id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-355179-wmfwl2bh	Jung, Eunhye	Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro	2019-06-18		.txt	text/plain	5305	271	44	Overall, the data indicate that the antiviral activity of niclosamide during the early stage of the DENV life cycle correlates with the neutralization profile of the low-pH compartments, suggesting that blocking endosomal acidification results in the inhibition of viral genome replication and polyprotein processing, which further impedes viral protein expression and virus production. In this study, we found that neutralization of low-pH intracellular compartments by niclosamide not only inhibited the early stage of the DENV viral life cycle, such as viral RNA replication, independent of the entry step but also the late stage, specifically, the maturation of virus particles into infectious virions. Indeed, niclosamide treatment www.nature.com/scientificreports www.nature.com/scientificreports/ during the first 6 h of infection reduced DENV replication in BHK-21 cells harbouring dengue replicons to a level comparable to that of ribavirin, suggesting that the drug affects viral RNA replication and/or translation independent of its effect on entry, membrane fusion and genome release.	./cache/cord-355179-wmfwl2bh.txt	./txt/cord-355179-wmfwl2bh.txt