id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102370-5uy8dq18	Marano, Jeffrey M.	Rolling Circle Amplification is a high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones	2020-06-23		.txt	text/plain	4116	225	53	The traditional approach to rescuing virus from an infectious cDNA clone requires propagating large amounts of plasmids in bacteria, which can result in unwanted mutations in the viral genome due to bacterial toxicity or recombination and requires specialized equipment and knowledge to propagate the bacteria. We have developed an alternative method of preparing large quantities of DNA that can be directly transfected to recover infectious virus without the need for bacteria by amplifying the infectious cDNA clone plasmid using rolling circle amplification (RCA). Typically, the propagation of infectious cDNA clones before viral rescue requires the generation of high concentration plasmid stocks from bacteria, which is not only cumbersome and time-consuming but also presents an opportunity for the introduction of unwanted mutations during amplification in bacteria. To ensure that the above results were not restricted to a specific RCA kit, Vero cells were transfected in triplicate in two independent replicates with RCA product produced using both the Evomics SuperPhi Kit and the GE GenomiPhi Kit or plasmid DNA (Fig. 4) .	./cache/cord-102370-5uy8dq18.txt	./txt/cord-102370-5uy8dq18.txt