key: cord-303860-jpy373ph authors: Huang, Zhifeng; Zhuang, Donglin; Xiong, Bing; Deng, David Xingfei; Li, Hanhua; Lai, Wen title: Occupational Exposure to SARS-CoV-2 in Burns Treatment During the COVID-19 Epidemic: Specific Diagnosis and Treatment Protocol date: 2020-04-23 journal: Biomed Pharmacother DOI: 10.1016/j.biopha.2020.110176 sha: doc_id: 303860 cord_uid: jpy373ph Epidemic prevention and control measures for the new coronavirus disease 2019 (COVID-19) has achieved significant results. As of 8 April 2020, 22,073 infection cases of COVID-19 among healthcare workers from 52 countries had been reported to WHO. COVID-19 has strong infectivity, high transmission speeds, and causes serious infection among healthcare worker. Burns are an acute-care condition, and burn treatment needs to be initiated before COVID-19 infection status can be excluded. The key step to infection prevention is to identify risk points of infection exposure, strengthen the protection against those risk points, and formulate an appropriate diagnosis and treatment protocol. Following an in-depth study of the latest literature on COVID-19 diagnosis and treatment, we reviewed the protocols surrounding hospitalization of patients with extensive burns (area≥50%) in our hospital from February 2009 to February 2019 and, in accordance with the epidemiological characteristics of COVID-19, developed an algorithm for protection during diagnosis and treatment of burns. Therefore, the aspects of medical protection and the diagnosis and treatment of burns appear to be particularly important during the prevention and control of the COVID-19. This algorithm was followed for 4 patients who received emergency treatment in February 2020 and were hospitalized. All healthcare worker were protected according to the three-tiered protective measures, and there was no nosocomial infection. During the COVID-19 epidemic, the early stages of emergency treatment for patients with extensive burns requiring the establishment of venous access for rehydration, endotracheal intubation or tracheostomy, wound treatment, and surgery are the risk points for exposure to infection. The implementation of effective, appropriate-grade protection and formulation of practical treatment protocols can increase protection of healthcare worke and reduce the risk of COVID-19 infection exposure. Since December 2019, a new type of coronavirus has continued to spread throughout the country [1] [2] . Globally, nearly 1.5 million confirmed cases of COVID-19 have now been reported to the World Health Organization (WHO) , and more than 92,000 deaths [3] .After initial virus-typing tests, the WHO officially named the new coronavirus causing the Wuhan pneumonia epidemic the "2019 new coronavirus (SARS-CoV-2)" on 12 January 2020. And the International Committee on Taxonomy of Viruses (ICTV) announced an official nomenclature of the new coronavirus (SARS-CoV-2) as the severe acute respiratory syndrome coronavirus 2 (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2). On the same day, the WHO announced that the official name of the disease caused by the virus is coronavirus disease 2019 (COVID-19) [4] . On 20 January 2020, China's "National Infectious Diseases Law" was amended to make COVID-19 a Class B notifiable disease and the management of a Class A infectious diseases has been adopted [5] . A WHO-China joint mission shared findings and recommendations on 25 February 2020, J o u r n a l P r e -p r o o f wherein 3,387 cases of COVID-19 was reported from among medical staff in 476 medical institutions in China, of whom 25 died [6] . As of 8 April 2020, 22,073 infection cases of COVID-19 among healthcare workers from 52 countries had been reported to WHO [7] . These data show that COVID-19 has strong infectivity, high transmission speed, and can cause serious infections among healthcare worker. Burns are an acute-care condition, and the treatment of patients with extensive burns poses a race against the time. Therefore, burn treatments need to be initiated before COVID-19 infection status can be excluded. The key step is to identify the risk points of infection exposure, strengthen the protection against those risk points, and formulate an appropriate diagnosis and treatment process. Improper protection can easily lead to the occurrence of medical infections. Therefore, the aspects of medical protection and the diagnosis and treatment of burns appear to be particularly important during the prevention and control of the COVID-19. To found the identify risk points of infection exposure, strengthen the protection against those risk points, and formulate an appropriate diagnosis and treatment