id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353389-5dtwje1b	Han, Guojun	Absolute and Relative Quantification of Multiplex DNA Assays Based on an Elemental Labeling Strategy	2013-01-28		.txt	text/plain	3309	174	53	[9] Compared with other methods, it contains two inherent advantages: 1) Owing to the benefits of a large number of elements or isotopes (up to 100) potentially used as elemental tags, as well as excellent mass resolution and multi-element detectors of ICP-MS, high-level multiplexed analysis can be successfully obtained without the limitation of spectral overlap; [10] 2) ICP-MS has allowed isotope ratio measurement with good accuracy and precision, thus in combination with isotope dilution analysis (IDA), absolute-quantitative measurement can be carried out as the complementary use of molecular mass spectrometry. As shown in Scheme 1 a, the procedure of labeling sequence-specific oligonucleotides with elemental tags involves two steps: 1) oligonucleotides, 3' end-functionalized with thiol ( À SH) groups, were specifically derivatized with malemide groups of 1,4,7,10-tetraazacyclododecane-1,4,7tris-aceticacid-10-maleimidoethylacetamide (MMA-DOTA), a compound commonly employed for chemical labeling of proteins or peptides; [17] 2) rare-earth elements (REEs) were chelated with high kinetic and thermodynamic stability (for reaction conditions (pH, mole ratio of MMA-DOTA to DNA, time, and temperature), see the Supporting Information).	./cache/cord-353389-5dtwje1b.txt	./txt/cord-353389-5dtwje1b.txt