cord-002085-e7xwb03g	2016	We constructed a DENV database containing the serotype, genotype, year and country/region of collection by collecting all publically available DENV sequence information from the National Center for Biotechnology Information (NCBI) and assigning genotype information. DGV also assigns the serotype and genotype to a user-specified sequence by performing a homology search against the curated DENV database, and shows its homologous sequences with the geographical position and year of collection. The second database is the Dengue virus genotyping database 2 (Yamashita et al., 2013) , which provides a summary table containing the DENV serotype/genotype, year and country of collection and accession number. The Dengue Virus Resource 3 facilitates the retrieval of DENV sequences deposited in GenBank according to serotype, disease symptom, host, region/country, genome region, and collection and/or release data (Resch et al., 2009) . DGV provides a search engine for the assignment of the DENV serotype, genotype, and origin country according to the most homologous sequence on the basis of a blastn search against the DENV database.
cord-002179-v8lpw4r7	2016	title: Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements Previously, we have described a high-throughput mass spectrometry method termed TUX-MS (thiouracil cross-linking mass spectrometry) to identify host factors that interact with viral RNA during a live infection in cell culture [15] . Development of a quantitative thiouridine cross-linking mass spectrometry (qTUX-MS) method for identification of proteins associated with the DENV RNA TUX-MS can be used to identify host factors by incorporating 4-thiouridine (4sU), a zero-distance cross-linker, into the viral RNA (vRNA) to enable cross-linking of proteins bound to vRNA during a live infection in cell culture [15] . Since some of hnRNPs are known to modulate cellular gene expression in response to dengue infection [60, 61] , we can not rule out that some of the observed effects on virus titers derive from their roles in regulating host mRNAs. Among the numerous qTUX-MS identified factors of interest, our study is the first to demonstrate the involvement of HMCES (or C3orf37) in viral infection.
cord-002413-795wuqz5	2017	title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 IRAV is an RNA binding protein and localizes to cytoplasmic processing bodies (P bodies) in uninfected cells, where it interacts with the MOV10 RISC complex RNA helicase, suggesting a role for IRAV in the processing of viral RNA. FLJ11286, which we refer to here as IRAV (interferon-regulated antiviral gene) (also annotated as C19orf66, UPF0515, or RyDEN), encodes a protein 291 amino acids (aa) in length with a calculated molecular mass of 33.1 kDa. Analysis of published microarray data suggests that IRAV (FLJ11286) is upregulated in response to type I and type II IFNs (6, 20, (24) (25) (26) . IRAV also associates with the host RNA binding proteins UPF1 and HuR (ELAV1) and interacts with MOV10 (a RISC complex RNA helicase), suggesting a role for IRAV in processing or stability of RNA.
cord-002426-5e1xn7kj	2017	MATERIALS AND METHODS: We carried out a cohort-nested, case-control study with 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with the goal of describing human mobility patterns of recently Dengue virus-infected subjects, and comparing them with those of non-infected subjects living in an urban endemic locality. CONCLUSIONS: Results of this study show that human mobility in a small urban setting exceeded that considered by local health authority''s administrative limits, and was different between recently infected and non-infected subjects living in the same household. These observations provide important insights about the role that human mobility may have in Dengue virus transmission and persistence across endemic geographic areas that need to be taken into account when planning preventive and control measures. Sample: 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with age older than 12, and residents in Axochiapan, Morelos State, México, were selected from the cohort "Peridomestic infection as determinant for Dengue virus transmission" [13] .
cord-002581-r7mskri0	2017	
cord-011012-5mev3otu	2020	
cord-011026-iapgkz0p	2019	title: Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus Smp76 antiviral activity was evaluated using a cell culture technique utilizing Huh7it-1, Vero/SLAM, HCV (JFH1, genotype 2a) and DENV (Trinidad 1751, type 2). For dengue virus infectivity, serially diluted venom fractions and Smp76 were mixed with fixed amount of DENV and incubated for 2 h at 37 °C. The above results suggest that the Smp76 directly affects HCV particles and/or host cells in the culture medium to inhibit the viral infection and does not have an antiviral effect in the cells. To determine whether the antiviral activity of Smp76 peptide (previously described) was specific to HCV and DENV, a Schematic of infection assay. The exact mechanism by which Smp76 exerts its antiviral activity against HCV and DENV to inhibit infecting their target cells need further studies.
