id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282106-7k088cqv	Yang, Zhi-yong	A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice	2004		.txt	text/plain	3739	193	48	Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Viral replication was reduced by more than six orders of magnitude in the lungs of mice vaccinated with these S plasmid DNA expression vectors, and protection was mediated by a humoral but not a T-cell-dependent immune mechanism. Immunization and challenge were performed in mice as described previously 18 , and viral replication (mean log 10 TCID 50 per g tissue with standard error) in the lower (a) and upper (b) respiratory tract after challenge with SARS-CoV was measured for five immunized animals inoculated with SDCD, SDTM or empty plasmid vector control.	./cache/cord-282106-7k088cqv.txt	./txt/cord-282106-7k088cqv.txt