id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350855-gofzhff7	Hou, Yixuan J.	SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract	2020-05-27		.txt	text/plain	3416	222	50	High-sensitivity RNA in situ mapping revealed the highest ACE2 expression in the nose with decreasing expression throughout the lower respiratory tract, paralleled by a striking gradient of SARS-CoV-2 infection in proximal (high) vs distal (low) pulmonary epithelial cultures. COVID-19 autopsied lung studies identified focal disease and, congruent with culture data, SARS-CoV-2-infected ciliated and type 2 pneumocyte cells in airway and alveolar regions, respectively. We measured the relative infectivity of the SARS-CoV-2 GFP virus in primary 283 cells based on the average peak titers and observed that infectivity exhibited the same 284 pattern as the ACE2 expression levels from the upper to lower respiratory tract ( Figure 285 6Bi-6Biv). Gene 1230 expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human 1231 airway epithelial cells and lung tissue	./cache/cord-350855-gofzhff7.txt	./txt/cord-350855-gofzhff7.txt