id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-013290-j3assowx	Guibinga, Ghiabe H.	Protection against Borreliella burgdorferi infection mediated by a synthetically engineered DNA vaccine	2020-08-12		.txt	text/plain	5081	281	50	We describe the development of a novel synthetically engineered DNA vaccine, pLD1 targeting the outer-surface protein A (OspA) of Borreliella burgdorferi. Immunization of C3 H/HeN mice with pLD1 elicits robust humoral and cellular immune responses that confer complete protection against a live Borreliella burgdorferi bacterial challenge. The data show that pDL1 DNA vaccine elicits robust humoral and cellular immune responses in mice against the OspA antigen that confers protection against Borreliella burgdorferi spirochete tissue colonization following a live bacterial challenge. We show that a synthetically engineered vaccine pLD1 elicits robust and durable anti-OspA IgG levels, and confers protection against Borreliella burgdorferi infection in a C3 H/HeN mouse needle challenge model. These data indicate the immune response elicited by pLD1 can generate antibodies which can bind to the same OspA epitopes as mAbs which have been shown to prevent transmission of Lyme disease spirochetes.	./cache/cord-013290-j3assowx.txt	./txt/cord-013290-j3assowx.txt