id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276335-e1xlwcvc	Poh, W.P.	Characterization of cytotoxic T‐lymphocyte epitopes and immune responses to SARS coronavirus spike DNA vaccine expressing the RGD‐integrin‐binding motif	2009-05-27		.txt	text/plain	6046	326	52	Significant cell‐mediated immune responses were characterized by cytotoxic T‐lymphocyte (51)Cr release assay and interferon‐gamma secretion ELISPOT assay against RMA‐S target cells presenting predicted MHC class I H2‐Kb epitopes, including those spanning residues 884–891 and 1116–1123 within the S2 subunit of SARS‐CoV spike protein. The production of antigen-specific antibody induced by the SARS-CoV spike DNA vaccinations was assessed For the MHC-peptide binding assay, the mean fluorescence increase (MFI) was calculated as the ratio of the fluorescence of peptide-loaded RMA-S cells to the fluorescence of unloaded RMA-S cells. Mouse IFN-g ELISPOT for splenocytes of C57BL/6 mice immunized with selected S-His and S-RGD/His DNA vaccines to confirm T-cell epitopes of spike protein. This study demonstrated that prime-boost immunization of mice with SARS-CoV spike DNA vaccine constructs S-His and S-RGD/His induced significant antigen-specific cellular immune responses, IFN-g stimulation, and CTL activation.	./cache/cord-276335-e1xlwcvc.txt	./txt/cord-276335-e1xlwcvc.txt