id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266499-g1lajsp8	Han, Jae-Ik	A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis	2015-09-21		.txt	text/plain	3077	163	44	title: A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis In the present study, a multiplex quantitative real-time polymerase chain reaction (mqPCR) panel was optimized for the detection of eight canine neurologic pathogens (Blastomyces dermatitidis, Cryptococcus spp., Neospora caninum, Borrelia burgdorferi, Bartonella spp., Toxoplasma gondii, Ehrlichia canis, and canine distemper virus [CDV]). The analytic sensitivity (i.e., limit of detection, expressed as molecules per 1 µL of recombinant vector) was 3.8 for CDV, 3.7 for Ehrlichia canis, 3.7 for Bartonella spp., 3.8 for Borrelia burgdorferi, 3.7 for Blastomyces dermatitidis, 3.7 for Cryptococcus spp., 38 for Neospora caninum, and 3.7 for Toxoplasma gondii. Recent studies have used multiplex quantitative real-time PCR (mqPCR) as a diagnostic tool for multifactorial diseases because this approach can simultaneously detect multiple pathogens with good diagnostic performance and is faster than conventional methods [8, 29] .	./cache/cord-266499-g1lajsp8.txt	./txt/cord-266499-g1lajsp8.txt