id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-032220-u5oo7mj2	Bao, Mengdi	Magnetic Bead-Quantum Dot (MB-Qdot) Clustered Regularly Interspaced Short Palindromic Repeat Assay for Simple Viral DNA Detection	2020-09-04		.txt	text/plain	4825	300	52	[Image: see text] We have developed a novel detection system that couples clustered regularly interspaced short palindromic repeat-Cas recognition of target sequences, Cas-mediated nucleic acid probe cleavage, and quantum dots as highly sensitive reporter molecules for simple detection of viral nucleic acid targets. 18, 19 However, most of the CRISPR-Cas detection systems utilize reporter probes with organic dyes and quenchers that require external instruments and possess a high fluorescence background, which limits overall sensitivity. 20 In this study, we develop a novel probe system for CRISPR-Cas nucleic acid assays that use quantum dots (Qdots) as a reporter. After magnetic isolation, a high fluorescence intensity (∼14 counts) was detected, indicating that the MB-Qdot conjugate is mainly achieved by DNA complementary hybridization rather than non-specific absorption (control, blue). To avoid bulky and complicated sensing instruments, in this work, we developed a simple visual detection system coupled with quantum dots as an ultra-brightness indicator and CRISPR-Cas12a assay for isothermal viral DNA target sensing.	./cache/cord-032220-u5oo7mj2.txt	./txt/cord-032220-u5oo7mj2.txt