id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293421-0ksn0fc7	Rodriguez, J. M.	Detection of animal pathogens by using the polymerasechain reaction (PCR)	1997-05-31		.txt	text/plain	9106	559	49	Summary The polymerase chain reaction (PCR) is a nucleic acid-based technique that enables the rapid and sensitive detection of specific micro-organisms. Althougla PCR has some shortcomings, such as the problems caused by contaminants and inhibitors or the lack of suitable sequences for designing specific primers, the outstanding research effort focused on tiffs technique, together with the remarkable development of molecular biology have minimized the deficiencies and allowed its increased general use as a diagnostic tool. Sensitive studies using reference strains of BVDV fi-om persistently infected carriers have shown that reverse transo-iption (RT)-PCR has greater sensitivity than other tests, including enzyme-linked immunosorbent assay (ELISA) (Horner el aL, 1995) ; unfortunately, cost currently makes this technique unsuitahle for large-scale testing but it should be valuahle as a coniirmatm T test in cases where ELISA resuhs are in the 'suspicious range' or where the viral titre is low, such as in batches of foetal bovine serum. Comparison of polymerase chain reaction and virus isolation for detection of epizootic hemorrhagic disease in clinical samples from naturally infected deer	./cache/cord-293421-0ksn0fc7.txt	./txt/cord-293421-0ksn0fc7.txt