents	reagent	NNS
cord-009376-a35a92gh	406	12	,	,	,
cord-009376-a35a92gh	406	13	can	can	MD
cord-009376-a35a92gh	406	14	be	be	VB
cord-009376-a35a92gh	406	15	included	include	VBN
cord-009376-a35a92gh	406	16	in	in	IN
cord-009376-a35a92gh	406	17	all	all	DT
cord-009376-a35a92gh	406	18	Q	Q	NNP
cord-009376-a35a92gh	406	19	-	-	HYPH
cord-009376-a35a92gh	406	20	PCR	pcr	NN
cord-009376-a35a92gh	406	21	master	master	NN
cord-009376-a35a92gh	406	22	mixes	mix	NNS
cord-009376-a35a92gh	406	23	.	.	.
cord-009376-a35a92gh	407	1	This	this	DT
cord-009376-a35a92gh	407	2	control	control	NN
cord-009376-a35a92gh	407	3	can	can	MD
cord-009376-a35a92gh	407	4	replace	replace	VB
cord-009376-a35a92gh	407	5	post	post	JJ
cord-009376-a35a92gh	407	6	-	-	JJ
cord-009376-a35a92gh	407	7	extraction	extraction	JJ
cord-009376-a35a92gh	407	8	spike	spike	NN
cord-009376-a35a92gh	407	9	controls	control	NNS
cord-009376-a35a92gh	407	10	,	,	,
cord-009376-a35a92gh	407	11	since	since	IN
cord-009376-a35a92gh	407	12	PCR	PCR	NNP
cord-009376-a35a92gh	407	13	inhibition	inhibition	NN
cord-009376-a35a92gh	407	14	is	be	VBZ
cord-009376-a35a92gh	407	15	detected	detect	VBN
cord-009376-a35a92gh	407	16	with	with	IN
cord-009376-a35a92gh	407	17	IPC	IPC	NNP
cord-009376-a35a92gh	407	18	reagents	reagent	NNS
cord-009376-a35a92gh	407	19	.	.	.
cord-009376-a35a92gh	408	1	IPC	IPC	NNP
cord-009376-a35a92gh	408	2	is	be	VBZ
cord-009376-a35a92gh	408	3	a	a	DT
cord-009376-a35a92gh	408	4	set	set	NN
cord-009376-a35a92gh	408	5	of	of	IN
cord-009376-a35a92gh	408	6	primers	primer	NNS
cord-009376-a35a92gh	408	7	and	and	CC
cord-009376-a35a92gh	408	8	probe	probe	VB
cord-009376-a35a92gh	408	9	targeted	target	VBN
cord-009376-a35a92gh	408	10	to	to	IN
cord-009376-a35a92gh	408	11	a	a	DT
cord-009376-a35a92gh	408	12	non	non	JJ
cord-009376-a35a92gh	408	13	-	-	JJ
cord-009376-a35a92gh	408	14	biological	biological	JJ
cord-009376-a35a92gh	408	15	synthetic	synthetic	NN
cord-009376-a35a92gh	408	16	template	template	NN
cord-009376-a35a92gh	408	17	.	.	.
cord-009376-a35a92gh	409	1	In	in	IN
cord-009376-a35a92gh	409	2	the	the	DT
cord-009376-a35a92gh	409	3	multiplexed	multiplexed	JJ
cord-009376-a35a92gh	409	4	PCR	PCR	NNP
cord-009376-a35a92gh	409	5	reaction	reaction	NN
cord-009376-a35a92gh	409	6	,	,	,
cord-009376-a35a92gh	409	7	IPC	IPC	NNP
cord-009376-a35a92gh	409	8	is	be	VBZ
cord-009376-a35a92gh	409	9	detected	detect	VBN
cord-009376-a35a92gh	409	10	using	use	VBG
cord-009376-a35a92gh	409	11	a	a	DT
cord-009376-a35a92gh	409	12	VIC	VIC	NNP
cord-009376-a35a92gh	409	13	-	-	HYPH
cord-009376-a35a92gh	409	14	labelled	label	VBN
cord-009376-a35a92gh	409	15	probe	probe	NN
cord-009376-a35a92gh	409	16	and	and	CC
cord-009376-a35a92gh	409	17	the	the	DT
cord-009376-a35a92gh	409	18	viralrcell	viralrcell	JJ
cord-009376-a35a92gh	409	19	target	target	NN
cord-009376-a35a92gh	409	20	nucleic	nucleic	JJ
cord-009376-a35a92gh	409	21	acid	acid	NN
cord-009376-a35a92gh	409	22	is	be	VBZ
cord-009376-a35a92gh	409	23	detected	detect	VBN
cord-009376-a35a92gh	409	24	using	use	VBG
cord-009376-a35a92gh	409	25	a	a	DT
cord-009376-a35a92gh	409	26	FAM	FAM	NNP
cord-009376-a35a92gh	409	27	-	-	HYPH
cord-009376-a35a92gh	409	28	labelled	label	VBN
cord-009376-a35a92gh	409	29	probe	probe	NN
cord-009376-a35a92gh	409	30	.	.	.
cord-009376-a35a92gh	410	1	IPC	IPC	NNP
cord-009376-a35a92gh	410	2	reagents	reagent	NNS
cord-009376-a35a92gh	410	3	are	be	VBP
cord-009376-a35a92gh	410	4	spiked	spike	VBN
cord-009376-a35a92gh	410	5	into	into	IN
cord-009376-a35a92gh	410	6	the	the	DT
cord-009376-a35a92gh	410	7	reactions	reaction	NNS
cord-009376-a35a92gh	410	8	to	to	TO
cord-009376-a35a92gh	410	9	distinguish	distinguish	VB
cord-009376-a35a92gh	410	10	true	true	JJ
cord-009376-a35a92gh	410	11	target	target	NN
cord-009376-a35a92gh	410	12	negatives	negative	NNS
cord-009376-a35a92gh	410	13	from	from	IN
cord-009376-a35a92gh	410	14	PCR	PCR	NNP
cord-009376-a35a92gh	410	15	inhibition	inhibition	NN
cord-009376-a35a92gh	410	16	.	.	.
cord-009376-a35a92gh	411	1	In	in	IN
cord-009376-a35a92gh	411	2	addition	addition	NN
cord-009376-a35a92gh	411	3	,	,	,
cord-009376-a35a92gh	411	4	data	datum	NNS
cord-009376-a35a92gh	411	5	generated	generate	VBN
cord-009376-a35a92gh	411	6	from	from	IN
cord-009376-a35a92gh	411	7	PCR	PCR	NNP
cord-009376-a35a92gh	411	8	-	-	HYPH
cord-009376-a35a92gh	411	9	negative	negative	JJ
cord-009376-a35a92gh	411	10	wells	well	NNS
cord-009376-a35a92gh	411	11	in	in	IN
cord-009376-a35a92gh	411	12	the	the	DT
cord-009376-a35a92gh	411	13	presence	presence	NN
cord-009376-a35a92gh	411	14	of	of	IN
cord-009376-a35a92gh	411	15	IPC	IPC	NNP
cord-009376-a35a92gh	411	16	ensure	ensure	VB
cord-009376-a35a92gh	411	17	that	that	IN
cord-009376-a35a92gh	411	18	they	-PRON-	PRP
cord-009376-a35a92gh	411	19	are	be	VBP
cord-009376-a35a92gh	411	20	truly	truly	RB
cord-009376-a35a92gh	411	21	negative	negative	JJ
cord-009376-a35a92gh	411	22	for	for	IN
cord-009376-a35a92gh	411	23	the	the	DT
cord-009376-a35a92gh	411	24	viral	viral	JJ
cord-009376-a35a92gh	411	25	target	target	NN
cord-009376-a35a92gh	411	26	,	,	,
cord-009376-a35a92gh	411	27	and	and	CC
cord-009376-a35a92gh	411	28	are	be	VBP
cord-009376-a35a92gh	411	29	not	not	RB
cord-009376-a35a92gh	411	30	due	due	JJ
cord-009376-a35a92gh	411	31	to	to	IN
cord-009376-a35a92gh	411	32	a	a	DT
cord-009376-a35a92gh	411	33	failed	fail	VBN
cord-009376-a35a92gh	411	34	amplifications	amplification	NNS
cord-009376-a35a92gh	411	35	,	,	,
cord-009376-a35a92gh	411	36	thereby	thereby	RB
cord-009376-a35a92gh	411	37	giving	give	VBG
cord-009376-a35a92gh	411	38	extra	extra	JJ
cord-009376-a35a92gh	411	39	confidence	confidence	NN
cord-009376-a35a92gh	411	40	in	in	IN
cord-009376-a35a92gh	411	41	the	the	DT
cord-009376-a35a92gh	411	42	results	result	NNS
cord-009376-a35a92gh	411	43	.	.	.
cord-009376-a35a92gh	412	1	In	in	IN
cord-009376-a35a92gh	412	2	addition	addition	NN
cord-009376-a35a92gh	412	3	to	to	IN
cord-009376-a35a92gh	412	4	viral	viral	JJ
cord-009376-a35a92gh	412	5	detection	detection	NN
cord-009376-a35a92gh	412	6	and	and	CC
cord-009376-a35a92gh	412	7	quantification	quantification	NN
cord-009376-a35a92gh	412	8	,	,	,
cord-009376-a35a92gh	412	9	Q	Q	NNP
cord-009376-a35a92gh	412	10	-	-	HYPH
cord-009376-a35a92gh	412	11	PCR	PCR	NNP
cord-009376-a35a92gh	412	12	is	be	VBZ
cord-009376-a35a92gh	412	13	widely	widely	RB
cord-009376-a35a92gh	412	14	used	use	VBN
cord-009376-a35a92gh	412	15	for	for	IN
cord-009376-a35a92gh	412	16	the	the	DT
cord-009376-a35a92gh	412	17	detection	detection	NN
cord-009376-a35a92gh	412	18	of	of	IN
cord-009376-a35a92gh	412	19	changes	change	NNS
cord-009376-a35a92gh	412	20	in	in	IN
cord-009376-a35a92gh	412	21	gene	gene	NN
cord-009376-a35a92gh	412	22	copy	copy	NN
cord-009376-a35a92gh	412	23	number	number	NN
cord-009376-a35a92gh	412	24	,	,	,
cord-009376-a35a92gh	412	25	mRNA	mrna	NN
cord-009376-a35a92gh	412	26	expression	expression	NN
cord-009376-a35a92gh	412	27	and	and	CC
cord-009376-a35a92gh	412	28	plasmid	plasmid	NN
cord-009376-a35a92gh	412	29	DNA	dna	NN
cord-009376-a35a92gh	412	30	levels	level	NNS
cord-009376-a35a92gh	412	31	.	.	.
cord-009376-a35a92gh	413	1	Q	Q	NNP
cord-009376-a35a92gh	413	2	-	-	HYPH
cord-009376-a35a92gh	413	3	PCR	PCR	NNP
cord-009376-a35a92gh	413	4	combined	combine	VBN
cord-009376-a35a92gh	413	5	with	with	IN
cord-009376-a35a92gh	413	6	DNA	dna	NN
cord-009376-a35a92gh	413	7	sequencing	sequencing	NN
cord-009376-a35a92gh	413	8	and	and	CC
cord-009376-a35a92gh	413	9	restriction	restriction	NN
cord-009376-a35a92gh	413	10	mapping	mapping	NN
cord-009376-a35a92gh	413	11	are	be	VBP
cord-009376-a35a92gh	413	12	commonly	commonly	RB
cord-009376-a35a92gh	413	13	used	use	VBN
cord-009376-a35a92gh	413	14	in	in	IN
cord-009376-a35a92gh	413	15	genetic	genetic	JJ
cord-009376-a35a92gh	413	16	stability	stability	NN
cord-009376-a35a92gh	413	17	testing	testing	NN
cord-009376-a35a92gh	413	18	.	.	.
cord-009376-a35a92gh	414	1	Examples	example	NNS
cord-009376-a35a92gh	414	2	of	of	IN
cord-009376-a35a92gh	414	3	common	common	JJ
cord-009376-a35a92gh	414	4	uses	use	NNS
cord-009376-a35a92gh	414	5	of	of	IN
cord-009376-a35a92gh	414	6	Q	Q	NNP
cord-009376-a35a92gh	414	7	-	-	HYPH
cord-009376-a35a92gh	414	8	PCR	PCR	NNP
cord-009376-a35a92gh	414	9	in	in	IN
cord-009376-a35a92gh	414	10	research	research	NN
cord-009376-a35a92gh	414	11	,	,	,
cord-009376-a35a92gh	414	12	de	de	FW
cord-009376-a35a92gh	414	13	-	-	HYPH
cord-009376-a35a92gh	414	14	Ž	Ž	NNP
cord-009376-a35a92gh	414	15	.	.	.
cord-009376-a35a92gh	415	1	velopment	velopment	NNP
cord-009376-a35a92gh	415	2	and	and	CC
cord-009376-a35a92gh	415	3	quality	quality	NN
cord-009376-a35a92gh	415	4	control	control	NN
cord-009376-a35a92gh	415	5	include	include	VBP
cord-009376-a35a92gh	415	6	:	:	:
cord-009376-a35a92gh	415	7	1	1	CD
cord-009376-a35a92gh	415	8	detec-	detec-	NN
cord-009376-a35a92gh	415	9	(	(	-LRB-
cord-009376-a35a92gh	415	10	)	)	-RRB-
cord-009376-a35a92gh	415	11	tion	tion	NN
cord-009376-a35a92gh	415	12	of	of	IN
cord-009376-a35a92gh	415	13	changes	change	NNS
cord-009376-a35a92gh	415	14	in	in	IN
cord-009376-a35a92gh	415	15	plasmid	plasmid	NN
cord-009376-a35a92gh	415	16	DNA	DNA	NNP
cord-009376-a35a92gh	415	17	copy	copy	NN
cord-009376-a35a92gh	415	18	number	number	NN
cord-009376-a35a92gh	415	19	in	in	IN
cord-009376-a35a92gh	415	20	E.	E.	NNP
cord-009376-a35a92gh	415	21	coli	coli	NNS
cord-009376-a35a92gh	415	22	and	and	CC
cord-009376-a35a92gh	415	23	yeast	yeast	NN
cord-009376-a35a92gh	415	24	cell	cell	NN
cord-009376-a35a92gh	415	25	banks	bank	NNS
cord-009376-a35a92gh	415	26	from	from	IN
cord-009376-a35a92gh	415	27	development	development	NN
cord-009376-a35a92gh	415	28	Ž	ž	NN
cord-009376-a35a92gh	415	29	.	.	.
cord-009376-a35a92gh	416	1	projects	project	NNS
cord-009376-a35a92gh	416	2	to	to	IN
cord-009376-a35a92gh	416	3	large	large	JJ
cord-009376-a35a92gh	416	4	scale	scale	NN
cord-009376-a35a92gh	416	5	production	production	NN
cord-009376-a35a92gh	416	6	;	;	:
cord-009376-a35a92gh	416	7	2	2	CD
cord-009376-a35a92gh	416	8	detection	detection	NN
cord-009376-a35a92gh	416	9	of	of	IN
cord-009376-a35a92gh	416	10	changes	change	NNS
cord-009376-a35a92gh	416	11	in	in	IN
cord-009376-a35a92gh	416	12	transgene	transgene	NN
cord-009376-a35a92gh	416	13	copy	copy	NN
cord-009376-a35a92gh	416	14	number	number	NN
cord-009376-a35a92gh	416	15	of	of	IN
cord-009376-a35a92gh	416	16	chromoso	chromoso	NNP
cord-009376-a35a92gh	416	17	-	-	HYPH
cord-009376-a35a92gh	416	18	Ž	ž	FW
cord-009376-a35a92gh	416	19	mal	mal	FW
cord-009376-a35a92gh	416	20	-	-	HYPH
cord-009376-a35a92gh	416	21	integrated	integrate	VBN
cord-009376-a35a92gh	416	22	expression	expression	NN
cord-009376-a35a92gh	416	23	cassettes	cassette	NNS
cord-009376-a35a92gh	416	24	yeast	yeast	NN
cord-009376-a35a92gh	416	25	and	and	CC
cord-009376-a35a92gh	416	26	.	.	.
cord-009376-a35a92gh	417	1	Ž	Ž	NNP
cord-009376-a35a92gh	417	2	.	.	.
cord-009376-a35a92gh	418	1	mammalian	mammalian	JJ
cord-009376-a35a92gh	418	2	cell	cell	NN
cord-009376-a35a92gh	418	3	banks	bank	NNS
cord-009376-a35a92gh	418	4	;	;	:
cord-009376-a35a92gh	418	5	3	3	CD
cord-009376-a35a92gh	418	6	copy	copy	NN
cord-009376-a35a92gh	418	7	number	number	NN
cord-009376-a35a92gh	418	8	of	of	IN
cord-009376-a35a92gh	418	9	trans	trans	NNP
cord-009376-a35a92gh	418	10	-	-	NN
cord-009376-a35a92gh	418	11	Ž	Ž	NNP
cord-009376-a35a92gh	418	12	.	.	.
cord-009376-a35a92gh	419	1	gene	gene	NNP
cord-009376-a35a92gh	419	2	mRNA	mRNA	NNP
cord-009376-a35a92gh	419	3	levels	level	NNS
cord-009376-a35a92gh	419	4	to	to	TO
cord-009376-a35a92gh	419	5	assess	assess	VB
cord-009376-a35a92gh	419	6	expression	expression	NN
cord-009376-a35a92gh	419	7	and	and	CC
cord-009376-a35a92gh	419	8	4	4	CD
cord-009376-a35a92gh	419	9	changes	change	NNS
cord-009376-a35a92gh	419	10	in	in	IN
cord-009376-a35a92gh	419	11	gene	gene	NN
cord-009376-a35a92gh	419	12	expression	expression	NN
cord-009376-a35a92gh	419	13	in	in	IN
cord-009376-a35a92gh	419	14	response	response	NN
cord-009376-a35a92gh	419	15	to	to	IN
cord-009376-a35a92gh	419	16	the	the	DT
cord-009376-a35a92gh	419	17	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	419	18	product	product	NN
cord-009376-a35a92gh	419	19	.	.	.
