id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102866-40s64455	Bhadra, Sanchita	One enzyme reverse transcription qPCR using Taq DNA polymerase	2020-05-30		.txt	text/plain	2863	161	58	To verify this activity and to determine whether Taq polymerasemediated reverse transcription might be leveraged for single enzyme RNA detection, we carried out the CDC-approved SARS-CoV-2-specific N1 TaqMan RT-qPCR assay using only Taq DNA polymerase and its accompanying commercial reaction buffer, ThermoPol, (New England Biolabs) seeded with different copies of N gene armored RNA (Asuragen), a commercial template preparation that is devoid of DNA. Even though the only polymerase present in these reactions was Taq DNA polymerase (NEB), and no dedicated reverse transcriptase was added, amplification curves were generated in response to 3 x 10 5 , 3 x 10 4 , and 3 x 10 3 copies of the SARS-CoV-2 N gene armored RNA templates (Figure 1 ). Similar to our results with armored N gene RNA, the NEB Taq DNA polymerase was able to perform TaqMan RT-qPCR analysis of SARS-CoV-2 viral genomic RNA with all three CDC assays (Figure 2 ).	./cache/cord-102866-40s64455.txt	./txt/cord-102866-40s64455.txt