id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003674-3ajyr5e4	NAGAO, Konomu	Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection	2019-03-27		.txt	text/plain	2565	130	53	title: Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection A fluorescent loop-mediated isothermal amplification (fLAMP) assay targeting BLV env sequences was developed and used to evaluate 100 bovine blood samples. Fluorescent (fLAMP) reagents are commercially available, and this real-time amplification approach allows extremely rapid and accurate diagnosis through the use of an improved chain replacement enzyme and annealing analysis compared with tLAMP [4, 6, 17] . Here we used 100 bovine blood samples obtained from farms in the Kagoshima, Miyazaki and Oita prefectures in Japan to develop an fLAMP assay that we compared with a published real-time PCR assay. We used 100 bovine clinical blood samples, comprising 80 ELISA-positive and 20 ELISA-negative samples, to evaluate the performance of the BLV specific fLAMP assay. Development of loop-mediated isothermal amplification method for diagnosis of bovine leukemia virus infection	./cache/cord-003674-3ajyr5e4.txt	./txt/cord-003674-3ajyr5e4.txt