cord-009376-a35a92gh	420	1	Recent	recent	JJ
cord-009376-a35a92gh	420	2	approaches	approach	NNS
cord-009376-a35a92gh	420	3	to	to	IN
cord-009376-a35a92gh	420	4	vaccination	vaccination	NN
cord-009376-a35a92gh	420	5	involve	involve	VB
cord-009376-a35a92gh	420	6	the	the	DT
cord-009376-a35a92gh	420	7	direct	direct	JJ
cord-009376-a35a92gh	420	8	introduction	introduction	NN
cord-009376-a35a92gh	420	9	of	of	IN
cord-009376-a35a92gh	420	10	recombinant	recombinant	JJ
cord-009376-a35a92gh	420	11	plasmid	plasmid	NN
cord-009376-a35a92gh	420	12	DNA	dna	NN
cord-009376-a35a92gh	420	13	Ž	ž	NN
cord-009376-a35a92gh	420	14	gene	gene	NN
cord-009376-a35a92gh	420	15	therapy	therapy	NN
cord-009376-a35a92gh	420	16	vectors	vector	NNS
cord-009376-a35a92gh	420	17	into	into	IN
cord-009376-a35a92gh	420	18	appropriate	appropriate	JJ
cord-009376-a35a92gh	420	19	tissue	tissue	NN
cord-009376-a35a92gh	420	20	Ro-	ro-	XX
cord-009376-a35a92gh	420	21	.	.	.
cord-009376-a35a92gh	421	1	bertson	bertson	NNP
cord-009376-a35a92gh	421	2	and	and	CC
cord-009376-a35a92gh	421	3	Griffiths	Griffiths	NNP
cord-009376-a35a92gh	421	4	,	,	,
cord-009376-a35a92gh	421	5	1998	1998	CD
cord-009376-a35a92gh	421	6	;	;	:
cord-009376-a35a92gh	421	7	Mahato	Mahato	NNP
cord-009376-a35a92gh	421	8	et	et	NNP
cord-009376-a35a92gh	421	9	al	al	NNP
cord-009376-a35a92gh	421	10	.	.	NNP
cord-009376-a35a92gh	421	11	,	,	,
cord-009376-a35a92gh	421	12	1999	1999	CD
cord-009376-a35a92gh	421	13	.	.	.
cord-009376-a35a92gh	422	1	This	this	DT
cord-009376-a35a92gh	422	2	includes	include	VBZ
cord-009376-a35a92gh	422	3	the	the	DT
cord-009376-a35a92gh	422	4	administration	administration	NN
cord-009376-a35a92gh	422	5	of	of	IN
cord-009376-a35a92gh	422	6	plasmid	plasmid	NNP
cord-009376-a35a92gh	422	7	DNA	DNA	NNP
cord-009376-a35a92gh	422	8	complexed	complexe	VBN
cord-009376-a35a92gh	422	9	with	with	IN
cord-009376-a35a92gh	422	10	cationic	cationic	JJ
cord-009376-a35a92gh	422	11	lipids	lipid	NNS
cord-009376-a35a92gh	422	12	for	for	IN
cord-009376-a35a92gh	422	13	the	the	DT
cord-009376-a35a92gh	422	14	treatment	treatment	NN
cord-009376-a35a92gh	422	15	Ž	ž	NN
cord-009376-a35a92gh	422	16	of	of	IN
cord-009376-a35a92gh	422	17	genetic	genetic	JJ
cord-009376-a35a92gh	422	18	and	and	CC
cord-009376-a35a92gh	422	19	acquired	acquire	VBD
cord-009376-a35a92gh	422	20	diseases	disease	NNS
cord-009376-a35a92gh	422	21	Gao	Gao	NNP
cord-009376-a35a92gh	422	22	and	and	CC
cord-009376-a35a92gh	422	23	Haung	Haung	NNP
cord-009376-a35a92gh	422	24	,	,	,
cord-009376-a35a92gh	422	25	.	.	.
cord-009376-a35a92gh	423	1	1995	1995	CD
cord-009376-a35a92gh	423	2	.	.	.
cord-009376-a35a92gh	424	1	Since	since	IN
cord-009376-a35a92gh	424	2	,	,	,
cord-009376-a35a92gh	424	3	plasmids	plasmid	NNS
cord-009376-a35a92gh	424	4	are	be	VBP
cord-009376-a35a92gh	424	5	receiving	receive	VBG
cord-009376-a35a92gh	424	6	increasing	increase	VBG
cord-009376-a35a92gh	424	7	attention	attention	NN
cord-009376-a35a92gh	424	8	for	for	IN
cord-009376-a35a92gh	424	9	use	use	NN
cord-009376-a35a92gh	424	10	as	as	IN
cord-009376-a35a92gh	424	11	vectors	vector	NNS
cord-009376-a35a92gh	424	12	in	in	IN
cord-009376-a35a92gh	424	13	gene	gene	NN
cord-009376-a35a92gh	424	14	therapy	therapy	NN
cord-009376-a35a92gh	424	15	preparations	preparation	NNS
cord-009376-a35a92gh	424	16	,	,	,
cord-009376-a35a92gh	424	17	methods	method	NNS
cord-009376-a35a92gh	424	18	to	to	TO
cord-009376-a35a92gh	424	19	monitor	monitor	VB
cord-009376-a35a92gh	424	20	their	-PRON-	PRP$
cord-009376-a35a92gh	424	21	stability	stability	NN
cord-009376-a35a92gh	424	22	and	and	CC
cord-009376-a35a92gh	424	23	yield	yield	NN
cord-009376-a35a92gh	424	24	is	be	VBZ
cord-009376-a35a92gh	424	25	of	of	IN
cord-009376-a35a92gh	424	26	fundamental	fundamental	JJ
cord-009376-a35a92gh	424	27	importance	importance	NN
cord-009376-a35a92gh	424	28	during	during	IN
cord-009376-a35a92gh	424	29	manufacturing	manufacturing	NN
cord-009376-a35a92gh	424	30	.	.	.
cord-009376-a35a92gh	425	1	Monitoring	monitoring	NN
cord-009376-a35a92gh	425	2	changes	change	NNS
cord-009376-a35a92gh	425	3	in	in	IN
cord-009376-a35a92gh	425	4	transgene	transgene	NN
cord-009376-a35a92gh	425	5	copy	copy	NN
cord-009376-a35a92gh	425	6	number	number	NN
cord-009376-a35a92gh	425	7	and	and	CC
cord-009376-a35a92gh	425	8	levels	level	NNS
cord-009376-a35a92gh	425	9	of	of	IN
cord-009376-a35a92gh	425	10	mRNA	mrna	NN
cord-009376-a35a92gh	425	11	expression	expression	NN
cord-009376-a35a92gh	425	12	is	be	VBZ
cord-009376-a35a92gh	425	13	critical	critical	JJ
cord-009376-a35a92gh	425	14	for	for	IN
cord-009376-a35a92gh	425	15	the	the	DT
cord-009376-a35a92gh	425	16	isolation	isolation	NN
cord-009376-a35a92gh	425	17	of	of	IN
cord-009376-a35a92gh	425	18	stable	stable	JJ
cord-009376-a35a92gh	425	19	cell	cell	NN
cord-009376-a35a92gh	425	20	lines	line	NNS
cord-009376-a35a92gh	425	21	and	and	CC
cord-009376-a35a92gh	425	22	in	in	IN
cord-009376-a35a92gh	425	23	the	the	DT
cord-009376-a35a92gh	425	24	streamlining	streamlining	NN
cord-009376-a35a92gh	425	25	of	of	IN
cord-009376-a35a92gh	425	26	constructs	construct	NNS
cord-009376-a35a92gh	425	27	for	for	IN
cord-009376-a35a92gh	425	28	production	production	NN
cord-009376-a35a92gh	425	29	.	.	.
cord-009376-a35a92gh	426	1	However	however	RB
cord-009376-a35a92gh	426	2	,	,	,
cord-009376-a35a92gh	426	3	production	production	NN
cord-009376-a35a92gh	426	4	and	and	CC
cord-009376-a35a92gh	426	5	purification	purification	NN
cord-009376-a35a92gh	426	6	of	of	IN
cord-009376-a35a92gh	426	7	large	large	JJ
cord-009376-a35a92gh	426	8	amounts	amount	NNS
cord-009376-a35a92gh	426	9	of	of	IN
cord-009376-a35a92gh	426	10	the	the	DT
cord-009376-a35a92gh	426	11	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	426	12	plasmid	plasmid	NN
cord-009376-a35a92gh	426	13	vector	vector	NN
cord-009376-a35a92gh	426	14	can	can	MD
cord-009376-a35a92gh	426	15	be	be	VB
cord-009376-a35a92gh	426	16	hindered	hinder	VBN
cord-009376-a35a92gh	426	17	by	by	IN
cord-009376-a35a92gh	426	18	a	a	DT
cord-009376-a35a92gh	426	19	number	number	NN
cord-009376-a35a92gh	426	20	of	of	IN
cord-009376-a35a92gh	426	21	factors	factor	NNS
cord-009376-a35a92gh	426	22	,	,	,
cord-009376-a35a92gh	426	23	including	include	VBG
cord-009376-a35a92gh	426	24	bacterial	bacterial	JJ
cord-009376-a35a92gh	426	25	host	host	NN
cord-009376-a35a92gh	426	26	strain	strain	NN
cord-009376-a35a92gh	426	27	,	,	,
cord-009376-a35a92gh	426	28	plasmid	plasmid	NN
cord-009376-a35a92gh	426	29	DNA	dna	NN
cord-009376-a35a92gh	426	30	instability	instability	NN
cord-009376-a35a92gh	426	31	and	and	CC
cord-009376-a35a92gh	426	32	copy	copy	NN
cord-009376-a35a92gh	426	33	number	number	NN
cord-009376-a35a92gh	426	34	per	per	IN
cord-009376-a35a92gh	426	35	cell	cell	NN
cord-009376-a35a92gh	426	36	.	.	.
cord-009376-a35a92gh	427	1	Together	together	RB
cord-009376-a35a92gh	427	2	with	with	IN
cord-009376-a35a92gh	427	3	restriction	restriction	NN
cord-009376-a35a92gh	427	4	endonuclease	endonuclease	NN
cord-009376-a35a92gh	427	5	mapping	mapping	NN
cord-009376-a35a92gh	427	6	,	,	,
cord-009376-a35a92gh	427	7	nucleotide	nucleotide	JJ
cord-009376-a35a92gh	427	8	sequence	sequence	NN
cord-009376-a35a92gh	427	9	analysis	analysis	NN
cord-009376-a35a92gh	427	10	and	and	CC
cord-009376-a35a92gh	427	11	phenotypic	phenotypic	JJ
cord-009376-a35a92gh	427	12	testing	testing	NN
cord-009376-a35a92gh	427	13	,	,	,
cord-009376-a35a92gh	427	14	routine	routine	JJ
cord-009376-a35a92gh	427	15	monitoring	monitoring	NN
cord-009376-a35a92gh	427	16	of	of	IN
cord-009376-a35a92gh	427	17	plasmid	plasmid	NN
cord-009376-a35a92gh	427	18	copy	copy	NN
cord-009376-a35a92gh	427	19	number	number	NN
cord-009376-a35a92gh	427	20	is	be	VBZ
cord-009376-a35a92gh	427	21	of	of	IN
cord-009376-a35a92gh	427	22	utmost	utmost	JJ
cord-009376-a35a92gh	427	23	importance	importance	NN
cord-009376-a35a92gh	427	24	in	in	IN
cord-009376-a35a92gh	427	25	large	large	JJ
cord-009376-a35a92gh	427	26	scale	scale	NN
cord-009376-a35a92gh	427	27	cultures	culture	NNS
cord-009376-a35a92gh	427	28	where	where	WRB
cord-009376-a35a92gh	427	29	genetic	genetic	JJ
cord-009376-a35a92gh	427	30	instability	instability	NN
cord-009376-a35a92gh	427	31	can	can	MD
cord-009376-a35a92gh	427	32	Ž	ž	VB
cord-009376-a35a92gh	427	33	.	.	.
cord-009376-a35a92gh	428	1	cause	cause	VB
cord-009376-a35a92gh	428	2	significant	significant	JJ
cord-009376-a35a92gh	428	3	problems	problem	NNS
cord-009376-a35a92gh	429	1	Smith	Smith	NNP
cord-009376-a35a92gh	429	2	et	et	NNP
cord-009376-a35a92gh	429	3	al	al	NNP
cord-009376-a35a92gh	429	4	.	.	NNP
cord-009376-a35a92gh	429	5	,	,	,
cord-009376-a35a92gh	429	6	1996	1996	CD
cord-009376-a35a92gh	429	7	.	.	.
cord-009376-a35a92gh	430	1	Conventional	conventional	JJ
cord-009376-a35a92gh	430	2	procedures	procedure	NNS
cord-009376-a35a92gh	430	3	for	for	IN
cord-009376-a35a92gh	430	4	the	the	DT
cord-009376-a35a92gh	430	5	determination	determination	NN
cord-009376-a35a92gh	430	6	of	of	IN
cord-009376-a35a92gh	430	7	plasmid	plasmid	NN
cord-009376-a35a92gh	430	8	DNA	DNA	NNP
cord-009376-a35a92gh	430	9	copy	copy	NN
cord-009376-a35a92gh	430	10	number	number	NN
cord-009376-a35a92gh	430	11	are	be	VBP
cord-009376-a35a92gh	430	12	laborious	laborious	JJ
cord-009376-a35a92gh	430	13	,	,	,
cord-009376-a35a92gh	430	14	requiring	require	VBG
cord-009376-a35a92gh	430	15	DNA	dna	NN
cord-009376-a35a92gh	430	16	extraction	extraction	NN
cord-009376-a35a92gh	430	17	,	,	,
cord-009376-a35a92gh	430	18	restriction	restriction	NN
cord-009376-a35a92gh	430	19	endonuclease	endonuclease	NN
cord-009376-a35a92gh	430	20	digestion	digestion	NN
cord-009376-a35a92gh	430	21	,	,	,
cord-009376-a35a92gh	430	22	agarose	agarose	VBN
cord-009376-a35a92gh	430	23	electrophoreseis	electrophoreseis	NN
cord-009376-a35a92gh	430	24	and	and	CC
cord-009376-a35a92gh	430	25	ethidium	ethidium	NN
cord-009376-a35a92gh	430	26	bromide	bromide	NN
cord-009376-a35a92gh	430	27	staining	staining	NN
cord-009376-a35a92gh	430	28	.	.	.
cord-009376-a35a92gh	431	1	Therefore	therefore	RB
cord-009376-a35a92gh	431	2	,	,	,
cord-009376-a35a92gh	431	3	any	any	DT
cord-009376-a35a92gh	431	4	high	high	JJ
cord-009376-a35a92gh	431	5	throughput	throughput	NN
cord-009376-a35a92gh	431	6	methods	method	NNS
cord-009376-a35a92gh	431	7	which	which	WDT
cord-009376-a35a92gh	431	8	can	can	MD
cord-009376-a35a92gh	431	9	calculate	calculate	VB
cord-009376-a35a92gh	431	10	the	the	DT
cord-009376-a35a92gh	431	11	copy	copy	NN
cord-009376-a35a92gh	431	12	number	number	NN
cord-009376-a35a92gh	431	13	of	of	IN
cord-009376-a35a92gh	431	14	plasmid	plasmid	NN
cord-009376-a35a92gh	431	15	vector	vector	NN
cord-009376-a35a92gh	431	16	constructs	construct	NNS
cord-009376-a35a92gh	431	17	during	during	IN
cord-009376-a35a92gh	431	18	fermentation	fermentation	NN
cord-009376-a35a92gh	431	19	are	be	VBP
cord-009376-a35a92gh	431	20	paramount	paramount	JJ
cord-009376-a35a92gh	431	21	in	in	IN
cord-009376-a35a92gh	431	22	obtaining	obtain	VBG
cord-009376-a35a92gh	431	23	maximum	maximum	JJ
cord-009376-a35a92gh	431	24	efficiency	efficiency	NN
cord-009376-a35a92gh	431	25	in	in	IN
cord-009376-a35a92gh	431	26	any	any	DT
cord-009376-a35a92gh	431	27	downstream	downstream	JJ
cord-009376-a35a92gh	431	28	process	process	NN
cord-009376-a35a92gh	431	29	.	.	.
