id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307909-7vbxyns0	Ronda, Luca	Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus	2020-09-02		.txt	text/plain	9356	417	43	We coupled the selectivity and affinity of aptamers, short nucleic acids sequences able to recognize specific epitopes on bacterial surface, immobilized at high density on a nanostructured zirconium dioxide surface, with the rational design of specifically interacting fluorescent peptides to assemble an easy-to-use detection device. λ-Cro is a 66 aa protein that plays a pivotal role in the switch from lysogenic to lytic phase in the growth cycle of phage λ and represented a suitable scaffold for several reasons: (i) the availability of threedimensional structures both in the presence and absence of its cognate DNA (PDB ID: 6cro [60] and 5cro [80] , respectively) allows detailed structural evaluations; (ii) the helix-turn-helix motif of the DNA binding domain is relatively small and all the interactions between the nucleobases of the consensus sequence and the peptidic backbone are well characterized; (iii) the minimum functional portion of the consensus sequence is quite short and made by contiguous nucleobases along the two strands of the DNA target; (iv) previous works reported successful examples of Cro reprogramming for binding consensus sequences that differ from the wild type [79] .	./cache/cord-307909-7vbxyns0.txt	./txt/cord-307909-7vbxyns0.txt