id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269839-jxqs51o5	Bitome-Essono, Paul-Yannick	Tracking zoonotic pathogens using blood-sucking flies as 'flying syringes'	2017-03-28		.txt	text/plain	5875	319	57	This study demonstrates that using hematophagous flies as 'flying syringes' constitutes an interesting approach to investigate blood-borne pathogen diversity in wild vertebrates and could be used as an early detection tool of zoonotic pathogens. The omnipresence of hematophagous flies in certain habitats and their opportunistic blood-feeding behaviour (Muturi et al., 2011; Muzari et al., 2010; Späth, 2000) make of them compelling candidates to obtain blood meals from different vertebrate hosts for pathogen detection. In the present study, we investigated the possibility of using hematophagous flies as 'flying syringes' to explore the diversity of extant malaria parasites (Haemosporida) infecting wild vertebrates living in the forests of Gabon (Central Africa). Overall, the blood meal origin was successfully identified in 428 fly samples (35%) using a PCR system amplifying long fragments of Cytb (450 bp) or COI genes (330 bp or 660 bp). In this study, we tested whether hematophagous flies could be used as 'flying syringes' to identify blood-borne pathogens circulating in the wild vertebrate fauna of Gabon.	./cache/cord-269839-jxqs51o5.txt	./txt/cord-269839-jxqs51o5.txt