id author title date pages extension mime words sentences flesch summary cache txt cord-016628-ljzsg9up Bajpai, Bhakti High Capacity Vectors 2013-10-22 .txt text/plain 4070 215 52 An ideal cloning vehicle would have the following four properties: • Low-molecular weight • Ability to confer readily selectable phenotypic traits on host cells • Single sites for a large number of restriction endonucleases, preferably in genes with a scorable phenotype • Ability to replicate within the host cell, so that numerous copies of the recombinant DNA molecule can be produced and passed to daughter cells. The examples of naturally occurring or artificially constructed vectors include vectors based on Escherichia coli plasmids, bacteriophages (e.g., k, M13, P1), viruses (e.g., animal viruses-retrovirus, adenovirus, adeno-associated virus, Herpes Simplex virus, Vaccinia virus, etc.; insect viruses-baculo virus; plant viruses-cauliflower mosaic virus, potato virus X, Gemini virus, etc.), Agrobacterium tumefaciens based vectors, chimeric plasmids (e.g., cosmid, phagemid, phasmid, and fosmid), artificial chromosomes [e.g., YAC, BAC, PAC, MAC and HAC], and non-E. ./cache/cord-016628-ljzsg9up.txt ./txt/cord-016628-ljzsg9up.txt