protocol. Following an in-depth study of the latest literature on COVID-19 diagnosis and treatment, we reviewed the protocols surrounding hospitalization of patients with extensive burns in our hospital and, in accordance with the epidemiological characteristics of COVID-19, developed an algorithm for protection during diagnosis and treatment of burn patients. From 2009 to February 2019, we reviewed and analyzed the treatment conditions and protocols followed in patients with (area≥50%) burns patients Guangdong Provincial People's Hospital. To risk points of infection exposure during burn treatment according to the epidemiological characteristics of COVID-19, we obtained patient data, including the sex, age, diagnosis, admission time, operation time, endotracheal intubation or tracheotomy operation, central vein puncture operation, anesthesia mode, etc., from the medical records department of the hospital. Laboratory confirmation of the SARS-CoV-2 was achieved through the concerted efforts of the Guangdong Center for Disease Prevention and Control (CDC)，Guangzhou ardent clinical laboratory. The RT-PCR assay was conducted in accordance with the protocol established by the World Health Organization [8] . Quantitative variables were expressed as mean±SD whereas qualitative variables were presented as frequencies. The chi-square or Fisher's exact test was carried out for data analysis. A p-value <0.05 was considered statistically significant. 3.1.1. Source of infection: The source of infection seen so far is mainly patients with new type of coronavirus infection. Asymptomatic infection can also be a source of infection [9] . 3.1.2. Route of transmission: Respiratory droplets and close contact transmission are the main transmission routes. It is possible to propagate through aerosols when exposed to high concentration aerosols for a long time in a relatively closed environment. Because novel coronavirus can be isolated from feces and urine, attention should be paid to the spread of aerosols or contacts caused by environmental pollution [10] . The incubation period is 1-14 days, and is frequently 3-7 days. The main symptoms are fever, fatigue, and dry cough. A few patients have symptoms such as nasal congestion, runny nose, sore throat, and diarrhea. Severe patients often develop dyspnea and/or hypoxemia 1 week after disease onset and progress rapidly to acute respiratory distress syndrome (ARDS), septic shock, refractory metabolic acidosis, and coagulopathy. Notably, the course of severe and critically ill patients can be marked by moderate to low fever, or they may even be afebrile. Mildly affected patients show only low fever and mild fatigue without symptoms of pneumonia. Judging from the current cases, most patients have a good prognosis, and a few patients do become critically ill. The prognosis for the elderly and patients with chronic underlying disease is poor. Fortunately, symptoms in children are relatively mild. The peripheral white blood cell count in patients with early onset was normal or decreased, the lymphocyte count was normal or decreased, and some patients had elevated liver enzymes, muscle enzymes, and myoglobin; some critically ill patients showed increased troponin levels. In most patients, C-reactive protein and erythrocyte sedimentation rate (ESR) increased, and procalcitonin level was normal. In some patients, D-dimer increased and peripheral blood lymphocyte decreased progressively. Most severe and critically ill patients present showed elevated levels of inflammatory factors. SARS-CoV-2 was detected by reverse transcription polymerase chain reaction (RT-PCR) and/or next-generation sequencing (NGS) in nasopharyngeal swabs, sputum and other lower respiratory secretions, blood, and feces. The virus was more accurately detected in lower respiratory tract samples (sputum or airway extracts). Samples were sent for examination as soon as possible after J o u r n a l P r e -p r o o f collection. Most of the specific immunoglobulin M (IgM) antibodies of SARS-CoV-2 began to test positive 3-5 days after disease onset. The titer of IgG antibody in the recovery period was 4 times higher than that in the acute period. In the early stages, there were multiple, small, patchy shadows and interstitial changes on imaging, especially in the lateral zone of the lung. Later on, patients developed multiple ground-glass shadows and infiltrates in both lungs. In severe cases, lung consolidation may occur, although pleural effusion is rare. Any one of the epidemiological histories with any two of the clinical manifestations, or three of the clinical manifestations without epidemiological history are suspected