id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-318276-so5jooj0	Bertholet, Christine	Vaccinia virus produces late mRNAs by discontinuous synthesis	1987-07-17		.txt	text/plain	6225	329	60	RNA was isolated from noninfected or infected cells and then hybridized to 32P-labeled probes isolated from the 5' end region, upstream of the 11K coding sequences of cDNA clones 3, 8, and 9 (see Figure 2 ). To confirm this, and to identify the regions from which they are transcribed, the cDNA-derived probes used in the previous experiment were hybridized to cloned DNA restriction fragments representing the entire vaccinia virus genome ( Figure 4 ). %P-labeled DNA probes (~3, ~9, p9, pllK) derived from the 5' region of the corresponding cDNA clones or from genomic DNA (see Figure 2 ) were hybridized lo RNA isolated from uninfected (HeLa) or infected (Early, Late) cells, or to tRNA immobilized on a nitrocellulose membrane. Total late RNA from infected cells protected fragments of 127 and 128 nucleotides of the genomic probe, consistent with the previous experiments, which demonstrated that the sequence complementarity between the genomic DNA and 11K mRNA ends just upstream of the 11K ATG codon.	./cache/cord-318276-so5jooj0.txt	./txt/cord-318276-so5jooj0.txt