key: cord-317283-0zzs5cy8 authors: Nicoletti, Angela; Talarico, Valentina; Sabetta, Lucilla; Minchella, Pasquale; Colosimo, Manuela; Fortugno, Carmelo; Galati, Maria Concetta; Raiola, Giuseppe title: Screening of COVID-19 in children admitted to the hospital for acute problems: preliminary data date: 2020-05-11 journal: Acta Biomed DOI: 10.23750/abm.v91i2.9607 sha: doc_id: 317283 cord_uid: 0zzs5cy8 BACKGROUND: The new Coronavirus identified in Whuan at the end of 2019 (SARS-CoV-2) belongs to the Beta Coronavirus genus and is responsible for the new Coronavirus 2019 pandemia (COVID-19). Infected children may be asymptomatic or present fever, dry cough, fatigue or gastrointestinal symptoms. The CDC recommends that clinicians should decide to test patients based on the presence of signs and symptoms compatible with COVID-19. MATERIAL AND METHODS: 42 children (the majority < 5 years of age) were referred, to our Pediatric Department, as possible cases of COVID-19 infection. Blood analysis, chest X-ray, and naso-oropharyngeal swab specimens for viral identification of COVID-19 were requested. RESULTS: None of the screened children resulted positive for COVID-19 infection. At first presentation, the most frequent signs and symptoms were: fever (71.4%), fatigue (35.7%) and cough (30.9%). An high C-reactive protein value and abnormalities of chest X-ray (bronchial wall thickening) were detected in 26.2% and 19% of patients, respectively. Almost half of patients (45.2%) required hospitalization in our Pediatric Unit and one patient in Intensive Care Unit. CONCLUSIONS: Testing people who meet the COVID-19 suspected case definition criteria is essential for clinical management and outbreak control. Children of all ages can get COVID-19, although they appear to be affected less frequently than adults, as reported in our preliminary survey. Further studies are needed to confirm our observations. (www.actabiomedica.it) On 9 January 2020, a novel coronavirus, 2019-nCoV, was officially identified as the cause of an outbreak of diffuse pneumonitis in the city of Wuhan in Hubei Province, China. The epidemic has progressed very quickly in the following weeks, and an increasing number of cases have occurred daily in many countries (1) . The highest infection rate occurs in adults; however, neonates, children, and adolescents can also be infected (3) . People of all ages are susceptible to SARS-CoV-2 2019-nCoV infection (3) . The 95% confidence interval of 2019-nCoV infections period varies from 1 to 14 days (on average from 3 to 7 days). The majority of COVID-19 positive children had a close contact with an infected case or were family cluster cases and their age, at onset of disease, ranged from 1.5 months to 17 years (4) . Approximately 90% of cases are associated with household or community exposure, and 10 % are associated with travel. The clinical features are variable. Patients display no obvious clinical symptoms at diagnosis and are found by screening, because of close contacts with confirmed patients, or gradually present fever, fatigue, dry cough, accompanied by other upper respiratory symp-toms including nasal congestion, runny nose, and/or gastrointestinal symptoms, such as nausea, vomiting and diarrhoea (3) (4) (5) . From a systematic rieview of the literature, Ludvigsson (6) identified 45 relevant scientific papers and letters related to COVID-19 in children. The data showed a prevalence from 1 to 5%. The majority of them had a milder infection than in adults, although severe cases have been reported. Laboratory findings in children with confirmed infection are variable. Approximately one-quarter had white blood cell count <5.5 x 10 9 /L and 3.5 percent had lymphocyte count <1.2 x 10 9 /L (7). Procalcitonin was elevated ( >46 pg/mL) in 64 % and C-reactive protein was elevated (>10 mg/L) in 20 % of children. Chest radiographs may be unremarkable or demonstrate bilateral consolidation. In order to gain more insights into the outbreak of COVID-19, in our population of children and adolescents, we analyzed the clinical presentations, laboratory data and radiologic findings of patients who came to the Emergency Pediatric Department of Catanzaro (Italy), for an acute disease. The study was performed from 1 March 2020 to 13 April 2020. A total of 127 patients were enrolled in our survey. Demographic, clinical, and laboratory data of patients (age, sex, location, date at symptom onset, , travel history and information on relatives) were collected using a standardized electronic medical record. According to the international protocol, nasooropharyngeal swab specimens for the detection of 2019-nCoV were requested. A duplex one-step realtime reverse transcription polymeras chain reaction (RT-PCR), using specific target genes (E, RdRp, N), was used to confirm the 2019-nCoV positivity (https://www.who.int/emergencies/diseases/novel-coronavirus-2019/technical-guidance). Influenza virus A and B were routinely tested on respiratory swabs. Informed consent was obtained from the parents or guardians of children for the publication of clinical and laboratory data. The following variables: age, sex, clinical features, onset of symptoms, laboratory indices and imaging, were collected and reported