id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279267-iyobsuvz	Hacker, David L.	Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells	2013-11-30		.txt	text/plain	7064	364	49	Abstract Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery. Currently, the two major approaches to rapid protein production are non-viral transient gene expression (TGE) 1 using mammalian cells [7] [8] [9] [10] [11] and infection of insect cells with a baculovirus expression vector [12, 13] . These cells were used as the host for the TGE method described here because they are efficiently transfected, grow to a high density in suspension culture, and are widely used in the biopharmaceutical industry to generate stable cell lines for the production of therapeutic proteins [1] .	./cache/cord-279267-iyobsuvz.txt	./txt/cord-279267-iyobsuvz.txt