id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259412-l8uta7du	Mattossovich, Rosanna	O(6)-alkylguanine-DNA Alkyltransferases in Microbes Living on the Edge: From Stability to Applicability	2020-04-20		.txt	text/plain	7475	352	42	The reaction mechanism of AGTs is based on the recognition of the damaged nucleobase on DNA [5] , followed by a one-step SN 2 -like mechanism, in which the alkyl group of the damaged guanine is irreversibly transferred to a cysteine residue in its active site [5] [6] [7] [8] (Figure 1 , blue path). These compounds mimic damaged guanine on DNA and react with the protein by the covalent transfer of the alkyl adduct to the active site cysteine residue, thus irreversibly inactivating the enzyme (Figure 1 , red path). Concerning biotechnology, the use of a modified hMGMT as protein tag opened the possibility to generalise this method-a targeted mutagenesis on a thermostable OGT by following a rational approach led to the characterization of SsOGT-H 5 , applicable to in vitro harsh reaction conditions and to in vivo (hyper)thermophilic model organisms.	./cache/cord-259412-l8uta7du.txt	./txt/cord-259412-l8uta7du.txt