id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-318609-211m5b79	Yang, Chen	Accurate, rapid and low-cost diagnosis of Mycoplasma pneumoniae via fast narrow-thermal-cycling denaturation bubble-mediated strand exchange amplification	2020-10-11		.txt	text/plain	4723	192	41	Furthermore, ASEA exhibited excellent performance in clinical specimen analysis, with sensitivity and specificity of 96.2% and 100%, respectively, compared with the "gold standard" real-time PCR. pneumoniae nucleic acid testing of clinical specimens to verify the accuracy of this novel method by comparison with real-time PCR, the current "gold standard." The objective was to provide a rapid, low-cost and sensitive detection method with conventional instruments and easily available commercial polymerase for early diagnosis of M. The ASEA reaction was performed in 20 μL amplification mixture containing 2 μL of the relevant templates, 3.0 × 10 −6 M primers F and R, 2 μL ISO buffer, 0.5 μL EvaGreen, 0.2 μL Bst 2.0 WarmStart DNA polymerase and 1.6 μL dNTPs. The amplification mixture was subjected to rapid thermal cycles between a first temperature (T 1 ) and a second temperature (T 2 ) using the CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA).	./cache/cord-318609-211m5b79.txt	./txt/cord-318609-211m5b79.txt