id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292757-d03byeee	Zhou, Xianfeng	Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly	2007-08-07		.txt	text/plain	4707	237	46	Here, a novel multicomponent supramolecular system involving the preparation of mannose-bearing chitosan oligomers microspheres with entrapping complexes of DNA vaccine and polyethylenimine was developed to mimic many of the beneficial properties of the viruses. After delivery by intramuscular immunization in BALB/c mice, the microspheres induced an enhanced serum antibody responses two orders of magnitude greater than naked DNA vaccine. Here we have designed novel mannose-bearing chitosan oligomers (MBCO) microspheres with entrapping polyethylenimine (PEI)/DNA complexes to mimic the beneficial properties of viruses: Hence, the MBCO microspheres mimic the following five desirable characteristics of a virus: (i) DNA condensation; (ii) cell entry-targeting; (iii) endosome escape; (iv) compact viral size (200-300 nm) and (v) efficient decomplexation. These studies have demonstrated that MBCO microspheres were potent delivery systems for DNA vaccines and are capable of inducing the enhanced humoral and cellular responses (about 100-fold) after i.m. immunization with HBsAg plasmid.	./cache/cord-292757-d03byeee.txt	./txt/cord-292757-d03byeee.txt