id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296197-ohfhnpma	Deborggraeve, Stijn	A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis	2008-11-15		.txt	text/plain	4229	207	47	A phase 1 evaluation of the text was conducted in clinical samples from 60 nonendemic and 45 endemic control subjects and from 44 patients with confirmed cutaneous leishmaniasis (CL), 12 with mucocutaneous leishmaniasis (MCL), and 43 with visceral leishmaniasis (VL) from Peru, Kenya, and Sudan. The Leishmania OligoC-TesT showed high specificity and sensitivity in clinical samples and was able to detect the parasite in samples obtained by less invasive means, such as blood, lymph, and lesion scrapings. Amplification of the parasite DNA by the polymerase chain reaction (PCR) has evolved into one of the most specific and sensitive methods for Leishmania detection [9, 10] . When evaluated in 56 clinical samples from patients with confirmed CL or MCL (definition based on microscopy and/or culture) collected in Peru, the Leishmania OligoC-TesT showed an overall diagnostic sensitivity of 92.9%, whereas all 8 dental biopsy specimens from healthy control subjects were negative.	./cache/cord-296197-ohfhnpma.txt	./txt/cord-296197-ohfhnpma.txt