id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281883-l9yshyc7	Alekseeva, Ekaterina	Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen	2009-06-08		.txt	text/plain	7595	390	45	METHODS: Plasmids encoding core with no translation initiation signal (pCMVcore); with Kozak sequence (pCMVcoreKozak); and with HCV IRES (pCMVcoreIRES) were designed and expressed in a variety of eukaryotic cells. Single immunization with plasmids directing low levels of core expression induced similar levels of cytokines, strong T-cell proliferation (pCMVcoreIRES), and antibodies in titer 10(3)(pCMVcore). Boosting with pCMVcoreKozak induced low antibody response, core-specific T-cell proliferation and IFN- secretion that subsided after the 3rd plasmid injection. Boosting with pCMVcoreKozak induced low antibody response, core-specific T-cell proliferation and IFN- secretion that subsided after the 3rd plasmid injection. Significant responses in the form of core-specific IFN- and IL-2 secretion exceeding the background levels in empty-vector-immunized mice were detected five weeks after a single administration of HCV core gene (Fig.5) . Only the heterologous DNA-prime-protein boost regimen induced a significant core-specific antibody production and potent T-cell response of mainly Th1-profile. Enhancement of cellular and humoral immune responses to hepatitis C virus core protein using DNA-based vaccines augmented with cytokine-expressing plasmids	./cache/cord-281883-l9yshyc7.txt	./txt/cord-281883-l9yshyc7.txt