as number, frequency (%), and median (interquartile range). Laboratory indices and chest X-ray descriptions, in our patients and in children and adolescents with COVID-19 infections reported in the literature, are also presented. Of the 127 children and adolescents admitted to the Pediatric Emergency room, 42 patients (20 males and 22 females) were suspected to have a COVID-19. Demographic data and clinical features of these patients are summarized in Table 1 . The median age was 6.2 years (1 months-17.8 yrs) and half of them (52.3%) were between 1 mo. to 5 yrs of age. A high personal risk history for suspected COVID-19 was present in five patients (11.9%). In three children a personal history of prematurity, bronchodysplasia or sickle cell anemia was reported. The average time of symptoms onset was 2.7 days. Fever was present in 71.4%, cough in 30.9%, and fatigue in 35.7% of our children and adolescents. Laboratory investigations and the reported radiologic findings are summarized in table 2. The most common abnormalities on chest X-ray were bronchial wall thickening in 8 patients (19%) and nodular or reticulonodular opacities in 2 patients (4.7%). 45.2% required hospitalization in our Pediatric Unit and 1 patient required intensive care support. None of our patients resulted positive for SARS-CoV-2 infection. The Centers for Disease Control and Prevention (CDC) and several other government health agencies emphasize that fever and respiratory symptoms are the criteria for suspecting a cases of 2019-nCoV infection. Based on the current WHO criteria (8) , patients are suspected to have an infection in pres-ence of the following epidemiological and clinical characteristics: a. Epidemiological history: 1) Children with a travel or residence history in an areas with persistent local transmission within 14 days prior to disease onset; 2) Children with a history of contacting patients with fever or respiratory symptoms who had a travel or residence history in an areas with persistent local transmission within 14 days prior to disease onset; 3) Children with a history of contacting confirmed or suspected cases infected with 2019-nCoV within 14 days prior to disease onset; 4) Newborns delivered by suspected or confirmed 2019-nCoV-infected mothers. b. Clinical presentations: 1) fever or respiratory symptoms (e.g.,cough, dypsnea, pharyngitis, flu-like symptoms) or digestive symptoms (e.g., vomiting, nausea and diarrhea) or fatigue. In Italy, the National Institute of Health (ISS; updated to 9 April 2020) reported 136,110 cases of COVID-19. The higher positive numbers were registered in the North of Italy (2). In Calabria Region 785 case of COVID-19 were registered, with an incidence rate of 40,32 per 100,000 of inhabitants (2) . Children represent the 2% of COVID-19 diagnosed cases in China (9), 1.2% of cases in Italy (10), and 5% of COVID-19 in the USA (11) . None of our tested children and adolescents for SARS-CoV-2 resulted positive. These results could be attributed to several factors: the low incidence of positive cases among children living in our Region (about 1.2% of the total registered in Italy); the early activation. in our region, of procedures and recommendations for reducing the viral transmission (e.g. schools closure, social distancing); to the different response of immune system in children (12) ; to the different environmental factors (13, 14) , and the absence of additional risk factors (such as: associated chronic diseases, smoking, air pollution exposure). Children often experience respiratory infections in winter, and may have higher levels of antibody against virus than adults. Furthermore, children's immune system is still developing, and may respond to pathogens differently to adults (11) . It has been also speculated that children are less sensitive to 2019-nCoV because the maturity and function (e.g., binding ability) of angiotensin converting enzyme II (ACE2) may be lower than that in adults (15) . ACE2 receptors are involved in the protective mechanisms of the lung to SARS-CoV infection, as indicated by recent evidence (16, 17) . Environmental factors, such as temperature, humidity, chemical and biological contaminants can play an important role in progression and spread of SARS-CoV infection (17, 18) . Higher temperatures and higher relative humidity (38°C, and >95% RH) have been found to reduce virus viability (18) . In addition, Oxygen saturation <94% -n. (%) 4 (9.5%) chemical and biological contaminants can promote the permanence and the spread of the virus, even over long distances (19) . In conclusion, testing people who meet the COV-ID-19 suspected case definition is essential for clinical management and outbreak control. The CDC recommends that clinicians should decide to test patients based on the presence of signs and symptoms compatible with COVID-19. An universal testing during the COVID-19 outbreak should be taken into consideration because is very important to understand the children's role in spreading the virus in their communities, to determine hospital isolation practices and bed assignments, to inform pediatric emergency care, and to guide the use of personal protective equipment. 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