id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301974-4wn40ivq	Berry, Jody D	Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus	2004-09-01		.txt	text/plain	5877	293	51	A total of 15 l of SARS-CoV antigen (infected Vero cell lysate) or 5 g of highly purified virus is coated (per spot) for 1 h at 37 • C. c Protein specificity tests shown here were determined by Western immunoblot with purified virus and infected cell lysate under denaturing conditions (Fig. 1) . The four Western immunoblot negative, virus-neutralising mAbs were tested for their ability to bind native SARS-CoV in infected cells by immunofluorescence assay. While purified virus is clearly the optimal antigen tested in this series of experiments, the lower quality SARS-CoV-infected Vero cell lysates are, however, much easier to prepare for diagnostic assays. This paper describes the development of murine mAbs which recognise SARS-CoV antigens in ELISA, immuno-dotblot, Western immunoblot, on the surface of infected cells, and in neutralisation assays.	./cache/cord-301974-4wn40ivq.txt	./txt/cord-301974-4wn40ivq.txt