id author title date pages extension mime words sentences flesch summary cache txt cord-004561-cer5ifac Astua-Monge, G. Evidence for a prokaryotic insertion-sequence contamination in eukaryotic sequences registered in different databases 2002 .txt text/plain 3807 200 52 An insertion-sequence of prokaryotic origin was detected in a genomic clone obtained from a Phaseolus vulgaris bacterial artificial chromosome (BAC) library. Southern hybridization of EcoRI-digested BnBAC25 and BnBAC55 clones vulgaris BAC clones resulting from the insertion of the prokaryotic transposable element IS10R. C A southern-blot of several BAC clones showing that only the mutant BnBAC55::IS10R has a 4.1-kb fragment that hybridizes to a probe derived from the IS10R sequence. The presence of the 2.3-kb EcoRV fragment in all clones after prolonged subculturing suggested that IS10R Fig. 2 Sample BAC filter-hybridization results with the IS10R sequence as a probe. BAC DNA prepared from the intensely hybridizing clones possesses a common restriction fragment that contains part of the IS10R sequence database sequence entries from diverse eukaryotic sources that include humans, Arabidopsis, yeast and Drosophila, among others. ./cache/cord-004561-cer5ifac.txt ./txt/cord-004561-cer5ifac.txt