cases. Details of the epidemiological history and clinical manifestations in patients with suspect COVID-19 are presented in Table 3 . Suspected cases were diagnosed as confirmed cases based on one of the following evidences: a. Laboratories" [11] , and developed an algorithm for the protection of healthcare worker during burn treatment. The above protective measures and treatment processes were carried out for 4 patients who required emergency treatment in February 2020 and were hospitalized ( Table 1) 2. During the COVID-19 epidemic, requirements for medical staff protection and a diagnosis and treatment flowchart for burn treatment were formulated ( Table 2) . As of 29 February 2020, the abovementioned protective measures and procedures were implemented for 4 patients suspected of COVID-19 infection in the emergency department. One patient was diagnosed as an asymptomatic carrier of the COVID-19 virus and was treated in the negative-pressure ward of the infectious diseases department. Two patients were suspected cases of COVID-19 and needed to be isolated for 14 days. CT examination of lung and tests for SARS-CoV-2 nucleic acid examination were negative when tested at two different timepoints. After the COVID-19 infection was ruled out, two patients were J o u r n a l P r e -p r o o f transferred back to the burn ward. One patient was considered free of COVID-19 infection and received treatment in the burn ward ( Table 3) . During the diagnosis and treatment of all four patients, all the healthcare worker carried out protective measures according to the three-level protection requirements, without contracting nosocomial infection. SARS-CoV-2 is highly infectious and pathogenic. COVID-19 is a new and sudden acute infectious disease that can be spread through person-to-person transmission. At present, COVID-19 has no significant known curative treatment. After the virus invades the human body, it mainly destroys the respiratory tract and causes pneumonia, which may lead to respiratory failure and endanger life [12] [13] [14] . In Wang's paper, as of 28 January 2020, of the 138 patients with COVID-19 in a general hospital at Wuhan, 57 (41.3%) were presumed to have been infected in the hospital, including 17 patients (12.3%) who were already hospitalized for other reasons and 40 healthcare workers (29%). Of the infected healthcare workers, 31 (77.5%) worked in the general wards, 7 (17.5%) in the emergency department, and 2 (5%) in the ICU [15] . More than 10 healthcare workers in the surgical department were presumed to have been infected by the first COVID-19 patient. Patient-to-patient and healthcare-worker-to-healthcare-worker transmission was presumed to have occurred. Therefore, the general department, especially the surgical department, is a department with high incidence of infection among healthcare worker in 476 medical institutions in China were reported, of which 25 died [6] . As of 8 April 2020, 22,073 infection cases of COVID-19 among healthcare workers from 52 countries had been reported to WHO [7] .These data show that COVID-19 has strong infectivity, high transmission speed, and can cause serious infection among healthcare worker. The lack of COVID-19 research in the early stages of the disease is one reason for healthcare worker to have neglected protection. In the middle stage of the disease, it was confirmed that COVID-19 is transmitted through respiratory droplets, close contact, and aerosol transmission. However, due to a large number of patients beyond Wuhan's urban medical allocation, the supply of medical protective materials is seriously insufficient, which leads to the continuous occurrence of nosocomial infections [17] . In fact, a large number of healthcare worker infected in a short period easily increase the work pressure and psychological pressures on other healthcare worker. Therefore, it is very important to identify the risk points of infection exposure, strengthen the protection of healthcare worker, and develop a standard protocol for the diagnosis and treatment. Patients with extensive burns need to receive a large amount of fluid resuscitation after being The function of the skin barrier is destroyed in patients with extensive burns, which leads to wound exposure and wound exudation. Early treatment requires topical drugs and wound dressings. This Fever is common in patients with extensive burns. Combined with respiratory tract burns, the patient can progress to have lung and bronchial injury. Because of systemic inflammatory response syndrome and large amount of rehydration treatment, burn patients are prone to pulmonary edema. X-ray examination of both lungs in burn patients