cord-009376-a35a92gh	432	1	Using	use	VBG
cord-009376-a35a92gh	432	2	Q	Q	NNP
cord-009376-a35a92gh	432	3	-	-	HYPH
cord-009376-a35a92gh	432	4	PCR	pcr	NN
cord-009376-a35a92gh	432	5	assays	assay	NNS
cord-009376-a35a92gh	432	6	targeted	target	VBN
cord-009376-a35a92gh	432	7	to	to	IN
cord-009376-a35a92gh	432	8	regions	region	NNS
cord-009376-a35a92gh	432	9	such	such	JJ
cord-009376-a35a92gh	432	10	as	as	IN
cord-009376-a35a92gh	432	11	:	:	:
cord-009376-a35a92gh	432	12	the	the	DT
cord-009376-a35a92gh	432	13	plasmid	plasmid	NN
cord-009376-a35a92gh	432	14	origin	origin	NN
cord-009376-a35a92gh	432	15	of	of	IN
cord-009376-a35a92gh	432	16	replication	replication	NN
cord-009376-a35a92gh	432	17	;	;	:
cord-009376-a35a92gh	432	18	the	the	DT
cord-009376-a35a92gh	432	19	antibiotic	antibiotic	JJ
cord-009376-a35a92gh	432	20	resistance	resistance	NN
cord-009376-a35a92gh	432	21	gene	gene	NN
cord-009376-a35a92gh	432	22	;	;	:
cord-009376-a35a92gh	432	23	the	the	DT
cord-009376-a35a92gh	432	24	human	human	JJ
cord-009376-a35a92gh	432	25	CMV	CMV	NNP
cord-009376-a35a92gh	432	26	promoter	promoter	NN
cord-009376-a35a92gh	432	27	;	;	:
cord-009376-a35a92gh	432	28	or	or	CC
cord-009376-a35a92gh	432	29	the	the	DT
cord-009376-a35a92gh	432	30	therapeutic	therapeutic	JJ
cord-009376-a35a92gh	432	31	transgene	transgene	NN
cord-009376-a35a92gh	432	32	,	,	,
cord-009376-a35a92gh	432	33	it	-PRON-	PRP
cord-009376-a35a92gh	432	34	is	be	VBZ
cord-009376-a35a92gh	432	35	possible	possible	JJ
cord-009376-a35a92gh	432	36	to	to	TO
cord-009376-a35a92gh	432	37	identify	identify	VB
cord-009376-a35a92gh	432	38	specific	specific	JJ
cord-009376-a35a92gh	432	39	changes	change	NNS
cord-009376-a35a92gh	432	40	in	in	IN
cord-009376-a35a92gh	432	41	plasmid	plasmid	NN
cord-009376-a35a92gh	433	1	copy	copy	JJ
cord-009376-a35a92gh	433	2	number	number	NN
cord-009376-a35a92gh	433	3	of	of	IN
cord-009376-a35a92gh	433	4	different	different	JJ
cord-009376-a35a92gh	433	5	microbial	microbial	JJ
cord-009376-a35a92gh	433	6	constructs	construct	NNS
cord-009376-a35a92gh	433	7	.	.	.
cord-009376-a35a92gh	434	1	By	by	IN
cord-009376-a35a92gh	434	2	assessing	assess	VBG
cord-009376-a35a92gh	434	3	the	the	DT
cord-009376-a35a92gh	434	4	relationship	relationship	NN
cord-009376-a35a92gh	434	5	between	between	IN
cord-009376-a35a92gh	434	6	cell	cell	NN
cord-009376-a35a92gh	434	7	number	number	NN
cord-009376-a35a92gh	434	8	and	and	CC
cord-009376-a35a92gh	434	9	C	c	NN
cord-009376-a35a92gh	434	10	values	value	NNS
cord-009376-a35a92gh	434	11	,	,	,
cord-009376-a35a92gh	434	12	T	T	NNP
cord-009376-a35a92gh	434	13	linearity	linearity	NN
cord-009376-a35a92gh	434	14	was	be	VBD
cord-009376-a35a92gh	434	15	demonstrated	demonstrate	VBN
cord-009376-a35a92gh	434	16	allowing	allow	VBG
cord-009376-a35a92gh	434	17	the	the	DT
cord-009376-a35a92gh	434	18	direct	direct	JJ
cord-009376-a35a92gh	434	19	determination	determination	NN
cord-009376-a35a92gh	434	20	of	of	IN
cord-009376-a35a92gh	434	21	plasmid	plasmid	NN
cord-009376-a35a92gh	434	22	DNA	DNA	NNP
cord-009376-a35a92gh	434	23	copy	copy	NN
cord-009376-a35a92gh	434	24	number	number	NN
cord-009376-a35a92gh	434	25	from	from	IN
cord-009376-a35a92gh	434	26	culture	culture	NN
cord-009376-a35a92gh	434	27	samples	sample	NNS
cord-009376-a35a92gh	434	28	relative	relative	JJ
cord-009376-a35a92gh	434	29	to	to	IN
cord-009376-a35a92gh	434	30	the	the	DT
cord-009376-a35a92gh	434	31	reference	reference	NN
cord-009376-a35a92gh	434	32	plasmid	plasmid	NN
cord-009376-a35a92gh	434	33	containing	contain	VBG
cord-009376-a35a92gh	434	34	E.	E.	NNP
cord-009376-a35a92gh	434	35	coli	coli	NNS
cord-009376-a35a92gh	434	36	strain	strain	NN
cord-009376-a35a92gh	434	37	.	.	.
cord-009376-a35a92gh	435	1	A	a	DT
cord-009376-a35a92gh	435	2	fourfold	fourfold	JJ
cord-009376-a35a92gh	435	3	difference	difference	NN
cord-009376-a35a92gh	435	4	in	in	IN
cord-009376-a35a92gh	435	5	plasmid	plasmid	NN
cord-009376-a35a92gh	435	6	DNA	DNA	NNP
cord-009376-a35a92gh	435	7	copy	copy	NN
cord-009376-a35a92gh	435	8	number	number	NN
cord-009376-a35a92gh	435	9	was	be	VBD
cord-009376-a35a92gh	435	10	detected	detect	VBN
cord-009376-a35a92gh	435	11	,	,	,
cord-009376-a35a92gh	435	12	therefore	therefore	RB
cord-009376-a35a92gh	435	13	the	the	DT
cord-009376-a35a92gh	435	14	procedure	procedure	NN
cord-009376-a35a92gh	435	15	can	can	MD
cord-009376-a35a92gh	435	16	be	be	VB
cord-009376-a35a92gh	435	17	used	use	VBN
cord-009376-a35a92gh	435	18	to	to	TO
cord-009376-a35a92gh	435	19	monitor	monitor	VB
cord-009376-a35a92gh	435	20	microbial	microbial	JJ
cord-009376-a35a92gh	435	21	fermentation	fermentation	NN
cord-009376-a35a92gh	435	22	or	or	CC
cord-009376-a35a92gh	435	23	optimise	optimise	VB
cord-009376-a35a92gh	435	24	production	production	NN
cord-009376-a35a92gh	435	25	Ž	ž	NN
cord-009376-a35a92gh	435	26	.	.	.
cord-009376-a35a92gh	436	1	methods	method	NNS
cord-009376-a35a92gh	436	2	Tables	Tables	NNP
cord-009376-a35a92gh	436	3	9	9	CD
cord-009376-a35a92gh	436	4	and	and	CC
cord-009376-a35a92gh	436	5	10	10	CD
cord-009376-a35a92gh	436	6	.	.	.
cord-009376-a35a92gh	437	1	The	the	DT
cord-009376-a35a92gh	437	2	two	two	CD
cord-009376-a35a92gh	437	3	methods	method	NNS
cord-009376-a35a92gh	437	4	for	for	IN
cord-009376-a35a92gh	437	5	quantifying	quantify	VBG
cord-009376-a35a92gh	437	6	target	target	NN
cord-009376-a35a92gh	437	7	molecules	molecule	NNS
cord-009376-a35a92gh	437	8	by	by	IN
cord-009376-a35a92gh	437	9	real	real	JJ
cord-009376-a35a92gh	437	10	-	-	HYPH
cord-009376-a35a92gh	437	11	time	time	NN
cord-009376-a35a92gh	437	12	Q	q	NN
cord-009376-a35a92gh	437	13	-	-	HYPH
cord-009376-a35a92gh	437	14	PCR	PCR	NNP
cord-009376-a35a92gh	437	15	are	be	VBP
cord-009376-a35a92gh	437	16	termed	term	VBN
cord-009376-a35a92gh	437	17	relative	relative	JJ
cord-009376-a35a92gh	437	18	and	and	CC
cord-009376-a35a92gh	437	19	absolute	absolute	JJ
cord-009376-a35a92gh	437	20	quantification	quantification	NN
cord-009376-a35a92gh	437	21	.	.	.
cord-009376-a35a92gh	438	1	Relative	relative	JJ
cord-009376-a35a92gh	438	2	quantification	quantification	NN
cord-009376-a35a92gh	438	3	can	can	MD
cord-009376-a35a92gh	438	4	use	use	VB
cord-009376-a35a92gh	438	5	an	an	DT
cord-009376-a35a92gh	438	6	endogenous	endogenous	JJ
cord-009376-a35a92gh	438	7	control	control	NN
cord-009376-a35a92gh	438	8	to	to	TO
cord-009376-a35a92gh	438	9	normalise	normalise	VB
cord-009376-a35a92gh	438	10	the	the	DT
cord-009376-a35a92gh	438	11	amount	amount	NN
cord-009376-a35a92gh	438	12	of	of	IN
cord-009376-a35a92gh	438	13	plasmid	plasmid	NN
cord-009376-a35a92gh	438	14	molecules	molecule	NNS
cord-009376-a35a92gh	438	15	in	in	IN
cord-009376-a35a92gh	438	16	a	a	DT
cord-009376-a35a92gh	438	17	sample	sample	NN
cord-009376-a35a92gh	438	18	.	.	.
cord-009376-a35a92gh	439	1	The	the	DT
cord-009376-a35a92gh	439	2	normalised	normalised	JJ
cord-009376-a35a92gh	439	3	value	value	NN
cord-009376-a35a92gh	439	4	can	can	MD
cord-009376-a35a92gh	439	5	assess	assess	VB
cord-009376-a35a92gh	439	6	significant	significant	JJ
cord-009376-a35a92gh	439	7	increases	increase	NNS
cord-009376-a35a92gh	439	8	or	or	CC
cord-009376-a35a92gh	439	9	decreases	decrease	NNS
cord-009376-a35a92gh	439	10	in	in	IN
cord-009376-a35a92gh	439	11	the	the	DT
cord-009376-a35a92gh	439	12	copy	copy	NN
cord-009376-a35a92gh	439	13	number	number	NN
cord-009376-a35a92gh	439	14	of	of	IN
cord-009376-a35a92gh	439	15	plasmid	plasmid	NN
cord-009376-a35a92gh	439	16	DNA	dna	NN
cord-009376-a35a92gh	439	17	and	and	CC
cord-009376-a35a92gh	439	18	is	be	VBZ
cord-009376-a35a92gh	439	19	independent	independent	JJ
cord-009376-a35a92gh	439	20	of	of	IN
cord-009376-a35a92gh	439	21	conventional	conventional	JJ
cord-009376-a35a92gh	439	22	measurement	measurement	NN
cord-009376-a35a92gh	439	23	of	of	IN
cord-009376-a35a92gh	439	24	cell	cell	NN
cord-009376-a35a92gh	439	25	number	number	NN
cord-009376-a35a92gh	439	26	.	.	.
cord-009376-a35a92gh	440	1	The	the	DT
cord-009376-a35a92gh	440	2	incorporation	incorporation	NN
cord-009376-a35a92gh	440	3	of	of	IN
cord-009376-a35a92gh	440	4	relative	relative	JJ
cord-009376-a35a92gh	440	5	quantification	quantification	NN
cord-009376-a35a92gh	440	6	could	could	MD
cord-009376-a35a92gh	440	7	provide	provide	VB
cord-009376-a35a92gh	440	8	the	the	DT
cord-009376-a35a92gh	440	9	potential	potential	NN
cord-009376-a35a92gh	440	10	to	to	IN
cord-009376-a35a92gh	440	11	screen	screen	NN
cord-009376-a35a92gh	440	12	colonies	colony	NNS
cord-009376-a35a92gh	440	13	or	or	CC
cord-009376-a35a92gh	440	14	volumes	volume	NNS
cord-009376-a35a92gh	440	15	of	of	IN
cord-009376-a35a92gh	440	16	culture	culture	NN
cord-009376-a35a92gh	440	17	di-	di-	XX
cord-009376-a35a92gh	441	1	A	a	DT
cord-009376-a35a92gh	441	2	fourfold	fourfold	JJ
cord-009376-a35a92gh	441	3	difference	difference	NN
cord-009376-a35a92gh	441	4	in	in	IN
cord-009376-a35a92gh	441	5	cell	cell	NN
cord-009376-a35a92gh	441	6	number	number	NN
cord-009376-a35a92gh	441	7	was	be	VBD
cord-009376-a35a92gh	441	8	detected	detect	VBN
cord-009376-a35a92gh	441	9	.	.	.
cord-009376-a35a92gh	442	1	Confidence	confidence	NN
cord-009376-a35a92gh	442	2	interval	interval	NN
cord-009376-a35a92gh	442	3	data	datum	NNS
cord-009376-a35a92gh	442	4	show	show	VBP
cord-009376-a35a92gh	442	5	statistical	statistical	JJ
cord-009376-a35a92gh	442	6	differences	difference	NNS
cord-009376-a35a92gh	442	7	.	.	.
cord-009376-a35a92gh	443	1	(	(	-LRB-
cord-009376-a35a92gh	443	2	)	)	-RRB-
cord-009376-a35a92gh	443	3	rectly	rectly	RB
cord-009376-a35a92gh	443	4	.	.	.
cord-009376-a35a92gh	444	1	The	the	DT
cord-009376-a35a92gh	444	2	relative	relative	JJ
cord-009376-a35a92gh	444	3	quantification	quantification	NN
cord-009376-a35a92gh	444	4	will	will	MD
cord-009376-a35a92gh	444	5	calculate	calculate	VB
cord-009376-a35a92gh	444	6	numerical	numerical	JJ
cord-009376-a35a92gh	444	7	difference	difference	NN
cord-009376-a35a92gh	444	8	between	between	IN
cord-009376-a35a92gh	444	9	the	the	DT
cord-009376-a35a92gh	444	10	endogenous	endogenous	JJ
cord-009376-a35a92gh	444	11	chromosomal	chromosomal	JJ
cord-009376-a35a92gh	444	12	gene	gene	NN
cord-009376-a35a92gh	444	13	and	and	CC
cord-009376-a35a92gh	444	14	the	the	DT
cord-009376-a35a92gh	444	15	number	number	NN
cord-009376-a35a92gh	444	16	of	of	IN
cord-009376-a35a92gh	444	17	plasmid	plasmid	NN
cord-009376-a35a92gh	444	18	molecules	molecule	NNS
cord-009376-a35a92gh	444	19	,	,	,
cord-009376-a35a92gh	444	20	in	in	IN
cord-009376-a35a92gh	444	21	a	a	DT
cord-009376-a35a92gh	444	22	microbial	microbial	JJ
cord-009376-a35a92gh	444	23	sample	sample	NN
cord-009376-a35a92gh	444	24	.	.	.
cord-009376-a35a92gh	445	1	In	in	IN
cord-009376-a35a92gh	445	2	this	this	DT
cord-009376-a35a92gh	445	3	way	way	NN
cord-009376-a35a92gh	445	4	,	,	,
cord-009376-a35a92gh	445	5	significant	significant	JJ
cord-009376-a35a92gh	445	6	differences	difference	NNS
cord-009376-a35a92gh	445	7	between	between	IN
cord-009376-a35a92gh	445	8	plasmid	plasmid	NN
cord-009376-a35a92gh	445	9	target	target	NN
cord-009376-a35a92gh	445	10	and	and	CC
cord-009376-a35a92gh	445	11	endogenous	endogenous	JJ
cord-009376-a35a92gh	445	12	control	control	NN
cord-009376-a35a92gh	445	13	,	,	,
cord-009376-a35a92gh	445	14	are	be	VBP
cord-009376-a35a92gh	445	15	detected	detect	VBN
cord-009376-a35a92gh	445	16	to	to	TO
cord-009376-a35a92gh	445	17	provide	provide	VB
cord-009376-a35a92gh	445	18	information	information	NN
cord-009376-a35a92gh	445	19	on	on	IN
cord-009376-a35a92gh	445	20	plasmid	plasmid	NN
cord-009376-a35a92gh	445	21	DNA	dna	NN
cord-009376-a35a92gh	445	22	molecules	molecule	NNS
cord-009376-a35a92gh	445	23	per	per	IN
cord-009376-a35a92gh	445	24	cell	cell	NN
cord-009376-a35a92gh	445	25	.	.	.
cord-009376-a35a92gh	446	1	Using	use	VBG
cord-009376-a35a92gh	446	2	this	this	DT
cord-009376-a35a92gh	446	3	approach	approach	NN
cord-009376-a35a92gh	446	4	,	,	,
cord-009376-a35a92gh	446	5	the	the	DT
cord-009376-a35a92gh	446	6	assay	assay	NN
cord-009376-a35a92gh	446	7	could	could	MD
cord-009376-a35a92gh	446	8	be	be	VB
cord-009376-a35a92gh	446	9	independent	independent	JJ
cord-009376-a35a92gh	446	10	of	of	IN
cord-009376-a35a92gh	446	11	OD	OD	NNP
cord-009376-a35a92gh	446	12	standardisation	standardisation	NN
cord-009376-a35a92gh	446	13	and	and	CC
cord-009376-a35a92gh	446	14	still	still	RB
cord-009376-a35a92gh	446	15	be	be	VB
cord-009376-a35a92gh	446	16	able	able	JJ
cord-009376-a35a92gh	446	17	to	to	IN
cord-009376-a35a92gh	446	18	600	600	CD
cord-009376-a35a92gh	446	19	detect	detect	VB
cord-009376-a35a92gh	446	20	a	a	DT
cord-009376-a35a92gh	446	21	two	two	CD
cord-009376-a35a92gh	446	22	-	-	HYPH
cord-009376-a35a92gh	446	23	to	to	IN
cord-009376-a35a92gh	446	24	fourfold	fourfold	JJ
cord-009376-a35a92gh	446	25	difference	difference	NN
cord-009376-a35a92gh	446	26	in	in	IN
cord-009376-a35a92gh	446	27	copy	copy	NN
cord-009376-a35a92gh	446	28	number	number	NN
cord-009376-a35a92gh	446	29	.	.	.
