id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-323691-5s5almd2	Mishin, Vasiliy P	A ‘minimal’ approach in design of flavivirus infectious DNA	2001-12-04		.txt	text/plain	5060	255	49	Abstract The 'infectious DNA' approach, which is based on in vivo transcription of (+)RNA virus genome cDNA cassettes from eukaryotic promoters in transfected cells, became a popular alternative to the classical scheme in the infectious clone methodology. Substantial difficulties, however, were encountered in design of flavivirus 'infectious DNA', requiring either modification of the viral genome cassette (Yamshchikov et al., 2001) to prevent unwanted expression of viral genome segments encoding toxic for Escherichia coli products, or deletion of the structural protein region (Varnavski et al., 2000) . For this reason we sought to investigate if the stability of constructs containing an unmodified virus genome cassette can be improved by preventing its deleterious expression at the transcriptional level, i.e. by minimizing spurious transcription from eukaryotic promoters in E.	./cache/cord-323691-5s5almd2.txt	./txt/cord-323691-5s5almd2.txt