id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350004-o151wwcf	An, D. J.	A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs	2008-07-24		.txt	text/plain	3260	169	53	title: A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs PMWS postweaning multisystemic wasting syndrome PDNS porcine dermatitis and nephropathy syndrome PCVAD porcine circovirus associated disease Introduction PCV1 is widespread in the swine population of many countries based on serological surveys (Allan and Ellis 2000) , and is a persistent contaminant of the porcine kidney cell line PK-15 (Tischer et al. Detection is based on a colorimetric reaction following the hybridization of amplified viral DNA that contains a biotinylated nucleotide (biotin-16-dUTP) with oligonucleotide probes that are dotted on a nylon membrane (Quere et al. The objective of this study was to develop a DNA miniarray for the simultaneous detection of PCV1 and PCV2 in pigs with non-PMWS, PMWS and/or PDNS. In conclusion, we have developed a DNA miniarray for simultaneous detection of PCVs that provides similar level of sensitivity (100%) and specificity (98.9%) as in situ hybridization.	./cache/cord-350004-o151wwcf.txt	./txt/cord-350004-o151wwcf.txt