id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316096-3fnwosst	Jin, Huali	Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice	2005-03-25		.txt	text/plain	4521	222	54	After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-γ, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. All DNA vaccine constructs, encoding the E protein, the M glycoprotein, and the N protein of SARS-CoV, were obtained as follows: these genes were amplified from the cDNA by PCR amplifications using each set of specific primers, respectively. In the present study, it was consistent with their work that the N protein construct could induce the highest SARS-specific IgG, T cell proliferation, and in vivo CTL response (lysis rate of 50.6%) compared with M protein gene (lysis rate of 17%) and E protein gene (lysis rate of 5.6%) (Fig. 4) . In summary, the administrations with all three SARS-CoV DNA vaccines in our study were able to induce high levels of the antigen-specific IgG antibody, the T cell proliferation, IFN-c, DTH, and in vivo CTL responses.	./cache/cord-316096-3fnwosst.txt	./txt/cord-316096-3fnwosst.txt