id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349066-546ozkly	Walker, D.H.	Principles of Diagnosis of Infectious Diseases	2014-08-21		.txt	text/plain	2943	137	34	The methods of detection include cultivation of bacteria and fungi on growth medium, isolation of viruses in cell culture, and identification of the agent biochemically, antigenically, or genetically. Visualization of an agent in infected tissue can provide a diagnosis based on specific morphological characteristics or identify the category of organism, for example, gram-positive or gram-negative bacterium or virus (e.g., eosinophilic cytoplasmic inclusion bodies in neurons in rabies virus infection). Specific diagnoses require isolation of the agent in culture, microscopic visualization of the pathogen in tissue lesions, and/or detection of a specific host immune response to the organism. Identification of fungi has been accelerated greatly in microbiology laboratories by performing either hybridization tests or polymerase chain reaction (PCR) on media growing a fungus that is not identifiable by conventional morphological techniques such as blood culture bottles that contain yeast growth.	./cache/cord-349066-546ozkly.txt	./txt/cord-349066-546ozkly.txt