id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-021063-4y8m33ea	Hug, Peter	Chapter 18 The advantages of liposome-based gene therapy: A comparison of viral versus liposome-based gene delivery	2007-09-02		.txt	text/plain	6270	341	51	Potential problems associated with the use of viral vectors include: (i) the possibility of recombination events that could convert a replication-defective vector into an infectious agent, (ii) the possibility that superinfection with another retrovirus may allow unwanted transfer of the introduced gene between individuals, (iii) a 7-13 kb limit on the amount of DNA that can be packaged, (iv) potential problems in targeting the virus to specific cells, and (v) difficulty in maintaining high-level expression of the exogenous gene. While both cationic liposomes and retroviral gene delivery vectors lack the ability to target specific cells, the lack of length constraints makes tissue-specific expression easier to achieve using DNA-lipid complexes as compared to retroviruses. Second, limiting the number of cells that are transfected reduces the amount of DNA, lipid, and other proteins needed to perform the gene therapy. The central problem of any liposome-based gene therapy system is that DNA must be introduced into the cytoplasm of the target cell.	./cache/cord-021063-4y8m33ea.txt	./txt/cord-021063-4y8m33ea.txt