cord-009376-a35a92gh	447	1	Taken	take	VBN
cord-009376-a35a92gh	447	2	together	together	RB
cord-009376-a35a92gh	447	3	,	,	,
cord-009376-a35a92gh	447	4	Q	Q	NNP
cord-009376-a35a92gh	447	5	-	-	HYPH
cord-009376-a35a92gh	447	6	PCR	PCR	NNP
cord-009376-a35a92gh	447	7	has	have	VBZ
cord-009376-a35a92gh	447	8	severe	severe	JJ
cord-009376-a35a92gh	447	9	implications	implication	NNS
cord-009376-a35a92gh	447	10	in	in	IN
cord-009376-a35a92gh	447	11	monitoring	monitor	VBG
cord-009376-a35a92gh	447	12	the	the	DT
cord-009376-a35a92gh	447	13	genetic	genetic	JJ
cord-009376-a35a92gh	447	14	stability	stability	NN
cord-009376-a35a92gh	447	15	of	of	IN
cord-009376-a35a92gh	447	16	plasmid	plasmid	NN
cord-009376-a35a92gh	447	17	DNA	dna	NN
cord-009376-a35a92gh	447	18	in	in	IN
cord-009376-a35a92gh	447	19	microbial	microbial	JJ
cord-009376-a35a92gh	447	20	constructs	construct	NNS
cord-009376-a35a92gh	447	21	selected	select	VBN
cord-009376-a35a92gh	447	22	for	for	IN
cord-009376-a35a92gh	447	23	production	production	NN
cord-009376-a35a92gh	447	24	.	.	.
cord-009376-a35a92gh	448	1	Q	Q	NNP
cord-009376-a35a92gh	448	2	-	-	HYPH
cord-009376-a35a92gh	448	3	PCR	PCR	NNP
cord-009376-a35a92gh	448	4	can	can	MD
cord-009376-a35a92gh	448	5	be	be	VB
cord-009376-a35a92gh	448	6	used	use	VBN
cord-009376-a35a92gh	448	7	to	to	TO
cord-009376-a35a92gh	448	8	measure	measure	VB
cord-009376-a35a92gh	448	9	the	the	DT
cord-009376-a35a92gh	448	10	copy	copy	NN
cord-009376-a35a92gh	448	11	number	number	NN
cord-009376-a35a92gh	448	12	over	over	IN
cord-009376-a35a92gh	448	13	a	a	DT
cord-009376-a35a92gh	448	14	wide	wide	JJ
cord-009376-a35a92gh	448	15	range	range	NN
cord-009376-a35a92gh	448	16	of	of	IN
cord-009376-a35a92gh	448	17	different	different	JJ
cord-009376-a35a92gh	448	18	cell	cell	NN
cord-009376-a35a92gh	448	19	numbers	number	NNS
cord-009376-a35a92gh	448	20	or	or	CC
cord-009376-a35a92gh	448	21	genome	genome	NN
cord-009376-a35a92gh	448	22	equivalents	equivalent	NNS
cord-009376-a35a92gh	448	23	,	,	,
cord-009376-a35a92gh	448	24	and	and	CC
cord-009376-a35a92gh	448	25	is	be	VBZ
cord-009376-a35a92gh	448	26	able	able	JJ
cord-009376-a35a92gh	448	27	to	to	TO
cord-009376-a35a92gh	448	28	assess	assess	VB
cord-009376-a35a92gh	448	29	copy	copy	NN
cord-009376-a35a92gh	448	30	Ž	ž	NN
cord-009376-a35a92gh	448	31	.	.	.
cord-009376-a35a92gh	449	1	number	number	NN
cord-009376-a35a92gh	449	2	stability	stability	NN
cord-009376-a35a92gh	449	3	in	in	IN
cord-009376-a35a92gh	449	4	master	master	NN
cord-009376-a35a92gh	449	5	cell	cell	NN
cord-009376-a35a92gh	449	6	bank	bank	NN
cord-009376-a35a92gh	449	7	MCB	MCB	NNP
cord-009376-a35a92gh	449	8	,	,	,
cord-009376-a35a92gh	449	9	Ž	Ž	NNP
cord-009376-a35a92gh	449	10	.	.	.
cord-009376-a35a92gh	450	1	working	work	VBG
cord-009376-a35a92gh	450	2	cell	cell	NN
cord-009376-a35a92gh	450	3	bank	bank	NN
cord-009376-a35a92gh	450	4	WCB	WCB	NNP
cord-009376-a35a92gh	450	5	and	and	CC
cord-009376-a35a92gh	450	6	post	post	NN
cord-009376-a35a92gh	450	7	production	production	NN
cord-009376-a35a92gh	450	8	Ž	ž	NN
cord-009376-a35a92gh	450	9	.	.	.
cord-009376-a35a92gh	451	1	cell	cell	NN
cord-009376-a35a92gh	451	2	bank	bank	NNP
cord-009376-a35a92gh	451	3	PCB	PCB	NNP
cord-009376-a35a92gh	451	4	.	.	.
cord-009376-a35a92gh	452	1	Significant	significant	JJ
cord-009376-a35a92gh	452	2	changes	change	NNS
cord-009376-a35a92gh	452	3	are	be	VBP
cord-009376-a35a92gh	452	4	demonstrated	demonstrate	VBN
cord-009376-a35a92gh	452	5	by	by	IN
cord-009376-a35a92gh	452	6	performing	perform	VBG
cord-009376-a35a92gh	452	7	statistical	statistical	JJ
cord-009376-a35a92gh	452	8	analysis	analysis	NN
cord-009376-a35a92gh	452	9	on	on	IN
cord-009376-a35a92gh	452	10	the	the	DT
cord-009376-a35a92gh	452	11	C	C	NNP
cord-009376-a35a92gh	452	12	value	value	NN
cord-009376-a35a92gh	452	13	obtained	obtain	VBN
cord-009376-a35a92gh	452	14	from	from	IN
cord-009376-a35a92gh	452	15	the	the	DT
cord-009376-a35a92gh	452	16	MCB	MCB	NNP
cord-009376-a35a92gh	452	17	,	,	,
cord-009376-a35a92gh	452	18	WCB	WCB	NNP
cord-009376-a35a92gh	452	19	and	and	CC
cord-009376-a35a92gh	452	20	T	T	NNP
cord-009376-a35a92gh	452	21	PCB	PCB	NNP
cord-009376-a35a92gh	452	22	.	.	.
cord-009376-a35a92gh	453	1	The	the	DT
cord-009376-a35a92gh	453	2	copy	copy	NN
cord-009376-a35a92gh	453	3	number	number	NN
cord-009376-a35a92gh	453	4	for	for	IN
cord-009376-a35a92gh	453	5	each	each	DT
cord-009376-a35a92gh	453	6	cell	cell	NN
cord-009376-a35a92gh	453	7	bank	bank	NN
cord-009376-a35a92gh	453	8	is	be	VBZ
cord-009376-a35a92gh	453	9	expressed	express	VBN
cord-009376-a35a92gh	453	10	in	in	IN
cord-009376-a35a92gh	453	11	a	a	DT
cord-009376-a35a92gh	453	12	confidence	confidence	NN
cord-009376-a35a92gh	453	13	interval	interval	NN
cord-009376-a35a92gh	453	14	and	and	CC
cord-009376-a35a92gh	453	15	if	if	IN
cord-009376-a35a92gh	453	16	the	the	DT
cord-009376-a35a92gh	453	17	numerical	numerical	JJ
cord-009376-a35a92gh	453	18	C	c	NN
cord-009376-a35a92gh	453	19	values	value	NNS
cord-009376-a35a92gh	453	20	of	of	IN
cord-009376-a35a92gh	453	21	the	the	DT
cord-009376-a35a92gh	453	22	confidence	confidence	NN
cord-009376-a35a92gh	453	23	intervals	interval	NNS
cord-009376-a35a92gh	453	24	T	T	NNP
cord-009376-a35a92gh	453	25	from	from	IN
cord-009376-a35a92gh	453	26	each	each	DT
cord-009376-a35a92gh	453	27	cell	cell	NN
cord-009376-a35a92gh	453	28	bank	bank	NN
cord-009376-a35a92gh	453	29	overlap	overlap	NN
cord-009376-a35a92gh	453	30	,	,	,
cord-009376-a35a92gh	453	31	this	this	DT
cord-009376-a35a92gh	453	32	demonstrates	demonstrate	VBZ
cord-009376-a35a92gh	453	33	that	that	IN
cord-009376-a35a92gh	453	34	the	the	DT
cord-009376-a35a92gh	453	35	data	datum	NNS
cord-009376-a35a92gh	453	36	derived	derive	VBN
cord-009376-a35a92gh	453	37	from	from	IN
cord-009376-a35a92gh	453	38	each	each	DT
cord-009376-a35a92gh	453	39	stage	stage	NN
cord-009376-a35a92gh	453	40	of	of	IN
cord-009376-a35a92gh	453	41	the	the	DT
cord-009376-a35a92gh	453	42	manufacturing	manufacturing	NN
cord-009376-a35a92gh	453	43	process	process	NN
cord-009376-a35a92gh	453	44	are	be	VBP
cord-009376-a35a92gh	453	45	derived	derive	VBN
cord-009376-a35a92gh	453	46	from	from	IN
cord-009376-a35a92gh	453	47	the	the	DT
cord-009376-a35a92gh	453	48	same	same	JJ
cord-009376-a35a92gh	453	49	statistical	statistical	JJ
cord-009376-a35a92gh	453	50	populations	population	NNS
cord-009376-a35a92gh	453	51	.	.	.
cord-009376-a35a92gh	454	1	The	the	DT
cord-009376-a35a92gh	454	2	statistical	statistical	JJ
cord-009376-a35a92gh	454	3	data	datum	NNS
cord-009376-a35a92gh	454	4	at	at	IN
cord-009376-a35a92gh	454	5	the	the	DT
cord-009376-a35a92gh	454	6	Ps	Ps	NNP
cord-009376-a35a92gh	454	7	0.05	0.05	CD
cord-009376-a35a92gh	454	8	level	level	NN
cord-009376-a35a92gh	454	9	,	,	,
cord-009376-a35a92gh	454	10	provides	provide	VBZ
cord-009376-a35a92gh	454	11	a	a	DT
cord-009376-a35a92gh	454	12	means	means	NN
cord-009376-a35a92gh	454	13	of	of	IN
cord-009376-a35a92gh	454	14	monitoring	monitor	VBG
cord-009376-a35a92gh	454	15	significant	significant	JJ
cord-009376-a35a92gh	454	16	changes	change	NNS
cord-009376-a35a92gh	454	17	in	in	IN
cord-009376-a35a92gh	454	18	the	the	DT
cord-009376-a35a92gh	454	19	copy	copy	NN
cord-009376-a35a92gh	454	20	number	number	NN
cord-009376-a35a92gh	454	21	during	during	IN
cord-009376-a35a92gh	454	22	the	the	DT
cord-009376-a35a92gh	454	23	manufacturing	manufacturing	NN
cord-009376-a35a92gh	454	24	process	process	NN
cord-009376-a35a92gh	454	25	.	.	.
cord-009376-a35a92gh	455	1	Q	Q	NNP
cord-009376-a35a92gh	455	2	-	-	HYPH
cord-009376-a35a92gh	455	3	PCR	PCR	NNP
cord-009376-a35a92gh	455	4	is	be	VBZ
cord-009376-a35a92gh	455	5	more	more	RBR
cord-009376-a35a92gh	455	6	accurate	accurate	JJ
cord-009376-a35a92gh	455	7	than	than	IN
cord-009376-a35a92gh	455	8	the	the	DT
cord-009376-a35a92gh	455	9	Southern	southern	JJ
cord-009376-a35a92gh	455	10	blot	blot	NN
cord-009376-a35a92gh	455	11	technique	technique	NN
cord-009376-a35a92gh	455	12	for	for	IN
cord-009376-a35a92gh	455	13	the	the	DT
cord-009376-a35a92gh	455	14	detection	detection	NN
cord-009376-a35a92gh	455	15	of	of	IN
cord-009376-a35a92gh	455	16	alterations	alteration	NNS
cord-009376-a35a92gh	455	17	in	in	IN
cord-009376-a35a92gh	455	18	the	the	DT
cord-009376-a35a92gh	455	19	gene	gene	NN
cord-009376-a35a92gh	455	20	copy	copy	NN
cord-009376-a35a92gh	455	21	number	number	NN
cord-009376-a35a92gh	455	22	,	,	,
cord-009376-a35a92gh	455	23	since	since	IN
cord-009376-a35a92gh	455	24	estimation	estimation	NN
cord-009376-a35a92gh	455	25	by	by	IN
cord-009376-a35a92gh	455	26	Southern	southern	JJ
cord-009376-a35a92gh	455	27	blotting	blotting	NN
cord-009376-a35a92gh	455	28	relies	rely	VBZ
cord-009376-a35a92gh	455	29	on	on	IN
cord-009376-a35a92gh	455	30	comparison	comparison	NN
cord-009376-a35a92gh	455	31	of	of	IN
cord-009376-a35a92gh	455	32	the	the	DT
cord-009376-a35a92gh	455	33	banding	banding	NN
cord-009376-a35a92gh	455	34	intensity	intensity	NN
cord-009376-a35a92gh	455	35	on	on	IN
cord-009376-a35a92gh	455	36	an	an	DT
cord-009376-a35a92gh	455	37	autoradiograph	autoradiograph	NN
cord-009376-a35a92gh	455	38	.	.	.
cord-009376-a35a92gh	456	1	The	the	DT
cord-009376-a35a92gh	456	2	Southern	southern	JJ
cord-009376-a35a92gh	456	3	blot	blot	NN
cord-009376-a35a92gh	456	4	analysis	analysis	NN
cord-009376-a35a92gh	456	5	generates	generate	VBZ
cord-009376-a35a92gh	456	6	useful	useful	JJ
cord-009376-a35a92gh	456	7	information	information	NN
cord-009376-a35a92gh	456	8	about	about	IN
cord-009376-a35a92gh	456	9	the	the	DT
cord-009376-a35a92gh	456	10	structural	structural	JJ
cord-009376-a35a92gh	456	11	integrity	integrity	NN
cord-009376-a35a92gh	456	12	of	of	IN
cord-009376-a35a92gh	456	13	the	the	DT
cord-009376-a35a92gh	456	14	integrated	integrate	VBN
cord-009376-a35a92gh	456	15	construct	construct	NN
cord-009376-a35a92gh	456	16	but	but	CC
cord-009376-a35a92gh	456	17	provides	provide	VBZ
cord-009376-a35a92gh	456	18	only	only	RB
cord-009376-a35a92gh	456	19	an	an	DT
cord-009376-a35a92gh	456	20	approximate	approximate	JJ
cord-009376-a35a92gh	456	21	evaluation	evaluation	NN
cord-009376-a35a92gh	456	22	of	of	IN
cord-009376-a35a92gh	456	23	the	the	DT
cord-009376-a35a92gh	456	24	copy	copy	NN
cord-009376-a35a92gh	456	25	number	number	NN
cord-009376-a35a92gh	456	26	.	.	.
cord-009376-a35a92gh	457	1	In	in	IN
cord-009376-a35a92gh	457	2	terms	term	NNS
cord-009376-a35a92gh	457	3	of	of	IN
cord-009376-a35a92gh	457	4	stability	stability	NN
cord-009376-a35a92gh	457	5	,	,	,
cord-009376-a35a92gh	457	6	an	an	DT
cord-009376-a35a92gh	457	7	increase	increase	NN
cord-009376-a35a92gh	457	8	in	in	IN
cord-009376-a35a92gh	457	9	copy	copy	NN
cord-009376-a35a92gh	457	10	number	number	NN
cord-009376-a35a92gh	457	11	of	of	IN
cord-009376-a35a92gh	457	12	the	the	DT
cord-009376-a35a92gh	457	13	transgene	transgene	NN
cord-009376-a35a92gh	457	14	in	in	IN
cord-009376-a35a92gh	457	15	the	the	DT
cord-009376-a35a92gh	457	16	cell	cell	NN
cord-009376-a35a92gh	457	17	line	line	NN
cord-009376-a35a92gh	457	18	can	can	MD
cord-009376-a35a92gh	457	19	occur	occur	VB
cord-009376-a35a92gh	457	20	by	by	IN
cord-009376-a35a92gh	457	21	chromosomal	chromosomal	JJ
cord-009376-a35a92gh	457	22	duplication	duplication	NN
cord-009376-a35a92gh	457	23	.	.	.
cord-009376-a35a92gh	458	1	If	if	IN
cord-009376-a35a92gh	458	2	significant	significant	JJ
cord-009376-a35a92gh	458	3	increases	increase	NNS
cord-009376-a35a92gh	458	4	in	in	IN
cord-009376-a35a92gh	458	5	copy	copy	NN
cord-009376-a35a92gh	458	6	number	number	NN
cord-009376-a35a92gh	458	7	are	be	VBP
cord-009376-a35a92gh	458	8	detected	detect	VBN
cord-009376-a35a92gh	458	9	in	in	IN
cord-009376-a35a92gh	458	10	the	the	DT
cord-009376-a35a92gh	458	11	manufacturing	manufacturing	NN
cord-009376-a35a92gh	458	12	process	process	NN
cord-009376-a35a92gh	458	13	,	,	,
cord-009376-a35a92gh	458	14	Fluorescent	Fluorescent	NNP
cord-009376-a35a92gh	458	15	In	in	IN
cord-009376-a35a92gh	458	16	Situ	Situ	NNP
cord-009376-a35a92gh	458	17	Ž	ž	NN
cord-009376-a35a92gh	458	18	.	.	.
