id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274049-3gw65kpu	Zhang, Han	CRISPR Editing in Biological and Biomedical Investigation	2017-05-31		.txt	text/plain	6583	333	40	© 2017 Wiley Periodicals, Inc. nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), have enabled the manipulation of genes by targeting DNA double-stranded breaks (DSBs) via non-homologous end-joining (NHEJ) or homology-directed repair (HDR) pathways [Rudin et al., 1989; Rouet et al., 1994; Choulika et al., 1995; Bibikova et al., 2002; Moscou and Bogdanove, 2009 ]. Overall, these studies empower a broader range of disease modeling applications via CRISPR-Cas9-mediated genome engineering, allowing researchers to uncover fundamental mechanisms in disease initiation, maintenance and procession, and explore the therapeutic potential of the CRISPR-Cas9 system to correct disease-causing mutations. Owing to its ability to completely disrupt target genes and the simplicity of designing potent sgRNAs, the CRISPR-Cas9 system has been extended to large-scale loss-of-function (LOF) genome screens in human cells [Koike-Yusa et al., 2014; Shalem et al., 2014; Wang et al., 2014; Zhou et al., 2014] . Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease	./cache/cord-274049-3gw65kpu.txt	./txt/cord-274049-3gw65kpu.txt