cord-011390-98xyie7s	2020	An outbreak of dengue-like illness (DLI) that occurred in April 2016 prompted this study, which aimed to determine the population''s immunity status and identify the arboviruses circulating in the country. The study objectives were to estimate the seroprevalence of arboviruses in the urban population of Solomon Islands while using qPCR to determine the circulating viruses from the clinical samples; furthermore, to characterize and compare the dengue virus isolated from our study to previous published isolates from the region using the phylogenetic tree analysis. The high positivity rate for anti-DENV IgG antibodies (77.9%) and the high DENV positivity rate detected in the qPCR analysis of the clinical samples (76.3%) were indicative of a high number of secondary infections in the 2016 outbreak. The phylogenetic tree analysis and the serotypespecific qPCR results indicated that there was a single DENV-3 serotype in circulation from 2012 to 2016 in Solomon Islands.
cord-011968-abd891ej	2020	Macrophage migration inhibitory factor (MIF) is a pleiotropic proinflammatory cytokine that mediates diverse immune responses, and the serum level of MIF positively correlates with disease severity in patients with dengue. In another study, it was demonstrated that live DENV2-induced endoplasmic reticulum (ER) stress is required for autophagy activation, viral replication and pathogenesis in HuH-7 and A549 cells [57] . In our study, since UV-inactivated viral particles could not induce MIF secretion or expression, it is possible that DENV infection triggered RNA sensing or pattern recognition receptor (PRR) activation, which was followed by the release of preformed MIF from the cytosol through the vesicle trafficking secretory pathway. Macrophage migration inhibitory factor induced by dengue virus infection increases vascular permeability Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy Dengue virus nonstructural protein 1 induces vascular leakage through macrophage migration inhibitory factor and autophagy
cord-025181-eg108wcd	2020	
cord-033331-giku34r9	2020	
cord-253480-qchrw337	2016	Here, we found that the production of infectious DENV particles was significantly decreased by CBX treatment in DENV‐permissive cells, while the viral RNA and viral protein synthesis were not affected. Moreover, results from time-of-addition study showed that the inhibitory effect of CBX on DENV was exhibited by targeting the virus itself, not the host cells. In this study, we investigated whether CBX treatment inhibits dengue infection by measuring the production of progeny virions and viral RNA as well as viral protein expression. To determine whether CBX inhibits the production of infectious progeny DENV in other dengue-permissive cells, TCID 50 assay was performed to measure the virus titer in CBX-treated THP-1 and HUVEC cells, both of which are widely used in DENV studies [Halstead, 1988; Wu et al., 2000] . CBX treatment did not inhibit DENV-1 or DENV-2 RNA synthesis and protein expression in one replication cycle, but markedly reduced progeny virus The total RNA was isolated from the infected cells and analyzed by quantitative RT-PCR.
cord-256732-md1u51va	2020	Inflammasome plays a vital function in the host innate immune system by regulating the secretion of proinflammatory cytokines (IL-1β and IL-18) and subsequent induction of "pyroptosis, " a form of programmed cell death, activated by inflammatory caspases (Fink and Cookson, 2006; Lamkanfi and Dixit, 2014) . IL-1β secretion further enhances the production and release of IL-23 and IL-6 and the association of IL-18, IL-1β, and IL-23 stimulate Th17/γδ T cells to generate pro-inflammatory cytokines (GM-CSF, IL-17A, IL-17F, IL-22,) that create the stage for host adaptive immune responses during DENV infection (Wu et al., 2013a) . Another study demonstrated that DENV infects DC and induces NLRP3 inflammasome activation, triggering ROS production in mitochondria that leads to the discharge of mitochondrial DNA (mtDNA) into the cytosol. (D) DENV activates platelets and induces the secretion of EVs (DV-EXOs and DV-MVs), that results in the CLEC5A and TLR2 mediated release of proinflammatory cytokines and NETs formation in neutrophils contributing to vascular leakage during dengue infection.
