id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258363-gmgbus9i	Kolla, Venkatadri	Synthesis of a bacteriophage MB78 late protein by novel ribosomal frameshifting()	2000-08-22		.txt	text/plain	4578	279	63	In vitro transcription-translation yields a major protein that migrates as 28 kDa, whereas in vivo expression using pET expression vectors yields two equally expressed proteins of molecular sizes 28 and 26 kDa. A putative slippery sequence TTTAAAG and a pseudoknot structure, two essential cis elements required for the classical ribosomal frameshifting, are identified in the reading frame. The chelating agent EDTA is an effective It is now known that two proteins can be expressed inhibitor of its DNA synthesis, whereas EGTA and from a single open reading frame through 'ribosomal orthophenanthroline have practically no effect on the frameshifting'. During the process of 'ribosomal frameshifting', two or more proteins can result, starting from a single initiation codon Abbreviations: bp, base pairs; IPTG, isopropyl b-thiogalactosidase; ( Farabaugh and Vimaladithan, 1998 direction (+1 frame shift) has been described in the k The nucleotide sequence reported in this paper has been deposited yeast retrotransposon TY (Belcourt and Farabaugh, in the EMBL/GenBank database under Accession No. X87092.	./cache/cord-258363-gmgbus9i.txt	./txt/cord-258363-gmgbus9i.txt