id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273993-rkqijcxn	Menchaca, A.	CRISPR in livestock: From editing to printing	2020-01-29		.txt	text/plain	6877	331	42	When applied in large animals, CRISPR involves timeand cost-consuming projects, and it is mandatory not only to choose the best approach for genome editing, but also for embryo production, zygote microinjection or electroporation, cryopreservation and embryo transfer. In addition, we discuss some CRISPR applications to enhance livestock production in the context of a growing global demand of food, in terms of increasing efficiency, reducing the impact of farming on the environment, enhancing pest control, animal welfare and health. The wide range of CRISPR applications in large animals include improving productive traits, enhancing animal welfare through adaptation and resilience, conferring resistance to infectious and transmissible diseases, generating animal models for biomedical research, and suppressing other species considered as pests for livestock. Genome editing mediated by SCNT is applied by some laboratories in some kind of projects (e.g., multiplex editing), but the high efficiency of CRISPR after direct microinjection into zygotes has allowed an easier approach (sheep: [6, 7, 15, 16] ; goats: [9, 17] ; pigs: [11, 13] ).	./cache/cord-273993-rkqijcxn.txt	./txt/cord-273993-rkqijcxn.txt