cord-270495-2u072mtp	2020	When SARS-CoV-2 is negative and clinical indication is present (at least fever and thrombocytopenia), DENV NS1 antigen and/or IgM/IgG antibody testing may be performed. Clinicians from Singapore reported two COVID-19 cases that were misdiagnosed as dengue among patients who presented with clinical manifestations and hematology profiles, suggesting dengue infection and false-positive DENV IgM antibody using a rapid diagnostic test (RDT). COVID-19 cases were defined as inpatients who met the COVID-19 criteria based on a predetermined combination of symptoms, laboratory testing, imaging, and risk exposure at Tangerang District Hospital, Indonesia (see Supplemental Table 1 ), and had a positive nasopharyngeal or oropharyngeal real-time RT-PCR for SARS-CoV-2. None of the 42 subjects was positive for dengue NS1 or showed seroconversion or increasing DENV IgM and IgG index values, suggesting no acute DENV infection among these COVID-19 cases. The third patient did not recall having a fever before acute COVID-19 illness, suggesting asymptomatic or mild dengue, the most common presentation of DENV infection.
cord-272459-w14finxf	2011	Recently, it was reported that autophagy plays an indirect role in DENV replication by modulating cellular lipid metabolism. Relevant to DENV infection, a type of selective autophagy termed lipophagy was described, wherein autophagosomes can target cellular stores of lipids known as lipid droplets (LDs) to generate energy for the cell [38] . In subsequent work, the authors reproduced the published results that DENV induces and requires autophagy for robust viral replication [39] . These autophagosomes did not co-localize with markers of the viral replication complex, suggesting that they may play an indirect, non-structural role in DENV replication. Alternatively, DENV infection induces a selective autophagy that is preferentially targeted to lipid droplets, which leads to changes in cellular metabolism. More work, however, is required to show whether the proposed viral triggers of autophagy reproduce all cellular signals and phenotypes that accompany autophagy induction in DENV-infected cells.
cord-273065-peqz7okh	2020	
cord-273388-615acz0l	2016	
cord-274129-vaygaqe5	2015	
cord-276364-zyw5aukk	2019	Over the past few decades, a growing body of research has defined the critical role of this pathway in facilitating infection by numerous +RNA RNA viruses, including poliovirus (PV) [7, 8] , Coxsackievirus B3 (CVB3) [9, 10] , CVB4 [11] , Enterovirus 71 (EV71) [12] , Human rhinovirus (HRV) [13] , Foot-and-mouth disease virus (FMDV) [14] , encephalomyocarditis virus (EMCV) [15] , Dengue virus (DENV) [16, 17] , Zika virus (ZIKV) [18, 19] , Hepatitis C virus (HCV) [20] , Mouse hepatitic virus (MHV), Newcastle disease virus (NDV) [21] , Severe and acute respiratory syndrome coronavirus (SARS-CoV) [22] , Chikungunya virus (ChikV) [23] , and Japanese encephalitis virus (JEV) [24] . Delineating the process of viral assembly from replication is technically challenging, especially since both processes would very likely Induces formation of autophagosome-like double-membrane liposomes [112] Summary of Interactions between proteins from positive strand RNA viruses and host autophagy machinery.
