id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328899-kog99kk5	Ferrari, Stefano	Barriers to and new approaches for gene therapy and gene delivery in cystic fibrosis	2002-12-05		.txt	text/plain	10491	536	45	Strategies to overcome these barriers will be addressed in this review and include the use of: (i) clinically relevant adjuncts to overcome the extraand intracellular barriers; (ii) less-conventional delivery routes, such as intravenous or in utero administration; (iii) more efficient non-viral vectors and 'stealth' viruses which can be re-administered; and (iv) new approaches to prolong transgene expression by means of alternative promoters or integrating vectors. Interesting-Using an ex vivo sheep trachea model, we demonly, despite non-viral vectors being arguably less strated that cationic lipid-mediated gene transfer, but efficient than viruses in animal models and labora-not adenoviral vectors or recombinant Sendai virus tory studies, clinical trials have shown that this might [22] , was inhibited by normal mucus, with removal Extracellular barriers include the presence of infected mucus and sputum, mucociliary clearance and tight junctions between the cells, which limit the access of viral vectors to receptors localised on the basolateral membrane.	./cache/cord-328899-kog99kk5.txt	./txt/cord-328899-kog99kk5.txt