id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321640-kx3t81yh	Patrone, Paul N.	Affine analysis for quantitative PCR measurements	2020-09-20		.txt	text/plain	7625	458	57	Application to reverse-transcriptase qPCR measurements of a SARS-CoV-2 RNA construct points to the usefulness of this approach for improving confidence and reducing limits of detection in diagnostic testing of emerging diseases. Using this, we develop a data analysis approach employing constrained optimization to determine if an amplification curve exhibits characteristics that are representative of a true signal. We illustrate the validity of this approach on experimental data and demonstrate how it can improve interpretation of late-cycle amplification curves corresponding to low initial DNA concentrations [4] . Our data analysis leverages a universal property of qPCR, which states that under very general conditions, all amplification curves are the same up to an affine transformation, i.e. a multiplicative factor and horizontal shift. To demonstrate that optimization of the transformation parameters does not generate false positives, we performed the analysis described above on 17 non-template control (NTC) datasets that were baseline-corrected according to the same procedure used on the amplification curves.	./cache/cord-321640-kx3t81yh.txt	./txt/cord-321640-kx3t81yh.txt