cord-276718-3lujp0oy	2014	title: Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India In the present study, the standardization and validation of a one step, four tube reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for rapid detection and serotyping of the DENV targeting NS1 gene using the Genie® II flourometer was carried out. In the present study, the RT-LAMP assay was developed for the detection and serotyping of DENV infection targeting the serotype specific regions of the NS1 gene using a real-time flourometer (Genie ® II from Optigene, U.K.). The performance of the RT-LAMP assay was validated by testing the samples simultaneously by the CDC real time PCR that is most sensitive and specific method for detection and differentiation of the DENV (CDC Dengue). Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay
cord-277547-2vim1wno	2011	In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. Daidzein showed a weak anti-dengue activity with IC(50 )= 142.6 μg mL(-1 )when the DENV-2 infected cells were treated after virus adsorption. Although there was no significant direct virucidal activity against DENV-2 by quercetin, continuous treatment of cells from 5 h before virus infection up to 4 days post-infection exhibited anti-dengue activity with IC 50 = 28.9 μg mL -1 (Figure 3a) . There was no significant change in the antiviral activity of daidzein when cells were treated continuously from 5 h before virus infection up to 4 days post infection comparing to its anti-dengue activity for postadsorption treatment (Figure 1 ). To investigate which of the many flavonoids could affect DENV infection, in the present study, we examined the potential effects of quercetin, naringin, hesperetin and daidzein on dengue virus infection of Vero cells.
cord-278260-3o91v72a	2020	Within months large numbers of vaccinated children developed a severe breakthrough disease, called "atypical measles." [6] A similar outcome, "vaccine associated enhanced respiratory disease (VAERD)," was observed in infants, 4 -12 months of age, who were given formalininactivated respiratory syncytial virus (RSV) and a few months later infected by RSV. The biological behavior of some coronaviruses in non-human species together with evidence that human coronavirus antibodies enhanced infection of SARS or MERS CoVs in Fc receptor-bearing cells, in vitro, have led to speculations that ADE contributes to disease severity in humans. [11] It has been reported that high levels of SARS CoV-1 IgG antibodies circulated in severe SARS cases and that anti-S IgG neutralizing antibody (NAb) responses developed significantly faster after the onset of clinical symptoms in fatal compared with recovered cases leading some to attribute enhanced tissue damage to ADE. With others, we conclude that the differences in clinical, epidemiological and pathological features of SARS and DENV diseases suggest that iADE does not contribute to the severity of natural human coronavirus infections.
cord-282204-j1slaefb	2018	de; Oliveira, Renato A.S.; Durães-Carvalho, Ricardo; Lopes, Thaísa R.R.; Silva, Daisy E.A.; Gil, Laura H.V.G. title: A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control Laboratory tests for specific diagnosis of CHIKV infection are based on virus isolation, viral RNA detection and serology (Johnson et al., 2016) . Anti-CHIKV candidates that have been already tested in humans and/or animals include inactivated-, attenuated-, virus like particle-(VLP), DNA-and chimeric vaccines (Eckels et al., 1970; Levitt et al., 1986; Muthumani et al., 2008; Wang et al., 2008; Tiwari et al., 2009; Sharma et al., 2012 Akahata et al., 2010 Plante et al., 2011; Wang et al., 2011; Gorchakov et al., 2012; Brandler et al., 2013; Chang et al., 2014; García-Arriaza et al., 2014; Tretyakova et al., 2014; van den Doel et al., 2014; Erasmus et al., 2017) .
cord-282742-eyukbot7	2013	Geiss, Pierson, and Diamond (2005) observed that siRNAs targeting the C gene had no effect on virus replication when transfected into cells 10 h after WNV infection using lipid-based reagents. In addition, no significant reduction in viral protein or RNA levels was seen in WNV replicon-expressing cells transfected with siRNAs targeting the NS3 gene using lipid-based reagents. Also, a recent report showed that siRNA toward the TNF-a gene reduced cytokine response in DENV-infected DCs, highlighting the potential of targeted RNAi-based approaches to simultaneously decrease viral replication and the detrimental host immune response (Subramanya et al., 2010) . In addition, it has been shown that WNV (Chotkowski et al., 2008) and DENV (Mukherjee & Hanley, 2010) infection (Mukherjee & Hanley, 2010) of Drosophila cell lines induce functional virus-specific siRNAs that promote a protective RNAi response. So far we have described the antiviral effect of the RNAi mechanism induced by exogenous delivery of siRNA or precursors, and how cellular miRNA can target sequences artificially introduced within the genome of flaviviruses.
