id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256201-vjzfzshh	Pereira-Gómez, Marianoel	Effect of mismatch repair on the mutation rate of bacteriophage ϕX174	2015-09-10		.txt	text/plain	6108	284	54	Finally, the mutation rate reduction afforded by GATC sites is fully reverted under stress conditions, which up-regulate repair pathways and expression of error-prone host polymerases such as heat and treatment with the base analog 5-fluorouracil, suggesting that access to repair renders the phage sensitive to stress-induced mutagenesis. Finally, we found that the mutation rate reduction afforded by the twenty GATC motifs was fully reverted at 42 C and in the presence of the base analog 5-fluorouracil (5-FU), two stress factors that promote overexpression of repair-associated error prone polymerases (Layton and Foster 2005; Malkova and Haber 2012) , thus suggesting that addition of GATC motifs renders the phage sensitive to stress-induced mutagenesis. We have shown that introduction of GATC sites in the /X174 genome can reduce the spontaneous mutation rate of the phage by up to fiftyfold, indicating that phage DNA can undergo MMR if the required sequence motifs are present.	./cache/cord-256201-vjzfzshh.txt	./txt/cord-256201-vjzfzshh.txt