id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-317021-o30zylrq	Zhai, Yujia	SmartBac, a new baculovirus system for large protein complex production	2019-02-10		.txt	text/plain	7669	428	56	Here we report SmartBac, an easy and versatile system for constructing large-sized transfer plasmids used to generate recombinant baculoviruses that express large multiprotein complexes in insect cells. The second strategy used to express multiprotein complexes is to construct a transfer plasmid carrying multiple GECs. The commercial pFastbac-Dual vector features two promoters for expression of two proteins simultaneously. In Fig. 2a , a vector is designed to express a multiprotein complex composed of eight different subunits (subunits A, B, C, D, E, F, G and H) in insect cells. Obtaining large multiprotein complexes through recombinant expression has always been challenging for researchers who need a sufficient quantity of high-purity sample for structural and biochemical studies. The key to successful protein production using the baculovirus expression system is the construction of the final transfer plasmid containing genes of multiple protein subunits.	./cache/cord-317021-o30zylrq.txt	./txt/cord-317021-o30zylrq.txt