cord-285538-3ah05ijf	2014	3, 4 While follow-up research in the 1960s suggested that this phenomenon resulted from the stabilization of MVEV by antibodies, 5 a different explanation emerged when sequential infections in humans with dengue viruses (DENVs) were shown to produce severe disease (dengue hemorrhagic fever, DHF). Unexpectedly, the incubation of Ross River virus (RRV) with diluted RRV antiserum resulted in enhanced infection in mouse macrophage cell lines and in primary human monocytes/macrophages via innate immune suppression involving reduced production of reactive nitrogen radicals via NOS2 and a downregulation of TNF-α and IFN-β production through abolished interferon regulatory factor 1 (IRF-1) and nuclear factor-κB gene expression. 43 Note has been made above of the possible role of different Fcγ receptors in mediating ADE in macrophages in leishmania mouse models and the interesting observation suggesting that ligation of FcγRIIB may affect the fate of large immune complexes during the immune clearance phase of dengue infections.
cord-286255-ded5t1ai	2017	Blood and oro-nasopharyngeal specimens were tested by RT-PCR and immunodiagnostic methods for infection with dengue viruses (DENV) 1–4, chikungunya virus (CHIKV), influenza A and B viruses (FLU A/B), 12 other respiratory viruses (ORV), enterovirus, Leptospira spp., and Burkholderia pseudomallei. Clinical predictors of laboratory-positive dengue compared to all other AFI etiologies were determined by age and day post-illness onset (DPO) at presentation. By enrolling febrile patients at clinical presentation, we identified unbiased predictors of laboratory-positive dengue as compared to other common causes of AFI. Among 6,349 participants who presented early (<3 DPO) in the clinical course, leukopenia, thrombocytopenia, headache, eye pain, nausea, and dizziness were significant positive predictors of laboratory-positive dengue as compared to all other AFI cases across all age groups ( Table 5 ). Of note, 6% of participants !65 years old had dengue as a cause of AFI, a finding comparable to a Puerto Rico study in which 5% of 17,666 laboratory-positive dengue cases detected by surveillance were !65 years old [36] .
cord-290385-0smnl70i	2016	
cord-294842-aesiff1f	2014	
cord-295191-xu26mvc3	2008	
cord-297662-slmlhqnb	2017	In this review, we seek to provide a comprehensive summary of the current knowledge on protein glycosylation in DENV, and its role in virus biogenesis, host cell receptor interaction and disease pathogenesis. Since high mannose binding DC-SIGN interacts only with N67 glycans on the viral surface (Pokidysheva et al., 2006) and N153-glycan is dispensable for virus production in mosquito and mammalian cells (Bryant et al., 2007) , this suggests that N153 glycans may serve a distinct function from N67 glycans in DEN pathogenesis possibly via interaction with an unknown fucose binder or act as a viral glycan shield. Finally, N153 deglycosylated (N153 − ) DENV mutant displayed reduced infectivity (10-fold lower) in both mammalian and mosquito cells compared to WT, possibly due to impaired virus entry process (Lee et al., 1997; Hacker et al., 2009) , whereby loss of the N153-glycan affected the conformational stability of E proteins and led to premature exposure of the fusion peptide (Yoshii et al., 2013) . N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement
cord-300648-ixiam7qr	2014	
cord-303818-z3js3mr4	2015	
cord-306204-rkher4ly	2020	Vero cells grown in 24-well plates were infected with 30-50 FFU of DENV-2 in the presence or absence of BDTF, PDTF, methanol crude extracts, tannic acids of FT and ST at maximum non-toxic concentration (MNTC) of 100 µg/ mL, 50 µg/mL, 200 µg/mL, 50 µg/mL and 25 µg/mL respectively for 1 h at 4 °C. To study whether tannins in the porcupine dates are bioactive against dengue infection, we have isolated the tannin fractions from MBD and MPD for Besides that, both BDTF and PDTF demonstrated similar and higher virucidal activity against DENV-2 when compared to their methanol crude extracts. " indicates not significant Fig. 9 Effect of pre-treatment with porcupine dates extracts and tannic acids on DENV-2 infection in Vero cells. The antiviral activity ascribed to the interaction between the extracts and host cells was also investigated; pre-treating the cells with BDTF prevented the DENV-2 infection by at least 75% and similar result was observed for FT tannic acid.
