id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292569-h8fe0zio	Lee, Si-Hyeong	Preferential production of IgM-secreting hybridomas by immunization with DNA vaccines coding for Ebola virus glycoprotein: use of protein boosting for IgG-secreting hybridoma production	2017-07-26		.txt	text/plain	5460	281	58	title: Preferential production of IgM-secreting hybridomas by immunization with DNA vaccines coding for Ebola virus glycoprotein: use of protein boosting for IgG-secreting hybridoma production These data suggest that immunization with GP DNA vaccines leads to preferential production of hybridomas secreting IgM Abs with little antigen specificity, and that boosting with GP can overcome this propensity. Taken together, these data suggest that boosting with GP could be useful for producing hybridomas that secrete antigen-specific IgG Abs. Six-week-old, female BALB/c mice were purchased from Daehan Biolink (Eumseong, Korea). These data suggest that 11 of the hybridoma clones may secrete IgM Abs with high avidity to mulIgG was purified from cell supernatants using the protein G-resin column (for A6-9). This finding, along with our previous findings, suggests that antigen-specific Ab responses, primed by DNA vaccination, may be boosted by protein immunization, leading to preferential production of IgG-secreting hybridomas.	./cache/cord-292569-h8fe0zio.txt	./txt/cord-292569-h8fe0zio.txt