id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307768-xx46w6dc	Ding, Yun	From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis	2017-03-10		.txt	text/plain	9490	506	44	title: From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis Recent applications of such technologies to genetic analysis have suggested significant utility in low-cost, efficient and rapid workflows for DNA amplification, rare mutation detection, antibody screening and next-generation sequencing. 2003) , with the relative concentration of each reagent being defined by the Fig. 1 a Physical and chemical variables in droplet-based experiments: (1) Temperature can be controlled over wide ranges, enabling PCR in emulsions; (2) Hydrophobic substrates or ligands can be delivered through the oil phase into aqueous droplets; (3) Watersoluble components can be delivered through nanoscale droplets or swollen micelles, allowing the regulation of biochemical processes; (4) Internal pH can be altered, for example, by the delivery of acetic acid; (5) Photocaged substrates and ligands can be introduced into the droplets during emulsification and photoactivated at later times. Two recent studies describing single-cell RNA sequencing methods using droplet-based microfluidics [termed Drop-seq (Macosko et al.	./cache/cord-307768-xx46w6dc.txt	./txt/cord-307768-xx46w6dc.txt