id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331584-z43ifmr3	Mahy, B.W.J.	Emerging and Reemerging Virus Diseases of Vertebrates	2008-07-30		.txt	text/plain	3984	160	49	The threat of a new pandemic of influenza virus in the human population stresses the need for development of better methods for detection, surveillance, and control of emerging virus diseases. Although it is still important to isolate viruses in cell culture for their complete characterization, it is now possible directly to detect viruses in diseased tissues by PCR, then, by sequencing the amplicon, to determine whether a new virus has emerged to cause the disease. For example, when hantavirus pulmonary syndrome, caused by a bunyavirus of rodents, Sin Nombre virus, was initially detected in 1993 in the Four Corners region of Western USA, it was found that rodents inside a house where people had been infected carried a virus identical in sequence to virus isolated from human cases. Then, in 1993, a new hantavirus emerged in the Four Corners region of Southwestern USA as the cause of a severe acute respiratory disease syndrome, with a fatality rate close to 40%, and named Sin Nombre virus.	./cache/cord-331584-z43ifmr3.txt	./txt/cord-331584-z43ifmr3.txt