showed multiple, small, spots and interstitial J o u r n a l P r e -p r o o f changes. This is very similar to the early chest-imaging findings of COVID-19. Therefore, it is not easy to differentiate and diagnose the two conditions clinically. In our study, 2 cases of large area burns were treated in emergency, 2 cases were febrile, and 1 case had multiple, small, spots on chest imaging. Quickly complete lung CT and blood routine examinations for new patients who are admitted to the emergency ward and screening, based on a new COVID-19-infected pneumonia diagnosis and treatment plan (Trial version 7) , should be combined with clinical symptoms and epidemiological history. Healthcare worker should establish secondary protective measures. As all new patients are routinely admitted to the emergency ward or the emergency ICU for regional isolation, the departmental healthcare worker should take primary protections. If it is necessary to perform invasive procedures in these patients, healthcare worker should undertake secondary protection. For the suspected or confirmed NCP patients, healthcare worker should strictly follow the emergency treatment plan, and promptly seek a consultation with an infectious diseases specialist, modify the treatment plan, and transfer the patient to the infectious diseases specialized ward. In cases where patients need to be treated with close contact, healthcare worker need to establish tertiary protection (Figure 1 ). Patients with extensive burns are prone to anasarca or neck edema leading to tracheal compression stenosis. When combined with respiratory burns, damage to airway mucosa, and tracheal edema, there is an increased possibility of asphyxia. Intubation or tracheostomy is often needed during early treatment in these patients. In this study, 1 burn patient needed endotracheal intubation and ventilator-assisted ventilation. Related literature suggests that medical procedures, such as endotracheal intubation, tracheotomy, mechanical ventilation, and fiberoptic bronchoscopy, can increase the risk of SARS-CoV and MERS-CoV transmission [18] . Therefore, according to the requirements of the WHO "Infection Prevention And Control During Health Care When Novel Coronavirus (NCoV) Infection Is Suspected" interim guidance and the opinions of the clinical expert committee, healthcare worker need to undertake tertiary protection (including hand hygiene, use of personal protective equipment, respiratory hygiene, needlestick prevention, cleaning of medical supplies, and treatment of medical waste, etc.) For suspected/confirmed patients of COVID-19, droplet isolation and contact isolation are required, and air-isolation measures need to be undertaken for medical procedures that can generate aerosols [19] . For patients who have not been confirmed free of COVID-19 infection, medical procedures can be carried out in the negative-pressure ward; alternatively, these procedures can be conducted in a well-ventilated ward to avoid operations in the positive-pressure ward. The authors declare that there are no conflicts of interest. Age and operation. In each area group of large burns, the number of men was more than that of women as jobs that men undertake may be more likely to cause burns than the jobs that women carry out. "+"Required; "-"Not required (except for exceptional circumstances); "±"Use as needed; "/"Insufficient protection; "+ *"Protective products for selective use / simultaneous use according to the actual conditions of medical institutions. "General diagnosis and treatment": This refers to non-aerosol-generating operations(Aerosol-generating operations include sputum suction, respiratory tract, and endotracheal intubation). c. Patients with fever or respiratory symptoms from Wuhan and surrounding areas, or from communities with reported cases within 14 days preceding illness onset. d. Aggregation onset (Two or more cases of fever and/or respiratory symptoms within the preceding 2 weeks in small areas, such as family, office, school class) a. Fever and/or respiratory symptoms b. With the above imaging characteristics of pneumonia c. Total number of white blood cells normal or decreased, or normal or decreased lymphocyte count in early-stage disease. Any one of the epidemiological histories with any two of the clinical manifestations, or three of the clinical manifestations without epidemiological history are suspected cases J o u r n a l P r e -p r o o f China Novel Coronavirus Investigating and Research Team, A novel coronavirus from patients with pneumonia in China Another decade, another coronavirus World Health Organization. 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