id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007757-4mri8kyq	van de Sluis, Bart	Transgene Design	2010-10-04		.txt	text/plain	3791	205	43	Size constraints, inherent to particular cloning systems, may limit the use of native regulatory elements: if a transgene becomes too large for regular plasmid or cosmid-based vectors, or when genetic complementation is desired (e.g., with DNA fragments spanning large genomic deletions), one can switch to systems that allow cloning of very large DNA segments (see Subheading 1.3; Chapter 9). In addition, some endogenous introns appear to harbor regulatory elements with structural and functional similarities to enhancers, Locus control regions (LCRs), or Matrix attachment regions (MARs) (see Subheading 2.2) which direct transgene expression in a position-independent or cell type-specific fashion (9-17). The experimenter has a certain degree of freedom to tailor transgene design to specific requirements ((3); see also The choice of regulatory elements that drive transgene expression is broad (Fig. 2) , and is primarily determined by the aim of the model.	./cache/cord-007757-4mri8kyq.txt	./txt/cord-007757-4mri8kyq.txt