cord-307219-okvvajms	2020	In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. She was investigated for West Nile virus, Usutu virus, dengue virus (DENV), chikungunya virus (CHIKV) and Zika virus (ZIKV) by molecular and serology testing, because she reported that a family member (Case 1) had had similar symptoms after a recent travel in Indonesia. The surveillance, active between June and November, aims to increase the detection rate of DENV and CHIKV (since 2015, also ZIKV) infection in travellers from endemic areas and to promptly identify potential autochthonous cases.
cord-317169-qlqavi4t	2015	(Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2). Certain flavonoids exhibited comparatively weaker antiviral activity, notably quercetin which could inhibit both MHV and DENV-2. This was followed by quercitrin which could inhibit DENV-2 but not MHV, whereas rutin did not exert any inhibitory effect on either virus. cordata contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in vivo. cordata exhibited anti-MHV activity at a MIC of 0.98 mg/mL without any apparent cytotoxic effects on CCL9.1 cells. cordata and its flavonoid component, quercetin, could inhibit both MHV and DENV-2 in vitro. Houttuynia cordata extracts and constituents inhibit the infectivity of dengue virus type 2 in vitro
cord-318120-vfznyyz6	2015	title: Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loopmediated isothermal amplification assay Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid and real-time detection of Japanese encephalitis virus
cord-318614-518giv0m	2019	A pan-dengue virus (DENV) RT-iiPCR, targeting the 5'' untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. CONCLUSIONS/SIGNIFICANCE: With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease. Testing with the pan-DENV RT-iiPCR, the analytical and clinical performance of the fully automatic POCKIT Central system was comparable to those of the semi-automatic POCKI combo system, which was validated previously to offer performances equivalent to the CDC DENV1-4 real-time RT-PCR for the detection of DENV in human serum [7, 24, 26] .
cord-319174-pbqjg7hf	2014	
cord-320091-2lrqubdl	2018	
cord-326840-piv6h7oq	2018	
cord-328661-spxgox52	2019	The lack of sufficient epidemiological data and evidence on the local mosquito-borne DENV emphasizes the importance of studying the molecular evolutionary features and establishing a well-established phylogenetic tree for dengue prevention and control in Guangdong. Since 1990, however, DENV1 has been mainly isolated from the infected cases, and its continued existence in Guangdong Province indicated that endemic infectious agents of dengue may be circulating locally. With the epidemiological data since 2005 supplied by the Guangdong Provincial CDC, we studied phylogenetics, molecular characteristics, and epidemiology to strengthen the foundational research of DENV1 for the prevention of large-scale dengue epidemics, providing preventive and control measures of DF with important evidence. Based on representative strains of the E gene in lineages of the 2014 outbreak, as well as the molecular evolution database, we analyzed molecular characterization and possibility of local circulation for DENV1 since 2005 in Guangdong.
cord-330743-o11d0aa1	2020	Herein, we identified 2 secreted bacterial lipases from a Chromobacterium bacterium, named Chromobacterium antiviral effector-1 (CbAE-1) and CbAE-2, with a broad-spectrum virucidal activity against dengue virus (DENV), Zika virus (ZIKV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus (HIV) and herpes simplex virus (HSV). Incubation of the culture supernatant but not the bacterial lysates resulted in significant suppression of DENV ( Figure 1B ) and ZIKV ( Figure 1C ) infectivity in Vero cells, indicating that an extracellular effector(s) secreted by Csp_BJ was responsible for viral inhibition. DENV, ZIKV, HSV-1 and SARS-CoV-2 virus stocks were diluted to 50 plaque-forming units (pfu) per ml and incubated untreated or with a serial dilution of the CbAEs in five-fold steps at 37°C for 1 hr before being added onto Vero cell monolayers for 2 hr of infection.
