id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282797-thywse7g	Hwang, Yoon Jung	Engineered Bacteriophage T7 as a Potent Anticancer Agent in vivo	2020-09-24		.txt	text/plain	6166	337	55	We constructed an engineered bacteriophage T7 displaying a peptide, which targets murine melanoma cells and harbors a mammalian expression cassette of the cytokine granulocyte macrophage-colony stimulating factor (GM-CSF) in viral genomic DNA. It displayed peptides targeting mouse melanoma cells and at the same time harbored a mammalian expression cassette for the cytokine granulocyte macrophage-colony stimulating factor (GM-CSF). Engineered bacteriophage T7 displaying homing peptide (pep42) and expressing GM-CSF was added to the culture at an MOI of 100 and cells were incubated for 3 days. First and second row: wild type phage T7 (WT T7) or T7 displaying the homing peptide (T7-pep42) was added to in vitro cultured B16F10 melanoma cells and binding was observed under a fluorescent laser scanning confocal microscope. The engineered bacteriophage T7 displaying homing peptide (pep42) and harboring a mammalian expression cassette of murine GM-CSF was produced and the phage solution was nearly completely cleared (90%) of endotoxins ( Figure 1A) .	./cache/cord-282797-thywse7g.txt	./txt/cord-282797-thywse7g.txt