cord-009376-a35a92gh	459	1	Hybridisation	Hybridisation	NNP
cord-009376-a35a92gh	459	2	FISH	fish	NN
cord-009376-a35a92gh	459	3	analysis	analysis	NN
cord-009376-a35a92gh	459	4	can	can	MD
cord-009376-a35a92gh	459	5	be	be	VB
cord-009376-a35a92gh	459	6	performed	perform	VBN
cord-009376-a35a92gh	459	7	to	to	TO
cord-009376-a35a92gh	459	8	investigate	investigate	VB
cord-009376-a35a92gh	459	9	further	further	RB
cord-009376-a35a92gh	459	10	the	the	DT
cord-009376-a35a92gh	459	11	stability	stability	NN
cord-009376-a35a92gh	459	12	of	of	IN
cord-009376-a35a92gh	459	13	the	the	DT
cord-009376-a35a92gh	459	14	construct	construct	NN
cord-009376-a35a92gh	459	15	.	.	.
cord-009376-a35a92gh	460	1	Direct	direct	JJ
cord-009376-a35a92gh	460	2	chromosomal	chromosomal	JJ
cord-009376-a35a92gh	460	3	duplication	duplication	NN
cord-009376-a35a92gh	460	4	,	,	,
cord-009376-a35a92gh	460	5	with	with	IN
cord-009376-a35a92gh	460	6	no	no	DT
cord-009376-a35a92gh	460	7	associated	associate	VBN
cord-009376-a35a92gh	460	8	structural	structural	JJ
cord-009376-a35a92gh	460	9	alterations	alteration	NNS
cord-009376-a35a92gh	460	10	,	,	,
cord-009376-a35a92gh	460	11	would	would	MD
cord-009376-a35a92gh	460	12	be	be	VB
cord-009376-a35a92gh	460	13	missed	miss	VBN
cord-009376-a35a92gh	460	14	by	by	IN
cord-009376-a35a92gh	460	15	southern	southern	JJ
cord-009376-a35a92gh	460	16	analysis	analysis	NN
cord-009376-a35a92gh	460	17	,	,	,
cord-009376-a35a92gh	460	18	if	if	IN
cord-009376-a35a92gh	460	19	the	the	DT
cord-009376-a35a92gh	460	20	integrated	integrated	JJ
cord-009376-a35a92gh	460	21	expression	expression	NN
cord-009376-a35a92gh	460	22	cassette	cassette	NN
cord-009376-a35a92gh	460	23	remains	remain	VBZ
cord-009376-a35a92gh	460	24	unaltered	unaltered	JJ
cord-009376-a35a92gh	460	25	when	when	WRB
cord-009376-a35a92gh	460	26	duplicated	duplicate	VBN
cord-009376-a35a92gh	460	27	.	.	.
cord-009376-a35a92gh	461	1	Structural	structural	JJ
cord-009376-a35a92gh	461	2	analysis	analysis	NN
cord-009376-a35a92gh	461	3	will	will	MD
cord-009376-a35a92gh	461	4	only	only	RB
cord-009376-a35a92gh	461	5	detect	detect	VB
cord-009376-a35a92gh	461	6	changes	change	NNS
cord-009376-a35a92gh	461	7	in	in	IN
cord-009376-a35a92gh	461	8	the	the	DT
cord-009376-a35a92gh	461	9	expression	expression	NN
cord-009376-a35a92gh	461	10	cassette	cassette	NN
cord-009376-a35a92gh	461	11	,	,	,
cord-009376-a35a92gh	461	12	such	such	JJ
cord-009376-a35a92gh	461	13	as	as	IN
cord-009376-a35a92gh	461	14	those	those	DT
cord-009376-a35a92gh	461	15	caused	cause	VBN
cord-009376-a35a92gh	461	16	by	by	IN
cord-009376-a35a92gh	461	17	deletions	deletion	NNS
cord-009376-a35a92gh	461	18	,	,	,
cord-009376-a35a92gh	461	19	insertions	insertion	NNS
cord-009376-a35a92gh	461	20	and	and	CC
cord-009376-a35a92gh	461	21	recombinationrduplication	recombinationrduplication	NN
cord-009376-a35a92gh	461	22	of	of	IN
cord-009376-a35a92gh	461	23	the	the	DT
cord-009376-a35a92gh	461	24	integrated	integrate	VBN
cord-009376-a35a92gh	461	25	construct	construct	NN
cord-009376-a35a92gh	461	26	to	to	IN
cord-009376-a35a92gh	461	27	another	another	DT
cord-009376-a35a92gh	461	28	chromosomal	chromosomal	JJ
cord-009376-a35a92gh	461	29	location	location	NN
cord-009376-a35a92gh	461	30	.	.	.
cord-009376-a35a92gh	462	1	Therefore	therefore	RB
cord-009376-a35a92gh	462	2	,	,	,
cord-009376-a35a92gh	462	3	rapid	rapid	JJ
cord-009376-a35a92gh	462	4	screening	screening	NN
cord-009376-a35a92gh	462	5	with	with	IN
cord-009376-a35a92gh	462	6	Q	Q	NNP
cord-009376-a35a92gh	462	7	-	-	HYPH
cord-009376-a35a92gh	462	8	PCR	PCR	NNP
cord-009376-a35a92gh	462	9	assays	assay	NNS
cord-009376-a35a92gh	462	10	will	will	MD
cord-009376-a35a92gh	462	11	aid	aid	VB
cord-009376-a35a92gh	462	12	in	in	IN
cord-009376-a35a92gh	462	13	the	the	DT
cord-009376-a35a92gh	462	14	detection	detection	NN
cord-009376-a35a92gh	462	15	of	of	IN
cord-009376-a35a92gh	462	16	any	any	DT
cord-009376-a35a92gh	462	17	chromosomal	chromosomal	JJ
cord-009376-a35a92gh	462	18	duplication	duplication	NN
cord-009376-a35a92gh	462	19	,	,	,
cord-009376-a35a92gh	462	20	which	which	WDT
cord-009376-a35a92gh	462	21	results	result	VBZ
cord-009376-a35a92gh	462	22	in	in	IN
cord-009376-a35a92gh	462	23	an	an	DT
cord-009376-a35a92gh	462	24	increase	increase	NN
cord-009376-a35a92gh	462	25	in	in	IN
cord-009376-a35a92gh	462	26	copy	copy	NN
cord-009376-a35a92gh	462	27	number	number	NN
cord-009376-a35a92gh	462	28	but	but	CC
cord-009376-a35a92gh	462	29	no	no	DT
cord-009376-a35a92gh	462	30	associated	associate	VBN
cord-009376-a35a92gh	462	31	structural	structural	JJ
cord-009376-a35a92gh	462	32	alterations	alteration	NNS
cord-009376-a35a92gh	462	33	detectable	detectable	JJ
cord-009376-a35a92gh	462	34	by	by	IN
cord-009376-a35a92gh	462	35	Southern	southern	JJ
cord-009376-a35a92gh	462	36	blot	blot	NN
cord-009376-a35a92gh	462	37	analysis	analysis	NN
cord-009376-a35a92gh	462	38	.	.	.
cord-009376-a35a92gh	463	1	Because	because	IN
cord-009376-a35a92gh	463	2	of	of	IN
cord-009376-a35a92gh	463	3	the	the	DT
cord-009376-a35a92gh	463	4	increasing	increase	VBG
cord-009376-a35a92gh	463	5	rate	rate	NN
cord-009376-a35a92gh	463	6	of	of	IN
cord-009376-a35a92gh	463	7	discovery	discovery	NN
cord-009376-a35a92gh	463	8	of	of	IN
cord-009376-a35a92gh	463	9	previously	previously	RB
cord-009376-a35a92gh	463	10	unrecognised	unrecognised	JJ
cord-009376-a35a92gh	463	11	viruses	virus	NNS
cord-009376-a35a92gh	463	12	,	,	,
cord-009376-a35a92gh	463	13	safety	safety	NN
cord-009376-a35a92gh	463	14	is	be	VBZ
cord-009376-a35a92gh	463	15	becoming	become	VBG
cord-009376-a35a92gh	463	16	of	of	IN
cord-009376-a35a92gh	463	17	the	the	DT
cord-009376-a35a92gh	463	18	utmost	utmost	JJ
cord-009376-a35a92gh	463	19	concern	concern	NN
cord-009376-a35a92gh	463	20	in	in	IN
cord-009376-a35a92gh	463	21	biotechnology	biotechnology	NN
cord-009376-a35a92gh	463	22	manufacturing	manufacturing	NN
cord-009376-a35a92gh	463	23	.	.	.
cord-009376-a35a92gh	464	1	In	in	IN
cord-009376-a35a92gh	464	2	addition	addition	NN
cord-009376-a35a92gh	464	3	,	,	,
cord-009376-a35a92gh	464	4	there	there	EX
cord-009376-a35a92gh	464	5	is	be	VBZ
cord-009376-a35a92gh	464	6	additional	additional	JJ
cord-009376-a35a92gh	464	7	pressure	pressure	NN
cord-009376-a35a92gh	464	8	for	for	IN
cord-009376-a35a92gh	464	9	fast	fast	JJ
cord-009376-a35a92gh	464	10	assay	assay	NN
cord-009376-a35a92gh	464	11	development	development	NN
cord-009376-a35a92gh	464	12	,	,	,
cord-009376-a35a92gh	464	13	optimisation	optimisation	NN
cord-009376-a35a92gh	464	14	and	and	CC
cord-009376-a35a92gh	464	15	validation	validation	NN
cord-009376-a35a92gh	464	16	of	of	IN
cord-009376-a35a92gh	464	17	testing	testing	NN
cord-009376-a35a92gh	464	18	methods	method	NNS
cord-009376-a35a92gh	464	19	.	.	.
cord-009376-a35a92gh	465	1	Now	now	RB
cord-009376-a35a92gh	465	2	that	that	DT
cord-009376-a35a92gh	465	3	validated	validate	VBN
cord-009376-a35a92gh	465	4	tests	test	NNS
cord-009376-a35a92gh	465	5	are	be	VBP
cord-009376-a35a92gh	465	6	available	available	JJ
cord-009376-a35a92gh	465	7	,	,	,
cord-009376-a35a92gh	465	8	Q	Q	NNP
cord-009376-a35a92gh	465	9	-	-	HYPH
cord-009376-a35a92gh	465	10	PCR	PCR	NNP
cord-009376-a35a92gh	465	11	is	be	VBZ
cord-009376-a35a92gh	465	12	now	now	RB
cord-009376-a35a92gh	465	13	beginning	begin	VBG
cord-009376-a35a92gh	465	14	to	to	TO
cord-009376-a35a92gh	465	15	revolutionise	revolutionise	VB
cord-009376-a35a92gh	465	16	the	the	DT
cord-009376-a35a92gh	465	17	production	production	NN
cord-009376-a35a92gh	465	18	and	and	CC
cord-009376-a35a92gh	465	19	batch	batch	NN
cord-009376-a35a92gh	465	20	release	release	NN
cord-009376-a35a92gh	465	21	of	of	IN
cord-009376-a35a92gh	465	22	products	product	NNS
cord-009376-a35a92gh	465	23	.	.	.
cord-009376-a35a92gh	466	1	The	the	DT
cord-009376-a35a92gh	466	2	high	high	JJ
cord-009376-a35a92gh	466	3	versatility	versatility	NN
cord-009376-a35a92gh	466	4	of	of	IN
cord-009376-a35a92gh	466	5	Q	Q	NNP
cord-009376-a35a92gh	466	6	-	-	HYPH
cord-009376-a35a92gh	466	7	PCR	PCR	NNP
cord-009376-a35a92gh	466	8	assays	assay	NNS
cord-009376-a35a92gh	466	9	coupled	couple	VBN
cord-009376-a35a92gh	466	10	with	with	IN
cord-009376-a35a92gh	466	11	the	the	DT
cord-009376-a35a92gh	466	12	high	high	JJ
cord-009376-a35a92gh	466	13	reproducibility	reproducibility	NN
cord-009376-a35a92gh	466	14	and	and	CC
cord-009376-a35a92gh	466	15	fast	fast	JJ
cord-009376-a35a92gh	466	16	throughput	throughput	NN
cord-009376-a35a92gh	466	17	ability	ability	NN
cord-009376-a35a92gh	466	18	will	will	MD
cord-009376-a35a92gh	466	19	no	no	RB
cord-009376-a35a92gh	466	20	doubt	doubt	RB
cord-009376-a35a92gh	466	21	assist	assist	VB
cord-009376-a35a92gh	466	22	in	in	IN
cord-009376-a35a92gh	466	23	decreasing	decrease	VBG
cord-009376-a35a92gh	466	24	the	the	DT
cord-009376-a35a92gh	466	25	time	time	NN
cord-009376-a35a92gh	466	26	for	for	IN
cord-009376-a35a92gh	466	27	the	the	DT
cord-009376-a35a92gh	466	28	development	development	NN
cord-009376-a35a92gh	466	29	,	,	,
cord-009376-a35a92gh	466	30	safety	safety	NN
cord-009376-a35a92gh	466	31	testing	testing	NN
cord-009376-a35a92gh	466	32	and	and	CC
cord-009376-a35a92gh	466	33	final	final	JJ
cord-009376-a35a92gh	466	34	marketing	marketing	NN
cord-009376-a35a92gh	466	35	of	of	IN
cord-009376-a35a92gh	466	36	novel	novel	JJ
cord-009376-a35a92gh	466	37	biotechnology	biotechnology	NN
cord-009376-a35a92gh	466	38	products	product	NNS
cord-009376-a35a92gh	466	39	.	.	.
cord-009376-a35a92gh	467	1	that	that	IN
cord-009376-a35a92gh	467	2	they	-PRON-	PRP
cord-009376-a35a92gh	467	3	have	have	VBP
cord-009376-a35a92gh	467	4	carried	carry	VBN
cord-009376-a35a92gh	467	5	out	out	RP
cord-009376-a35a92gh	467	6	for	for	IN
cord-009376-a35a92gh	467	7	the	the	DT
cord-009376-a35a92gh	467	8	development	development	NN
cord-009376-a35a92gh	467	9	program	program	NN
cord-009376-a35a92gh	467	10	,	,	,
cord-009376-a35a92gh	467	11	and	and	CC
cord-009376-a35a92gh	467	12	for	for	IN
cord-009376-a35a92gh	467	13	significantly	significantly	RB
cord-009376-a35a92gh	467	14	contributing	contribute	VBG
cord-009376-a35a92gh	467	15	towards	towards	IN
cord-009376-a35a92gh	467	16	solving	solve	VBG
cord-009376-a35a92gh	467	17	the	the	DT
cord-009376-a35a92gh	467	18	problems	problem	NNS
cord-009376-a35a92gh	467	19	encountered	encounter	VBN
cord-009376-a35a92gh	467	20	.	.	.
cord-009376-a35a92gh	468	1	A	a	DT
cord-009376-a35a92gh	468	2	quantitative	quantitative	NN
cord-009376-a35a92gh	468	3	,	,	,
cord-009376-a35a92gh	468	4	internally	internally	RB
cord-009376-a35a92gh	468	5	controlled	control	VBN
cord-009376-a35a92gh	468	6	real	real	JJ
cord-009376-a35a92gh	468	7	-	-	HYPH
cord-009376-a35a92gh	468	8	time	time	NN
cord-009376-a35a92gh	468	9	PCR	PCR	NNP
cord-009376-a35a92gh	468	10	assay	assay	NN
cord-009376-a35a92gh	468	11	for	for	IN
cord-009376-a35a92gh	468	12	the	the	DT
cord-009376-a35a92gh	468	13	detection	detection	NN
cord-009376-a35a92gh	468	14	of	of	IN
cord-009376-a35a92gh	468	15	parvovirus	parvovirus	NN
cord-009376-a35a92gh	468	16	B19	B19	NNP
cord-009376-a35a92gh	468	17	DNA	DNA	NNP
cord-009376-a35a92gh	468	18	Porcine	porcine	JJ
cord-009376-a35a92gh	468	19	circoviruses	circoviruse	NNS
cord-009376-a35a92gh	468	20	:	:	:
cord-009376-a35a92gh	468	21	a	a	DT
cord-009376-a35a92gh	468	22	review	review	NN
cord-009376-a35a92gh	468	23	One	one	CD
cord-009376-a35a92gh	468	24	step	step	NN
cord-009376-a35a92gh	468	25	fluorescent	fluorescent	NN
cord-009376-a35a92gh	468	26	probe	probe	NN
cord-009376-a35a92gh	468	27	product	product	NN
cord-009376-a35a92gh	468	28	enhanced	enhance	VBN
cord-009376-a35a92gh	468	29	reverse	reverse	JJ
cord-009376-a35a92gh	468	30	transcriptase	transcriptase	NN
cord-009376-a35a92gh	468	31	assay	assay	NN
cord-009376-a35a92gh	468	32	Deletion	deletion	NN
cord-009376-a35a92gh	468	33	mutants	mutant	NNS
cord-009376-a35a92gh	468	34	of	of	IN
cord-009376-a35a92gh	468	35	polyoma	polyoma	NN
cord-009376-a35a92gh	468	36	virus	virus	NN
cord-009376-a35a92gh	468	37	defining	define	VBG
cord-009376-a35a92gh	468	38	a	a	DT
cord-009376-a35a92gh	468	39	nonessential	nonessential	JJ
cord-009376-a35a92gh	468	40	region	region	NN
cord-009376-a35a92gh	468	41	between	between	IN
cord-009376-a35a92gh	468	42	the	the	DT
cord-009376-a35a92gh	468	43	origin	origin	NN
cord-009376-a35a92gh	468	44	of	of	IN
cord-009376-a35a92gh	468	45	replication	replication	NN
cord-009376-a35a92gh	468	46	and	and	CC
cord-009376-a35a92gh	468	47	the	the	DT
cord-009376-a35a92gh	468	48	initiation	initiation	NN
cord-009376-a35a92gh	468	49	codon	codon	NN
cord-009376-a35a92gh	468	50	for	for	IN
cord-009376-a35a92gh	468	51	early	early	JJ
cord-009376-a35a92gh	468	52	proteins	protein	NNS
cord-009376-a35a92gh	469	1	Rapid	rapid	JJ
cord-009376-a35a92gh	469	2	quantification	quantification	NN
cord-009376-a35a92gh	469	3	and	and	CC
cord-009376-a35a92gh	469	4	differentiation	differentiation	NN
cord-009376-a35a92gh	469	5	of	of	IN
cord-009376-a35a92gh	469	6	human	human	JJ
cord-009376-a35a92gh	469	7	polyomavirus	polyomavirus	JJ
cord-009376-a35a92gh	469	8	DNA	DNA	NNP
cord-009376-a35a92gh	469	9	in	in	IN
cord-009376-a35a92gh	469	10	undiluted	undiluted	JJ
cord-009376-a35a92gh	469	11	urine	urine	NN
cord-009376-a35a92gh	469	12	from	from	IN
cord-009376-a35a92gh	469	13	patients	patient	NNS
cord-009376-a35a92gh	469	14	after	after	IN
cord-009376-a35a92gh	469	15	bone	bone	NN
cord-009376-a35a92gh	469	16	marrow	marrow	NN
cord-009376-a35a92gh	469	17	transplantation	transplantation	NN
cord-009376-a35a92gh	469	18	Cache	Cache	NNP
cord-009376-a35a92gh	469	19	valley	valley	NN
cord-009376-a35a92gh	469	20	and	and	CC
cord-009376-a35a92gh	469	21	Ž	Ž	NNP
cord-009376-a35a92gh	469	22	.	.	.
