id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007665-vdtpz75u	Dörries, R.	Comparative analysis of virus-specific antibodies and immunoglobulins in serum and cerebrospinal fluid of subacute measles virus-induced encephalomyelitis (SAME) in rats and subacute sclerosing panencephalitis (SSPE)	2002-11-13		.txt	text/plain	4086	200	47	title: Comparative analysis of virus-specific antibodies and immunoglobulins in serum and cerebrospinal fluid of subacute measles virus-induced encephalomyelitis (SAME) in rats and subacute sclerosing panencephalitis (SSPE) The intrathecal humoral immune response was analysed in patients with subacute sclerosing panencephalitis (SSPE) and Lewis rats with subacute measles virus (MV)-induced encephalomyelitis (SAME). Although a restricted isoelectric pattern of MV-specific antibodies was detected in the cerebrospinal fluid (CSF) of SSPE patients as well as of SAME rats, the heterogeneity within clusters of immunoglobulin bands was higher in the rat specimens. A micro-enzyme immunoassay was used for determination of MV-specific titers in serum and CSF specimens from SSPE patients and diseased rats, MV antigen and Vero cell control antigen (25/~1, 100/tg protein/ml) were coated onto the surface of round-bottom microtiter plates (Immunoplates lI, Nunc, Wiesbaden, F.R.G.) by overnight incubation at room temperature in coating buffer (0.05 M sodium carbonate buffer, pH 9.6).	./cache/cord-007665-vdtpz75u.txt	./txt/cord-007665-vdtpz75u.txt