id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276101-quis0c6e	Hamula, Camille L.A.	Selection and analytical applications of aptamers binding microbial pathogens	2011-09-09		.txt	text/plain	4741	259	51	This article describes recent advances in the development of aptamers targeting specific pathogens (e.g., live bacteria, whole viral particles, and virally-infected mammalian cells). Specific aptamers against pathogens have been used as affinity reagents to develop sandwich assays, to label and to image cells, to bind with cells for flow-cytometry analysis, and to act as probes for development of whole-cell biosensors. Aptamers can be chemically modified and incorporated into a variety of simple assays for pathogen detection, as well as more complex assay formats, including flow cytometry, cell imaging, and aptamerbased biosensors. Aptamers binding to the cell surface can be used to purify and to identify their respective target molecules post-SELEX. The aptamers were shown to bind different cell-surface targets via a competitive flow-cytometry experiment; using five aptamers combined, rather than individually, was superior at detecting bacteria in pyogenic fluid.	./cache/cord-276101-quis0c6e.txt	./txt/cord-276101-quis0c6e.txt