cord-009376-a35a92gh	470	1	Potosi	Potosi	NNP
cord-009376-a35a92gh	470	2	viruses	virus	NNS
cord-009376-a35a92gh	470	3	Bunyaviridae	Bunyaviridae	NNP
cord-009376-a35a92gh	470	4	in	in	IN
cord-009376-a35a92gh	470	5	white	white	JJ
cord-009376-a35a92gh	470	6	tailed	tailed	JJ
cord-009376-a35a92gh	470	7	deer	deer	NN
cord-009376-a35a92gh	470	8	Ž	Ž	NNP
cord-009376-a35a92gh	470	9	.	.	.
cord-009376-a35a92gh	471	1	Odocoileus¨irginianus	Odocoileus¨irginianus	NNP
cord-009376-a35a92gh	471	2	:	:	:
cord-009376-a35a92gh	472	1	experimental	experimental	JJ
cord-009376-a35a92gh	472	2	infections	infection	NNS
cord-009376-a35a92gh	472	3	and	and	CC
cord-009376-a35a92gh	472	4	antibody	antibody	NN
cord-009376-a35a92gh	472	5	prevalence	prevalence	NN
cord-009376-a35a92gh	472	6	in	in	IN
cord-009376-a35a92gh	472	7	natural	natural	JJ
cord-009376-a35a92gh	472	8	populations	population	NNS
cord-009376-a35a92gh	472	9	Bunyavirus	Bunyavirus	NNP
cord-009376-a35a92gh	472	10	superinfection	superinfection	NN
cord-009376-a35a92gh	472	11	and	and	CC
cord-009376-a35a92gh	472	12	segment	segment	NN
cord-009376-a35a92gh	472	13	reassortment	reassortment	NN
cord-009376-a35a92gh	472	14	in	in	IN
cord-009376-a35a92gh	472	15	transovarially	transovarially	RB
cord-009376-a35a92gh	472	16	infected	infect	VBN
cord-009376-a35a92gh	472	17	mosquitos	mosquitos	NN
cord-009376-a35a92gh	472	18	Use	use	NN
cord-009376-a35a92gh	472	19	of	of	IN
cord-009376-a35a92gh	472	20	quantitative	quantitative	JJ
cord-009376-a35a92gh	472	21	product	product	NN
cord-009376-a35a92gh	472	22	enhanced	enhance	VBN
cord-009376-a35a92gh	472	23	reverse	reverse	JJ
cord-009376-a35a92gh	472	24	transcriptase	transcriptase	NN
cord-009376-a35a92gh	472	25	assay	assay	NN
cord-009376-a35a92gh	472	26	to	to	TO
cord-009376-a35a92gh	472	27	monitor	monitor	VB
cord-009376-a35a92gh	472	28	retrovirus	retrovirus	NN
cord-009376-a35a92gh	472	29	levels	level	NNS
cord-009376-a35a92gh	472	30	in	in	IN
cord-009376-a35a92gh	472	31	mAb	mab	NN
cord-009376-a35a92gh	472	32	cell	cell	NN
cord-009376-a35a92gh	472	33	-	-	HYPH
cord-009376-a35a92gh	472	34	culture	culture	NN
cord-009376-a35a92gh	472	35	and	and	CC
cord-009376-a35a92gh	472	36	downstream	downstream	JJ
cord-009376-a35a92gh	472	37	processing	processing	NN
cord-009376-a35a92gh	472	38	Cache	cache	NN
cord-009376-a35a92gh	472	39	valley	valley	NN
cord-009376-a35a92gh	472	40	virus	virus	NN
cord-009376-a35a92gh	472	41	infection	infection	NN
cord-009376-a35a92gh	472	42	in	in	IN
cord-009376-a35a92gh	473	1	Texas	Texas	NNP
cord-009376-a35a92gh	473	2	sheep	sheep	NN
cord-009376-a35a92gh	473	3	flocks	flock	NNS
cord-009376-a35a92gh	473	4	Analytical	analytical	JJ
cord-009376-a35a92gh	473	5	method	method	NN
cord-009376-a35a92gh	473	6	validation	validation	NN
cord-009376-a35a92gh	473	7	Real	real	JJ
cord-009376-a35a92gh	473	8	time	time	NN
cord-009376-a35a92gh	473	9	quantitative	quantitative	JJ
cord-009376-a35a92gh	473	10	PCR	pcr	NN
cord-009376-a35a92gh	473	11	for	for	IN
cord-009376-a35a92gh	473	12	retrovirus	retrovirus	NN
cord-009376-a35a92gh	473	13	-	-	HYPH
cord-009376-a35a92gh	473	14	like	like	JJ
cord-009376-a35a92gh	473	15	particle	particle	NN
cord-009376-a35a92gh	473	16	quantification	quantification	NN
cord-009376-a35a92gh	473	17	in	in	IN
cord-009376-a35a92gh	473	18	CHO	CHO	NNP
cord-009376-a35a92gh	473	19	cell	cell	NN
cord-009376-a35a92gh	473	20	culture	culture	NN
cord-009376-a35a92gh	474	1	Ovine	ovine	JJ
cord-009376-a35a92gh	474	2	fetal	fetal	JJ
cord-009376-a35a92gh	474	3	malformations	malformation	NNS
cord-009376-a35a92gh	474	4	induced	induce	VBN
cord-009376-a35a92gh	474	5	by	by	IN
cord-009376-a35a92gh	474	6	utero	utero	NN
cord-009376-a35a92gh	474	7	inoculation	inoculation	NN
cord-009376-a35a92gh	474	8	of	of	IN
cord-009376-a35a92gh	474	9	Main	Main	NNP
cord-009376-a35a92gh	474	10	Drain	Drain	NNP
cord-009376-a35a92gh	474	11	,	,	,
cord-009376-a35a92gh	474	12	San	San	NNP
cord-009376-a35a92gh	474	13	Angelo	Angelo	NNP
cord-009376-a35a92gh	474	14	and	and	CC
cord-009376-a35a92gh	474	15	La	La	NNP
cord-009376-a35a92gh	474	16	Crosse	Crosse	NNP
cord-009376-a35a92gh	474	17	viruses	virus	NNS
cord-009376-a35a92gh	475	1	Cationic	cationic	JJ
cord-009376-a35a92gh	475	2	liposome	liposome	NN
cord-009376-a35a92gh	475	3	-	-	HYPH
cord-009376-a35a92gh	475	4	mediated	mediate	VBN
cord-009376-a35a92gh	475	5	gene	gene	NN
cord-009376-a35a92gh	475	6	transfer	transfer	NN
cord-009376-a35a92gh	475	7	Development	Development	NNP
cord-009376-a35a92gh	475	8	of	of	IN
cord-009376-a35a92gh	475	9	a	a	DT
cord-009376-a35a92gh	475	10	fluorogenic	fluorogenic	JJ
cord-009376-a35a92gh	475	11	Ž	ž	NN
cord-009376-a35a92gh	475	12	.	.	.
cord-009376-a35a92gh	476	1	polymerase	polymerase	NNP
cord-009376-a35a92gh	476	2	chain	chain	NN
cord-009376-a35a92gh	476	3	reaction	reaction	NN
cord-009376-a35a92gh	476	4	assay	assay	NN
cord-009376-a35a92gh	476	5	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	476	6	for	for	IN
cord-009376-a35a92gh	476	7	the	the	DT
cord-009376-a35a92gh	476	8	detection	detection	NN
cord-009376-a35a92gh	476	9	and	and	CC
cord-009376-a35a92gh	476	10	quantification	quantification	NN
cord-009376-a35a92gh	476	11	of	of	IN
cord-009376-a35a92gh	476	12	varcella	varcella	NNP
cord-009376-a35a92gh	476	13	zoster	zoster	NNP
cord-009376-a35a92gh	476	14	virus	virus	NN
cord-009376-a35a92gh	476	15	Real	real	JJ
cord-009376-a35a92gh	476	16	time	time	NN
cord-009376-a35a92gh	476	17	quantitative	quantitative	JJ
cord-009376-a35a92gh	476	18	PCR	PCR	NNP
cord-009376-a35a92gh	476	19	Detection	Detection	NNP
cord-009376-a35a92gh	476	20	of	of	IN
cord-009376-a35a92gh	476	21	reverse	reverse	JJ
cord-009376-a35a92gh	476	22	transcriptase	transcriptase	NN
cord-009376-a35a92gh	476	23	by	by	IN
cord-009376-a35a92gh	476	24	a	a	DT
cord-009376-a35a92gh	476	25	highly	highly	RB
cord-009376-a35a92gh	476	26	sensitive	sensitive	JJ
cord-009376-a35a92gh	476	27	assay	assay	NN
cord-009376-a35a92gh	476	28	in	in	IN
cord-009376-a35a92gh	476	29	sera	sera	NNP
cord-009376-a35a92gh	476	30	from	from	IN
cord-009376-a35a92gh	476	31	persons	person	NNS
cord-009376-a35a92gh	476	32	infected	infect	VBN
cord-009376-a35a92gh	476	33	with	with	IN
cord-009376-a35a92gh	476	34	human	human	JJ
cord-009376-a35a92gh	476	35	immunodeficiency	immunodeficiency	NN
cord-009376-a35a92gh	476	36	virus	virus	NN
cord-009376-a35a92gh	476	37	type	type	NN
cord-009376-a35a92gh	476	38	1	1	CD
cord-009376-a35a92gh	476	39	Sensitive	sensitive	JJ
cord-009376-a35a92gh	476	40	and	and	CC
cord-009376-a35a92gh	476	41	robust	robust	JJ
cord-009376-a35a92gh	476	42	one	one	CD
cord-009376-a35a92gh	476	43	-	-	HYPH
cord-009376-a35a92gh	476	44	tube	tube	NN
cord-009376-a35a92gh	476	45	real	real	JJ
cord-009376-a35a92gh	476	46	-	-	HYPH
cord-009376-a35a92gh	476	47	time	time	NN
cord-009376-a35a92gh	476	48	reverse	reverse	JJ
cord-009376-a35a92gh	476	49	transcriptase	transcriptase	NN
cord-009376-a35a92gh	476	50	-	-	HYPH
cord-009376-a35a92gh	476	51	polymerase	polymerase	NN
cord-009376-a35a92gh	476	52	chain	chain	NN
cord-009376-a35a92gh	476	53	reaction	reaction	NN
cord-009376-a35a92gh	476	54	to	to	TO
cord-009376-a35a92gh	476	55	quantify	quantify	VB
cord-009376-a35a92gh	476	56	SIV	SIV	NNP
cord-009376-a35a92gh	476	57	RNA	RNA	NNP
cord-009376-a35a92gh	476	58	load	load	NN
cord-009376-a35a92gh	476	59	:	:	:
cord-009376-a35a92gh	476	60	comparison	comparison	NN
cord-009376-a35a92gh	476	61	of	of	IN
cord-009376-a35a92gh	476	62	one	one	CD
cord-009376-a35a92gh	476	63	-	-	HYPH
cord-009376-a35a92gh	476	64	versus	versus	IN
cord-009376-a35a92gh	476	65	two	two	CD
cord-009376-a35a92gh	476	66	-	-	HYPH
cord-009376-a35a92gh	476	67	enzyme	enzyme	NN
cord-009376-a35a92gh	476	68	systems	system	NNS
cord-009376-a35a92gh	476	69	Validation	validation	NN
cord-009376-a35a92gh	476	70	of	of	IN
cord-009376-a35a92gh	476	71	Analytical	Analytical	NNP
cord-009376-a35a92gh	476	72	Methods	Methods	NNPS
cord-009376-a35a92gh	476	73	.	.	.
cord-009376-a35a92gh	477	1	Definitions	definition	NNS
cord-009376-a35a92gh	477	2	and	and	CC
cord-009376-a35a92gh	477	3	Terminology	terminology	NN
cord-009376-a35a92gh	477	4	.	.	.
cord-009376-a35a92gh	478	1	The	the	DT
cord-009376-a35a92gh	478	2	European	European	NNP
cord-009376-a35a92gh	478	3	Agency	Agency	NNP
cord-009376-a35a92gh	478	4	for	for	IN
cord-009376-a35a92gh	478	5	the	the	DT
cord-009376-a35a92gh	478	6	Evaluation	Evaluation	NNP
cord-009376-a35a92gh	478	7	of	of	IN
cord-009376-a35a92gh	478	8	Medicinal	Medicinal	NNP
cord-009376-a35a92gh	478	9	Products	Products	NNPS
cord-009376-a35a92gh	478	10	Validation	Validation	NNP
cord-009376-a35a92gh	478	11	of	of	IN
cord-009376-a35a92gh	478	12	Analytical	Analytical	NNP
cord-009376-a35a92gh	478	13	Methods	Methods	NNPS
cord-009376-a35a92gh	478	14	.	.	.
cord-009376-a35a92gh	479	1	Methodolgy	Methodolgy	NNP
cord-009376-a35a92gh	479	2	.	.	.
