id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348899-vynk8q8c	Jo, Seri	Inhibition of SARS-CoV 3CL protease by flavonoids	2019-11-14		.txt	text/plain	3989	236	54	A synthetic peptide labelled with an Edans-Dabcyl FRET (Fluorescence resonance energy transfer) pair 12 was used to search SARS-CoV 3CLpro inhibitory compounds against a flavonoid library. The proteolytic assay using the SARS-CoV 3CLpro in the presence of Triton X-100 has been performed to differentiate the artificial inhibitory activity of chemicals through non-specific binding with proteases by forming aggregate or complexation. The three compounds showed the severely reduced fluorescent intensity and thus represented their SARS-CoV 3CLpro inhibitory activity. The interactions between SARS-CoV 3CLpro and three inhibitory flavonoids were analysed to predict their binding affinities. We have created a library of flavonoids to systematically investigate SARS-CoV 3CLpro inhibitory compound by a FRET method. Herbacetin, rhoifolin and pectolinarin were the best inhibitory compounds against SARS-CoV 3CLpro in the flavonoid library. In order to predict the flavonoid scaffolds needed to interact with the catalytic site of SARS-CoV 3CLpro, an induced-fit docking study was performed and analysed.	./cache/cord-348899-vynk8q8c.txt	./txt/cord-348899-vynk8q8c.txt