id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102504-d840uu3e	Hass, Kenneth N.	Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing	2020-03-20		.txt	text/plain	4432	285	57	This miniaturized and fully packed IMPACT chip demonstrates rapid, sensitive, and simple nucleic acid detection and is an ideal candidate for the next generation molecular diagnostic platform for point-of-care (POC) applications, responding to emerging and deadly pathogen outbreaks. On the solid surface, reporter probes do not require a quencher since they are only measured in the liquid phase after degradation, thus no fluorescent signal will be detected without the target DNA present in the assay. Leveraging the high activity of CRISPR-Cas12a enzyme and the ability of micropillars to bind more reporter probes, we successfully detect double-stranded DNA target without background issues. As shown in Fig. 3 , for streptavidin coated surface, the number of DNA immobilized on the surface does not show significant change with an incubation time between 10 to 60 min as the integrated fluorescence intensity of the retrieved DNA ranges between 50,000 to 60,000 counts. The extended surface provided by high-aspect ratio micropillars significantly increases the reporter probe binding capacity.	./cache/cord-102504-d840uu3e.txt	./txt/cord-102504-d840uu3e.txt