cord-009376-a35a92gh	480	1	The	the	DT
cord-009376-a35a92gh	480	2	European	European	NNP
cord-009376-a35a92gh	480	3	Agency	Agency	NNP
cord-009376-a35a92gh	480	4	for	for	IN
cord-009376-a35a92gh	480	5	the	the	DT
cord-009376-a35a92gh	480	6	Evaluation	Evaluation	NNP
cord-009376-a35a92gh	480	7	of	of	IN
cord-009376-a35a92gh	480	8	Medicinal	Medicinal	NNP
cord-009376-a35a92gh	480	9	Products	Products	NNPS
cord-009376-a35a92gh	480	10	Detection	Detection	NNP
cord-009376-a35a92gh	480	11	and	and	CC
cord-009376-a35a92gh	480	12	quantitation	quantitation	NN
cord-009376-a35a92gh	480	13	of	of	IN
cord-009376-a35a92gh	480	14	human	human	JJ
cord-009376-a35a92gh	480	15	papillomavirus	papillomavirus	NN
cord-009376-a35a92gh	480	16	by	by	IN
cord-009376-a35a92gh	480	17	using	use	VBG
cord-009376-a35a92gh	480	18	the	the	DT
cord-009376-a35a92gh	480	19	fluorescent	fluorescent	NN
cord-009376-a35a92gh	480	20	5Ј	5ј	CD
cord-009376-a35a92gh	480	21	exonuclease	exonuclease	NN
cord-009376-a35a92gh	480	22	assay	assay	NN
cord-009376-a35a92gh	480	23	Development	Development	NNP
cord-009376-a35a92gh	480	24	of	of	IN
cord-009376-a35a92gh	480	25	a	a	DT
cord-009376-a35a92gh	480	26	TT	TT	NNP
cord-009376-a35a92gh	480	27	virus	virus	NN
cord-009376-a35a92gh	480	28	DNA	dna	NN
cord-009376-a35a92gh	480	29	quantification	quantification	NN
cord-009376-a35a92gh	480	30	system	system	NN
cord-009376-a35a92gh	480	31	using	use	VBG
cord-009376-a35a92gh	480	32	realtime	realtime	JJ
cord-009376-a35a92gh	480	33	detection	detection	NN
cord-009376-a35a92gh	480	34	PCR	PCR	NNP
cord-009376-a35a92gh	480	35	Human	Human	NNP
cord-009376-a35a92gh	480	36	herpes	herpe	NNS
cord-009376-a35a92gh	480	37	virus	virus	NN
cord-009376-a35a92gh	480	38	8	8	CD
cord-009376-a35a92gh	480	39	Ž	Ž	NNP
cord-009376-a35a92gh	480	40	HHV-8	HHV-8	NNP
cord-009376-a35a92gh	480	41	in	in	IN
cord-009376-a35a92gh	480	42	Kaposi	Kaposi	NNP
cord-009376-a35a92gh	480	43	's	's	POS
cord-009376-a35a92gh	480	44	sarcoma	sarcoma	NN
cord-009376-a35a92gh	480	45	:	:	:
cord-009376-a35a92gh	480	46	lack	lack	NN
cord-009376-a35a92gh	480	47	of	of	IN
cord-009376-a35a92gh	480	48	association	association	NN
cord-009376-a35a92gh	480	49	with	with	IN
cord-009376-a35a92gh	480	50	bcl-2	bcl-2	NNP
cord-009376-a35a92gh	480	51	and	and	CC
cord-009376-a35a92gh	480	52	p53	p53	NN
cord-009376-a35a92gh	480	53	expression	expression	NN
cord-009376-a35a92gh	480	54	Quantitative	quantitative	JJ
cord-009376-a35a92gh	480	55	analysis	analysis	NN
cord-009376-a35a92gh	480	56	of	of	IN
cord-009376-a35a92gh	480	57	Epstein	Epstein	NNP
cord-009376-a35a92gh	480	58	᎐	᎐	CD
cord-009376-a35a92gh	480	59	Barr	Barr	NNP
cord-009376-a35a92gh	480	60	virus	virus	NN
cord-009376-a35a92gh	480	61	load	load	NN
cord-009376-a35a92gh	480	62	by	by	IN
cord-009376-a35a92gh	480	63	a	a	DT
cord-009376-a35a92gh	480	64	using	use	VBG
cord-009376-a35a92gh	480	65	real	real	JJ
cord-009376-a35a92gh	480	66	time	time	NN
cord-009376-a35a92gh	481	1	PCR	PCR	NNP
cord-009376-a35a92gh	481	2	assay	assay	NN
cord-009376-a35a92gh	481	3	Comparison	comparison	NN
cord-009376-a35a92gh	481	4	of	of	IN
cord-009376-a35a92gh	481	5	six	six	CD
cord-009376-a35a92gh	481	6	nucleic	nucleic	JJ
cord-009376-a35a92gh	481	7	acid	acid	NN
cord-009376-a35a92gh	481	8	extraction	extraction	NN
cord-009376-a35a92gh	481	9	methods	method	NNS
cord-009376-a35a92gh	481	10	for	for	IN
cord-009376-a35a92gh	481	11	the	the	DT
cord-009376-a35a92gh	481	12	detection	detection	NN
cord-009376-a35a92gh	481	13	of	of	IN
cord-009376-a35a92gh	481	14	viral	viral	JJ
cord-009376-a35a92gh	481	15	DNA	DNA	NNP
cord-009376-a35a92gh	481	16	or	or	CC
cord-009376-a35a92gh	481	17	RNA	RNA	NNP
cord-009376-a35a92gh	481	18	sequences	sequence	NNS
cord-009376-a35a92gh	481	19	in	in	IN
cord-009376-a35a92gh	481	20	four	four	CD
cord-009376-a35a92gh	481	21	different	different	JJ
cord-009376-a35a92gh	481	22	non	non	JJ
cord-009376-a35a92gh	481	23	-	-	JJ
cord-009376-a35a92gh	481	24	serum	serum	JJ
cord-009376-a35a92gh	481	25	specimen	specimen	NN
cord-009376-a35a92gh	481	26	types	type	NNS
cord-009376-a35a92gh	481	27	Retroviruses	retrovirus	NNS
cord-009376-a35a92gh	481	28	in	in	IN
cord-009376-a35a92gh	481	29	rodents	rodent	NNS
cord-009376-a35a92gh	482	1	Quantitative	quantitative	JJ
cord-009376-a35a92gh	482	2	competitive	competitive	JJ
cord-009376-a35a92gh	482	3	reverse	reverse	NN
cord-009376-a35a92gh	482	4	transcription	transcription	NN
cord-009376-a35a92gh	482	5	-	-	HYPH
cord-009376-a35a92gh	482	6	PCR	PCR	NNP
cord-009376-a35a92gh	482	7	as	as	IN
cord-009376-a35a92gh	482	8	a	a	DT
cord-009376-a35a92gh	482	9	method	method	NN
cord-009376-a35a92gh	482	10	to	to	TO
cord-009376-a35a92gh	482	11	evaluate	evaluate	VB
cord-009376-a35a92gh	482	12	retrovirus	retrovirus	NN
cord-009376-a35a92gh	482	13	removal	removal	NN
cord-009376-a35a92gh	482	14	during	during	IN
cord-009376-a35a92gh	482	15	chromatography	chromatography	NN
cord-009376-a35a92gh	482	16	procedures	procedure	NNS
cord-009376-a35a92gh	482	17	Real	real	JJ
cord-009376-a35a92gh	482	18	-	-	HYPH
cord-009376-a35a92gh	482	19	time	time	NN
cord-009376-a35a92gh	482	20	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	482	21	PCR	PCR	NNP
cord-009376-a35a92gh	482	22	as	as	IN
cord-009376-a35a92gh	482	23	a	a	DT
cord-009376-a35a92gh	482	24	specific	specific	JJ
cord-009376-a35a92gh	482	25	and	and	CC
cord-009376-a35a92gh	482	26	more	more	RBR
cord-009376-a35a92gh	482	27	sensitive	sensitive	JJ
cord-009376-a35a92gh	482	28	(	(	-LRB-
cord-009376-a35a92gh	482	29	)	)	-RRB-
cord-009376-a35a92gh	482	30	alternative	alternative	NN
cord-009376-a35a92gh	482	31	to	to	IN
cord-009376-a35a92gh	482	32	the	the	DT
cord-009376-a35a92gh	482	33	branched	branch	VBN
cord-009376-a35a92gh	482	34	-	-	HYPH
cord-009376-a35a92gh	482	35	chain	chain	NN
cord-009376-a35a92gh	482	36	DNA	dna	NN
cord-009376-a35a92gh	482	37	assay	assay	NN
cord-009376-a35a92gh	482	38	for	for	IN
cord-009376-a35a92gh	482	39	quantitation	quantitation	NN
cord-009376-a35a92gh	482	40	of	of	IN
cord-009376-a35a92gh	482	41	Simian	simian	JJ
cord-009376-a35a92gh	482	42	immunodeficiency	immunodeficiency	NN
cord-009376-a35a92gh	482	43	virus	virus	NN
cord-009376-a35a92gh	482	44	RNA	RNA	NNP
cord-009376-a35a92gh	482	45	A	A	NNP
cord-009376-a35a92gh	482	46	defined	define	VBN
cord-009376-a35a92gh	482	47	approach	approach	NN
cord-009376-a35a92gh	482	48	to	to	IN
cord-009376-a35a92gh	482	49	the	the	DT
cord-009376-a35a92gh	482	50	regulatory	regulatory	JJ
cord-009376-a35a92gh	482	51	assessment	assessment	NN
cord-009376-a35a92gh	482	52	of	of	IN
cord-009376-a35a92gh	482	53	the	the	DT
cord-009376-a35a92gh	482	54	use	use	NN
cord-009376-a35a92gh	482	55	of	of	IN
cord-009376-a35a92gh	482	56	neoplastic	neoplastic	JJ
cord-009376-a35a92gh	482	57	cells	cell	NNS
cord-009376-a35a92gh	482	58	as	as	IN
cord-009376-a35a92gh	482	59	substrates	substrate	NNS
cord-009376-a35a92gh	482	60	for	for	IN
cord-009376-a35a92gh	482	61	viral	viral	JJ
cord-009376-a35a92gh	482	62	vaccine	vaccine	NN
cord-009376-a35a92gh	482	63	manufacture	manufacture	NN
cord-009376-a35a92gh	482	64	.	.	.
cord-009376-a35a92gh	483	1	A	a	DT
cord-009376-a35a92gh	483	2	Draft	Draft	NNP
cord-009376-a35a92gh	483	3	.	.	.
cord-009376-a35a92gh	484	1	For	for	IN
cord-009376-a35a92gh	484	2	the	the	DT
cord-009376-a35a92gh	484	3	cell	cell	NN
cord-009376-a35a92gh	484	4	᎐	᎐	NN
cord-009376-a35a92gh	484	5	substrate	substrate	NN
cord-009376-a35a92gh	484	6	᎐	᎐	CD
cord-009376-a35a92gh	484	7	adventitious	adventitious	JJ
cord-009376-a35a92gh	484	8	agent	agent	NN
cord-009376-a35a92gh	484	9	workingrinterest	workingrinterest	NNP
cord-009376-a35a92gh	484	10	group	group	NNP
cord-009376-a35a92gh	484	11	Palindromic	Palindromic	NNP
cord-009376-a35a92gh	484	12	DNA	DNA	NNP
cord-009376-a35a92gh	484	13	and	and	CC
cord-009376-a35a92gh	484	14	genome	genome	NN
cord-009376-a35a92gh	484	15	stability	stability	NN
cord-009376-a35a92gh	484	16	.	.	.
cord-009376-a35a92gh	485	1	Further	further	JJ
cord-009376-a35a92gh	485	2	studies	study	NNS
cord-009376-a35a92gh	485	3	Quantitation	Quantitation	NNP
cord-009376-a35a92gh	485	4	of	of	IN
cord-009376-a35a92gh	485	5	DNArRNA	DNArRNA	NNP
cord-009376-a35a92gh	485	6	using	use	VBG
cord-009376-a35a92gh	485	7	real	real	JJ
cord-009376-a35a92gh	485	8	time	time	NN
cord-009376-a35a92gh	485	9	PCR	PCR	NNP
cord-009376-a35a92gh	485	10	detection	detection	NN
cord-009376-a35a92gh	486	1	Inverted	Inverted	NNP
cord-009376-a35a92gh	486	2	Alu	Alu	NNP
cord-009376-a35a92gh	486	3	repeats	repeat	VBZ
cord-009376-a35a92gh	486	4	unstable	unstable	JJ
cord-009376-a35a92gh	486	5	in	in	IN
cord-009376-a35a92gh	486	6	yeast	yeast	NN
cord-009376-a35a92gh	486	7	are	be	VBP
cord-009376-a35a92gh	486	8	excluded	exclude	VBN
cord-009376-a35a92gh	486	9	from	from	IN
cord-009376-a35a92gh	486	10	the	the	DT
cord-009376-a35a92gh	486	11	human	human	JJ
cord-009376-a35a92gh	486	12	genome	genome	NN
cord-009376-a35a92gh	486	13	High	high	JJ
cord-009376-a35a92gh	486	14	throughput	throughput	NN
cord-009376-a35a92gh	486	15	detection	detection	NN
cord-009376-a35a92gh	486	16	of	of	IN
cord-009376-a35a92gh	486	17	retrovirus	retrovirus	NN
cord-009376-a35a92gh	486	18	-	-	HYPH
cord-009376-a35a92gh	486	19	associated	associate	VBN
cord-009376-a35a92gh	486	20	reverse	reverse	NN
cord-009376-a35a92gh	486	21	transcriptase	transcriptase	NN
cord-009376-a35a92gh	486	22	using	use	VBG
cord-009376-a35a92gh	486	23	an	an	DT
cord-009376-a35a92gh	486	24	improved	improve	VBN
cord-009376-a35a92gh	486	25	Fluorescent	Fluorescent	NNP
cord-009376-a35a92gh	486	26	Product	product	NN
cord-009376-a35a92gh	486	27	Enhanced	Enhanced	NNP
cord-009376-a35a92gh	486	28	Reverse	Reverse	NNP
cord-009376-a35a92gh	486	29	Ž	Ž	NNP
cord-009376-a35a92gh	486	30	Transcriptase	Transcriptase	NNP
cord-009376-a35a92gh	486	31	Assay	Assay	NNP
cord-009376-a35a92gh	486	32	F	F	NNP
cord-009376-a35a92gh	486	33	-	-	HYPH
cord-009376-a35a92gh	486	34	PERT	PERT	NNP
cord-009376-a35a92gh	486	35	and	and	CC
cord-009376-a35a92gh	486	36	its	-PRON-	PRP$
cord-009376-a35a92gh	486	37	comparison	comparison	NN
cord-009376-a35a92gh	486	38	to	to	IN
cord-009376-a35a92gh	486	39	conventional	conventional	JJ
cord-009376-a35a92gh	486	40	detection	detection	NN
cord-009376-a35a92gh	486	41	methods	method	NNS
cord-009376-a35a92gh	486	42	Pharmaceutical	pharmaceutical	JJ
cord-009376-a35a92gh	486	43	perspectives	perspective	NNS
cord-009376-a35a92gh	486	44	of	of	IN
cord-009376-a35a92gh	486	45	nonviral	nonviral	JJ
cord-009376-a35a92gh	486	46	gene	gene	NN
cord-009376-a35a92gh	486	47	therapy	therapy	NN
cord-009376-a35a92gh	486	48	Zoonoses	Zoonoses	NNP
cord-009376-a35a92gh	486	49	and	and	CC
cord-009376-a35a92gh	486	50	haemorrhagic	haemorrhagic	JJ
cord-009376-a35a92gh	486	51	fever	fever	NN
cord-009376-a35a92gh	486	52	Elimination	elimination	NN
cord-009376-a35a92gh	486	53	of	of	IN
cord-009376-a35a92gh	486	54	background	background	NN
cord-009376-a35a92gh	486	55	signals	signal	NNS
cord-009376-a35a92gh	486	56	in	in	IN
cord-009376-a35a92gh	486	57	a	a	DT
cord-009376-a35a92gh	486	58	modified	modify	VBN
cord-009376-a35a92gh	486	59	polymerase	polymerase	NN
cord-009376-a35a92gh	486	60	chain	chain	NN
cord-009376-a35a92gh	486	61	reaction	reaction	NN
cord-009376-a35a92gh	486	62	-	-	HYPH
cord-009376-a35a92gh	486	63	based	base	VBN
cord-009376-a35a92gh	486	64	reverse	reverse	JJ
cord-009376-a35a92gh	486	65	transcriptase	transcriptase	NN
cord-009376-a35a92gh	486	66	assay	assay	NN
cord-009376-a35a92gh	486	67	The	the	DT
cord-009376-a35a92gh	486	68	role	role	NN
cord-009376-a35a92gh	486	69	of	of	IN
cord-009376-a35a92gh	486	70	deer	deer	NN
cord-009376-a35a92gh	486	71	as	as	IN
cord-009376-a35a92gh	486	72	a	a	DT
cord-009376-a35a92gh	486	73	possible	possible	JJ
cord-009376-a35a92gh	486	74	reservoir	reservoir	NN
cord-009376-a35a92gh	486	75	host	host	NN
cord-009376-a35a92gh	486	76	of	of	IN
cord-009376-a35a92gh	486	77	Potosi	Potosi	NNP
cord-009376-a35a92gh	486	78	virus	virus	NN
cord-009376-a35a92gh	486	79	recognised	recognise	VBD
cord-009376-a35a92gh	486	80	arbovirus	arbovirus	NN
cord-009376-a35a92gh	486	81	in	in	IN
cord-009376-a35a92gh	486	82	the	the	DT
cord-009376-a35a92gh	486	83	United	United	NNP
cord-009376-a35a92gh	486	84	States	States	NNP
cord-009376-a35a92gh	486	85	Simultaneous	Simultaneous	NNP
cord-009376-a35a92gh	486	86	screening	screening	NN
cord-009376-a35a92gh	486	87	for	for	IN
cord-009376-a35a92gh	486	88	HBV	HBV	NNP
cord-009376-a35a92gh	486	89	DNA	DNA	NNP
cord-009376-a35a92gh	486	90	and	and	CC
cord-009376-a35a92gh	486	91	HCV	HCV	NNP
cord-009376-a35a92gh	486	92	RNA	RNA	NNP
cord-009376-a35a92gh	486	93	genomes	genome	NNS
cord-009376-a35a92gh	486	94	in	in	IN
cord-009376-a35a92gh	486	95	blood	blood	NN
cord-009376-a35a92gh	486	96	donations	donation	NNS
cord-009376-a35a92gh	486	97	using	use	VBG
cord-009376-a35a92gh	486	98	a	a	DT
cord-009376-a35a92gh	486	99	novel	novel	NN
cord-009376-a35a92gh	486	100	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	486	101	PCR	PCR	NNP
cord-009376-a35a92gh	486	102	Rapid	Rapid	NNP
cord-009376-a35a92gh	486	103	reverse	reverse	JJ
cord-009376-a35a92gh	486	104	transcription	transcription	NN
cord-009376-a35a92gh	486	105	-	-	HYPH
cord-009376-a35a92gh	486	106	PCR	pcr	NN
cord-009376-a35a92gh	486	107	detection	detection	NN
cord-009376-a35a92gh	486	108	of	of	IN
cord-009376-a35a92gh	486	109	hepatitis	hepatitis	NN
cord-009376-a35a92gh	486	110	C	C	NNP
cord-009376-a35a92gh	486	111	virus	virus	NN
cord-009376-a35a92gh	486	112	RNA	RNA	NNP
cord-009376-a35a92gh	486	113	in	in	IN
cord-009376-a35a92gh	486	114	serum	serum	NN
cord-009376-a35a92gh	486	115	using	use	VBG
cord-009376-a35a92gh	486	116	TaqMan	TaqMan	NNP
cord-009376-a35a92gh	486	117	fluorogenic	fluorogenic	JJ
cord-009376-a35a92gh	486	118	detection	detection	NN
cord-009376-a35a92gh	486	119	system	system	NN
cord-009376-a35a92gh	486	120	Development	Development	NNP
cord-009376-a35a92gh	486	121	of	of	IN
cord-009376-a35a92gh	486	122	a	a	DT
cord-009376-a35a92gh	486	123	real	real	JJ
cord-009376-a35a92gh	486	124	-	-	HYPH
cord-009376-a35a92gh	486	125	time	time	NN
cord-009376-a35a92gh	486	126	PCR	pcr	NN
cord-009376-a35a92gh	486	127	procedure	procedure	NN
cord-009376-a35a92gh	486	128	including	include	VBG
cord-009376-a35a92gh	486	129	an	an	DT
cord-009376-a35a92gh	486	130	internal	internal	JJ
cord-009376-a35a92gh	486	131	control	control	NN
cord-009376-a35a92gh	486	132	for	for	IN
cord-009376-a35a92gh	486	133	the	the	DT
cord-009376-a35a92gh	486	134	measurement	measurement	NN
cord-009376-a35a92gh	486	135	of	of	IN
cord-009376-a35a92gh	486	136	HCMV	HCMV	NNP
cord-009376-a35a92gh	486	137	viral	viral	JJ
cord-009376-a35a92gh	486	138	load	load	NN
cord-009376-a35a92gh	487	1	Different	different	JJ
cord-009376-a35a92gh	487	2	real	real	JJ
cord-009376-a35a92gh	487	3	-	-	HYPH
cord-009376-a35a92gh	487	4	time	time	NN
cord-009376-a35a92gh	487	5	PCR	pcr	NN
cord-009376-a35a92gh	487	6	formats	format	NNS
cord-009376-a35a92gh	487	7	compared	compare	VBN
cord-009376-a35a92gh	487	8	for	for	IN
cord-009376-a35a92gh	487	9	the	the	DT
cord-009376-a35a92gh	487	10	quantitative	quantitative	JJ
cord-009376-a35a92gh	487	11	detection	detection	NN
cord-009376-a35a92gh	487	12	of	of	IN
cord-009376-a35a92gh	487	13	human	human	JJ
cord-009376-a35a92gh	487	14	cytomegalovirus	cytomegalovirus	NN
cord-009376-a35a92gh	487	15	DNA	DNA	NNP
cord-009376-a35a92gh	487	16	Human	Human	NNP
cord-009376-a35a92gh	487	17	exposure	exposure	NN
cord-009376-a35a92gh	487	18	to	to	IN
cord-009376-a35a92gh	487	19	bovine	bovine	JJ
cord-009376-a35a92gh	487	20	polyomavirus	polyomavirus	NNP
cord-009376-a35a92gh	487	21	:	:	:
cord-009376-a35a92gh	487	22	a	a	DT
cord-009376-a35a92gh	487	23	zoonosis	zoonosis	NN
cord-009376-a35a92gh	487	24	?	?	.
