id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279694-25rblhwb	Mahy, B.W.J	Emerging and Reemerging Virus Diseases of Vertebrates	2014-11-28		.txt	text/plain	4322	177	50	Although it is still important to isolate viruses in cell culture for their complete characterization, it is now possible directly to detect viruses in diseased tissues by PCR, then, by sequencing the amplicon, to determine whether a new virus has emerged to cause the disease. For example, when hantavirus pulmonary syndrome, caused by a bunyavirus of rodents, Sin Nombre virus, was initially detected in 1993 in the Four Corners region of Western USA, it was found that rodents inside a house where people had been infected carried a virus identical in sequence to virus isolated from human cases. Then, in 1993, a new hantavirus emerged in the Four Corners region of Southwestern USA as the cause of a severe acute respiratory disease syndrome, with a fatality rate close to 40%, and named Sin Nombre virus. It will be important in the future to detect new viruses before they can emerge to cause disease in the population.	./cache/cord-279694-25rblhwb.txt	./txt/cord-279694-25rblhwb.txt