cord-342157-qjyooq68	2008	In our study, complete genomic sequencing of DENV-3 strains collected from different geographical locations and isolation years were determined and the sequence diversity as well as selection pressure sites in the DENV genome other than within the E gene were also analyzed. RESULTS: Using maximum likelihood and Bayesian approaches, our phylogenetic analysis revealed that the Taiwan''s indigenous DENV-3 isolated from 1994 and 1998 dengue/DHF epidemics and one 1999 sporadic case were of the three different genotypes – I, II, and III, each associated with DENV-3 circulating in Indonesia, Thailand and Sri Lanka, respectively. Compared to the prototype strain H87, several unique amino acid substitutions that serve as unique signature sites for each genotype were found within the full genomic sequences of the selected DENV-3 isolates from Taiwan or other countries and are listed by the order of the gene in Table 3 .
cord-344020-8poerd09	2020	We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. On August 27, upon confirmation of the serological results, Patient 1 was reported by the RIVM to the French authorities through the Early Warning and Response System of the European Union as an autochthonous DENV infection probably acquired in France with cross-border implication.
cord-345898-a6vt8kso	2016	There are three types of cell-based reporter systems that express certain reporter protein for positive-sense single strand RNA virus infections. An RVP is a type of virus-like particle (VLP) composed of viral structural proteins and a self-replicating replicon RNA containing a reporter gene [17, 63] . However, when the cells were infected with the specific virus, the viral RdRp was provided by the virus and the defective replicon was activated, resulting in high-level expression of the reporter gene, which could be easily examined [44] . However, when the cells were infected with the specific virus, the viral RdRp was provided by the virus and the defective replicon was activated, resulting in high-level expression of the reporter gene, which could be easily examined [44] . Development of Dengue type-2 virus replicons expressing GFP reporter gene in study of viral RNA replication
cord-353241-ityhcak7	2020	Considerable effort has been invested in the development of portable, user-friendly, and cost-effective systems for point-of-care (POC) diagnostics, which could also create an Internet of Things (IoT) for healthcare via a global network. Connecting the easy to use and cost-effective POC devices providing the DENV diagnoses via a mobile network would create an Internet of Things (IoT) [15] for healthcare [16, 17] , an essential tool to tackle any infectious disease outbreak. Prior to testing on an IoT PCR device, we verified the master mix performance and its values of critical threshold (C T ) and the melting temperature (T M ) using a commercial real-time PCR system (Supplementary Section A) beginning with a hot start at 95°C for 30 s followed by 40 cycles of PCR amplification consisting of DNA denaturation at 95°C for 8 s, primer annealing at 60°C for 30 s, and DNA sequence elongation at 72°C for 10 s, then followed by melting curve analysis (MCA) from 72°C to 95°C.
cord-355179-wmfwl2bh	2019	Overall, the data indicate that the antiviral activity of niclosamide during the early stage of the DENV life cycle correlates with the neutralization profile of the low-pH compartments, suggesting that blocking endosomal acidification results in the inhibition of viral genome replication and polyprotein processing, which further impedes viral protein expression and virus production. In this study, we found that neutralization of low-pH intracellular compartments by niclosamide not only inhibited the early stage of the DENV viral life cycle, such as viral RNA replication, independent of the entry step but also the late stage, specifically, the maturation of virus particles into infectious virions. Indeed, niclosamide treatment www.nature.com/scientificreports www.nature.com/scientificreports/ during the first 6 h of infection reduced DENV replication in BHK-21 cells harbouring dengue replicons to a level comparable to that of ribavirin, suggesting that the drug affects viral RNA replication and/or translation independent of its effect on entry, membrane fusion and genome release.
cord-355489-tkvfneje	2010	Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Due to the importance of DENV in public health, the particular goals of this research were to reconstruct the phylogenetic history of DENV-1 and to date the phylogenetic tree using isolation time as calibration points to establish date of introduction of virus and rate evolution patterns of virus in Colombia. Previously reported genotypes were represented in the tree and placed most of the Colombian isolates nesting in the genotype V clade (America, Africa) and were closely related to Argentina, Brazil and Paraguay virus strains.
cord-355610-7xy4s483	2019	
cord-355906-yeaw9nr8	2015