cord-009376-a35a92gh	488	1	Target	target	NN
cord-009376-a35a92gh	488	2	selection	selection	NN
cord-009376-a35a92gh	488	3	and	and	CC
cord-009376-a35a92gh	488	4	optimisation	optimisation	NN
cord-009376-a35a92gh	488	5	of	of	IN
cord-009376-a35a92gh	488	6	amplification	amplification	NN
cord-009376-a35a92gh	488	7	reactions	reaction	NNS
cord-009376-a35a92gh	488	8	Ultra	ultra	JJ
cord-009376-a35a92gh	488	9	-	-	JJ
cord-009376-a35a92gh	488	10	sensitive	sensitive	JJ
cord-009376-a35a92gh	488	11	retro	retro	JJ
cord-009376-a35a92gh	488	12	-	-	HYPH
cord-009376-a35a92gh	488	13	v	v	NN
cord-009376-a35a92gh	488	14	irus	irus	NNP
cord-009376-a35a92gh	488	15	detection	detection	NN
cord-009376-a35a92gh	488	16	by	by	IN
cord-009376-a35a92gh	488	17	a	a	DT
cord-009376-a35a92gh	488	18	reverse	reverse	JJ
cord-009376-a35a92gh	488	19	transcriptase	transcriptase	NN
cord-009376-a35a92gh	488	20	assay	assay	NN
cord-009376-a35a92gh	488	21	based	base	VBN
cord-009376-a35a92gh	488	22	on	on	IN
cord-009376-a35a92gh	488	23	product	product	NN
cord-009376-a35a92gh	488	24	enhancement	enhancement	NN
cord-009376-a35a92gh	488	25	Critical	critical	JJ
cord-009376-a35a92gh	488	26	factors	factor	NNS
cord-009376-a35a92gh	488	27	for	for	IN
cord-009376-a35a92gh	488	28	successful	successful	JJ
cord-009376-a35a92gh	488	29	PCR	PCR	NNP
cord-009376-a35a92gh	488	30	.	.	.
cord-009376-a35a92gh	489	1	Practical	Practical	NNP
cord-009376-a35a92gh	489	2	Guidelines	Guidelines	NNPS
cord-009376-a35a92gh	489	3	Technical	Technical	NNP
cord-009376-a35a92gh	489	4	Literature	Literature	NNP
cord-009376-a35a92gh	490	1	WHO	who	WP
cord-009376-a35a92gh	490	2	guidelines	guideline	VBZ
cord-009376-a35a92gh	490	3	for	for	IN
cord-009376-a35a92gh	490	4	assuring	assure	VBG
cord-009376-a35a92gh	490	5	the	the	DT
cord-009376-a35a92gh	490	6	quality	quality	NN
cord-009376-a35a92gh	490	7	of	of	IN
cord-009376-a35a92gh	490	8	DNA	dna	NN
cord-009376-a35a92gh	490	9	vaccines	vaccine	NNS
cord-009376-a35a92gh	490	10	Development	Development	NNP
cord-009376-a35a92gh	490	11	of	of	IN
cord-009376-a35a92gh	490	12	a	a	DT
cord-009376-a35a92gh	490	13	high	high	JJ
cord-009376-a35a92gh	490	14	-	-	HYPH
cord-009376-a35a92gh	490	15	throughput	throughput	NN
cord-009376-a35a92gh	490	16	quantitative	quantitative	JJ
cord-009376-a35a92gh	490	17	assay	assay	NN
cord-009376-a35a92gh	490	18	for	for	IN
cord-009376-a35a92gh	490	19	detecting	detect	VBG
cord-009376-a35a92gh	490	20	Herpes	Herpes	NNP
cord-009376-a35a92gh	490	21	simplex	simplex	NN
cord-009376-a35a92gh	490	22	virus	virus	NN
cord-009376-a35a92gh	490	23	DNA	dna	NN
cord-009376-a35a92gh	490	24	in	in	IN
cord-009376-a35a92gh	490	25	clinical	clinical	JJ
cord-009376-a35a92gh	490	26	samples	sample	NNS
cord-009376-a35a92gh	490	27	Application	Application	NNP
cord-009376-a35a92gh	490	28	of	of	IN
cord-009376-a35a92gh	490	29	a	a	DT
cord-009376-a35a92gh	490	30	fluorogenic	fluorogenic	JJ
cord-009376-a35a92gh	490	31	PCR	pcr	NN
cord-009376-a35a92gh	490	32	assay	assay	NN
cord-009376-a35a92gh	490	33	for	for	IN
cord-009376-a35a92gh	490	34	typing	typing	NN
cord-009376-a35a92gh	490	35	and	and	CC
cord-009376-a35a92gh	490	36	subtyping	subtyping	NN
cord-009376-a35a92gh	490	37	of	of	IN
cord-009376-a35a92gh	490	38	influenza	influenza	NN
cord-009376-a35a92gh	490	39	viruses	virus	NNS
cord-009376-a35a92gh	490	40	in	in	IN
cord-009376-a35a92gh	490	41	respiratory	respiratory	JJ
cord-009376-a35a92gh	490	42	samples	sample	NNS
cord-009376-a35a92gh	490	43	Frequent	frequent	JJ
cord-009376-a35a92gh	490	44	detection	detection	NN
cord-009376-a35a92gh	490	45	of	of	IN
cord-009376-a35a92gh	490	46	Bovine	bovine	JJ
cord-009376-a35a92gh	490	47	polyomavirus	polyomavirus	NN
cord-009376-a35a92gh	490	48	in	in	IN
cord-009376-a35a92gh	490	49	commercial	commercial	JJ
cord-009376-a35a92gh	490	50	batches	batch	NNS
cord-009376-a35a92gh	490	51	of	of	IN
cord-009376-a35a92gh	490	52	calf	calf	NN
cord-009376-a35a92gh	490	53	serum	serum	NN
cord-009376-a35a92gh	490	54	by	by	IN
cord-009376-a35a92gh	490	55	using	use	VBG
cord-009376-a35a92gh	490	56	the	the	DT
cord-009376-a35a92gh	490	57	polymerase	polymerase	NN
cord-009376-a35a92gh	490	58	chain	chain	NN
cord-009376-a35a92gh	490	59	reaction	reaction	NN
cord-009376-a35a92gh	490	60	A	a	DT
cord-009376-a35a92gh	490	61	real	real	JJ
cord-009376-a35a92gh	490	62	time	time	NN
cord-009376-a35a92gh	490	63	quantitative	quantitative	JJ
cord-009376-a35a92gh	490	64	PCR	pcr	NN
cord-009376-a35a92gh	490	65	-	-	HYPH
cord-009376-a35a92gh	490	66	based	base	VBN
cord-009376-a35a92gh	490	67	method	method	NN
cord-009376-a35a92gh	490	68	for	for	IN
cord-009376-a35a92gh	490	69	the	the	DT
cord-009376-a35a92gh	490	70	detection	detection	NN
cord-009376-a35a92gh	490	71	and	and	CC
cord-009376-a35a92gh	490	72	quantification	quantification	NN
cord-009376-a35a92gh	490	73	of	of	IN
cord-009376-a35a92gh	490	74	Simian	simian	JJ
cord-009376-a35a92gh	490	75	virus	virus	NN
cord-009376-a35a92gh	490	76	40	40	CD
cord-009376-a35a92gh	490	77	Real	real	JJ
cord-009376-a35a92gh	490	78	time	time	NN
cord-009376-a35a92gh	490	79	quantitative	quantitative	JJ
cord-009376-a35a92gh	490	80	PCR	PCR	NNP
cord-009376-a35a92gh	490	81	as	as	IN
cord-009376-a35a92gh	490	82	a	a	DT
cord-009376-a35a92gh	490	83	method	method	NN
cord-009376-a35a92gh	490	84	to	to	TO
cord-009376-a35a92gh	490	85	evaluate	evaluate	VB
cord-009376-a35a92gh	490	86	Simian	simian	JJ
cord-009376-a35a92gh	490	87	virus	virus	NN
cord-009376-a35a92gh	490	88	40	40	CD
cord-009376-a35a92gh	490	89	removal	removal	NN
cord-009376-a35a92gh	490	90	during	during	IN
cord-009376-a35a92gh	490	91	pharmaceutical	pharmaceutical	JJ
cord-009376-a35a92gh	490	92	protein	protein	NN
cord-009376-a35a92gh	490	93	purification	purification	NN
cord-009376-a35a92gh	490	94	An	an	DT
cord-009376-a35a92gh	490	95	RT	RT	NNP
cord-009376-a35a92gh	490	96	PCR	PCR	NNP
cord-009376-a35a92gh	490	97	assay	assay	NN
cord-009376-a35a92gh	490	98	for	for	IN
cord-009376-a35a92gh	490	99	the	the	DT
cord-009376-a35a92gh	490	100	enzyme	enzyme	NN
cord-009376-a35a92gh	490	101	activity	activity	NN
cord-009376-a35a92gh	490	102	of	of	IN
cord-009376-a35a92gh	490	103	reverse	reverse	JJ
cord-009376-a35a92gh	490	104	transcriptase	transcriptase	NN
cord-009376-a35a92gh	490	105	capable	capable	JJ
cord-009376-a35a92gh	490	106	of	of	IN
cord-009376-a35a92gh	490	107	detecting	detect	VBG
cord-009376-a35a92gh	490	108	single	single	JJ
cord-009376-a35a92gh	490	109	virions	virion	NNS
cord-009376-a35a92gh	491	1	Fast	fast	JJ
cord-009376-a35a92gh	491	2	and	and	CC
cord-009376-a35a92gh	491	3	accurate	accurate	JJ
cord-009376-a35a92gh	491	4	method	method	NN
cord-009376-a35a92gh	491	5	for	for	IN
cord-009376-a35a92gh	491	6	quantitating	quantitate	VBG
cord-009376-a35a92gh	491	7	E.	E.	NNP
cord-009376-a35a92gh	491	8	coli	coli	NNS
cord-009376-a35a92gh	491	9	host	host	NN
cord-009376-a35a92gh	491	10	᎐	᎐	CD
cord-009376-a35a92gh	491	11	cell	cell	NN
cord-009376-a35a92gh	491	12	DNA	dna	NN
cord-009376-a35a92gh	491	13	contamination	contamination	NN
cord-009376-a35a92gh	491	14	in	in	IN
cord-009376-a35a92gh	491	15	plasmid	plasmid	NN
cord-009376-a35a92gh	492	1	DNA	dna	NN
cord-009376-a35a92gh	492	2	preparations	preparation	NNS
cord-009376-a35a92gh	493	1	Plasma	plasma	NN
cord-009376-a35a92gh	493	2	SIV	SIV	NNP
cord-009376-a35a92gh	493	3	RNA	RNA	NNP
cord-009376-a35a92gh	493	4	viral	viral	JJ
cord-009376-a35a92gh	493	5	load	load	NN
cord-009376-a35a92gh	493	6	determination	determination	NN
cord-009376-a35a92gh	493	7	by	by	IN
cord-009376-a35a92gh	493	8	real	real	JJ
cord-009376-a35a92gh	493	9	time	time	NN
cord-009376-a35a92gh	493	10	quantification	quantification	NN
cord-009376-a35a92gh	493	11	of	of	IN
cord-009376-a35a92gh	493	12	product	product	NN
cord-009376-a35a92gh	493	13	generation	generation	NN
cord-009376-a35a92gh	493	14	in	in	IN
cord-009376-a35a92gh	493	15	reverse	reverse	JJ
cord-009376-a35a92gh	493	16	transcriptase	transcriptase	NN
cord-009376-a35a92gh	493	17	polymerase	polymerase	NN
cord-009376-a35a92gh	493	18	chain	chain	NN
cord-009376-a35a92gh	493	19	reaction	reaction	NN
cord-009376-a35a92gh	493	20	Structure	structure	NN
cord-009376-a35a92gh	493	21	and	and	CC
cord-009376-a35a92gh	493	22	distribution	distribution	NN
cord-009376-a35a92gh	493	23	of	of	IN
cord-009376-a35a92gh	493	24	endogenous	endogenous	JJ
cord-009376-a35a92gh	493	25	non	non	JJ
cord-009376-a35a92gh	493	26	-	-	JJ
cord-009376-a35a92gh	493	27	ecotropic	ecotropic	JJ
cord-009376-a35a92gh	493	28	Murine	Murine	NNP
cord-009376-a35a92gh	493	29	leukemia	leukemia	NN
cord-009376-a35a92gh	493	30	virus	virus	NN
cord-009376-a35a92gh	493	31	in	in	IN
cord-009376-a35a92gh	493	32	wild	wild	JJ
cord-009376-a35a92gh	493	33	mice	mouse	NNS
cord-009376-a35a92gh	494	1	Simultaneous	simultaneous	JJ
cord-009376-a35a92gh	494	2	detection	detection	NN
cord-009376-a35a92gh	494	3	of	of	IN
cord-009376-a35a92gh	494	4	influenza	influenza	NN
cord-009376-a35a92gh	494	5	viruses	virus	NNS
cord-009376-a35a92gh	494	6	A	A	NNP
cord-009376-a35a92gh	494	7	and	and	CC
cord-009376-a35a92gh	494	8	B	b	NN
cord-009376-a35a92gh	494	9	using	use	VBG
cord-009376-a35a92gh	494	10	real	real	JJ
cord-009376-a35a92gh	494	11	-	-	HYPH
cord-009376-a35a92gh	494	12	time	time	NN
cord-009376-a35a92gh	494	13	quantitative	quantitative	JJ
cord-009376-a35a92gh	494	14	PCR	PCR	NNP
cord-009376-a35a92gh	494	15	Multiplex	Multiplex	NNP
cord-009376-a35a92gh	494	16	detection	detection	NN
cord-009376-a35a92gh	494	17	of	of	IN
cord-009376-a35a92gh	494	18	four	four	CD
cord-009376-a35a92gh	494	19	pathogenic	pathogenic	JJ
cord-009376-a35a92gh	494	20	retroviruses	retrovirus	NNS
cord-009376-a35a92gh	494	21	using	use	VBG
cord-009376-a35a92gh	494	22	molecular	molecular	JJ
cord-009376-a35a92gh	494	23	beacons	beacon	NNS
cord-009376-a35a92gh	494	24	Correlation	correlation	NN
cord-009376-a35a92gh	494	25	,	,	,
cord-009376-a35a92gh	494	26	regression	regression	NN
cord-009376-a35a92gh	494	27	and	and	CC
cord-009376-a35a92gh	494	28	line	line	NN
cord-009376-a35a92gh	494	29	fitting	fitting	NN
cord-009376-a35a92gh	494	30	through	through	IN
cord-009376-a35a92gh	494	31	graph	graph	NN
cord-009376-a35a92gh	494	32	points	point	NNS
cord-009376-a35a92gh	494	33	:	:	:
cord-009376-a35a92gh	494	34	standard	standard	JJ
cord-009376-a35a92gh	494	35	curves	curve	NNS
cord-009376-a35a92gh	494	36	.	.	.
cord-009376-a35a92gh	495	1	Practical	Practical	NNP
cord-009376-a35a92gh	495	2	Statistics	Statistics	NNPS
cord-009376-a35a92gh	495	3	for	for	IN
cord-009376-a35a92gh	495	4	Experimental	experimental	JJ
cord-009376-a35a92gh	495	5	Biologists	biologist	NNS